| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-Hom-1 |
| 质粒类型: | 慢病毒载体；双顺反子载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 8478 bp |
| 5' 测序引物及序列: | CMV Forward： CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | DmrB (C、N-端均可） |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: |
慢病毒载体pLVX-Hom-1组成型表达DmrB融合蛋白； 外源基因与嘌呤霉素抗性基因以双顺反子的形式表达。 |
| 产品目录号: | 635073 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
pLVX-Hom-1载体质粒基本信息
pLVX-Hom-1质粒图谱载体图谱和pLVX-Hom-1载体序列质粒序列多克隆位点信息
pLVX-Hom-1质粒载体简介
pLVX-Hom-1质粒序列载体序列
LOCUS pLVX-Hom-1 8478 bp DNA circular 5-OCT-2011
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Catalog Nos. 635072, 635073
FEATURES Location/Qualifiers
LTR 1..635
/label=5'\LTR
misc_feature 685..822
/label=packaging\signal
misc_feature 2028..2151
/label=cPPT/CTS
misc_feature 4400..4991
/label=WPRE
rep_origin complement(6301..6971)
/label=pUC\ori
CDS complement(7116..8112)
/label=Amp(R)
misc_binding 1303..1536
/label=RRE
misc_binding 636..653
/label=PBS
LTR 5195..5831
/label=3'LTR
misc_feature 3179..3754
/label=IRES
CDS 3787..4386
/label=Puro(R)
misc_feature 2821..3153
/label=DmrB
misc_feature 2185..2787
/label=pCMV
BASE COUNT 2169 a 2111 c 2190 g 2008 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt
2821 atggcttcta gaggagtgca ggtggagact atctccccag gagacgggcg caccttcccc
2881 aagcgcggcc agacctgcgt ggtgcactac accgggatgc ttgaagatgg aaagaaagtt
2941 gattcctccc gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc
3001 cgaggctggg aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata
3061 tctccagatt atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact
3121 ctcgtcttcg atgtggagct tctaaaactg gaaagcggcc gcggatccta cgtatgaccg
3181 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
3241 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
3301 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
3361 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
3421 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
3481 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
3541 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
3601 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
3661 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
3721 ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccac aaggagacga
3781 ccttccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg
3841 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac
3901 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
3961 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
4021 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
4081 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
4141 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
4201 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
4261 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
4321 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
4381 gcctagacgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
4441 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
4501 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
4561 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
4621 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
4681 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
4741 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
4801 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
4861 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
4921 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
4981 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt
5041 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
5101 gtgggttttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
5161 agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg
5221 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
5281 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
5341 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
5401 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
5461 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
5521 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
5581 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
5641 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
5701 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
5761 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
5821 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
5881 gaatatcaga gagtgagagg ccttgacatt gctagcgttt taccgtcgac ctctagctag
5941 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
6001 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
6061 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
6121 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
6181 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
6241 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
6301 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
6361 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
6421 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
6481 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
6541 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
6601 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
6661 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
6721 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
6781 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
6841 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt
6901 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
6961 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
7021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
7081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
7141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
7201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
7261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
7321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
7381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
7441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
7501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
7561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
7621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
7681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
7741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
7801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
7861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
7921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
7981 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
8041 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
8101 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa
8161 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
8221 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
8281 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctagt
8341 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
8401 tttgttaaat atgtactaca aacttagtag tttttaaaga aattgtattt gttaaatatg
8461 tactacaaac ttagtagt
//
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