| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pCMV-RFP-C-Myc |
| 质粒类型: | 哺乳动物表达载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 4726 bp |
| 5' 测序引物及序列: | CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’ |
| 3' 测序引物及序列: | BGH rev 5''TAGAAGGCACAGTCGAGG3'' |
| 载体标签: | RFP、Myc (C ter） |
| 载体抗性: | kana |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 哺乳动物细胞 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pCMV-RFP-C-Myc载体质粒基本信息
pCMV-RFP-C-Myc质粒图谱载体图谱和pCMV-RFP-C-Myc载体序列质粒序列多克隆位点信息
pCMV-RFP-C-Myc质粒载体简介
pCMV-RFP-C-Myc质粒序列载体序列
LOCUS Exported 4726 bp ds-DNA circular SYN 12-AUG-2016
KEYWORDS pCMV-RFP-C-Myc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4726)
AUTHORS Biofeng Lab.
FEATURES Location/Qualifiers
source 1..4726
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 67..370
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 371..618
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 625..643
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 650..652
/note="ATG"
misc_feature 659..696
/note="MCS"
gene 707..1399
/note="RFP"
CDS 1400..1429
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
polyA_signal 1459..1584
/note="bGH poly(A)"
/note="bovine growth hormone polyadenylation signal"
rep_origin complement(1642..2097)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2124..2228
/gene="bla"
/note="AmpR promoter"
promoter 2328..2587
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2438..2573
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2622..3416
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3648..3695
/note="HSV TK poly(A)"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4001..4644
/direction=RIGHT
/note="pUC ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tcccgggagt aatcaattac ggggtcatta gttcatagcc catatatgga
61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
541 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
601 ttactggctt atcgaaattc tagataatac gactcactat agggcgaata tggccacggg
661 cgcgccctgc aggctagcct cgagggatcc gatatcgcgg ccgcttcttt ccaaacaagt
721 tttgcctcat gatgtgaaaa tgagatacca tatggatgga tgtgttaacg gtcacagttt
781 cactattgag ggagaaggtg ctggtaaacc ttatgaaggc aaaaagatct tggaactgcg
841 tgtaacaaag ggaggaccac ttccattcgc ctttgacatt ttgtccagcg ttttcactta
901 tggtaacagg tgtttctgtg agtatccaga ggatatgccc gactacttta agcaatcatt
961 gcccgagggt cactcttggg agagaacttt aatgtttgag gatggaggat gcggtactgc
1021 ttcagcccat atatcgttgg acaaaaactg tttcgtgcat aagtctacgt ttcatggtgt
1081 gaattttcct gctaatggtc cggtcatgca gaaaaaggca atgaattggg agcctagctc
1141 tgaactgatc accgcatgtg atggtattct caaaggcgac gttaccatgt ttttgttgct
1201 ggaaggtggt cacagattaa agtgccaatt cacaacatcc tacaaggctc ataaagcagt
1261 gaagatgcca ccaaatcaca ttatcgagca tgtcctagtc aaaaaggaag ttgctgacgg
1321 ttttcaaatc caggaacacg ctgttgcaaa acacttcaca gtagatgtta aggaaactcg
1381 cggccgcgaa ttcagatctg agcagaagct gatctccgag gaggacctgg ttaacttatg
1441 ataaggccgg cctgatcaaa aatcagcctc gactgtgcct tctagttgcc agccatctgt
1501 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
1561 ctaataaaat gaggaaattg catctgtaca tctagcattc agttgtggtt tgtccaaact
1621 catcaattta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
1681 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
1741 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
1801 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
1861 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
1921 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
1981 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
2041 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
2101 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
2161 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
2221 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
2281 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
2341 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
2401 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
2461 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
2521 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
2581 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
2641 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
2701 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
2761 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
2821 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
2881 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
2941 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
3001 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
3061 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
3121 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
3181 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
3241 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
3301 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
3361 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
3421 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
3481 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
3541 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
3601 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
3661 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
3721 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
3781 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
3841 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
3901 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
3961 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
4021 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
4081 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
4141 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
4201 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
4261 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
4321 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
4381 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
4441 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
4501 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
4561 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
4621 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
4681 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//
pCMV-RFP-C-Myc载体图谱质粒图谱pdf版和pCMV-RFP-C-Myc载体序列质粒序列等相关资料下载
pCMV-RFP-C-Myc质粒载体应用举例
- 上一篇：pCMV-RFP-C-HA
- 下一篇：pCMV-SPORT4
