| 质粒名称: | Desmoplakin II acceptor only control (F40-based tension sensor) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 118719 |
| 存储实验室: | Carsten Grashoff |
|
载体骨架基本信息 |
|
| 载体名称: | pLPCX |
| 载体出品公司: | Clontech |
| 空载体大小: | 6265 bp |
| 完整质粒大小: | 14585 bp |
| 载体类型: | 哺乳细胞表达、Retroviral |
| 载体抗性: | Puromycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | DPII-int (dYF40C) / Desmoplakin II acceptor only control for F40-based tension sensor / human Desmoplakin II-[YPet(short)(Y67G)-F40-mCherry] (internal-1353) |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 8352 bp |
| 基因突变: | inserted F40-based tension sensor module after aa1353: Y67G in YPet(short) mutation to disrupt chromophore formation |
| GenBank编号 | NM_001008844.1 |
| 启动子: | CMV |
| 标签/融合蛋白: | YPet(short)(Y67G)-F40-mCherry |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | AGCTcGTTTAGTGAACCGTCAGATC |
| 3’测序引物 | ACCTACAGGTGGGGTCTTTCATTCCC |
|
文献引用方法 |
|
| 材料和方法部分: |
Desmoplakin II acceptor only control (F40-based tension sensor) was a gift from Carsten Grashoff (Addgene plasmid # 118719 ; http://n2t.net/addgene:118719 ; RRID:Addgene_118719)
|
| 参考文献部分: | Mechanical loading of desmosomes depends on the magnitude and orientation of external stress. Price AJ, Cost AL, Ungewiss H, Waschke J, Dunn AR, Grashoff C. Nat Commun. 2018 Dec 11;9(1):5284. doi: 10.1038/s41467-018-07523-0. 10.1038/s41467-018-07523-0 PubMed 30538252 |
Desmoplakin II acceptor only control (F40-based tension sens
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS Desmoplakin II acceptor only control (F40-based tension sensor) 14585 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION The acceptor (mCherry) only control for the F40-based human
desmoplakin II tension sensor can be co-expressed with the donor
(YPet(short)) only control to determine intermolecular FRET..
ACCESSION .
VERSION .
KEYWORDS Desmoplakin II acceptor only control (F40-based tension sensor)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14585)
AUTHORS Price AJ, Cost AL, Ungewiss H, Waschke J, Dunn AR, Grashoff C
TITLE Mechanical loading of desmosomes depends on the magnitude and
orientation of external stress.
JOURNAL Nat Commun. 2018 Dec 11;9(1):5284. doi: 10.1038/s41467-018-07523-0.
PUBMED 30538252
REFERENCE 2 (bases 1 to 14585)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..14585
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 414..589
/label=5' LTR (truncated)
/note="truncated long terminal repeat from Moloney murine
sarcoma virus"
misc_feature 652..851
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1052..1468
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1398..1420
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1436..1452
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS 1565..2164
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1565..1584)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2061..2081
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
enhancer 2339..2642
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2643..2846
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2797..2817
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind complement(7292..7311)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
CDS 7802..8509
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(7947..7965)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 8215..8234
/label=mCherry-F
/note="mCherry, forward primer"
LTR 11324..11917
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(12020..12042)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
protein_bind complement(12170..12203)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
protein_bind complement(12263..12296)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
primer_bind 12316..12335
