质粒名称: | pMXs-Oct3/4 |
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出品公司: | Addgene |
目录编号: | 13366 |
存储实验室: | Shinya Yamanaka |
载体骨架基本信息 |
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载体名称: | pMXs |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | 4600 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳动物表达,逆转录病毒 |
筛选标记: | -- |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | POU domain, class 5, transcription factor 1,Oct3/4 |
物种来源: | M. musculus (mouse) |
插入大小: | 1134 bp |
基因突变: | None |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII(N端) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | pMX-S1811 |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pMXs-Oct3/4 was a gift from Shinya Yamanaka (Addgene plasmid # 13366 ; http://n2t.net/addgene:13366 ; RRID:Addgene_13366) |
参考文献部分: | Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi K, Yamanaka S. Cell. 2006 Aug 25. 126(4):663-76. 10.1016/j.cell.2006.07.024 PubMed 16904174 |
买家导航
pMXsOct3/4载体基本信息
pMXsOct3/4载体图谱质粒图谱和多克隆位点信息
pMXsOct3/4载体简介
pMXsOct3/4载体序列
LOCUS pMXs-Oct3/4 5959 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION Retroviral expression of mouse Oct3/4. ACCESSION . VERSION . KEYWORDS pMXs-Oct3/4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5959) AUTHORS Takahashi K, Yamanaka S TITLE Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. JOURNAL Cell. 2006 Aug 25. 126(4):663-76. PUBMED 16904174 REFERENCE 2 (bases 1 to 5959) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5959 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 120..589 /note="long terminal repeat from Moloney murine leukemia virus" protein_bind 906..930 /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(2159..2183) /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 2234..2608 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" primer_bind complement(2280..2296) /label=pBMN 5' /note="MMLV sequence, for inserts in pBMN retroviral vector" CDS complement(2618..3034) /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind complement(2634..2650) /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" primer_bind complement(2666..2688) /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" misc_feature 3093..3450 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" promoter 4061..4165 /gene="bla" /label=AmpR promoter CDS 4166..5026 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4384..4403) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5190..5778 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5679..5698 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5932..5949 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg 61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt 121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga 181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact 241 cagtcaatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg 301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca 361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc 421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg 481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag 541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct 601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa 661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt 721 ttctggagac taaataaaat cttttatttt atcgtcgacc actgtgctgg cggccgctcg 781 agtttaaata gtacataatg gatttcctta cgcgaaatac gggcagacat ggcctgcccg 841 gttattatta tttttgacac cagaccaact ggtaatggta gcgaccggcg ctcagctgga 901 atatcaccac tttgtacaag aaagctgggt tgatcaacag catcactgag cttctttccc 961 catcccaccc cccacccctg ttgtgctttt aatccctcct cagtaaaaga atttaacccc 1021 aaagctccag gttctcttgt ctacctccct tgccttggct cacagcatcc ccagggaggg 1081 ctggtgcctc agtttgaatg catgggagag cccagagcag tgacgggaac agagggaaag 1141 gcctcgccct caggaaaagg gactgagtag agtgtggtga agtgggggct tccatagcct 1201 ggggtgccaa agtggggacc tgggggcaga ggaaaggata cagccccccc tgggaaaggt 1261 gtccctgtag cctcatactc ttctcgttgg gaatactcaa tacttgatct tttgcccttc 1321 tggcgccggt tacagaacca tactcgaacc acatccttct ctagcccaag ctgattggcg 1381 atgtgagtga tctgctgtag ggagggcttc gggcacttca gaaacatggt ctccagactc 1441 cacctcacac ggttctcaat gctagttcgc tttctcttcc gggcctgcac cagggtctcc 1501 gatttgcata tctcctgaag gttctcattg ttgtcggctt cctccaccca cttctccagc 1561 aggggccgca gcttacacat gttcttaagg ctgagctgca aggcctcgaa gcgacagatg 1621 gtggtctggc tgaacacctt tccaaagaga acgcccaggg tgagccccac gtcggcctgg 1681 gtgtacccca aggtgatcct cttctgcttc agcagcttgg caaactgttc tagctccttc 1741 tgcagggctt tcatgtcctg ggactcctcg ggagttggtt ccaccttctc caacttcacg 1801 