质粒名称: | GFP-TAF1 |
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出品公司: | 爱迪基因 |
目录编号: | 65395 |
存储实验室: | Kyle Miller |
载体骨架基本信息 |
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载体名称: | pcDNA6.2/N-EmGFP-DEST |
载体出品公司: | invitrogen |
载体抗性: | 氨苄青霉素 |
空载体大小: | 5847 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | 杀稻瘟菌素 |
高拷贝/低拷贝: | 低拷贝 |
生长条件: | 30 ℃ |
插入基因信息 |
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插入基因名称: | TAF1 |
物种来源: | H. sapiens (human) |
插入大小: | 5616 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子 | CMV |
标签/融合蛋白: |
EmGFP(主干上的N终端)
V5(主干上的C终端)
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克隆方法: | Gateway克隆 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | EGFP_C_primer |
3' 测序引物 | TK polyA reverse primer |
文献引用方法 |
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材料和方法部分: | GFP-TAF1 was a gift from Kyle Miller (Addgene plasmid # 65395 ; http://n2t.net/addgene:65395 ; RRID:Addgene_65395) |
参考文献部分: | Screen identifies bromodomain protein ZMYND8 in chromatin recognition of transcription-associated DNA damage that promotes homologous recombination. Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M, Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM. Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114. 10.1101/gad.252189.114 PubMed 25593309 |
买家导航
GFPTAF1载体基本信息
GFPTAF1载体图谱质粒图谱和多克隆位点信息
GFPTAF1载体简介
GFPTAF1载体序列
LOCUS GFP-TAF1 11569 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION GFP-tagged BRD protein, which can be used to be expressed in mammaiian cells.. ACCESSION . VERSION . KEYWORDS GFP-TAF1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11569) AUTHORS Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M, Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM TITLE Screen identifies bromodomain protein ZMYND8 in chromatin recognition of transcription-associated DNA damage that promotes homologous recombination. JOURNAL Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114. PUBMED 25593309 REFERENCE 2 (bases 1 to 11569) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11569 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(11..28) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(182..770) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(262..281) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(941..1801) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1564..1583 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1802..1906) /gene="bla" /label=AmpR promoter primer_bind complement(1981..2000) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 2172..2551 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2552..2755 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2706..2726 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2752..2776 /label=LNCX /note="Human CMV promoter, forward primer" CDS 2880..3596 /codon_start=1 /product="Emerald GFP" /label=EmGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(2925..2946) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3186..3205) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3533..3554 /label=EGFP-C /note="EGFP, forward primer" protein_bind 3612..3636 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(9331..9355) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 9363..9404 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" primer_bind complement(9444..9463) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 9488..9536 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 9738..10166 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9825..9844) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 10035..10056 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(10175..10195) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 10180..10509 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 10360..10495 /label=SV40 ori /note="SV40 origin of replication" primer_bind 10422..10441 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 10557..10604 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 10623..11021 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" polyA_signal 11179..11300 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(11216..11235) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 11270..11289 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(11349..11365) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(11349..11365) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(11362..11384) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 11373..11389 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(11397..11427) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11442..11463 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." ORIGIN 1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 1921 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag 1981 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga 2041 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa 2101 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag 2161 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt 2221 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg 2281 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac 2341 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt 