pGEM-5Zf(+)

价格:1500元

联系方式:I47-825O-882O

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pGEM5Zf(+)载体基本信息

出品公司: Promega
载体名称: pGEM-5Zf(+)
质粒类型: 克隆载体
高拷贝/低拷贝: 高拷贝
克隆方法: --
启动子: --
载体大小: 3000 bp
5' 测序引物及序列: M13 fwd
3' 测序引物及序列: M13 rev
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: --
宿主细胞(系): --
备注: 来源于pGEM-3Zf+载体,包含丝状噬菌体f1复制的起源;蓝白筛选
产品目录号: --
稳定性: --
组成型/诱导型: --
病毒/非病毒: 非病毒

pGEM5Zf(+)载体质粒图谱和多克隆位点信息

pGEM-5Zf(+)载体图谱
pGEM-5Zf(+) 载体图谱1

pGEM5Zf(+)载体简介

pGEM5Zf(+)载体序列

LOCUS       pGEM-5Zf(+)                3000 bp ds-DNA    circular SYN 24-6-2015
DEFINITION  Vector for standard cloning, efficient in vitro RNA synthesis, and 
            ssDNA production, with an MCS suitable for use with the 
            Erase-a-Base(TM) system.
ACCESSION   X65308
VERSION     .
KEYWORDS    pGEM-5Zf(+)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Promega
  TITLE     Direct Submission
  JOURNAL   Exported 2017/2/28 from SnapGene 2.3.2
            http://www.snapgene.com
COMMENT     
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    10..113
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(125..143)
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(161..177)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    185..201
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(209..239)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(563..1151)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1322..2182)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2183..2287)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      complement(2365..2820)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(2797..165)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /note="lacZ-alpha"
                     /translation="MTMITPSYLGDTIEYSSYASNALGALPYGRPAGGRTSDIPRPWRP
                     GACDVGPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRP
                     SQQLRSLNGEWTRPVAAH"
     primer_bind     2961..2977
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2984..2
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggccgcggga tatcactagt
       61 gcggccgcct gcaggtcgac catatgggag agctcccaac gcgttggatg catagcttga
      121 gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt
      181 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      241 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      301 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      361 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      421 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      481 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      541 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      601 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      661 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      721 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      781 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      841 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      901 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      961 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1021 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1081 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1141 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1201 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1261 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1321 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1381 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1441 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1501 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1561 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1621 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1681 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1741 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1801 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1861 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1921 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1981 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2041 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2101 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2161 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2221 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2281 ttccgcgcac atttccccga aaagtgccac ctgatgcggt gtgaaatacc gcacagatgc
     2341 gtaaggagaa aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt
     2401 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     2461 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     2521 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     2581 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     2641 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     2701 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     2761 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     2821 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2881 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2941 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
//

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pGEM5Zf(+)载体应用举例

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