| 质粒名称: | p415-PtrpC-Cas9-TtrpC-CYC1t |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 68059 |
| 存储实验室: | Christian Hong |
|
载体骨架基本信息 |
|
| 载体名称: | pRS415 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 11431 bp |
| 载体修饰: | -- |
| 载体类型: | 酵母表达，CRISPR；N.crassa，真菌 |
| 筛选标记: | LEU2 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | humanized Cas9 |
| 物种来源: | H、 智者（人类）；克拉萨酵母菌，化脓酵母菌，其他真菌 |
| 插入大小: | 4140 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | p415-PtrpC-Cas9-TtrpC-CYC1t was a gift from Christian Hong (Addgene plasmid # 68059 ; http://n2t.net/addgene:68059 ; RRID:Addgene_68059) |
| 参考文献部分: | Efficient gene editing in Neurospora crassa with CRISPR technology. Matsu-ura Toru, Baek Mokryun, Kwon Jungin and Hong Christian. Fungal Biology and Biotechnology 2015, 2:4 10.1186/s40694-015-0015-1 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS p415-PtrpC-Cas9-TtrpC-CYC1t 11249 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Express humanized Cas9 under the control of trpC promoter and
terminator from A. nidulans..
ACCESSION .
VERSION .
KEYWORDS p415-PtrpC-Cas9-TtrpC-CYC1t
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11249)
AUTHORS Matsu-ura Toru, Baek Mokryun, Kwon Jungin, Hong Christian
TITLE Efficient gene editing in Neurospora crassa with CRISPR technology
JOURNAL Fungal Biology and Biotechnology 2015, 2:4
REFERENCE 2 (bases 1 to 11249)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11249
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(10..28)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
misc_feature 70..573
/label=CEN/ARS
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"
promoter 610..714
/gene="bla"
/label=AmpR promoter
CDS 715..1575
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(933..952)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1746..2334
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2235..2254
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2488..2505
/label=L4440
/note="L4440 vector, forward primer"
promoter 2658..2688
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2696..2712
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2701..2723
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2720..2736
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2720..2736
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 2755..2775
/label=T3
/note="T3 promoter, forward primer"
promoter 2757..2775
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
CDS 3170..7273
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 7286..7306
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(8138..8157)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(8139..8157)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(8167..8184)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(8167..8183)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8176..8198)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 8328..8783
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8415..8434)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8625..8646
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 9084..9491
/gene="S. cerevisiae LEU2"
/label=LEU2 promoter
CDS 9492..10586
/codon_start=1
/gene="S. cerevisiae LEU2"
/product="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
/label=LEU2
/note="yeast auxotrophic marker"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
