| 出品公司: | CAMBIA |
|---|---|
| 载体名称: | pCAMBIA0305.2 |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lac |
| 载体大小: | 9737 bp |
| 5' 测序引物及序列: | M13 Reverse:CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | 分泌型GUS、6xHis |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | -- |
| 克隆菌株: | HB101等菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pCAMBIA0305.2载体质粒基本信息
pCAMBIA0305.2质粒图谱载体图谱和pCAMBIA0305.2载体序列质粒序列多克隆位点信息
pCAMBIA0305.2质粒载体简介
pCAMBIA0305.2质粒序列载体序列
LOCUS pCAMBIA0305.2 9737 bp ds-DNA circular SYN 25-JUL-2016
DEFINITION Agrobacterium binary vector for plant transformation, with
kanamycin-resistance and secreted GUSPlus(TM) genes.
SOURCE http://www.biofeng.com/
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9737)
AUTHORS Cambia
TITLE Direct Submission
JOURNAL Exported Monday, July 25, 2016 from SnapGene Viewer 3.1.4 to Vector
NTI(R) format
http://www.snapgene.com
COMMENT The GenBank record was corrected by inserting a G at position 4815.
COMMENT VNTDATE|658800000|
COMMENT VNTDBDATE|776304000|
COMMENT VNTNAME|pCAMBIA0305.2|
COMMENT VNTAUTHORNAME|Cambia|
FEATURES Location/Qualifiers
CDS 2..82
/codon_start=1
/product="signal sequence from Oryza sativa glycine-rich
cell wall protein"
/label=GRP signal sequence
CDS join(83..91,282..2099)
/codon_start=1
/product="beta-glucuronidase"
/label=GUSPlus(TM)
/note="codon-optimized Staphylococcus gusA gene with a
catalase intron to ensure expression in plants but not
bacteria"
intron 92..281
/label=cat1 intron
/note="castor bean catalase intron, modified"
CDS 2106..2123
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
terminator 2155..2407
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 2429..2453
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 3753..4382
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/label=pVS1 StaA
CDS 4811..5884
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 5950..6144
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 6488..6628
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(6814..7402)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7489..8283)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
misc_feature 8708..8732
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
promoter 8836..8866
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8874..8890
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8898..8914
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 8910..9143
/codon_start=1
/gene="lacZ (truncated)"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
misc_feature 8924..8980
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(8984..9000)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 9377..9722
/label=35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus.CaMV 35S promoter"
ORIGIN
1 catggctact actaagcatt tggctcttgc catccttgtc ctccttagca ttggtatgac
61 caccagtgca agaaccctcc tagatctgag ggtaaatttc tagtttttct ccttcatttt
121 cttggttagg acccttttct ctttttattt ttttgagctt tgatctttct ttaaactgat
181 ctatttttta attgattggt tatggtgtaa atattacata gctttaactg ataatctgat
241 tactttattt cgtgtgtcta tgatgatgat gatagttaca gaaccgacga actagtctgt
301 acccgatcaa caccgagacc cgtggcgtct tcgacctcaa tggcgtctgg aacttcaagc
361 tggactacgg gaaaggactg gaagagaagt ggtacgaaag caagctgacc gacactatta
421 gtatggccgt cccaagcagt tacaatgaca ttggcgtgac caaggaaatc cgcaaccata
481 tcggatatgt ctggtacgaa cgtgagttca cggtgccggc ctatctgaag gatcagcgta
541 tcgtgctccg cttcggctct gcaactcaca aagcaattgt ctatgtcaat ggtgagctgg
601 tcgtggagca caagggcgga ttcctgccat tcgaagcgga aatcaacaac tcgctgcgtg
661 atggcatgaa tcgcgtcacc gtcgccgtgg acaacatcct cgacgatagc accctcccgg
721 tggggctgta cagcgagcgc cacgaagagg gcctcggaaa agtcattcgt aacaagccga
781 acttcgactt cttcaactat gcaggcctgc accgtccggt gaaaatctac acgaccccgt
841 ttacgtacgt cgaggacatc tcggttgtga ccgacttcaa tggcccaacc gggactgtga
901 cctatacggt ggactttcaa ggcaaagccg agaccgtgaa agtgtcggtc gtggatgagg
961 aaggcaaagt ggtcgcaagc accgagggcc tgagcggtaa cgtggagatt ccgaatgtca
1021 tcctctggga accactgaac acgtatctct accagatcaa agtggaactg gtgaacgacg
1081 gactgaccat cgatgtctat gaagagccgt tcggcgtgcg gaccgtggaa gtcaacgacg
1141 gcaagttcct catcaacaac aaaccgttct acttcaaggg ctttggcaaa catgaggaca
1201 ctcctatcaa cggccgtggc tttaacgaag cgagcaatgt gatggatttc aatatcctca
1261 aatggatcgg cgccaacagc ttccggaccg cacactatcc gtactctgaa gagttgatgc
1321 gtcttgcgga tcgcgagggt ctggtcgtga tcgacgagac tccggcagtt ggcgtgcacc
1381 tcaacttcat ggccaccacg ggactcggcg aaggcagcga gcgcgtcagt acctgggaga
1441 agattcggac gtttgagcac catcaagacg ttctccgtga actggtgtct cgtgacaaga
1501 accatccaag cgtcgtgatg tggagcatcg ccaacgaggc ggcgactgag gaagagggcg
1561 cgtacgagta cttcaagccg ttggtggagc tgaccaagga actcgaccca cagaagcgtc
1621 cggtcacgat cgtgctgttt gtgatggcta ccccggagac ggacaaagtc gccgaactga
1681 ttgacgtcat cgcgctcaat cgctataacg gatggtactt cgatggcggt gatctcgaag
1741 cggccaaagt ccatctccgc caggaatttc acgcgtggaa caagcgttgc ccaggaaagc
1801 cgatcatgat cactgagtac ggcgcagaca ccgttgcggg ctttcacgac attgatccag
1861 tgatgttcac cgaggaatat caagtcgagt actaccaggc gaaccacgtc gtgttcgatg
1921 agtttgagaa cttcgtgggt gagcaagcgt ggaacttcgc ggacttcgcg acctctcagg
1981 gcgtgatgcg cgtccaagga aacaagaagg gcgtgttcac tcgtgaccgc aagccgaagc
2041 tcgccgcgca cgtctttcgc gagcgctgga ccaacattcc agatttcggc tacaagaacg
2101 ctagccatca ccatcaccat cacgtgtgaa ttggtgacca gctcgaattt ccccgatcgt
2161 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
2221 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
2281 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
2341 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
2401 ctagatcggg aattaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
2461 aaaagagcgt ttattagaat aacggatatt taaaagggcg tgaaaaggtt tatccgttcg
2521 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
2581 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa
2641 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
2701 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
2761 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
2821 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
2881 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
2941 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
3001 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
3061 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
3121 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
3181 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
3241 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
3301 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
