| 质粒名称: | pET30a-His6-LOXCAT |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 140084 |
| 存储实验室: | Vamsi Mootha |
|
载体骨架基本信息 |
|
| 载体名称: | pET30a |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 8838 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | A fusion of lactate oxidase from A. viridans and catalase from E. coli,His6-LOXC |
| 物种来源: | Lactate oxidase from A. viridans and catalase from E. coli |
| 插入大小: | 3600 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | T7 |
| 标签/融合蛋白: | His6（主干上的N终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NdeI（销毁后未知） |
| 3' 克隆位点: | XhoI（销毁后未知） |
| 5' 测序引物 | TTGTGAGCGGATAACAATTCCCC |
| 3' 测序引物 | AAGGGGTTATGCTAGTTATTGCTCAG |
|
文献引用方法 |
|
| 材料和方法部分: | pET30a-His6-LOXCAT was a gift from Vamsi Mootha (Addgene plasmid # 140084 ; http://n2t.net/addgene:140084 ; RRID:Addgene_140084) |
| 参考文献部分: | An engineered enzyme that targets circulating lactate to alleviate intracellular NADH:NAD(+) imbalance. Patgiri A, Skinner OS, Miyazaki Y, Schleifer G, Marutani E, Shah H, Sharma R, Goodman RP, To TL, Robert Bao X, Ichinose F, Zapol WM, Mootha VK. Nat Biotechnol. 2020 Jan 13. pii: 10.1038/s41587-019-0377-7. doi: 10.1038/s41587-019-0377-7. 10.1038/s41587-019-0377-7 PubMed 31932725 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pET30a-His6-LOXCAT 8834 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase
(CAT) that converts lactate into pyruvate without producing H2O2..
ACCESSION .
VERSION .
KEYWORDS pET30a-His6-LOXCAT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8834)
AUTHORS Patgiri A, Skinner OS, Miyazaki Y, Schleifer G, Marutani E, Shah H,
Sharma R, Goodman RP, To TL, Robert Bao X, Ichinose F, Zapol WM,
Mootha VK
TITLE An engineered enzyme that targets circulating lactate to alleviate
intracellular NADH:NAD(+) imbalance.
JOURNAL Nat Biotechnol. 2020 Jan 13. pii: 10.1038/s41587-019-0377-7. doi:
10.1038/s41587-019-0377-7.
PUBMED 31932725
REFERENCE 2 (bases 1 to 8834)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8834
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 12..467
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(99..118)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 309..330
/label=F1ori-F
/note="F1 origin, forward primer"
CDS complement(559..1374)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 1282..1301
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 1366..1385
/label=pENTR-R
/note="pENTR vectors, reverse primer"
rep_origin 1496..2084
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1985..2004
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2238..2255
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 2270..2409
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(2335..2354)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2495..2517
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(2511..2702)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(3511..4593)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 4555..4574
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(4594..4671)
/gene="lacI"
/label=lacI promoter
primer_bind 4877..4896
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind 4984..5003
/label=T7
/note="T7 promoter, forward primer"
promoter 4984..5002
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 5003..5027
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 5042..5064
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 5075..5092
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 5102..5119
/codon_start=1
/product="thrombin recognition and cleavage site"
/label=thrombin site
/translation="LVPRGS"
CDS 5126..5170
/codon_start=1
/product="affinity and epitope tag derived from pancreatic
ribonuclease A"
/label=S-Tag
/translation="KETAAAKFERQHMDS"
CDS 5186..5200
/codon_start=1
/product="enterokinase recognition and cleavage site"
/label=enterokinase site
/translation="DDDDK"
CDS 8678..8695
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(8748..8766)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 8762..8809
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
ORIGIN
1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
541 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
601 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
661 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