DLGGSNSTTEVGDAVAEEVKKILA"
primer_bind complement(11080..11099)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 11199..11221
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
ORIGIN
1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
2761 aaccctcact aaagggaaca aaagctggag aagatgatat tgaaggagca tttttgggct
2821 tggctggagc tagtggaggt caacaatgaa tgcctatttt ggtttagtcg tccaggcggt
2881 gagcacaaaa tttgtgtcgt ttgacaagat ggttcattta ggcaactggt cagatcatcc
2941 ccacttgtag cagtagcggc ggcgctcgaa gtgtgactct tattagcaga caggaacgag
3001 gacattatta tcatctgctg cttggtgcac gataacttgg tgcgtttgtc aagcaaggta
3061 agtgaacgac ccggtcatac cttcttaagt tcgcccttcc tccctttatt tcagattcaa
3121 tctgacttac ctattctacc gaagcactag tggatccccc gggaaaaaaa tggacaagaa
3181 gtactccatt gggctcgata tcggcacaaa cagcgtcggt tgggccgtca ttacggacga
3241 gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa
3301 gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag ccacgcggct
3361 caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga
3421 gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc tggaggagtc
3481 ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga
3541 cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga agcttgtaga
3601 cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt
3661 tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact
3721 ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga tcaacgcatc
3781 cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa
3841 cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc ttatcgccct
3901 gtcactcggg ctgaccccca actttaaatc taacttcgac ctggccgaag atgccaagct
3961 tcaactgagc aaagacacct acgatgatga tctcgacaat ctgctggccc agatcggcga
4021 ccagtacgca gacctttttt tggcggcaaa gaacctgtca gacgccattc tgctgagtga
4081 tattctgcga gtgaacacgg agatcaccaa agctccgctg agcgctagta tgatcaagcg
4141 ctatgatgag caccaccaag acttgacttt gctgaaggcc cttgtcagac agcaactgcc
4201 tgagaagtac aaggaaattt tcttcgatca gtctaaaaat ggctacgccg gatacattga
4261 cggcggagca agccaggagg aattttacaa atttattaag cccatcttgg aaaaaatgga
4321 cggcaccgag gagctgctgg taaagcttaa cagagaagat ctgttgcgca aacagcgcac
4381 tttcgacaat ggaagcatcc cccaccagat tcacctgggc gaactgcacg ctatcctcag
4441 gcggcaagag gatttctacc cctttttgaa agataacagg gaaaagattg agaaaatcct
4501 cacatttcgg ataccctact atgtaggccc cctcgcccgg ggaaattcca gattcgcgtg
4561 gatgactcgc aaatcagaag agaccatcac tccctggaac ttcgaggaag tcgtggataa
4621 gggggcctct gcccagtcct tcatcgaaag gatgactaac tttgataaaa atctgcctaa
4681 cgaaaaggtg cttcctaaac actctctgct gtacgagtac ttcacagttt ataacgagct
4741 caccaaggtc aaatacgtca cagaagggat gagaaagcca gcattcctgt ctggagagca
4801 gaagaaagct atcgtggacc tcctcttcaa gacgaaccgg aaagttaccg tgaaacagct
4861 caaagaagac tatttcaaaa agattgaatg tttcgactct gttgaaatca gcggagtgga
4921 ggatcgcttc aacgcatccc tgggaacgta tcacgatctc ctgaaaatca ttaaagacaa
4981 ggacttcctg gacaatgagg agaacgagga cattcttgag gacattgtcc tcacccttac
5041 gttgtttgaa gatagggaga tgattgaaga acgcttgaaa acttacgctc atctcttcga
5101 cgacaaagtc atgaaacagc tcaagaggcg ccgatataca ggatgggggc ggctgtcaag
5161 aaaactgatc aatgggatcc gagacaagca gagtggaaag acaatcctgg attttcttaa
5221 gtccgatgga tttgccaacc ggaacttcat gcagttgatc catgatgact ctctcacctt
5281 taaggaggac atccagaaag cacaagtttc tggccagggg gacagtcttc acgagcacat
5341 cgctaatctt gcaggtagcc cagctatcaa aaagggaata ctgcagaccg ttaaggtcgt
