| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pCMV-RFP-C-HA |
| 质粒类型: | 哺乳动物表达载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 4723 bp |
| 5' 测序引物及序列: | CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’ |
| 3' 测序引物及序列: | BGH rev 5''TAGAAGGCACAGTCGAGG3'' |
| 载体标签: | RFP、HA (C ter） |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 哺乳动物细胞系 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Exported bp ds-DNA circular SYN 12-AUG-2016
KEYWORDS pCMV-RFP-C-HA
REFERENCE 1 (bases 1 to 4723)
AUTHORS Biofeng Lab.
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..4723
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 67..370
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 371..574
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 625..643
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 650..652
/note="ATG"
misc_feature 659..696
/note="MCS"
gene 707..1399
/note="RFP"
CDS 1400..1426
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/note="HA"
/translation="YPYDVPDYA"
polyA_signal 1456..1581
/note="bGH poly(A)"
/note="bovine growth hormone polyadenylation signal"
rep_origin complement(1771..2077)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2121..2225
/gene="bla"
/note="bla promoter"
promoter 2425..2584
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2435..2570
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2619..3413
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3649..3692
/note="HSV TK poly(A)"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3998..4641
/direction=RIGHT
/note="pUC"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tcccgggagt aatcaattac ggggtcatta gttcatagcc catatatgga
61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
541 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
601 ttactggctt atcgaaattc tagataatac gactcactat agggcgaata tggccacggg
661 cgcgccctgc aggctagcct cgagggatcc gatatcgcgg ccgcttcttt ccaaacaagt
721 tttgcctcat gatgtgaaaa tgagatacca tatggatgga tgtgttaacg gtcacagttt
781 cactattgag ggagaaggtg ctggtaaacc ttatgaaggc aaaaagatct tggaactgcg
841 tgtaacaaag ggaggaccac ttccattcgc ctttgacatt ttgtccagcg ttttcactta
901 tggtaacagg tgtttctgtg agtatccaga ggatatgccc gactacttta agcaatcatt
961 gcccgagggt cactcttggg agagaacttt aatgtttgag gatggaggat gcggtactgc
1021 ttcagcccat atatcgttgg acaaaaactg tttcgtgcat aagtctacgt ttcatggtgt
1081 gaattttcct gctaatggtc cggtcatgca gaaaaaggca atgaattggg agcctagctc
1141 tgaactgatc accgcatgtg atggtattct caaaggcgac gttaccatgt ttttgttgct
1201 ggaaggtggt cacagattaa agtgccaatt cacaacatcc tacaaggctc ataaagcagt
1261 gaagatgcca ccaaatcaca ttatcgagca tgtcctagtc aaaaaggaag ttgctgacgg
1321 ttttcaaatc caggaacacg ctgttgcaaa acacttcaca gtagatgtta aggaaactcg
1381 cggccgcgaa ttcagatctt acccctacga cgtgcccgac tacgccgtta acttatgata
1441 aggccggcct gatcaaaaat cagcctcgac tgtgccttct agttgccagc catctgttgt
1501 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
1561 ataaaatgag gaaattgcat ctgtacatct agcattcagt tgtggtttgt ccaaactcat
1621 caatttatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
1681 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
1741 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
1801 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
1861 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
1921 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
1981 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
2041 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
2101 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
2161 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
2221 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
2281 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
2341 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
2401 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
2461 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
2521 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
2581 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
2641 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
2701 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
2761 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
2821 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
2881 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
2941 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
3001 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
3061 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
3121 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
3181 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
3241 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
3301 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
3361 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
3421 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
3481 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
3541 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
3601 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
3661 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
3721 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
3781 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
3841 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
3901 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
3961 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
4021 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
4081 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
4141 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
4201 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
4261 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
4321 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
4381 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
4441 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
4501 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
4561 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
4621 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
4681 gttatcccct gattctgtgg ataaccgtat taccgccatg cat
//
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