pET-28a-EGFP

价格:800元

联系方式:I47-825O-882O

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pET28a-EGFP载体基本信息

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载体名称: pET-28a-EGFP,pET28a-EGFP
质粒类型: 大肠表达质粒;大肠荧光质粒;大肠绿色质粒
启动子: T7/LAC
载体大小: 6050bp
5' 测序引物及序列: T7 (TAATACGACTCACTATAGGG)
3' 测序引物及序列: T7-ter (TGCTAGTTATTGCTCAGCGG)
载体标签:
N-6×His, N-Thrombin, N-EGFP, C-6×His
载体抗性: 卡那霉素(kanamycin)
克隆菌株: DH5α 等
培养条件: 37℃,有氧,LB
表达宿主:
BL21(DE3)
诱导方式: IPTG or lactose and its analogues

pET28a-EGFP载体质粒图谱和多克隆位点信息

pET28a- EGFP质粒图谱
pET28a- EGFP质粒图谱1

pET28a-EGFP载体简介

pET28a-EGFP载体序列

LOCUS       pET-28a-EGFP                6050 bp ds-DNA     circular SYN 12-JUL-2017
DEFINITION  synthetic circular DNA
KEYWORDS    pET28a-EGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6050)
  TITLE     Direct Submission
  JOURNAL   Exported 2015-4-17 
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, July 12, 2017
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      12..467
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 ori
                     f1 bacteriophage origin of replication; arrow indicates
                     direction of (+) strand synthesis"
     CDS             complement(560..1375)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="aph(3')-Ia"
                     /note="KanR
                     confers resistance to kanamycin in bacteria or G418
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1497..2085
                     /direction=RIGHT
                     /note="ori"
                     /note="ori
                     high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    2271..2413
                     /note="bom"
                     /note="bom
                     basis of mobility region from pBR322"
     CDS             complement(2515..2706)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3515..4597)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /note="lacI"
                     /note="lacI
                     The lac repressor binds to the lac operator to inhibit
                     transcription in E. coli. This inhibition can be relieved
                     by adding lactose or isopropyl-beta-D-thiogalactopyranoside
                     (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(4598..4675)
                     /gene="lacI"
                     /note="lacI lacI promoter"
                     /note="lacI promoter"
     promoter        4984..5002
                     /note="T7 promoter"
                     /note="T7 promoter
                     promoter for bacteriophage T7 RNA polymerase"
     CDS             5083..5100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     CDS             5131..5847
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="enhanced GFP"
                     /note="EGFP
                     mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    5848..5893
                     /note="MCS"
     CDS             5894..5911
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis affinity tag"
                     /note="6xHis"

      

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pET28a-EGFP载体应用举例

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