5401 ggatgaactc gtcaaagtaa tgggaaggca taagcccgag aatatcgtta tcgagatggc
5461 ccgagagaac caaactaccc agaagggaca gaagaacagt agggaaagga tgaagaggat
5521 tgaagagggt ataaaagaac tggggtccca aatccttaag gaacacccag ttgaaaacac
5581 ccagcttcag aatgagaagc tctacctgta ctacctgcag aacggcaggg acatgtacgt
5641 ggatcaggaa ctggacatca atcggctctc cgactacgac gtggatcata tcgtgcccca
5701 gtcttttctc aaagatgatt ctattgataa taaagtgttg acaagatccg ataaaaatag
5761 agggaagagt gataacgtcc cctcagaaga agttgtcaag aaaatgaaaa attattggcg
5821 gcagctgctg aacgccaaac tgatcacaca acggaagttc gataatctga ctaaggctga
5881 acgaggtggc ctgtctgagt tggataaagc cggcttcatc aaaaggcagc ttgttgagac
5941 acgccagatc accaagcacg tggcccaaat tctcgattca cgcatgaaca ccaagtacga
6001 tgaaaatgac aaactgattc gagaggtgaa agttattact ctgaagtcta agctggtctc
6061 agatttcaga aaggactttc agttttataa ggtgagagag atcaacaatt accaccatgc
6121 gcatgatgcc tacctgaatg cagtggtagg cactgcactt atcaaaaaat atcccaagct
6181 tgaatctgaa tttgtttacg gagactataa agtgtacgat gttaggaaaa tgatcgcaaa
6241 gtctgagcag gaaataggca aggccaccgc taagtacttc ttttacagca atattatgaa
6301 ttttttcaag accgagatta cactggccaa tggagagatt cggaagcgac cacttatcga
6361 aacaaacgga gaaacaggag aaatcgtgtg ggacaagggt agggatttcg cgacagtccg
6421 gaaggtcctg tccatgccgc aggtgaacat cgttaaaaag accgaagtac agaccggagg
6481 cttctccaag gaaagtatcc tcccgaaaag gaacagcgac aagctgatcg cacgcaaaaa
6541 agattgggac cccaagaaat acggcggatt cgattctcct acagtcgctt acagtgtact
6601 ggttgtggcc aaagtggaga aagggaagtc taaaaaactc aaaagcgtca aggaactgct
6661 gggcatcaca atcatggagc gatcaagctt cgaaaaaaac cccatcgact ttctcgaggc
6721 gaaaggatat aaagaggtca aaaaagacct catcattaag cttcccaagt actctctctt
6781 tgagcttgaa aacggccgga aacgaatgct cgctagtgcg ggcgagctgc agaaaggtaa
6841 cgagctggca ctgccctcta aatacgttaa tttcttgtat ctggccagcc actatgaaaa
6901 gctcaaaggg tctcccgaag ataatgagca gaagcagctg ttcgtggaac aacacaaaca
6961 ctaccttgat gagatcatcg agcaaataag cgaattctcc aaaagagtga tcctcgccga
7021 cgctaacctc gataaggtgc tttctgctta caataagcac agggataagc ccatcaggga
7081 gcaggcagaa aacattatcc acttgtttac tctgaccaac ttgggcgcgc ctgcagcctt
7141 caagtacttc gacaccacca tagacagaaa gcggtacacc tctacaaagg aggtcctgga
7201 cgccacactg attcatcagt caattacggg gctctatgaa acaagaatcg acctctctca
7261 gctcggtgga gacagcaggg ctgaccccaa gaagaagagg aaggtgtgat tttggacctc
7321 gagacgcgta cttaacgtta ctgaaatcat caaacagctt gacgaatctg gatataagat
7381 cgttggtgtc gatgtcagct ccggagttga gacaaatggt gttcaggatc tcgataagat
7441 acgttcattt gtccaagcag caaagagtgc cttctagtga tttaatagct ccatgtcaac
7501 aagaataaaa cgcgtttcgg gtttacctct tccagataca gctcatctgc aatgcattaa
7561 tgcattggac ctcgcaaccc tagtacgccc ttcaggctcc ggcgaagcag aagaatagct
7621 tagcagagtc tattttcatt ttcgggagac gagatcaagc agatcaacgg tcgtcaagag
7681 acctacgaga ctgaggaatc cgctcttggc tccacgcgac tatatatttg tctctaattg
7741 tactttgaca tgctcctctt ctttactctg atagcttgac tatgaaaatt ccgtcaccag
7801 cccctgggtt cgcaaagata attgcactgt ttcttccttg aactctcaag cctacaggac
7861 acacattcat cgtaggtata aacctcgaaa atcattccta ctaagatggg tatacaatag
7921 taaccatggt tgcctagtga atgctccgta acacccaata cgccggccga aactttttta
7981 caactctcct atgagtcgtt tacccagaat gcacaggtac acttgtttag aggtaatcct
8041 tctttctaga gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac
8101 gctcgaaggc tttaatttgc ggccggtacc caattcgccc tatagtgagt cgtattacgc
8161 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
8221 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
8281 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag