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(12416..12433)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(12587..13175)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(12667..12686)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(13346..14206)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 13969..13988
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(14207..14311)
/gene="bla"
/label=AmpR promoter
primer_bind 14379..14397
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
1441 tccagccctc actccttctc taggcgccgg aatgatcccc cgggctgcag gtcggccgcc
1501 acgaccggtg ccgccaccat cccctgaccc acgcccctga cccctcacaa ggagacgacc
1561 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
1621 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
1681 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
1741 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
1801 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
1861 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
1921 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
1981 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
2041 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
2101 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
2161 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc
2221 cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag gcccaccgac
2281 tctagtaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
2341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
2401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
2461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
2521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
2581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
2641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
2701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
2761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
2821 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
2881 ggactcagat ctgggcccat aaagcttata cgaattcacc atgagctgca acggaggctc
2941 ccacccgcgg atcaacactc tgggccgcat gatccgcgcc gagtctggcc cggacctgcg
3001 ctacgaggtg accagcggcg gcgggggcac cagcaggatg tactattctc ggcgcggcgt
3061 gatcaccgac cagaactcgg acggctactg tcaaaccggc acgatgtcca ggcaccagaa
3121 ccagaacacc atccaggagc tgctgcagaa ctgctccgac tgcttgatgc gagcagagct
3181 catcgtgcag cctgaattga agtatggaga tggaatacaa ctgactcgga gtcgagaatt
3241 ggatgagtgt tttgcccagg ccaatgacca aatggaaatc ctcgacagct tgatcagaga
3301 gatgcggcag atgggccagc cctgtgatgc ttaccagaaa aggcttcttc agctccaaga
3361 gcaaatgcga gccctttata aagccatcag tgtcccacga gtccgcaggg ccagctccaa
3421 gggtggtgga ggctacactt gtcagagtgg ctctggctgg gatgagttca ccaaacatgt
3481 caccagtgaa tgtttggggt ggatgaggca gcaaagggcg gagatggaca tggtggcctg
3541 gggtgtggac ctggcctcag tggagcagca cattaacagc caccggggca tccacaactc
3601 catcggcgac tatcgctggc agctggacaa aatcaaagcc gacctgcgcg agaaatctgc
3661 gatctaccag ttggaggagg agtatgaaaa cctgctgaaa gcgtcctttg agaggatgga
3721 tcacctgcga cagctgcaga acatcattca ggccacgtcc agggagatca tgtggatcaa
3781 tgactgcgag gaggaggagc tgctgtacga ctggagcgac aagaacacca acatcgctca
3841 gaaacaggag gccttctcca tacgcatgag tcaactggaa gttaaagaaa aagagctcaa
3901 taagctgaaa caagaaagtg accaacttgt cctcaatcag catccagctt cagacaaaat
3961 tgaggcctat atggacactc tgcagacgca gtggagttgg attcttcaga tcaccaagtg
4021 cattgatgtt catctgaaag aaaatgctgc ctactttcag ttttttgaag aggcgcagtc
4081 tactgaagca tacctgaagg ggctccagga ctccatcagg aagaagtacc cctgcgacaa
4141 gaacatgccc ctgcagcacc tgctggaaca gatcaaggag ctggagaaag aacgagagaa
4201 aatccttgaa tacaagcgtc aggtgcagaa cttggtaaac aagtctaaga agattgtaca
4261 gctgaagcct cgtaacccag actacagaag caataaaccc attattctca gagctctctg
4321 tgactacaaa caagatcaga aaatcgtgca taagggggat gagtgtatcc tgaaggacaa
4381 caacgagcgc agcaagtggt acgtgacggg cccgggaggc gttgacatgc ttgttccctc
