| 质粒名称: | pSC300 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31820 |
| 存储实验室: | Yossef Av-Gay |
|
载体骨架基本信息 |
|
| 载体名称: | pSODIT |
| 载体出品公司: | Koen De Smet Imperial College Medical School |
| 载体抗性: | 潮霉素 |
| 空载体大小: | 5497 bp |
| 完整质粒大小: | 6220 bp |
| 载体类型: | 细菌表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GFP (lacking ATG start codon) |
| 插入大小: | 723 bp |
| 启动子 | SOD |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | HindIII(未破坏) |
| 3' 克隆位点: | HindIII (未破坏) |
| 5' 测序引物 | NA |
| 3' 测序引物 | GFP-R |
|
文献引用方法 |
|
| 材料和方法部分: | pSC300 was a gift from Yossef Av-Gay (爱迪基因 plasmid # 31820) |
| 参考文献部分: | Monitoring promoter activity and protein localization in Mycobacterium spp. using green fluorescent protein. Cowley SC, Av-Gay Y. Gene. 2001 Feb 21;264(2):225-31. 10.1016/S0378-1119(01)00336-5 PubMed 11250077 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSC300 6220 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pSC300
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6220)
AUTHORS Cowley SC, Av-Gay Y
TITLE Monitoring promoter activity and protein localization in
Mycobacterium spp. using green fluorescent protein.
JOURNAL Gene. 2001 Feb 21;264(2):225-31.
PUBMED 11250077
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pSC300|
FEATURES Location/Qualifiers
source 1..6220
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 432..443
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
CDS 503..1219
/codon_start=1
/product="Aequoria victoria green fluorescent protein"
/label=GFP
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
primer_bind complement(533..561)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 1156..1176
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
primer_bind complement(1235..1251)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1236..1252)
/label=pBluescriptKS
/note="For pBluescript vector"
rep_origin 5451..6039
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5940..5959
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6193..6210
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacc agatctggct
241 cgcaccgcgg tggcggccgc tctagacagc ctgggggcgt cctggtagcc tatgccgtcc
301 gccctggggc atcgacccca aggtcgttgt tgcgacgcga gcggtcatgg agcagggttg
361 acttgtcaag ctagagccag cccatcgcgt gggaggcacc cgcgcgaaaa gaaacatcgg
421 acgatcattt catcgaagga aggaatgccg tgggatcccc cgggctgcag gaattcgata
481 tcaagctgta ccggtagaaa aaatgagtaa aggagaagaa cttttcactg gagttgtccc
541 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
601 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
661 acctgttcca tggccaacac ttgtcactac tttgacttat ggtgttcaat gcttttcaag
721 atacccagat catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt
781 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
841 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
901 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
961 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
1021 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
1081 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
1141 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
1201 ggatgaacta tacaaatagc attcgtaagc ttatcgatac cgtcgacctc gagggggggc
1261 ccccggggat catcgagccg agaacgttat cgaagttggt catgtgtaat cccctcgttt
1321 gaactttgga ttaagcgtag atacaccctt ggacaagcca gttggattcg gagacaagca
1381 aattcagcct taaaaagggc gaggccctgc ggtggtggaa caccgcaggg cctctaaccg
1441 ctcgacgcgc tgcaccaacc agcccgcgaa cggctggcag ccagcgtaag gcgcggctca
1501 tcgggcggcg ttcgccacga tgtcctgcac ttcgagccaa gcctcgaaca cctgctggtg
1561 tgcacgactc acccggttgt tgacaccgcg cgcggccgtg cgggctcggt ggggcggctc
1621 tgtcgccctt gccagcgtga gtagcgcgta cctcacctcg cccaacaggt cgcacacagc
1681 cgattcgtac gccataaagc caggtgagcc caccagctcc gtaagttcgg gcgctgtgtg
1741 gctcgtaccc gcgcattcag gcggcagggg gtctaacggg tctaaggcgg cgtgtacggc
1801 cgccacagcg gctctcagcg gcccggaaac gtcctcgaaa cgacgcatgt gttcctcctg
1861 gttggtacag gtggttgggg gtgctcggct gtcgctggtg ttccaccacc agggctcgac
1921 gggagagcgg gggagtgtgc agttgtgggg tggcccctca gcgaaatatc tgacttggag
1981 ctcgtgtcgg accatacacc ggtgattaat cgtggtctac taccaagcgt gagccacgtc
2041 gccgacgaat ttgagcagct ctggctgccg tactggccgc tggcaagcga cgatctgctc
2101 gaggggatct accgccaaag ccgcgcgtcg gccctaggcc gccggtacat cgaggcgaac
2161 ccaacagcgc tggcaaacct gctggtcgtg gacgtagacc atccagacgc agcgctccga
2221 gcgctcagcg cccgggggtc ccatccgctg cccaacgcga tcgtgggcaa tcgcgccaac
2281 ggccacgcac acgcagtgtg ggcactcaac gcccctgttc cacgcaccga atacgcgcgg
