| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.70 ; pGL4.70[hRluc] |
| 质粒类型: | 信号通路报告载体；哺乳动物载体；海肾荧光素酶报告载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 3522 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | hRluc |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 潮霉素(Hygromycin) |
| 克隆菌株: | TOP10等常规菌株 |
| 宿主细胞（系）: | HEK293等 |
| 备注: | pGL4.70[hRluc]载体是信号通路报告载体；无启动子；含海肾 荧光素酶报告基因hRluc。 |
| 产品目录号: | E6881 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pGL4.70[hRluc]载体pGL4.70质粒基本信息
pGL4.70[hRluc]质粒图谱pGL4.70载体图谱和pGL4.70[hRluc]载体序列pGL4.70质粒序列多克隆位点信息
pGL4.70[hRluc]质粒pGL4.70载体简介
The pGL4.70[hRluc] Vector encodes the hRluc gene (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. The pGL4.70[hRluc] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows for cloning of a promoter of choice.
pGL4.70[hRluc]质粒序列pGL4.70载体序列
LOCUS AY738226 3522 bp DNA circular SYN 08-NOV-2004
DEFINITION Renilla luciferase reporter vector pGL4.70[hRluc], complete
sequence.
ACCESSION AY738226
VERSION AY738226.1 GI:55535630
KEYWORDS .
SOURCE Renilla luciferase reporter vector pGL4.70[hRluc]
ORGANISM Renilla luciferase reporter vector pGL4.70[hRluc]
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3522)
AUTHORS Almond,B., Chauvin,F. and Browning,J.
TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
Corporation
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3522)
AUTHORS Almond,B., Chauvin,F. and Browning,J.
TITLE Direct Submission
JOURNAL Submitted (31-AUG-2004) Scientific Communications, Promega
Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES Location/Qualifiers
source 1..3522
/organism="Renilla luciferase reporter vector
pGL4.70[hRluc]"
/mol_type="other DNA"
/db_xref="taxon:296663"
misc_feature 1..70
/note="multiple cloning site region"
CDS 100..1035
/codon_start=1
/transl_table=11
/product="luciferase hRluc2"
/protein_id="AAV52877.1"
/db_xref="GI:55535631"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
misc_feature 1067..1288
/note="SV40 late poly(A) region"
primer_bind 1356..1375
/note="reporter vector primer 4 (RVPrimer4)"
rep_origin 1613
/note="ColE1-derived"
gene complement(2404..3264)
/gene="ampR"
CDS complement(2404..3264)
/gene="ampR"
/note="AmpR"
/codon_start=1
/transl_table=11
/product="synthetic beta-lactamase"
/protein_id="AAV52878.1"
/db_xref="GI:55535632"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3369..3522
/regulatory_class="polyA_signal_sequence"
/note="synthetic; transcriptional pause site"
primer_bind complement(3471..3490)
/note="reporter vector primer 3 (RVPrimer3)"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
1021 aagaacgagc agtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
1081 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
1141 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
1201 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
1261 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt
1321 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
1381 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg
1441 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
1501 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
1561 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
1621 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
1681 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
1741 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
1801 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
1861 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
1921 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
1981 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
2041 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
2101 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
2161 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
2221 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
2281 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
2341 tctaaagtat atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag
2401 tggttaccag tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc
2461 catagtggcc tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg
2521 ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat
2581 gaaccagcca gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat
2641 ccagtctatg agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg
2701 aagagttgtg gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc
2761 gttcaactct ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa
2821 tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc
2881 gctcatggta atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt
2941 ttccgtgacc ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag
3001 ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt
3061 gctcatcatc gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag
3121 atccagttcg atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac
3181 cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc
3241 gacacgaaaa tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca
3301 gggttactag tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga
3361 caggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
3421 cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat
3481 aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ct
//