4441 tgtggggctg atcatccctc ctccgaaccc actggccgtg gacctctctt gcaagattga
4501 gcagtactac gaagccatct tggctctgtg gaaccagctc tacatcaaca tgaagagcct
4561 ggtgtcctgg cactactgca tgattgacat agagaagatc agggccatga caatcgccaa
4621 gctgaaaaca atgcggcagg aagattacat gaagacgata gccgaccttg agttacatta
4681 ccaagagttc atcagaaata gccaaggctc agagatgttt ggagatgatg acaagcggaa
4741 aatacagtct cagttcaccg atgcccagaa gcattaccag accctggtca ttcagctccc
4801 tggctatccc cagcaccaga cagtgaccac aactgaaatc actcatcatg gaacctgcca
4861 agatgtcaac cataataaag taattgaaac caacagagaa aatgacaagc aagaaacatg
4921 gatgctgatg gagctgcaga agattcgcag gcagatagag cactgcgagg gcaggatgac
4981 tctcaaaaac ctccctctag cagaccaggg gtcttctcac cacatcacag tgaaaattaa
5041 cgagcttaag agtgtgcaga atgattcaca agcaattgct gaggttctca accagcttaa
5101 agatatgctt gccaacttca gaggttctga aaagtactgc tatttacaga atgaagtatt
5161 tggactattt cagaaactgg aaaatatcaa tggtgttaca gatggctact taaatagctt
5221 atgcacagta agggcactgc tccaggctat tctccaaaca gaagacatgt taaaggttta
5281 tgaagccagg ctcactgagg aggaaactgt ctgcctggac ctggataaag tggaagctta
5341 ccgctgtgga ctgaagaaaa taaaaaatga cttgaacttg aagaagtcgt tgttggccac
5401 tatgaagaca gaactacaga aagcccagca gatccactct cagacttcac agcagtatcc
5461 actttatgat ctggacttgg gcaagttcgg tgaaaaagtc acacagctga cagaccgctg
5521 gcaaaggata gataaacaga tcgactttag attatgggac ctggagaaac aaatcaagca
5581 attgaggaat tatcgtgata actatcaggc tttctgcaag tggctctatg atgctaaacg
5641 ccgccaggat tccttagaat ccatgaaatt tggagattcc aacacagtca tgcggttttt
5701 gaatgagcag aagaacttgc acagtgaaat atctggcaaa cgagacaaat cagaggaagt
5761 acaaaaaatt gctgaacttt gcgccaattc aattaaggat tatgagctcc agctggcctc
5821 atacacctca ggactggaaa ctctgctgaa catacctatc aagaggacca tgattcagtc
5881 cccttctggg gtgattctgc aagaggctgc agatgttcat gctcggtaca ttgaactact
5941 tacaagatct ggagactatt acaggttctt aagtgagatg ctgaagagtt tggaagatct
6001 gaagctgaaa aataccaaga tcgaagtttt ggaagaggag ctcagactgg cccgagatgc
6061 caactcggaa aactgtaata agaacaaatt cctggatcag aacctgcaga aataccaggc
6121 agagtgttcc cagttcaaag cgaagcttgc gagcctggag gagctgaaga gacaggctga
6181 gctggatggg aagtcggcta agcaaaatct agacaagtgc tacggccaaa taaaagaact
6241 caatgagaag atcacccgac tgacttatga gattgaagat gaaaagagaa gaagaaaatc
6301 tgtggaagac agatttgacc aacagaagaa tgactatgac caactgcaga aagcaaggca
6361 atgtgaaaag gagaaccttg gttggcagaa attagagtct gagaaagcca tcaaggagaa
6421 ggagtacgag attgaaaggt tgagggttct actgcaggaa gaaggcaccc ggaagagaga
6481 atatgaaaat gagctggcaa aggcatctaa taggattcag gaatcaaaga atcagtgtac
6541 tcaggtggta caggaaagag agagccttct ggtgaaaatc aaagtcctgg agcaagacaa
6601 ggcaaggctg cagaggctgg aggatgagct gaatcgtgca aaatcaactc tagaggcaga
6661 aaccagggtg aaacagcgcc tggagtgtga gaaacagcaa attcagaatg acctgaatca
6721 gtggaagact caatattccc gcaaggagga ggctattagg aagatagaat cggaaagaga
6781 aaagagtgag agagagaaga acagtcttag gagtgagatc gaaagactcc aagcagagat
6841 caagagaatt gaagagaggt gcaggcgtaa gctggaggat tctaccaggg agacacagtc
6901 acagttagaa acagaacgct cccgatatca gagggagatt gataaactca gacagcgccc
6961 atatgggtcc catcgagagg tcgagatggt gagcaaaggc gaagagctgt tcaccggcgt
7021 ggtgcccatc ctggtggagc tggacggcga cgtgaacggc cacaagttca gcgtgagcgg
7081 cgagggcgag ggcgacgcca cctacggcaa gctgaccctg aagctgctgt gcaccaccgg
7141 caagctgccc gtgccctggc ccaccctggt gaccaccctg ggcggcggcg tgcagtgctt
7201 cgcccggtac cccgaccaca tgaagcagca cgacttcttc aagagcgcca tgcccgaggg
7261 ctacgtgcag gagcggacca tcttcttcaa ggacgacggc aactacaaga cccgggccga
7321 ggtgaagttc gagggcgaca ccctggtgaa ccggatcgag ctgaagggca tcgacttcaa
7381 ggaggacggc aacatcctgg gccacaagct ggagtacaac tacaacagcc acaacgtgta
7441 catcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc ggcacaacat
7501 cgaggacggc ggcgtgcagc tggccgacca ctaccagcag aacaccccca tcggcgacgg
7561 ccccgtgctg ctgcccgaca accactacct gagctaccag agcgccctgt tcaaggaccc
7621 caacgagaag cgggaccaca tggtgctgct ggagttcctg accgccgccg gatccgggcc