2401 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa 2461 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta 2521 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg 2581 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg 2641 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc 2701 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt 2761 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca 2821 ccgggaccga tccagcctcc ggactctaga ggatccctac cggtgatatc ctcgagacca 2881 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 2941 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 3001 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc 3061 tcgtgaccac cttcacctac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc 3121 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 3181 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 3241 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 3301 agctggagta caactacaac agccacaagg tctatatcac cgccgacaag cagaagaacg 3361 gcatcaaggt gaacttcaag acccgccaca acatcgagga cggcagcgtg cagctcgccg 3421 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 3481 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 3541 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggct 3601 cgagcccatc aacaagtttg tacaaaaaag caggcttcga aggagataga accaattctc 3661 taaggaaata cttaaccatg gtcgacatgg gacccggctg cgatttgctg ctgcggacag 3721 cagctaccat cactgctgcc gccatcatgt cagacacgga cagcgacgaa gattccgctg 3781 gaggcggccc attttcttta gcgggtttcc ttttcggcaa catcaatgga gccgggcagc 3841 tggaggggga aagcgtcttg gatgatgaat gtaagaagca cttggcaggc ttgggggctt 3901 tggggctggg cagcctgatc actgaactca cggcaaatga agaattgacc gggactgacg 3961 gtgccttggt aaatgatgaa gggtgggtta ggagtacaga agatgctgtg gactattcag 4021 acatcaatga ggtggcagaa gatgaaagcc gaagatacca gcagacgatg gggagcttgc 4081 agcccctttg ccactcagat tatgatgaag atgactatga tgctgattgt gaagacattg 4141 attgcaagtt gatgcctcct ccacctccac ccccgggacc aatgaagaag gataaggacc 4201 aggattctat tactggtgag aaagtggact tcagtagttc ctctgactca gaatctgaga 4261 tgggacctca ggaagcaaca caggcagaat ctgaagatgg aaagctgacc cttccattgg 4321 ctgggattat gcagcatgat gccaccaagc tgttgccaag tgtcacagaa ctttttccag 4381 aatttcgacc tggaaaggtg ttacgttttc tacgtctttt tggaccaggg aagaatgtcc 4441 catctgtttg gcggagtgct cggagaaaga ggaagaagaa gcaccgtgag ctgatacagg 4501 aagagcagat ccaggaggtg gagtgctcag tagaatcaga agtcagccag aagtctttgt 4561 ggaactacga ctacgctcca ccaccacctc cagagcagtg tctctctgat gatgaaatca 4621 cgatgatggc tcctgtggag tccaaatttt cccaatcaac tggagatata gataaagtga 4681 cagataccaa accaagagtg gctgagtggc gttatgggcc tgcccgactg tggtatgata 4741 tgctgggtgt ccctgaagat ggcagtgggt ttgactatgg cttcaaactg agaaagacag 4801 aacatgaacc tgtgataaaa tctagaatga tagaggaatt taggaaactt gaggaaaaca 4861 atggcactga tcttctggct gatgaaaact tcctgatggt gacacagctg cattgggagg 4921 atgatatcat ctgggatggg gaggatgtca aacacaaagg gacaaaacct cagcgtgcaa 4981 gcctggcagg ctggcttcct tctagcatga ctaggaatgc gatggcttac aatgttcagc 5041 aaggttttgc agccactctt gatgatgaca aaccttggta ctccattttt cccattgaca 5101 atgaggatct ggtatatgga cgctgggagg acaatatcat ttgggatgct caggccatgc 5161 cccggctgtt ggaacctcct gttttgacac ttgatcccaa tgatgagaac ctcattttgg 5221 aaattcctga tgagaaggaa gaggccacct ctaactcccc ctccaaggag agtaagaagg 5281 aatcatctct gaagaagagt cgaattctct tagggaaaac aggagtcatc aaggaggaac 5341 cacagcagaa catgtctcag ccagaagtga aagatccatg gaatctctcc aatgatgagt 5401 attattatcc caagcaacag ggtcttcgag gcacctttgg agggaatatt atccagcatt 5461 caattcctgc tgtggaatta cggcagccct tctttcccac ccacatgggg cccatcaaac 5521 tccggcagtt ccatcgccca cctctgaaaa agtactcatt tggtgcactt tctcagccag 5581 gtccccactc agtccaacct ttgctaaagc acatcaaaaa aaaggccaag atgagagaac 5641 aagagaggca agcttcaggt ggtggagaga tgttttttat gcgcacacct caggacctca 5701 caggcaaaga tggtgatctt attcttgcag aatatagtga ggaaaatgga cccttaatga 5761 tgcaggttgg catggcaacc aagataaaga actattataa acggaaacct ggaaaagatc 5821 ctggagcacc agattgtaaa tatggggaaa ctgtttactg ccatacatct cctttcctgg 5881 gttctctcca tcctggccaa ttgctgcaag catttgagaa caaccttttt cgtgctccaa 5941 tttatcttca taagatgcca gaaactgatt tcttgatcat tcggacaaga cagggttact 6001 atattcggga attagtggat atttttgtgg ttggccagca gtgtcccttg tttgaagttc 6061 ctgggcctaa ctccaaaagg gccaatacgc atattcgaga ctttctacag gtttttattt 6121 accgcctttt ctggaaaagt aaagatcggc cacggaggat acgaatggaa gatataaaaa 6181 aagcctttcc ttcccattca gaaagcagca tccggaagag gctaaagctc tgcgctgact 6241 tcaaacgcac agggatggac tcaaactggt gggtgcttaa gtctgatttt cgtttaccaa 6301 cggaagaaga gatcagagct atggtgtcac cagagcagtg ctgtgcttat tatagcatga 6361 tagctgcaga gcaacgactg aaggatgctg gctatggtga gaaatccttt tttgctccag 6421 aagaagaaaa tgaggaagat ttccagatga agattgatga tgaagttcgc actgcccctt 6481 ggaacaccac aagggccttc attgctgcca tgaagggcaa gtgtctgcta gaggtgactg 6541 gggtggcaga tcccacgggg tgtggtgaag gattctccta tgtgaagatt ccaaacaaac 6601 caacacagca gaaggatgat aaagaaccgc agccagtgaa gaagacagtg acaggaacag 6661 atgcagacct tcgtcgcctt tccctgaaaa atgccaagca acttctacgt aaatttggtg 6721 tgcctgagga agagattaaa aagttgtccc gctgggaagt gattgatgtg gtgcgcacaa 6781 tgtcaacaga acaggctcgt tctggagagg ggcccatgag taaatttgcc cgtggatcaa 6841 ggttttctgt ggctgagcat caagagcgtt acaaagagga atgtcagcgc atctttgacc 6901 tacagaacaa ggttctgtca tcaactgaag tcttatcaac tgacacagac agcagctcag 