3361 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
3421 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
3481 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
3541 ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
3601 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
3661 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
3721 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
3781 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
3841 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
3901 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
3961 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
4021 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
4081 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
4141 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
4201 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
4261 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
4321 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
4381 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
4441 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
4501 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
4561 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
4621 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
4681 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
4741 caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg
4801 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
4861 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
4921 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
4981 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
5041 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
5101 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
5161 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
5221 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
5281 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
5341 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
5401 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
5461 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
5521 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
5581 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
5641 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
5701 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
5761 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
5821 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
5881 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
5941 tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
6001 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
6061 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
6121 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa
6181 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
6241 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
6301 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
6361 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
6421 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
6481 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
6541 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
6601 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
6661 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
6721 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
6781 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
6841 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
6901 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
6961 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
7021 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
7081 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
7141 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
7201 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
7261 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
7321 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
7381 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
7441 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc
7501 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa
7561 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc
7621 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt
7681 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc
7741 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc
7801 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc
7861 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa
7921 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata
7981 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg
8041 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc
8101 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata
8161 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc
8221 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc
8281 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat
8341 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca
8401 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg
8461 aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
8521 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
8581 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
8641 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
8701 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca
8761 cattgcggac gtttttaatg tactgaatta acgccgaatt aatgtgagtt agctcactca
8821 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
8881 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattcg agctcggtac
8941 ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggcactggcc gtcgttttac
9001 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
9061 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
9121 gcagcctgaa tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc
9181 ttcagtttag cttcatggag tcaaagattc aaatagagga cctaacagaa ctcgccgtaa
9241 agactggcga acagttcata cagagtctct tacgactcaa tgacaagaag aaaatcttcg
9301 tcaacatggt ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag
9361 aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat
9421 tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct
9481 acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg
9541 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
9601 cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat
9661 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagaa
9721 cacgggggac tcttgac
//
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