721 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
781 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
841 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
901 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
961 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
1021 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
1081 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
1141 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
1201 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
1261 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
1321 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc
1381 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac
1441 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
1501 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
1561 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
1621 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
1681 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
1741 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
1801 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
1861 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
1921 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
1981 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
2041 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
2101 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
2161 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
2221 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
2281 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat
2341 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
2401 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
2461 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
2521 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
2581 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
2641 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
2701 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
2761 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
2821 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
2881 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
2941 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
3001 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
3061 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
3121 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg
3181 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct
3241 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc
3301 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt
3361 tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
3421 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
3481 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
3541 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
3601 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
3661 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
3721 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
3781 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
3841 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
3901 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
3961 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
4021 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
4081 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
4141 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
4201 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
4261 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
4321 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
4381 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
4441 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
4501 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
4561 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
4621 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
4681 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
4741 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
4801 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
4861 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
4921 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga
4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
5041 ttttgtttaa ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg
5101 tctggtgcca cgcggttctg gtatgaaaga aaccgctgct gctaaattcg aacgccagca
5161 catggacagc ccagatctgg gtaccgacga cgacgacaag gccatggctg atatcggatc
5221 cgaattcgag ctcagccaac acaatgagaa gaatccgcat caacatcaaa gcccgctgca
5281 cgatagcagc gaggcgaagc cgggtatgga cagcctggcg ccggaggacg gtagccaccg
5341 tccggcggcg gaaccgaccc cgccgggcgc gcagccgacc gcgccgggta gcctgaaggc
5401 gccggatacc cgtaacgaga aactgaacag cctggaagac gtgcgtaagg gtagcgagaa
5461 ctacgcgctg accaccaacc agggcgttcg tatcgcggac gatcaaaaca gcctgcgtgc
5521 gggcagccgt ggtccgaccc tgctggagga ttttattctg cgtgaaaaaa tcacccactt
5581 cgaccacgag cgtattccgg aacgtattgt gcatgcgcgt ggcagcgcgg cgcatggtta
5641 cttccagccg tataagagcc tgagcgacat taccaaagcg gattttctga gcgacccgaa
5701 caagatcacc ccggtgtttg ttcgtttcag caccgtgcaa ggtggcgcgg gtagcgcgga
5761 taccgttcgt gacattcgtg gctttgcgac caaattctac accgaggaag gcatctttga
5821 tctggttggt aacaacaccc cgatcttctt tattcaggat gcgcacaagt ttccggactt
5881 cgtgcacgcg gttaaaccgg agccgcactg ggcgattccg cagggtcaaa gcgcgcacga
5941 taccttctgg gactacgtga gcctgcaacc ggaaaccctg cacaacgtta tgtgggcgat
6001 gagcgaccgt ggtatcccgc gtagctatcg taccatggag ggctttggta ttcacacctt
6061 ccgtctgatc aacgcggaag gcaaggcgac ctttgtgcgt ttccactgga aaccgctggc
6121 gggtaaagcg agcctggttt gggacgaggc gcagaaactg accggtcgtg acccggattt
6181 ccaccgtcgt gaactgtggg aggcgattga agcgggcgat tttccggaat acgagctggg
6241 tttccaactg atcccggagg aagatgaatt caagtttgac ttcgatctgc tggacccgac
6301 caaactgatc ccggaggaac tggtgccggt tcagcgtgtg ggcaagatgg ttctgaaccg
6361 taacccggac aacttctttg cggaaaacga gcaagcggcg tttcacccgg gccacattgt
6421 gccgggtctg gatttcacca acgacccgct gctgcagggt cgtctgttta gctataccga
6481 tacccaaatc agccgtctgg gtggcccgaa ctttcacgag atcccgatta accgtccgac
6541 ctgcccgtac cacaacttcc agcgtgatgg catgcaccgt atgggtattg acaccaaccc
6601 ggcgaactat gagccgaaca gcatcaacga caactggccg cgtgaaaccc cgccgggtcc
6661 gaagcgtggt ggctttgaga gctaccaaga gcgtgtggag ggtaacaaag ttcgtgagcg
6721 tagcccgagc ttcggcgaat actatagcca cccgcgtctg ttttggctga gccagacccc
6781 gttcgaacaa cgtcacatcg ttgatggttt tagcttcgag ctgagcaagg tggttcgtcc
6841 gtatattcgt gaacgtgtgg ttgatcagct ggcgcacatc gacctgaccc tggcgcaagc
6901 ggtggcgaaa aacctgggca ttgagctgac cgacgatcag ctgaacatca ccccgccgcc
6961 ggatgttaac ggtctgaaga aagacccgag cctgagcctg tacgcgattc cggacggcga
7021 tgtgaagggt cgtgtggttg cgatcctgct gaacgatgag gttcgtagcg cggacctgct
7081 ggcgatcctg aaggcgctga aggcgaaagg cgtgcacgcg aaactgctgt atagccgtat
7141 gggcgaagtg accgcggacg atggtaccgt tctgccgatt gcggcgacct ttgcgggtgc
7201 gccgagcctg accgttgacg cggtgatcgt tccgtgcggc aacatcgcgg acattgcgga
7261 taacggtgac gcgaactact atctgatgga agcgtacaag cacctgaaac cgattgcgct
7321 ggcgggcgat gcgcgtaagt ttaaagcgac catcaaaatt gcggaccagg gcgaggaagg
7381 tatcgtggag gcggatagcg cggacggtag cttcatggac gaactgctga ccctgatggc
7441 ggcgcaccgt gtttggagcc gtatcccgaa gattgataaa attccggcgg cgagcggtgc
7501 gggtggcagc gagggtggcg gtagcgaagg cggtaccagc ggtgcgacca acaacaacga
7561 cattgagtac aacgcgccga gcgaaattaa gtacatcgat gtggttaaca cctatgacct
7621 ggaggaagag gcgagcaaag tggttccgca cggcggtttt aactacatcg cgggcgcgag
7681 cggtgatgag tggaccaagc gtgcgaacga ccgtgcgtgg aagcacaaac tgctgtatcc
7741 gcgtctggcg caggatgtgg aggcgccgga caccagcacc gaaattctgg gtcacaagat
7801 caaagcgccg ttcattatgg cgccgattgc ggcgcatggt ctggcgcaca ccaccaaaga
7861 ggcgggtacc gcgcgtgcgg ttagcgaatt cggcaccatt atgagcatca gcgcgtacag
7921 cggtgcgacc tttgaagaaa ttagcgaggg tctgaacggc ggtccgcgtt ggttccaaat
7981 ttatatggcg aaggacgatc agcaaaaccg tgatatcctg gacgaagcga aaagcgatgg
8041 cgcgaccgcg atcattctga ccgcggacag caccgtgagc ggtaaccgtg accgtgatgt
8101 gaagaacaaa tttgtttacc cgttcggcat gccgattgtt cagcgttatc tgcgtggcac
8161 cgcggagggt atgagcctga acaacattta cggtgcgagc aagcaaaaaa tcagcccgcg
8221 tgacattgaa gaaattgcgg gtcacagcgg tctgccggtg ttcgttaagg gtatccagca
8281 cccggaagac gcggatatgg cgattaaacg tggcgcgagc ggtatctggg ttagcaacca
8341 cggtgcgcgt caactgtatg aagcgccggg tagctttgat accctgccgg cgattgcgga
8401 acgtgttaac aagcgtgtgc cgatcgtttt cgacagcggc gtgcgtcgtg gtgaacacgt
8461 tgcgaaagcg ctggcgagcg gtgcggatgt ggttgcgctg ggtcgtccgg tgctgtttgg
8521 tctggcgctg ggcggttggc agggtgcgta cagcgtgctg gattatttcc aaaaagacct
8581 gacccgtgtt atgcagctga ccggtagcca aaatgttgag gacctgaagg gcctggacct
8641 gtttgataac ccgtatggct atgagtatta actcgagcac caccaccacc accactgaga
8701 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
8761 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
8821 aactatatcc ggat
//