6961 ctgaagatag tgactttgaa gaaatgggaa agaacattga gaacatgttg cagaacaaga 7021 aaaccagctc tcagctttca cgtgaacggg aggaacagga gcggaaggaa ctacagcgaa 7081 tgctactggc agcaggctca gcagcatccg gaaacaatca cagagatgat gacacagctt 7141 ccgtgactag ccttaactct tctgccactg gacgctgtct caagatttat cgcacgtttc 7201 gagatgaaga ggggaaagag tatgttcgct gtgagacagt ccgaaaacca gctgtcattg 7261 atgcctatgt gcgcatacgg actacaaaag atgaggaatt cattcgaaaa tttgcccttt 7321 ttgatgaaca acatcgggaa gagatgcgaa aagaacggcg gaggattcaa gagcaactga 7381 ggcggcttaa gaggaaccag gaaaaggaga agcttaaggg tcctcctgag aagaagccca 7441 agaaaatgaa ggagcgtcct gacctaaaac tgaaatgtgg ggcatgtggt gccattggac 7501 acatgaggac taacaaattc tgccccctct attatcaaac aaatgcgcca ccttccaacc 7561 ctgttgccat gacagaagaa caggaggagg agttggaaaa gacagtcatt cataatgata 7621 atgaagaact tatcaaggtt gaagggacca aaattgtctt ggggaaacag ctaattgaga 7681 gtgcggatga ggttcgcaga aaatctctgg ttctcaagtt tcctaaacag cagcttcctc 7741 caaagaagaa acggcgagtt ggaaccactg ttcactgtga ctatttgaat agacctcata 7801 agtccatcca ccggcgccgc acagacccta tggtgacgct gtcgtccatc ttggagtcta 7861 tcatcaatga catgagagat cttccaaata catacccttt ccacactcca gtcaatgcaa 7921 aggttgtaaa ggactactac aaaatcatca ctcggccaat ggacctacaa acactccgcg 7981 aaaacgtgcg taaacgcctc tacccatctc gggaagagtt cagagagcat ctggagctaa 8041 ttgtgaaaaa tagtgcaacc tacaatgggc caaaacactc attgactcag atctctcaat 8101 ccatgctgga tctctgtgat gaaaaactca aagagaaaga agacaaatta gctcgcttag 8161 agaaagctat caaccccttg ctggatgatg atgaccaagt ggcgttttct ttcattctgg 8221 acaacattgt cacccagaaa atgatggcag ttccagattc ttggccattt catcacccag 8281 ttaataagaa atttgttcca gattattaca aagtgattgt caatccaatg gatttagaga 8341 ccatacgtaa gaacatctcc aagcacaagt atcagagtcg ggagagcttt ctggatgatg 8401 taaaccttat tctggccaac agtgttaagt ataatggacc tgagagtcag tatactaaga 8461 ctgcccagga gattgtgaac gtctgttacc agacattgac tgagtatgat gaacatttga 8521 ctcaacttga gaaggatatt tgtactgcta aagaagcagc tttggaggaa gcagaattag 8581 aaagcctgga cccaatgacc ccagggccct acacgcctca gcctcctgat ttgtatgata 8641 ccaacacatc cctcagtatg tctcgagatg cctctgtatt tcaagatgag agcaatatgt 8701 ctgtcttgga tattcccagt gccactccag aaaagcaggt aacacaggaa ggtgaagatg 8761 gagatggtga tcttgcagat gaagaggaag gaactgtaca acagcctcaa gccagtgtcc 8821 tgtatgagga tttgcttatg tctgaaggag aagatgatga ggaagatgct gggagtgatg 8881 aagaaggaga caatcctttc tctgctatcc agctgagtga aagtggaagt gactctgatg 8941 tgggatctgg tggaataaga cccaaacaac cccgcatgct tcaggagaac acaaggatgg 9001 acatggaaaa tgaagaaagc atgatgtcct atgagggaga cggtggggag gcttcccatg 9061 gtttggagga tagcaacatc agttatggga gctatgagga gcctgatccc aagtcgaaca 9121 cccaagacac aagcttcagc agcatcggtg ggtatgaggt atcagaggag gaagaagatg 9181 aggaggagga agagcagcgc tctgggccga gcgtactaag ccaggtccac ctgtcagagg 9241 acgaggagga cagtgaggat ttccactcca ttgctgggga cagtgacttg gactctgatg 9301 aattatcgcg gccgcactcg agatatctag acccagcttt cttgtacaaa gtggttgata 9361 acgggaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat 9421 gagtttaaac gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc 9481 gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa 9541 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg 9601 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc 9661 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcagatctgc gcagctgggg 9721 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 9781 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 9841 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 9901 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 9961 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 10021 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 10081 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 10141 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga 10201 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 10261 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 10321 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 10381 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 10441 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 10501 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg 10561 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa 10621 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa 10681 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg 10741 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg 10801 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg 10861 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc 10921 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga 10981 ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agcacttcgt ggccgaggag 11041 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc 11101 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 11161 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat 11221 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 11281 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg 11341 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 11401 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 11461 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 11521 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgct //
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GFPTAF1载体质粒应用举例
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