7681 aggtggagca gggccaggtg gtgcagggcc aggtggtgca gggccaggtg gtgcagggcc
7741 cggtggtgca ggtccaggtg gtgcaggtcc aggtggtgct ggtccaggtg gtgctggatc
7801 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
7861 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
7921 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
7981 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
8041 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
8101 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
8161 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
8221 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
8281 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
8341 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
8401 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
8461 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg cggccgctac
8521 ccagactgag tgtgagtgga ccgttgacac ctccaagctg gtgtttgatg ggctgaggaa
8581 gaaggtgaca gcaatgcagc tctatgagtg tcagctgatc gacaaaacaa ccttggacaa
8641 actattgaag gggaagaagt cagtggaaga agttgcttct gaaatccagc cattccttcg
8701 gggtgcagga tctatcgctg gagcatctgc ttctcctaag gaaaaatact ctttggtaga
8761 ggccaagaga aagaaattaa tcagcccaga atccacagtc atgcttctgg aggcccaggc
8821 agctacaggt ggtataattg atccccatcg gaatgagaag ctgactgtgg acagtgccat
8881 agctcgggac ctcattgact tcgatgaccg tcagcagata tatgcagcag aaaaagctat
8941 cactggtttt gatgatccat tttcaggcaa gacagtatct gtttcagaag ccatcaagaa
9001 aaatttgatt gatagagaaa ccggaatgcg cctgctggaa gcccagattg cttcaggggg
9061 tgtagtagac cctgtgaaca gtgtcttttt gccaaaagat gtcgccttgg cccgggggct
9121 gattgataga gatttgtatc gttccctgaa tgatccccga gatagtcaga aaaactttgt
9181 ggatccagtc accaaaaaga aggtcagtta cgtgcagctg aaggaacggt gcagaatcga
9241 accacatact ggtctgctct tgctttcagt acagaagaga agcatgtcct tccaaggaat
9301 cagacaacct gtgaccgtca ctgagctagt agattctggt atattgagac cgtccactgt
9361 caatgaactg gaatctggtc agatttctta tgacgaggtt ggtgagagaa ttaaggactt
9421 cctccagggt tcaagctgca tagcaggcat atacaatgag accacaaaac agaagcttgg
9481 catttatgag gccatgaaaa ttggcttagt ccgacctggt actgctctgg agttgctgga
9541 agcccaagca gctactggct ttatagtgga tcctgttagc aacttgaggt taccagtgga
9601 ggaagcctac aagagaggtc tggtgggcat tgagttcaaa gagaagctcc tgtctgcaga
9661 acgagctgtc actgggtata atgatcctga aacaggaaac atcatctctt tgttccaagc
9721 catgaataag gaactcatcg aaaagggcca cggtattcgc ttattagaag cacagatcgc
9781 aaccgggggg atcattgacc caaaggagag ccatcgttta ccagttgaca tagcatataa
9841 gaggggctat ttcaatgagg aactcagtga gattctctca gatccaagtg atgataccaa
9901 aggatttttt gaccccaaca ctgaagaaaa tcttacctat ctgcaactaa aagaaagatg
9961 cattaaggat gaggaaacag ggctctgtct tctgcctctg aaagaaaaga agaaacaggt
10021 gcagacatca caaaagaata ccctcaggaa gcgtagagtg gtcatagttg acccagaaac
10081 caataaagaa atgtctgttc aggaggccta caagaagggc ctaattgatt atgaaacctt
10141 caaagaactg tgtgagcagg aatgtgaatg ggaagaaata accatcacgg gatcagatgg
10201 ctccaccagg gtggtcctgg tagatagaaa gacaggcagt cagtatgata ttcaagatgc
10261 tattgacaag ggccttgttg acaggaagtt ctttgatcag taccgatccg gcagcctcag
10321 cctcactcaa tttgctgaca tgatctcctt gaaaaatggt gtcggcacca gcagcagcat
10381 gggcagtggt gtcagcgatg atgtttttag cagctcccga catgaatcag taagtaagat
10441 ttccaccata tccagcgtca ggaatttaac cataaggagc agctcttttt cagacaccct
10501 ggaagaatcg agccccattg cagccatctt tgacacagaa aacctggaga aaatctccat
10561 tacagaaggt atagagcggg gcatcgttga cagcatcacg ggtcagaggc ttctggaggc
10621 tcaggcctgc acaggtggca tcatccaccc aaccacgggc cagaagctgt cacttcagga
10681 cgcagtctcc cagggtgtga ttgaccaaga catggccacc aggctgaagc ctgctcagaa
10741 agccttcata ggcttcgagg gtgtgaaggg aaagaagaag atgtcagcag cagaggcagt
10801 gaaagaaaaa tggctcccgt atgaggctgg ccagcgcttc ctggagttcc agtacctcac
10861 gggaggtctt gttgacccgg aagtgcatgg gaggataagc accgaagaag ccatccggaa
10921 ggggttcata gatggccgcg ccgcacagag gctgcaagac accagcagct atgccaaaat