8341 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
8401 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
8461 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
8521 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
8581 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
8641 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
8701 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
8761 caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
8821 atttcacacc gcatatcgac cggtcgagga gaacttctag tatatctaca tacctaatat
8881 tattgcctta ttaaaaatgg aatcccaaca attacatcaa aatccacatt ctcttcaaaa
8941 tcaattgtcc tgtacttcct tgttcatgtg tgttcaaaaa cgttatattt ataggataat
9001 tatactctat ttctcaacaa gtaattggtt gtttggccga gcggtctaag gcgcctgatt
9061 caagaaatat cttgaccgca gttaactgtg ggaatactca ggtatcgtaa gatgcaagag
9121 ttcgaatctc ttagcaacca ttattttttt cctcaacata acgagaacac acaggggcgc
9181 tatcgcacag aatcaaattc gatgactgga aattttttgt taatttcaga ggtcgcctga
9241 cgcatatacc tttttcaact gaaaaattgg gagaaaaagg aaaggtgaga gcgccggaac
9301 cggcttttca tatagaatag agaagcgttc atgactaaat gcttgcatca caatacttga
9361 agttgacaat attatttaag gacctattgt tttttccaat aggtggttag caatcgtctt
9421 actttctaac ttttcttacc ttttacattt cagcaatata tatatatata tttcaaggat
9481 ataccattct aatgtctgcc cctaagaaga tcgtcgtttt gccaggtgac cacgttggtc
9541 aagaaatcac agccgaagcc attaaggttc ttaaagctat ttctgatgtt cgttccaatg
9601 tcaagttcga tttcgaaaat catttaattg gtggtgctgc tatcgatgct acaggtgttc
9661 cacttccaga tgaggcgctg gaagcctcca agaaggctga tgccgttttg ttaggtgctg
9721 tgggtggtcc taaatggggt accggtagtg ttagacctga acaaggttta ctaaaaatcc
9781 gtaaagaact tcaattgtac gccaacttaa gaccatgtaa ctttgcatcc gactctcttt
9841 tagacttatc tccaatcaag ccacaatttg ctaaaggtac tgacttcgtt gttgtcagag
9901 aattagtggg aggtatttac tttggtaaga gaaaggaaga cgatggtgat ggtgtcgctt
9961 gggatagtga acaatacacc gttccagaag tgcaaagaat cacaagaatg gccgctttca
10021 tggccctaca acatgagcca ccattgccta tttggtcctt ggataaagct aatgttttgg
10081 cctcttcaag attatggaga aaaactgtgg aggaaaccat caagaacgaa ttccctacat
10141 tgaaggttca acatcaattg attgattctg ccgccatgat cctagttaag aacccaaccc
10201 acctaaatgg tattataatc accagcaaca tgtttggtga tatcatctcc gatgaagcct
10261 ccgttatccc aggttccttg ggtttgttgc catctgcgtc cttggcctct ttgccagaca
10321 agaacaccgc atttggtttg tacgaaccat gccacggttc tgctccagat ttgccaaaga
10381 ataaggtcaa ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa ttgtcattga
10441 acttgcctga agaaggtaag gccattgaag atgcagttaa aaaggttttg gatgcaggta
10501 tcagaactgg tgatttaggt ggttccaaca gtaccaccga agtcggtgat gctgtcgccg
10561 aagaagttaa gaaaatcctt gcttaaaaag attctctttt tttatgatat ttgtacataa
10621 actttataaa tgaaattcat aatagaaacg acacgaaatt acaaaatgga atatgttcat
10681 agggtagacg aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg
10741 atgtaaagga atacaggtaa gcaaattgat actaatggct caacgtgata aggaaaaaga
10801 attgcacttt aacattaata ttgacaagga ggagggcacc acacaaaaag ttaggtgtaa
10861 cagaaaatca tgaaactatg attcctaatt tatatattgg aggattttct ctaaaaaaaa
10921 aaaaatacaa caaataaaaa acactcaatg acctgaccat ttgatggagt ttaagtcaat
10981 accttcttga accatttccc ataatggtga aagttccctc aagaatttta ctctgtcaga
11041 aacggcctta acgacgtagt cgatatggtg cactctcagt acaatctgct ctgatgccgc
11101 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
11161 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
11221 gttttcaccg tcatcaccga aacgcgcga
//
