| 出品公司: | Clontech |
|---|---|
| 载体名称: | pZsYellow-N1,pZsYellowN1 |
| 质粒类型: | 荧光蛋白报告载体，哺乳细胞表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 启动子: | CMV |
| 载体大小: | 4708 dp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 卡那青霉素 |
| 筛选标记: | 新霉素 |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
| pRFP-N3 |
LOCUS pZsGreen1-N1 4708 bp ds-DNA circular SYN 19-OCT-2017
DEFINITION Vector for fusing ZsGreen1 to the C-terminus of a partner protein.
ACCESSION .
VERSION .
KEYWORDS pZsGreen1-N1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4708)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Saturday, November 16, 2019 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4708
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 591..671
/note="MCS"
/note="multiple cloning site of fluorescent protein
plasmids"
CDS 679..1374
/codon_start=1
/product="Zoanthus green fluorescent protein"
/note="ZsGreen1"
/note="mammalian codon-optimized"
/translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
AIASGSALP"
polyA_signal 1496..1617
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1624..2079)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2106..2210
/gene="bla"
/note="AmpR promoter"
promoter 2212..2569
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2420..2555
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2604..3398
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 3630..3677
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 4006..4594
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
661 gatccaccgg tcgccaccat ggcccagtcc aagcacggcc tgaccaagga gatgaccatg
721 aagtaccgca tggagggctg cgtggacggc cacaagttcg tgatcaccgg cgagggcatc
781 ggctacccct tcaagggcaa gcaggccatc aacctgtgcg tggtggaggg cggccccttg
841 cccttcgccg aggacatctt gtccgccgcc ttcatgtacg gcaaccgcgt gttcaccgag
901 tacccccagg acatcgtcga ctacttcaag aactcctgcc ccgccggcta cacctgggac
961 cgctccttcc tgttcgagga cggcgccgtg tgcatctgca acgccgacat caccgtgagc
1021 gtggaggaga actgcatgta ccacgagtcc aagttctacg gcgtgaactt ccccgccgac
1081 ggccccgtga tgaagaagat gaccgacaac tgggagccct cctgcgagaa gatcatcccc
1141 gtgcccaagc agggcatctt gaagggcgac gtgagcatgt acctgctgct gaaggacggt
1201 ggccgcttgc gctgccagtt cgacaccgtg tacaaggcca agtccgtgcc ccgcaagatg
1261 cccgactggc acttcatcca gcacaagctg acccgcgagg accgcagcga cgccaagaac
1321 cagaagtggc acctgaccga gcacgccatc gcctccggct ccgccttgcc ctgagcggcc
1381 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
1441 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
1501 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
1561 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg
1621 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
1681 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
1741 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
1801 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
1861 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
1921 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
1981 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
2041 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
2101 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
2161 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
2221 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
2281 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
2341 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
2401 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
2461 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
2521 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
2581 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
2641 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
2701 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
2761 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
2821 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
2881 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
2941 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
3001 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
3061 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
3121 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
3181 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
3241 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
3301 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
3361 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
3421 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
3481 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
3541 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
3601 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
3661 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
3721 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
3781 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
3841 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
3901 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
3961 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
4021 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
4081 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
4141 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
4201 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
4261 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
4321 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
4381 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
4441 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
4501 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
4561 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
4621 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
4681 tgtggataac cgtattaccg ccatgcat
//