10981 cctgacctgc cccaaaacca aattaaaaat atcctataag gatgccataa atcgctccat
11041 ggtagaagat atcactgggc tgcgccttct ggaagccgcc tccgtgtcgt ccaagggctt
11101 acccagccct tacaacatgt cttcggctcc ggggtcccgc tccggctccc gctcgggatc
11161 tcgctccgga tctcgctccg ggtcccgcag tgggtcccgg agaggaagct ttgacgccac
11221 aggcaattct tcctactctt attcctactc atttagcagt agttctattg ggcactaaat
11281 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
11341 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac
11401 tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa
11461 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag
11521 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
11581 gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt
11641 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
11701 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
11761 ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa
11821 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
11881 tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc gggatcggga
11941 gacccctgcc cagggaccac cgacccacca ccgggaggta agctggctgc ctcgcgcgtt
12001 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
12061 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
12121 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtactga taacttcgta
12181 taatgtatgc tatacgaagt tattaggtct gaagaggagt ttacgtccag ccaagctagg
12241 gccgcgatcc ggaaccctta atataacttc gtataatgta tgctatacga agttatcagt
12301 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
12361 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
12421 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
12481 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
12541 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
12601 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
12661 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
12721 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
12781 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
12841 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
12901 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
12961 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
13021 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
13081 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
13141 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
13201 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
13261 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
13321 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
13381 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
13441 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
13501 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
13561 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
13621 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
13681 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
13741 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
13801 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
13861 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
13921 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
13981 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
14041 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
14101 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
14161 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
14221 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
14281 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
14341 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
14401 ttcgtcttca agaattgcta gcaattgcta gcaattgcta gcaattcata ccagatcacc
14461 gaaaactgtc ctccaaatgt gtccccctca cactcccaaa ttcgcgggct tctgcctctt
14521 agaccactct accctattcc ccacactcac cggagccaaa gccgcggccc ttccgtttct
14581 ttgct
//
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