gcattggggc ggtcggcaca gggctcagag gaggttccct ctgagttgct ttccactcgt 1861 gctcctgcct ggccctcagg ctgcaaagtc tccacgccaa cttgggggac taggcccagt 1921 ccaacctgag gtccacagta tgccatccct ccgcagaact cgtatgcggg cggacatggg 1981 gagatcccca atacctctga gcctggtccg attccaggcc cacctggagg cccttggaag 2041 cttagccagg ttcgagaatc cacccagccc ggctccagcc ctgctgaccc atcaccccca 2101 cctggtgggg gtgagaaggc gaagtctgaa gccaggtgtc cagccatggg gaaggtggag 2161 cctgcttttt tgtacaaact tgtgatttcc cgtaccacca cactgggatc cttaattaac 2221 tagctagatc cggcagtcta gaggatggtc cacccccggg gtcggcagcc ttcacgtggg 2281 cggcgtgtat ccaagctgcg atgccgtcta ctttgagggc ggtgggggtg gtcagcagga 2341 ctgtgtaagg tcctttccag cgaggttcta ggttcttagt ctggtgtcgg cggacccaca 2401 ctgtgtcgcc gactcggtaa gggtgaggta ccaccggtcg gtccagttgt tcttggtagg 2461 ctgccgccag aggtctccag acttcgtgct ggactaagta gagagcctgt aagtgagctt 2521 ggagagaggg gctgttagta actcttgtca tgtcagggtc agggaagttt acaaggggcg 2581 ggggtgcccc atataagatc tcatatggcc atatgggggc gcctagagaa ggagtgaggg 2641 ctggataaag ggaggatcga ggcggggtcg aacgaggagg ttcaaggggg agagacgggg 2701 cggatggagg aagaggaggc ggaggcttag ggtgtacaaa gggcttgacc cagggagggg 2761 ggtcaaaagc caaggcttcc caggtcacga tgtaggggac ctggtctggg tgtccatgcg 2821 ggccaggtga aaagaccttg atcttaacct gggtgatgag gtctcggtta aaggtgccgt 2881 ctcgcggcca tccgacgtta aaggttggcc attctgcaga gcagaaggta acccaacgtc 2941 tcttcttgac atctaccgac tggttgtgag cgatccgctc gacatctttc cagtgaccta 3001 aggtcaaact taagggagtg gtaacagtct ggccctaatt ttcagacaaa tacagaaaca 3061 cagtcagaca gagacaacac agaacgatgc tgcagcagac aagacgcgcg gcgcggcttc 3121 ggtcccaaac cgaaagcaaa aattcagacg gaggcgggaa ctgttttagg ttctcgtctc 3181 ctaccagaac cacatatccc tcctattagg ggggtgcacc aaagagtcca aaacgatcgg 3241 gatttttgga ctcaggtcgg gccacaaaaa cggcccccga agtccctggg acgtctccca 3301 gggttgcggc cgggtgttcc gaactcgtca gttccaccac gggtccgcca gatacagagc 3361 tagttagcta actagtaccg acgcaggcgc ataaaatcag tcatagacac tagacaatcg 3421 gacagacaca gataagttgc tggccagctt acctcccggt ggtgggtcgg tggtccctgg 3481 gcaggggtct cccgatcccg gacgagcccc caaatgaaag acccccgctg acgggtagtc 3541 aatcactcag aggagaccct cccaaggaac agcgagacca caagtcggat gcaactgcaa 3601 gagggtttat tggatacacg ggtacccggg cgactcagtc aatcggagga ctggcgcccc 3661 gagtgagggg ttgtgggctc ttttattgag ctcggggagc agaagcgcgc gaacagaagc 3721 gagaagcgaa ctgattggtt agttcaaata aggcacaggg tcatttcagg tccttggggc 3781 accctggaaa catctgatgg ttctctagaa actgctgagg gctggaccgc atctggggac 3841 catctgttct tggccctgag ccggggcagg aactgcttac cacagatatc ctgtttggcc 3901 catattcagc tgttccatct gttcctgacc ttgatctgaa cttttctatt ctcagttatg 3961 tatttttcca tgccttgcaa aatggcgtta cttaagctag cttgccaaac ctacaggtgg 4021 ggtctttcat tatgcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 4081 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 4141 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 4201 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 4261 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 4321 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 4381 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 4441 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 4501 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 4561 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 4621 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 4681 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 4741 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 4801 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 4861 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 4921 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 4981 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 5041 ttactcatat atactttaga ttgatttgcg gccggccgca aaaggatcta ggtgaagatc 5101 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 5161 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 5221 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 5281 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 5341 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 5401 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 5461 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 5521 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 5581 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 5641 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 5701 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 5761 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 5821 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 5881 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 5941 gtgagcgagg aagcggaag //
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