| 质粒名称: | pEGFP-TYR |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 32781 |
| 存储实验室: | Ruth Halaban |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-N |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4700 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Tyrosinase |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | EGFP（主干上的C端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV forward |
| 3' 测序引物 | EGFP_N |
|
文献引用方法 |
|
| 材料和方法部分: | pEGFP-TYR was a gift from Ruth Halaban (Addgene plasmid # 32781 ; http://n2t.net/addgene:32781 ; RRID:Addgene_32781) |
| 参考文献部分: | Endoplasmic reticulum retention is a common defect associated with tyrosinase-negative albinism. Halaban R, Svedine S, Cheng E, Smicun Y, Aron R, Hebert DN. Proc Natl Acad Sci U S A. 2000 May 23;97(11):5889-94. 10.1073/pnas.97.11.5889 PubMed 10823941 |
买家导航
pEGFPTYR载体基本信息
pEGFPTYR载体图谱质粒图谱和多克隆位点信息
pEGFPTYR载体简介
pEGFPTYR载体序列
LOCUS pEGFP-TYR 6424 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pEGFP-TYR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6424)
AUTHORS Halaban R, Svedine S, Cheng E, Smicun Y, Aron R, Hebert DN
TITLE Endoplasmic reticulum retention is a common defect associated with
tyrosinase-negative albinism.
JOURNAL Proc Natl Acad Sci U S A. 2000 May 23;97(11):5889-94.
PUBMED 10823941
REFERENCE 2 (bases 1 to 6424)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6424
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 2364..2373
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2370..3089
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2415..2436)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2676..2695)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3023..3044
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 3212..3333
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3249..3268)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3303..3322
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(3340..3795)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3477..3498)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3689..3708
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3822..3926
/gene="bla"
/label=AmpR promoter
promoter 3928..4285
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3951..3971)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 4136..4271
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4198..4217
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4320..5114
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4374..4393)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4984..5003
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(5302..5321)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 5346..5393
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5722..6310
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6211..6230
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attccgggaa gaatgctcct ggctgttttg
661 tactgcctgc tgtggagttt ccagacctcc gctggccatt tccctagagc ctgtgtctcc
721 tctaagaacc tgatggagaa ggaatgctgt ccaccgtgga gcggggacag gagtccctgt
781 ggccagcttt caggcagagg ttcctgtcag aatatccttc tgtccaatgc accacttggg
841 cctcaatttc ccttcacagg ggtggatgac cgggagtcgt ggccttccgt cttttataat
901 aggacctgcc agtgctctgg caacttcatg ggattcaact gtggaaactg caagtttggc
961 ttttggggac caaactgcac agagagacga ctcttggtga gaagaaacat cttcgatttg
1021 agtgccccag agaaggacaa attttttgcc tacctcactt tagcaaagca taccatcagc
1081 tcagactatg tcatccccat agggacctat ggccaaatga aaaatggatc aacacccatg
1141 tttaacgaca tcaatattta tgacctcttt gtctggatcc attattatgt gtcaatggat
1201 gcactgcttg ggggatatga aatctggaga gacattgatt ttgcccatga agcaccagct
1261 tttctgcctt ggcatagact cttcttgttg cggtgggaac aagaaatcca gaagctgaca
1321 ggagatgaaa acttcactat tccatattgg gactggcggg atgcagaaaa gtgtgacatt
1381 tgcacagatg agtacatggg aggtcagcac cccacaaatc ctaacttact cagcccagca
1441 tcattcttct cctcttggca gattgtctgt agccgattgg aggagtacaa cagccatcag
1501 tctttatgca atggaacgcc cgagggacct ttacggcgta atcctggaaa ccatgacaaa
1561 tccagaaccc caaggctccc ctcttcagct gatgtagaat tttgcctgag tttgacccaa
1621 tatgaatctg gttccatgga taaagctgcc aatttcagct ttagaaatac actggaagga
1681 tttgctagtc cacttactgg gatagcggat gcctctcaaa gcagcatgca caatgccttg
1741 cacatctata tgaatggaac aatgtcccag gtacagggat ctgccaacga tcctatcttc
1801 cttcttcacc atgcatttgt tgacagtatt tttgagcagt ggctccgaag gcaccgtcct
1861 cttcaagaag tttatccaga agccaatgca cccattggac ataaccggga atcctacatg
1921 gttcctttta taccactgta cagaaatggt gatttcttta tttcatccaa agatctgggc
1981 tatgactata gctatctaca agattcagac ccagactctt ttcaagacta cattaagtcc
2041 tatttggaac aagcgagtcg gatctggtca tggctccttg gggcggcgat ggtaggggcc
2101 gtcctcactg ccctgctggc agggcttgtg agcttgctgt gtcgtcacaa gagaaagcag
2161 cttcctgaag aaaagcagcc actcctcatg gagaaagagg attaccacag cttgtatcag
2221 agccatttat aaaaggctta ggcaatagag tagggccaaa aagcctgacc tcactctaac
2281 tcaaagtaat gtccaggttc ccagagaata tctgctggta ttttctgcag tcgacggtac
2341 cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
2401 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
2461 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
2521 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
2581 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
2641 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
2701 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
2761 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
2821 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
2881 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
2941 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
3001 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
3061 ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca
3121 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
3181 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
3241 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
3301 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
3361 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
3421 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
3481 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
3541 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
3601 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
3661 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
3721 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
3781 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
3841 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
3901 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
3961 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
4021 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
4081 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
4141 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
4201 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
4261 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
4321 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
4381 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
4441 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
4501 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
4561 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
4621 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
4681 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
4741 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
4801 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
4861 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
4921 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
4981 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
5041 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
5101 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
5161 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
5221 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
5281 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
5341 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
5401 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
5461 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
5521 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
5581 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
5641 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
5701 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
5761 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
5821 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
5881 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
5941 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
6001 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
6061 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
6121 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
6181 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
6241 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
6301 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
6361 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
6421 gcat
//
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