2341 cgtaagccgc tcgcatacat ggcggcgtgc gccgaaggcc ttcggcgcgc cgtcgacggc
2401 gaccgcagtt actcaggcct catgaccaaa aaccccggcc acatcgcctg ggaaacggaa
2461 tggctccact cagatctcta cacactcagc cacatcgagg ccgagctcgg cgcgaacatg
2521 ccaccgccgc gctggcgtca gcagaccacg tacaaagcgg ctccgacgcc gctagggcgg
2581 aattgcgcac tgttcgattc cgtcaggttg tgggcctatc gtcccgccct catgcggatc
2641 tacctgccga cccggaacgt ggacggactc ggccgcgcga tctatgccga gtgccacgcg
2701 cgaaacgccg aattcccgtg caacgacgtg tgtcccggac cgctaccgga cagcgaggtc
2761 cgcgccatcg ccaacagcat ttggcgttgg atcacaacca agtcgcgcat ttgggcggac
2821 gggatcgtgg tctacgaggc cacactcagt gcgcgccagt cggccatctc gcggaagggc
2881 gcagcagcgc gcacggcggc gagcacagtt gcgcggcgcg caaagtccgc gtcagccatg
2941 gaggcattgc tatgagcgac ggctacagcg acggctacaa ccggcagccg actgtccgca
3001 aaaagcggcg cgtgaccgcc gccgaaggcg ctcgaatcac cggactatcc gaacgccacg
3061 tcgtccggct cgtggcgcag gaacgcagcg agtggctcgc cgagcaggct gcacgccgcg
3121 aacgcatccg cgcctatcac gacgacgagg gccactcttg gccgcaaacg gccaaacatt
3181 tcgggctgca tctggacacc gttaagcgac tcggctatcg ggcgaggaaa gagcgtgcgg
3241 cagaacagga agcggctcaa aaggcccaca acgaagccga caatccaccg ctgttctaac
3301 gcaattgggg agcgggtgtc gcgggggttc cgtggggggt tccgttgcaa cgggtcggac
3361 aggtaaaagt cctggtagac gctagttttc tggtttgggc catgcctgtc tcgttgcgtg
3421 tttcgttgcg cccgttttga ataccagcca gacgagacgg ggttctacga atcttggtcg
3481 ataccaagcc atttccgctg aatatcgggg agctcaccgc cagaatcggt ggttgtggtg
3541 atgtacgtgg cgaactccgt tgtagtgcct gtggtggcat ccgtggccac tctcgttgca
3601 cggttcgttg tgccgttaca ggccccgttg acagctcacc gaacgtagtt aaaacatgct
3661 ggtcaaacta ggtttaccaa cgatacgagt cagctcatct agggccagtt ctaggcgttg
3721 ttcgttgcgc ggttcgttgc gcatgtttcg tgtggttgct agatggctcc gcaaccacac
3781 gcttcgaggt tgagtgcttc cagcacgggc gcgatccaga agaacttcgt cgtgcgactg
3841 tcctcgttga tccttgccga gctgggatgg aagcccggcc gacccaccct ggaggagatg
3901 atcgaggatg ccagggcctt tcacgcccgc cgctgctgag cgtccgccgc cgggcccgca
3961 ccgccgtcgg ccggcccgct ccgggctcgc agcagcgggc ttcggcgcgg gcccggggct
4021 cccgggccgc cgggcggggc tccgcccggc ggccgccggg ggccgggggc ggcgccgggc
4081 ggcccggggc gtcaggcgcc gggggcggtg tccggcggcc cccagaggaa ctgcgccagt
4141 tcctccggat cggtgaagcc ggagagatcc agcggggtct cctcgaacac ctcgaagtcg
4201 tgcaggaagg tgaaggcgag cagttcgcgg gcgaagtcct cggtccgctt ccactgcgcc
4261 ccgtcgagca gcgcggccag gatctcgcgg tcgccccgga aggcgttgag atgcagttgc
4321 accaggctgt agcgggagtc tcccgcatag acgtcggtga agtcgacgat cccggtgacc
4381 tcggtcgcgg ccaggtccac gaagatgttg gtcccgtgca ggtcgccgtg gacgaaccgg
4441 ggttcgcggc cggccagcag cgtgtccacg tccggcagcc agtcctccag gcggtccagc
4501 agccggggcg agaggtagcc ccacccgcgg tggtcctcga cggtcgccgc gcggcgttcc
4561 cgcagcagtt ccgggaagac ctcggaatgg ggggtgagca cggtgttccc ggtcagcggc
4621 accctgtgca gccggccgag cacccggccg agttcgcggg ccagggcgag cagcgcgttc
4681 cggtcggtcg tgccgtccat cgcggaccgc caggtggtgc cggtcatccg gctcatcacc
4741 aggtagggcc acggccaggc tccggtgccg ggccgcagct cgccgcggcc gaggaggcgg
4801 ggcaccggca ccggggcgtc cgccaggacc gcgtacgcct ccgactccga cgcgaggctc
4861 tccggaccgc accagtgctc gccgaacagc ttgatcaccg ggccgggctc gccgaccagt
4921 acggggttgg tgctctcgcc gggcacccgc agcaccggcg gcaccggcag cccgagctcc
4981 tccagggctc ggcgggccag cggctcccag aattcctggt cgttccgcag gctcgcgtag
5041 gaatcatccg aatcaatacg gtcgagaagt aacagggatt cttgtgtcac agcggacctc
5101 tattcacagg gtacgggccg gcttaattcc gcacggccgg tcgcgacacg gcctgtccgc
5161 accgcggatc aggcgttgac gatgacgggc tggtcggcca cgtcggggag tcaggcaact
5221 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
5281 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
5341 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
5401 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
5461 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
5521 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
5581 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
5641 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
5701 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
5761 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
5821 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
5881 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
5941 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
6001 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
6061 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
6121 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
6181 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
//
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