| 出品公司: | Clonetech, Biofeng |
|---|---|
| 载体名称: | pBacPAK8-His |
| 质粒类型: | 昆虫表达载体；杆状病毒表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | Polyhedrin |
| 载体大小: | 5579 kb |
| 5' 测序引物及序列: | Bac1 Primer: AACCATCTCGCAAATAAATA |
| 3' 测序引物及序列: | Bac2 Primer: ACGCACAGAATCTAGCGCTT |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 真核筛选标记: | 无 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 果蝇细胞系 IPLB-Sf21 |
| 备注: | 相关载体：pBacPAK8 |
| 产品目录号: | BF593 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 杆状病毒 |
买家导航
pBacPAK8-His载体质粒基本信息
pBacPAK8-His质粒图谱载体图谱和pBacPAK8-His载体序列质粒序列多克隆位点信息
pBacPAK8-His质粒载体简介
pBacPAK8-His质粒序列载体序列
LOCUS pBacPAK8-His 5579 bp DNA circular 24-JUL-2016
DEFINITION pBacPAK8-His
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pBacPAK8-His|
FEATURES Location/Qualifiers
source 1..5579
/organism="pBacPAK8"
/mol_type="other DNA"
/vntifkey="98"
misc_feature 1..1147
/vntifkey="21"
/label=Ac_5_flank
promoter 1148..1248
/vntifkey="30"
/label=PH_promoter
misc_feature 1174..1191
/vntifkey="21"
/label=polyhedrin_fwd_primer
misc_feature 1378..2811
/vntifkey="21"
/label=Ac_3_flank
rep_origin 2899..3205
/vntifkey="33"
/label=f1_origin
misc_feature 3473..3495
/vntifkey="21"
/label=pGEX_3_primer
promoter 3654..3682
/vntifkey="30"
/label=AmpR_promoter
gene 3724..4584
/gene="Ampicillin"
/vntifkey="60"
CDS 3724..4584
/vntifkey="4"
/label=Amp\Resistance
rep_origin 4739..5358
/vntifkey="33"
/label=pBR322_origin
misc_feature 1259..1276
/vntifkey="21"
/label=His\Tag
BASE COUNT 1459 a 1217 c 1287 g 1616 t
ORIGIN
1 aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag
61 aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa
121 caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt
181 accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt
241 ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca
301 cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa
361 accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
421 caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt
481 gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
541 gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
601 ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
661 tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat
721 ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
781 ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
841 tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
901 ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
961 acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
1021 aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
1081 aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
1141 aatatatagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata
1201 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata ccatgggtca
1261 tcatcatcac catcacggtg gtcgacaagc ttggatccct gcaggcctcg agttcgaatc
1321 tagaagatct ggtaccgagc tcgaattccc gggcggccgc ttaattaatt gatccgggtt
1381 attagtacat ttattaagcg ctagattctg tgcgttgttg atttacagac aattgttgta
1441 cgtattttaa taattcatta aatttataat ctttagggtg gtatgttaga gcgaaaatca
1501 aatgattttc agcgtcttta tatctgaatt taaatattaa atcctcaata gatttgtaaa
1561 ataggtttcg attagtttca aacaagggtt gtttttccga accgatggct ggactatcta
1621 atggattttc gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat ctagctttgt
1681 cgatattcgt ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa atattatgcg
1741 cttttgtatt tctttcatca ctgtcgttag tgtacaattg actcgacgta aacacgttaa
1801 ataaagcttg gacatattta acatcgggcg tgttagcttt attaggccga ttatcgtcgt
1861 cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga ttttgccata gccacacgac
1921 gcctattaat tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag ctttttggaa
1981 ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct aactgtgccc gattttaatt
2041 cagacaacac gttagaaagc gatggtgcag gcggtggtaa catttcagac ggcaaatcta
2101 ctaatggcgg cggtggtgga gctgatgata aatctaccat cggtggaggc gcaggcgggg
2161 ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc agacggcggt ttaggctcaa
2221 atgtctcttt aggcaacaca gtcggcacct caactattgt actggtttcg ggcgccgttt
2281 ttggtttgac cggtctgaga cgagtgcgat ttttttcgtt tctaatagct tccaacaatt
2341 gttgtctgtc gtctaaaggt gcagcgggtt gaggttccgt cggcattggt ggagcgggcg
2401 gcaattcaga catcgatggt ggtggtggtg gtggaggcgc tggaatgtta ggcacgggag
2461 aaggtggtgg cggcggtgcc gccggtataa tttgttctgg tttagtttgt tcgcgcacga
2521 ttgtgggcac cggcgcaggc gccgctggct gcacaacgga aggtcgtctg cttcgaggca
2581 gcgcttgggg tggtggcaat tcaatattat aattggaata caaatcgtaa aaatctgcta
2641 taagcattgt aatttcgcta tcgtttaccg tgccgatatt taacaaccgc tcaatgtaag
2701 caattgtatt gtaaagagat tgtctcaagc tcggatcgat cccgcacgcc gataacaagc
2761 cttttcattt ttactacagc attgtagtgg cgagacactt cgctgtcgtc gcctgatgcg
2821 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
2881 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
2941 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
3001 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
3061 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
3121 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
3181 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
3241 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
3301 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
3361 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
3421 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
3481 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
3541 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
3601 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
3661 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
3721 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
3781 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
3841 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
3901 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
3961 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
4021 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
4081 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
4141 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
4201 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
4261 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
4321 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
4381 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
4441 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
4501 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
4561 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
4621 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
4681 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
4741 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
4801 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
4861 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
4921 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
4981 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
5041 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
5101 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
5161 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
5221 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
5281 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
5341 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
5401 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
5461 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
5521 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcag
//
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