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LOCUS pMXs-miR-GFP-Puro 7001 bp ds-DNA circular SYN 06-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 14
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7001)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, September 6, 2016 from SnapGene Viewer 3.1.4
http://www.biofeng.com/
FEATURES Location/Qualifiers
source 1..7001
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 22..615
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 678..1035
/note="MMLV Psi"
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1094..1510
/note="gag (truncated)"
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
misc_feature 1926..2042
/note="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2265..2476
/note="EF-1-alpha core promoter"
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 2995..3711
/codon_start=1
/product="enhanced GFP"
/note="EGFP"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 3718..4317
/codon_start=1
/gene="pac from Streptomyces"
/product="puromycin N-acetyltransferase"
/note="PuroR"
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
LTR 4506..4975
/note="long terminal repeat from Moloney murine leukemia
virus"
rep_origin complement(5276..5864)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6028..6888)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6889..6993)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acctggcacg
1861 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctcc
1921 ccaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
1981 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt
2041 ttatcgatgc ctccccgtca ccaccccccc caacccgccc cgaccggagc tgagagtaat
2101 tcatacaaaa ggactcgccc ctgccttggg gaatcccagg gaccgtcgtt aaactcccac
2161 taacgtagaa cccagagatc gctgcgttcc cgccccctca cccgcccgct ctcgtcatca
2221 ctgaggtgga gaagagcatg cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc
2281 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
2341 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
2401 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
2461 tgccgccaga acacaggtac acatattgac caaatcaggg taattttgca tttgtaattt
2521 taaaaaatgc tttcttcttt taatatactt ttttgtttat cttatttcta atactttccc
2581 taatctcttt ctttcagggc aataatgata caatgtatca tgcctctttg caccattcta
2641 aagaataaca gtgataattt ctgggttaag gcaatagccg attagttctc gaggatccga
2701 ctgaagtcgc tagctcgagc ttttggagaa tatttctgca tataaatatt tctgcatata
2761 aattgtaact gatgtaagag gtttcatatt gctaatagca gctacaatcc agctaccatt
2821 ctgcttttat tttatggttg ggataaggct ggattattct gagtccaagc taggcccttt
2881 tgctaatcat gttcatacct cttatcttcc tcccacagct cctgggcaac gtgctggtct
2941 gtgtgctggc ccatcacttt ggcaaagcac gtgagatctg aattctgaca caccatggtg
3001 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
3061 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
3121 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
3181 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
3241 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
3301 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
3361 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
3421 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
3481 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
3541 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
3601 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
3661 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggccaccatg
3721 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
3781 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
3841 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
3901 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
3961 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
4021 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
4081 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
4141 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
4201 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
4261 gtcgagtgcc cgaaggaccg cgcgacctgg tgcatgaccc gcaagcccgg tgcctgagcg
4321 gccgccagca cagtggtcga cgataaaata aaagatttta tttagtctcc agaaaaaggg
4381 gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa
4441 ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag gaacagatgg
4501 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
4561 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
4621 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
4681 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
4741 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
4801 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
4861 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
4921 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcacatgc
4981 agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt
5041 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
5101 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
5161 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
5221 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
5281 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
5341 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
5401 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
5461 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
5521 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
5581 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
5641 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
5701 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
5761 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
5821 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
5881 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
5941 gattatcaaa aaggatcttc acctagatcc ttttgcggcc ggccgcaaat caatctaaag
6001 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
6061 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
6121 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
6181 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
6241 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
6301 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
6361 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
6421 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
6481 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
6541 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
6601 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
6661 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
6721 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
6781 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
6841 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
6901 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
6961 tatttagaaa aataaacaaa taggggttcc gcgcacattt c
//
