| 质粒名称: | EC.SurA.pTYB1.a21-281&390-428.wt |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 12607 |
| 存储实验室: | David McKay |
|
载体骨架基本信息 |
|
| 载体名称: | pTYB1 |
| 载体出品公司: | New England Biolabs |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7280 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Escherichia coli surA delta PPIase domain 2,SurA(deltaP2) |
| 物种来源: | E. coli |
| 插入大小: | 900 bp |
| 基因突变: | residues 282-389 deleted |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | intein（插入件上的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NdeI(未破坏) |
| 3' 克隆位点: | KpnI (未破坏) |
| 5' 测序引物 | T7 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | EC.SurA.pTYB1.a21-281&390-428.wt was a gift from David McKay (Addgene plasmid # 12607 ; http://n2t.net/addgene:12607 ; RRID:Addgene_12607) |
| 参考文献部分: | The periplasmic molecular chaperone protein SurA binds a peptide motif that is characteristic of integral outer membrane proteins. Bitto E, McKay DB. J Biol Chem. 2003 Dec 5. 278(49):49316-22. 10.1074/jbc.M308853200 PubMed 14506253 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS EC.SurA.pTYB1.a21-281&390-428.wt 8332 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS EC.SurA.pTYB1.a21-281&390-428.wt
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8332)
AUTHORS Bitto E, McKay DB
TITLE The periplasmic molecular chaperone protein SurA binds a peptide
motif that is characteristic of integral outer membrane proteins.
JOURNAL J Biol Chem. 2003 Dec 5. 278(49):49316-22.
PUBMED 14506253
REFERENCE 2 (bases 1 to 8332)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8332
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 35..139
/gene="bla"
/label=AmpR promoter
CDS 140..1000
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(358..377)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin complement(1042..1555)
/direction=LEFT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1237..1258)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 1449..1468
/label=F1ori-R
/note="F1 origin, reverse primer"
rep_origin 1666..2254
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2155..2174
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2408..2425
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(2447..2466)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2607..2629
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(2623..2814)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(3371..4453)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 4415..4434
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(4454..4531)
/gene="lacI"
/label=lacI promoter
terminator 4684..4770
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 4867..4953
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 5050..5136
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 5233..5319
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 5416..5502
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 5637..5656
/label=T7
/note="T7 promoter, forward primer"
promoter 5637..5655
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 5656..5680
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 5695..5717
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 6631..7449
/codon_start=1
/gene="Saccharomyces cerevisiae VMA1"
/product="5' region of the intein from the yeast Vma1
subunit of the vacuolar ATPase (Chong et al., 1998)"
/label=Sce VMA intein 5' region
/translation="CFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRE
TMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEV
ITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEARD
LSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSD
RATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRG"
CDS 7905..7916
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
CDS 8026..8181
/codon_start=1
/gene="Bacillus circulans chiA"
/product="chitin binding domain from chitinase A1 (Watanabe
et al., 1994)"
/label=CBD
/translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
PALWQLQ"
primer_bind complement(8246..8264)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 8260..8307
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
ORIGIN
1 aactacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
61 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
121 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
181 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
241 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
301 ccttgagagt tttcgccccg aagaacgttc tccaatgatg agcactttta aagttctgct
361 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
421 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
481 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
541 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
601 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
661 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
721 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
781 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
841 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
901 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
961 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
1021 atatatactt tagattgatt taccccggtt gataatcaga aaagccccaa aaacaggaag
1081 attgtataag caaatattta aattgtaaac gttaatattt tgttaaaatt cgcgttaaat
1141 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
1201 tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
1261 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
1321 ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
1381 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
1441 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
1501 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaaag
1561 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
1621 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
1681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
1741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
1801 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
1861 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
1921 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
1981 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
2041 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
2101 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
2161 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
2221 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
2281 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
2341 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
