| 出品公司: | Microbix Biosystems |
|---|---|
| 载体名称: | pDC316 |
| 质粒类型: | 腺病毒表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | MCMV |
| 克隆方法: | -- |
| 载体大小: | 3913 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | M13-R: CAGGAAACAGCTATGAC |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素（Ampicillin） |
| 筛选标记: | -- |
| 备注: | 克隆宿主菌DB3.1 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | 组成型 |
| 病毒/非病毒: | 腺病毒 |
买家导航
pDC316载体质粒基本信息
pDC316质粒图谱载体图谱和pDC316载体序列质粒序列多克隆位点信息
pDC316质粒载体简介
pDC316质粒序列载体序列
LOCUS pDC316 3913 bp dsDNA Circular
DEFINITION Puc based lox HCMV shuttle plasmid for Cre-based Ad vector rescue Cummings & Graham, unpublished
COMMENT This file is best viewed with a free Vector NTI Viewer:
COMMENT http://www.informaxinc.com/view/viewer.html
COMMENT VNTREPLTYPE|Plasmid|
COMMENT VNTEXTCHREPL|Bacteria|
FEATURES Location/Qualifiers
promoter 469..991
/note="MCMV promoter - Short"
/label=MCMV
polyA_signal 1029..1188
/label=SV40\PolyA
misc_feature 1190..1223
/label=loxP\
rep_origin 1603..1903
/label=ori
CDS complement(2353..3169)
/label=amp
misc_feature 3454..453
/label=Ad
CDS 3823..1
/label=ITR
CDS complement(2..102)
/label=ITR
ORIGIN
1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gatatactga gtcattaggg
481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc
541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa
601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa
661 taggggtgag tcattgggtt tttccagcca tttaattaaa acgccatgta ctttcccacc
721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat
781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg
841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cactcattct
901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca
961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattccc cgggagatct aagcttgagc
1021 tcgtcgactt cgagcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
1081 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
1141 ctcatcaatg tatcttatca tgtctggatc gtctagcatc gaagatccaa taacttcgta
1201 tagcatacat tatacgaagt tataagtagc ttggcgtaat catggtcata gctgtttcct
1261 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
1321 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
1381 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
1441 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
1501 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
1561 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
1621 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
1681 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
1741 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
1801 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
1861 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
1921 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
1981 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
2041 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
2101 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
2161 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
2221 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
2281 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
2341 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
2401 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
2461 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
2521 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
2581 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
2641 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
2701 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
2761 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
2821 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
2881 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
2941 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
3001 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
3061 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
3121 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
3181 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
3241 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
3301 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
3361 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
3421 atgacattaa cctataaaaa taggcgtatc actctaggca aaatagcacc ctcccgctcc
3481 agaacaacat acagcgcttc acagcggcag cctaacagtc agccttacca gtaaaaaaga
3541 aaacctatta aaaaaacacc actcgacacg gcaccagctc aatcagtcac agtgtaaaaa
3601 agggccaagt gcagagcgag tatatatagg actaaaaaat gacgtaacgg ttaaagtcca
3661 caaaaaacac ccagaaaacc gcacgcgaac ctacgcccag aaacgaaagc caaaaaaccc
3721 acaacttcct caaatcgtca cttccgtttt cccacgttac gtaacttccc attttaagaa
3781 aactacaatt cccaacacat acaagttact ccgccctaaa acctacgtca cccgccccgt
3841 tcccacgccc cgcgccacgt cacaaactcc accccctcat tatcatattg gcttcaatcc
3901 aaaataaggt ata
//
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pDC316质粒载体应用举例
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