| 质粒名称: | SETD2-GFP |
|---|---|
| 出品公司: | addgene |
| 目录编号: | 80653 |
| 存储实验室: | Sérgio De Almeida |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 11030 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | SETD2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 6400bp |
| 启动子: | CMV |
| 标签/融合蛋白: | EGFP (N terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BglII(未破坏) |
| 3’克隆位点: | PstI(未破坏) |
| 5’测序引物 | EGFP-C |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | |
| 参考文献部分: | |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS SETD2-GFP 11030 bp ds-DNA circular SYN 05-JAN-2023
DEFINITION mammalian expression of SETD2-GFP.
ACCESSION .
VERSION .
KEYWORDS SETD2-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11030)
AUTHORS Carvalho S, Vitor AC, Sridhara SC, Martins FB, Raposo AC, Desterro
JM, Ferreira J, de Almeida SF
TITLE SETD2 is required for DNA double-strand break repair and activation
of the p53-mediated checkpoint.
JOURNAL Elife. 2014 May 6;3:e02482. doi: 10.7554/eLife.02482.
PUBMED 24843002
REFERENCE 2 (bases 1 to 11030)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jan 5, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..11030
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 21..324
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 325..528
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 479..499
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 567..576
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 573..1289
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 573..1289
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(618..639)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(879..898)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1226..1247
/label=EGFP-C
/note="EGFP, forward primer"
CDS 7111..7122
/codon_start=1
/product="WELQut protease recognition and cleavage site"
/label=WELQut site
/translation="WELQ"
polyA_signal 7778..7899
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7815..7834)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7869..7888
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(7906..8361)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8043..8064)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 8255..8274
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 8388..8492
/gene="bla"
/label=AmpR promoter
promoter 8494..8851
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(8517..8537)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 8702..8837
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8764..8783
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 8886..9680
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(8940..8959)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 9550..9569
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(9868..9887)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 9912..9959
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 10288..10876
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 10777..10796
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
61 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
121 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
181 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
241 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
301 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
361 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
421 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
481 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
541 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca
601 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
661 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
721 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
781 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
841 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
901 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
961 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
1021 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
1081 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
1141 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
1201 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
1261 tcactctcgg catggacgag ctgtacaagt ccggactcag atctatggaa agaagaggca
1321 agtattcttc aaaactagaa agagaatcta aaaggacttc agaaaatgaa gcaattaaaa
1381 gatgttgttc tccccctaat gaactgggat tccgacgagg gtcatcatat tctaagcatg
1441 acagtagtgc ttcccgttat aaatctaccc tttcaaaacc tatacccaag tctgataaat
1501 ttaaaaattc tttctgttgt acagaattaa atgaagaaat caaacagtct cattctttta
1561 gtttacagac accttgttca aaaggtagtg aattaagaat gattaataaa aatcctgaaa
1621 gagaaaaggc tgggtctcca gctccatcaa atcgattaaa tgattcacct actttaaaaa
1681 agctagatga attgcctatt tttaagtccg aatttataac acatgatagc catgatagta
1741 ttaaggaatt agactcttta tctaaagtga agaatgatca attaagaagt ttttgtccca
1801 tagaattaaa tataaatgga tctcctgggg cagaatctga tttggcaaca ttttgcactt
1861 ctaaaactga tgctgtttta atgacttctg atgatagtgt gactggatcg gaattatccc