2401 cgagcgcagc gagtcagtga gcgaggaagc tatggtgcac tctcagtaca atctgctctg
2461 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
2521 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
2581 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
2641 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gcagcgattc
2701 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt
2761 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc
2821 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat
2881 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa
2941 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcc
3001 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt
3061 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat
3121 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc
3181 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt
3241 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
3301 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat
3361 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
3421 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
3481 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
3541 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg
3601 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac
3661 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
3721 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
3781 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
3841 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
3901 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
3961 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
4021 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
4081 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
4141 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
4201 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
4261 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
4321 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
4381 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
4441 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac
4501 cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac
4561 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg
4621 aatggtgcat gccggcatgc cgccctttcg tcttcaagaa ttaattccca attccccagg
4681 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
4741 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
4801 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa ttaattcccc
4861 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
4921 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg
4981 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gaattaattc
5041 cccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
5101 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt
5161 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggaattaa
5221 ttccccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
5281 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
5341 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggaat
5401 taattcccca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
5461 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
5521 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
5581 aattggggat cggaattaat tcccggttta aaccggggat ctcgatcccg cgaaattaat
5641 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
5701 taactttaag aaggagatat acatatggcc ccccaggtag tcgataaagt cgcagccgtc
5761 gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc ggtaaaactg
5821 aacgctgctc aggcaaggca gcaacttcct gatgacgcga cgctgcgcca ccaaatcatg
5881 gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg agtgaaaatc
5941 tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa catgacgctg
6001 gatcagatgc gcagccgtct ggcttacgat ggactgaact acaacaccta tcgtaaccag
6061 atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg tcgcatcacc
6121 atcctgccgc aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa cgacgccagc
6181 actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac ctctgatcag
6241 gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa cggcgctgat
6301 ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg cggccagatg
6361 ggctggggcc gtattcagga gttgcccggg atcttcgccc aggcattaag caccgcgaag
6421 aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct gaaagttaac
6481 gacctgcgcg gcgaaagcaa aaatatctcg gacgctgcgc agaaagatcg tgcataccgc
6541 atgctgatga accgtaagtt ctcggaagaa gcagcaagct ggatgcagga acaacgtgcc
6601 agcgcctacg ttaaaatcct gagcaacggg tgctttgcca agggtaccaa tgttttaatg
6661 gcggatgggt ctattgaatg tattgaaaac attgaggttg gtaataaggt catgggtaaa
6721 gatggcagac ctcgtgaggt aattaaattg cccagaggaa gagaaactat gtacagcgtc
6781 gtgcagaaaa gtcagcacag agcccacaaa agtgactcaa gtcgtgaagt gccagaatta
6841 ctcaagttta cgtgtaatgc gacccatgag ttggttgtta gaacacctcg tagtgtccgc
6901 cgtttgtctc gtaccattaa gggtgtcgaa tattttgaag ttattacttt tgagatgggc
6961 caaaagaaag cccccgacgg tagaattgtt gagcttgtca aggaagtttc aaagagctac
7021 ccaatatctg aggggcctga gagagccaac gaattagtag aatcctatag aaaggcttca
7081 aataaagctt attttgagtg gactattgag gccagagatc tttctctgtt gggttcccat
7141 gttcgtaaag ctacctacca gacttacgct ccaattcttt atgagaatga ccactttttc
7201 gactacatgc aaaaaagtaa gtttcatctc accattgaag gtccaaaagt acttgcttat
7261 ttacttggtt tatggattgg tgatggattg tctgacaggg caactttttc ggttgattcc
7321 agagatactt ctttgatgga acgtgttact gaatatgctg aaaagttgaa tttgtgcgcc
7381 gagtataagg acagaaaaga accacaagtt gccaaaactg ttaatttgta ctctaaagtt
7441 gtcagaggta atggtattcg caataatctt aatactgaga atccattatg ggacgctatt
7501 gttggcttag gattcttgaa ggacggtgtc aaaaatattc cttctttctt gtctacggac
7561 aatatcggta ctcgtgaaac atttcttgct ggtctaattg attctgatgg ctatgttact
7621 gatgagcatg gtattaaagc aacaataaag acaattcata cttctgtcag agatggtttg
7681 gtttcccttg ctcgttcttt aggcttagta gtctcggtta acgcagaacc tgctaaggtt
7741 gacatgaatg tcaccaaaca taaaattagt tatgctattt atatgtctgg tggagatgtt
7801 ttgcttaacg ttctttcgaa gtgtgccggc tctaaaaaat tcaggcctgc tcccgccgct
7861 gcttttgcac gtgagtgccg cggattttat ttcgagttac aagaattgaa ggaagacgat
7921 tattatggga ttactttatc tgatgattct gatcatcagt ttttgcttgg atcccaggtt
7981 gtcgtccatg catgcggtgg cctgaccggt ctgaactcag gcctcacgac aaatcctggt
8041 gtatccgctt ggcaggtcaa cacagcttat actgcgggac aattggtcac atataacggc
8101 aagacgtata aatgtttgca gccccacacc tccttggcag gatgggaacc atccaacgtt
8161 cctgccttgt ggcagcttca atgactgcag gaaggggatc cggctgctaa caaagcccga
8221 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
8281 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg at
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：5aa SunTag VP64 EVD
- 下一篇：pBAtC
