出品公司: | Microbix Biosystems |
---|---|
载体名称: | pDC316 |
质粒类型: | 腺病毒表达载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | MCMV |
克隆方法: | -- |
载体大小: | 3913 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | M13-R: CAGGAAACAGCTATGAC |
载体标签: | -- |
载体抗性: | 氨苄青霉素(Ampicillin) |
筛选标记: | -- |
备注: | 克隆宿主菌DB3.1 |
产品目录号: | -- |
稳定性: | -- |
组成型: | 组成型 |
病毒/非病毒: | 腺病毒 |
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pDC316载体质粒基本信息
pDC316质粒图谱载体图谱和pDC316载体序列质粒序列多克隆位点信息
pDC316质粒载体简介
pDC316质粒序列载体序列
LOCUS pDC316 3913 bp dsDNA Circular DEFINITION Puc based lox HCMV shuttle plasmid for Cre-based Ad vector rescue Cummings & Graham, unpublished COMMENT This file is best viewed with a free Vector NTI Viewer: COMMENT http://www.informaxinc.com/view/viewer.html COMMENT VNTREPLTYPE|Plasmid| COMMENT VNTEXTCHREPL|Bacteria| FEATURES Location/Qualifiers promoter 469..991 /note="MCMV promoter - Short" /label=MCMV polyA_signal 1029..1188 /label=SV40\PolyA misc_feature 1190..1223 /label=loxP\ rep_origin 1603..1903 /label=ori CDS complement(2353..3169) /label=amp misc_feature 3454..453 /label=Ad CDS 3823..1 /label=ITR CDS complement(2..102) /label=ITR ORIGIN 1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg 361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc 421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gatatactga gtcattaggg 481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc 541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa 601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa 661 taggggtgag tcattgggtt tttccagcca tttaattaaa acgccatgta ctttcccacc 721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat 781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg 841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cactcattct 901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca 961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattccc cgggagatct aagcttgagc 1021 tcgtcgactt cgagcaactt gtttattgca gcttataatg gttacaaata aagcaatagc 1081 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 1141 ctcatcaatg tatcttatca tgtctggatc gtctagcatc gaagatccaa taacttcgta 1201 tagcatacat tatacgaagt tataagtagc ttggcgtaat catggtcata gctgtttcct 1261 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 1321 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 1381 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 1441 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 1501 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 1561 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 1621 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 1681 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 1741 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 1801 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat 1861 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 1921 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 1981 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 2041 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 2101 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 2161 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 2221 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 2281 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 2341 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 2401 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 2461 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 2521 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 2581 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 2641 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 2701 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 2761 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 2821 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 2881 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 2941 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 3001 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa 3061 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 3121 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 3181 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 3241 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 3301 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 3361 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 3421 atgacattaa cctataaaaa taggcgtatc actctaggca aaatagcacc ctcccgctcc 3481 agaacaacat acagcgcttc acagcggcag cctaacagtc agccttacca gtaaaaaaga 3541 aaacctatta aaaaaacacc actcgacacg gcaccagctc aatcagtcac agtgtaaaaa 3601 agggccaagt gcagagcgag tatatatagg actaaaaaat gacgtaacgg ttaaagtcca 3661 caaaaaacac ccagaaaacc gcacgcgaac ctacgcccag aaacgaaagc caaaaaaccc 3721 acaacttcct caaatcgtca cttccgtttt cccacgttac gtaacttccc attttaagaa 3781 aactacaatt cccaacacat acaagttact ccgccctaaa acctacgtca cccgccccgt 3841 tcccacgccc cgcgccacgt cacaaactcc accccctcat tatcatattg gcttcaatcc 3901 aaaataaggt ata //
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pDC316质粒载体应用举例
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