1921 ctttggtcaa agcatgcatg ctttcatcaa atggatttca gaatattagt aggtgcaaag
1981 aaaaagactt ggatgatacc tgcatgctgc ataagaagtc agaaagccca tttagagaaa
2041 cagaacctct ggtgtcacca caccaagata aactcatgtc tatgccagtt atgactgtgg
2101 attattccaa aacagtagtt aaagaaccag ttgatacgag ggtttcttgc tgcaaaacca
2161 aagattcaga catatactgt actttgaacg atagcaaccc ttctttgtgt aactctgaag
2221 ctgaaaatat tgagccttca gttatgaaga tttcttcaaa tagctttatg aatgtgcatt
2281 tggaatcaaa accagttata tgtgatagta gaaatttgac agatcactca aaatttgcat
2341 gtgaagaata taagcagagc atcggtagca ctagttcagc ttctgttaat cattttgatg
2401 atttatatca acctattggg agttcaggta ttgcttcatc tcttcagagt cttccaccag
2461 gaataaaggt ggacagtcta actctcttga aatgcggaga gaacacatct ccagttctgg
2521 atgcagtgct aaagagtaaa aaaagttcag agtttttaaa gcatgcaggg aaagaaacaa
2581 tagtagaagt aggtagtgac cttcctgatt caggaaaggg atttgcttcc agggagaaca
2641 ggcgtaataa tgggttatct gggaaatgtt tgcaagaggc tcaagaagaa gggaattcca
2701 tattgcctga aagaagagga agaccagaaa tctctttaga tgaaagagga gaaggaggac
2761 atgtgcatac ttctgatgac tcagaagttg tattttcttc ttgtgatttg aatttaacca
2821 tggaagacag tgatggtgta acttatgcat taaagtgtga cagtagtggt catgccccag
2881 aaattgtgtc tacagttcat gaagattatt ctggctcttc tgaaagttca aatgatgaaa
2941 gtgattcaga agatacagat tcggatgata gcagtattcc aagaaaccgt ctccagtctg
3001 ttgtggttgt gccaaagaat tctactttgc ccatggaaga aacaagtcct tgttcttctc
3061 ggagcagtca aagttataga cactattctg accattggga agatgagaga ttggagtcaa
3121 ggagacattt gtatgaggaa aaatttgaaa gtatagcaag taaagcctgt cctcaaactg
3181 ataagttttt ccttcataaa ggaacagaga agaatccgga aatttctttt acacagtcca
3241 gtagaaaaca aatagataac cgcctgcctg aactttctca tcctcagagt gatggggttg
3301 atagtacaag tcatacagat gtgaaatctg accctctggg tcacccaaat tcagaggaaa
3361 ccgtgaaagc caaaatacct tctaggcagc aagaagagct gccaatttat tcttctgatt
3421 ttgaagatgt cccaaataag tcttggcaac agaccacttt ccaaaacagg ccagatagta
3481 gactgggaaa aacagaattg agtttttctt cctcttgtga gataccacat gtggatggct
3541 tgcactcatc agaagagctc agaaacttag gttgggactt ctctcaagaa aagccttcta
3601 ccacgtatca gcaacctgac agtagctatg gagcttgtgg tggacacaag tatcagcaaa
3661 atgcagaaca gtatggtggg acacgtgatt actggcaagg caatggttac tgggatccaa
3721 gatcaggtag acctcctgga actggggttg tgtatgatcg aactcaagga caagtaccag
3781 attccctaac agatgatcgt gaagaagagg agaattggga tcaacaggat ggatcccatt
3841 tttcagacca gtccgataaa tttcttctat cccttcagaa agacaagggg tcagtgcaag
3901 cacctgaaat aagcagcaat tccattaagg acactttagc tgtgaatgaa aagaaagatt
3961 tttcaaaaaa cttagaaaaa aatgatatca aagatagagg gcctcttaaa aaaaggaggc
4021 aggaaataga gagtgattct gaaagtgatg gtgagcttca ggacagaaag aaagttagag
4081 tggaggtaga gcagggagag acatcagtgc ccccaggttc agcactggtt gggccctcct
4141 gtgtcatgga tgacttcagg gacccacagc gatggaagga atgtgccaag caagggaaaa
4201 tgccatgtta ctttgatctt attgaagaaa atgtttattt aacagaaaga aagaagaata
4261 aatctcatcg agatattaag cgaatgcagt gtgagtgtac acctctttct aaagatgaaa
4321 gagctcaagg tgaaatagca tgtggggaag attgtcttaa tcgtcttctc atgattgaat
4381 gttcttctcg gtgtccaaat ggggattatt gttccaatag acggtttcag agaaaacagc
4441 atgcagatgt ggaagtcata ctcacagaaa agaaaggctg gggcttgaga gctgccaaag
4501 accttccttc gaacaccttt gtcctagaat attgtggaga ggtactcgat cataaagagt
4561 ttaaagctcg agtgaaggag tatgcacgaa acaaaaacat ccattactat ttcatggccc
4621 tgaagaatga tgagataata gatgccactc aaaaaggaaa ttgctctcgt ttcatgaatc
4681 acagctgtga accaaattgt gaaacccaaa aatggactgt gaacggacaa ctgagggttg
4741 ggttttttac caccaaactg gttccttcag gctcagagtt aacgtttgac tatcagttcc
4801 agagatatgg aaaagaagcc cagaaatgtt tctgcggatc agccaattgc cggggttacc
4861 tgggaggaga aaacagagtc agcatcagag cagcaggagg gaaaatgaag aaggaacgat
4921 ctcgtaagaa ggattcagtg gatggagagc tagaagctct gatggaaaat ggtgagggtc
4981 tctctgataa aaaccaggtg ctcagcttat cccggctaat ggttagaatt gaaactttgg
5041 agcagaaact tacctgtctg gaactcatac agaacacaca ctcacagtcc tgcctgaagt
5101 cctttctgga acgtcatggg ctgtctttgt tgtggatctg gatggcagag ctaggtgacg
5161 gccgggaaag taaccagaag cttcaggaag agattataaa gactttggaa cacttgccca
5221 ttcctactaa aaatatgttg gaggaaagca aagtacttcc aattattcaa cgctggtctc
5281 agactaagac tgctgtccct ccgttgagtg aaggagatgg gtattctagt gagaatacat
5341 cgcgtgctca tacaccactc aacacacctg atccttccac caagctgagc acagaagctg
5401 acacagacac tcccaagaaa ctaatgtttc gcagactgaa aattataagt gaaaatagca
5461 tggacagtgc aatctctgat gcaaccagtg agctagaagg caaggatggc aaagaggatc
5521 ttgatcaatt agaaaatgtc cctgtagagg aagaggaaga attgcagtca caacagctac
5581 tcccacaaca gctgcctgaa tgcaaagttg atagtgaaac caacatagaa gctagtaagc
5641 tacctacatc tgaaccagaa gctgacgctg aaatagagct caaagagagc aacggcacaa
5701 aactagaaga acctattaat gaagaaacac catcccaaga tgaagaggag ggtgtgtctg
5761 atgtggagag tgaaaggagc caagaacagc cagataaaac agtggatata agtgatttgg
5821 ccaccaaact cctggacagt tggaaagacc taaaggaggt atatcgaatt ccaaagaaaa
5881 gtcaaactga aaaggaaaac acaacaactg aacgaggaag ggatgctgtt ggcttcagag
5941 atcaaacacc tgccccgaag actcctaata ggtcaagaga gagagaccca gacaagcaaa
6001 ctcaaaataa agagaaaagg aaacgaagaa gctccctctc accaccctct tctgcctatg
6061 agcggggaac aaaaaggcca gatgacagat atgatacacc aacttctaaa aagaaagtac
6121 gaattaaaga ccgcaataaa ctttctacag aggaacgccg gaagttgttt gagcaagagg
6181 tggctcaacg ggaggctcag aaacaacagc aacagatgca gaacctggga atgacatcac
6241 cactgcccta tgactctctt ggttataatg ccccgcatca tccctttgct ggttacccac
6301 caggttatcc catgcaggcc tatgtggatc ccagcaaccc taatgctgga aaggtgctcc
6361 tgcccacacc cagcatggac ccagtgtgtt ctcctgctcc ttatgatcat gctcagccct
6421 tggtgggaca ttctacagaa cccctttctg cccctccacc agtaccagtg gtgccacatg
6481 tggcagctcc tgtggaagtt tccagttccc agtatgtggc ccagagtgat ggtgtagtac
6541 accaagactc cagcgttgct gtcttgccag tgccggcccc cggcccagtt cagggacaga
6601 attatagtgt ttgggattca aaccaacagt ctgtcagtgt acagcagcag tactctcctg
6661 cacagtctca agcaaccata tattatcaag gacagacatg tccaacagtc tatggtgtga
6721 catcacctta ttcacagaca actccaccaa ttgtacagag ttatgcccag ccaagtcttc
6781 agtatatcca ggggcaacag attttcacag ctcatccaca aggagtggtg gtacagccag
6841 ccgcagcagt gactacaata gttgcaccag ggcagcctca gcccttgcag ccatctgaaa
6901 tggttgtgac aaataatctc ttggatctgc cgcccccctc tcctcccaaa ccaaaaacca
6961 ttgtcttacc tcccaactgg aagacagctc gagatccaga agggaagatt tattactacc
7021 atgtgatcac aaggcagact cagtgggatc ctcctacttg ggaaagccca ggagatgatg
7081 ccagccttga gcatgaagct gagatggacc tgggaactcc aacatatgat gaaaacccca
7141 tgaaggcctc gaaaaagccc aagacagcag aagcagacac ctccagtgaa ctagcaaaga
7201 aaagcaaaga agtattcaga aaagagatgt cccagttcat cgtccagtgc ctgaaccctt
7261 accggaaacc tgactgcaaa gtgggaagaa ttaccacaac tgaagacttt aaacatctgg
7321 ctcgcaagct gactcacggt gttatgaata aggagctgaa gtactgtaag aatcctgagg
7381 acctggagtg caatgagaat gtgaaacaca aaaccaagga gtacattaag aagtacatgc
7441 agaagtttgg ggctgtttac aaacccaaag aggacactga attagagtga ctgttgggcc
7501 agggtgggag gatgggtggt caggtaagac agactctagg gagaggaaat cctgtgggcc
7561 tttctgtccc acccctgtca gcactgtgct actgatgata catcaccctg gggaattcaa
7621 ccctgcagtc gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat
7681 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
7741 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
7801 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
7861 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta
7921 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
7981 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
8041 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
8101 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
8161 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
8221 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
8281 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
8341 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
8401 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
8461 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
8521 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
8581 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
8641 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
8701 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
8761 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
8821 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
8881 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
8941 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
9001 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
9061 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
9121 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
9181 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
9241 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
9301 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
9361 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
9421 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
9481 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
9541 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
9601 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
9661 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
9721 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
9781 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
9841 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
9901 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
9961 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
10021 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
10081 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
10141 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
10201 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
10261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
10321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
10381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
10441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
10501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
10561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
10621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
10681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
10741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
10801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
10861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
10921 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
10981 cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat
//
