| 出品公司: | Addgene |
|---|---|
| 载体名称: | pDR195 |
| 质粒类型: | 酵母菌表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | PMA1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6318bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | URA3 |
| 备注: | -- |
| 产品目录号: | 36028 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | 非病毒 |
买家导航
pDR195载体质粒基本信息
pDR195质粒图谱载体图谱和pDR195载体序列质粒序列多克隆位点信息
pDR195质粒载体简介
pDR195质粒序列载体序列
LOCUS pDR195 6318 bp DNA SYN
DEFINITION pDR195
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6318
/organism="pDR195"
/mol_type="other DNA"
terminator 39..350
/label="yADH2_terminator"
misc_feature complement(43..66)
/label="pJG4_5_rev_primer"
terminator 164..355
/label="yADH1_terminator"
misc_feature complement(219..243)
/label="pHybLex_rev_primer"
misc_feature 360..515
/label="lacZ_a"
promoter complement(363..379)
/label="M13_forward20_primer"
misc_feature complement(372..394)
/label="M13_pUC_fwd_primer"
rep_origin complement(815..1979)
/label="2micron2_origin"
CDS complement(2272..3075)
/label="ORF frame 1"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
gene complement(2275..3075)
/label="URA3"
/gene="URA3"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
CDS 2564..3040
/label="ORF frame 2"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
promoter 3437..3465
/label="AmpR_promoter"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
gene 3507..4367
/label="Ampicillin"
/gene="Ampicillin"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
CDS 3507..4367
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 4522..5141
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 5450..5479
/label="lac_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 5493..5515
/label="M13_pUC_rev_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 5514..5532
/label="M13_reverse_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 cccagcctcg agcggccgcg cggatccagc tttggacttc ttcgccagag gtttggtcaa
61 gtctccaatc aaggttgtcg gcttgtctac cttgccagaa atttacgaaa agatggaaaa
121 gggtcaaatc gttggtagat acgttgttga cacttctaaa taagcgaatt tcttatgatt
181 tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga
241 ctcttaggtt ttaaaacgar aattcttatt cttgagtaac tctttcctgt aggtcaggtt
301 gctttctcag gtatagcatg aggtcgctct tattgaccac acctctaccg gcatgccaat
361 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
421 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
481 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
541 cttacgcatc tgtgcggtat ttcacaccgc ataatcggat cgtacttgtt acccatcatt
601 gaattttgaa catccgaacc tgggagtttt ccctgaaaca gatagtatat ttgaacctgt
661 ataataatat atagtctagc gctttacgga agacaatgta tgtatttcgg ttcctggaga
721 aactattgca tctattgcat aggtaatctt gcacgtcgca tccccggttc attttctgcg
781 tttccatctt gcacttcaat agcatatctt tgttaacgaa gcatctgtgc ttcattttgt
841 agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt
901 tacagaacag aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt
961 tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
1021 atttttacag aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact
1081 tcttttttgt tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag
1141 attacttttt ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg
1201 taggtccgtt aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa
1261 gcctgactcc acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa
1321 gataaaggca tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag
1381 aaagtgatag cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt
1441 tgtctctata tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct
1501 atgaatagtt cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa
1561 aatgtagagg tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata
1621 tagggatata gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc
1681 ggtattcgca atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt
1741 gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagcta
1801 gagaatagga acttcggaat aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa
1861 atgcaacgcg agctgcgcac atacagctca ctgttcacgt cgcacctata tctgcgtgtt
1921 gcctgtatat atatatacat gagaagaacg gcatagtgcg tgtttatgct taaatgcgta
1981 cttatatgcg tctatttatg taggatgaaa ggtagtctag tacctcctgt gatattatcc
2041 cattccatgc ggggtatcgt atgcttcctt cagcactacc ctttagctgt tctatatgct
2101 gccactcctc aattggatta gtctcatcct tcaatgctat catttccttt gatattggat
2161 cgatccgatg ataagctgtc aaacatgaga attgggtaat aactgatata attaaattga
2221 agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg
2281 ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac
2341 cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt cagatcctgt
2401 agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct tgtcatctaa
2461 acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct
2521 ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt
2581 agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca tcaaaaggcc
2641 tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc
2701 caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata cacccgcaga
2761 gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa
2821 attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg aaaaatcagt
2881 caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt caactaactc
2941 cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt gcttttcgtg
3001 catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac gttccttata
3061 tgtagctttc gacatgattt atcttcgttt cctgcatgtt tttgttctgt gcagttgggt
3121 taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat ataccaatct
3181 aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt
3241 tcaaggaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga aaagctaatt
3301 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
3361 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
3421 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
3481 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
3541 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
3601 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
3661 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
3721 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
3781 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
3841 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
3901 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
3961 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
4021 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
4081 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
4141 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
4201 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
4261 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
4321 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
4381 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
4441 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
4501 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
4561 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
4621 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
4681 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
4741 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
4801 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
4861 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
4921 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
4981 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
5041 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
5101 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
5161 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
5221 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
5281 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
5341 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
5401 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
5461 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
5521 gctatgacca tgattacgcc aagcttcctg aaacggagaa acataaacag gcattgctgg
5581 gatcacccat acatcactct gttttgcctg accttttccg gtaatttgaa aacaaacccg
5641 gtctcgaagc ggagatccgg cgataattac cgcagaaata aacccataca cgagacgtag
5701 aaccagccgc acatggccgg agaaactcct gcgagaattt cgtaaactcg cgcgcattgc
5761 atctgtattt cctaatgcgg cacttccagg cctcgatcga gaccgtttat ccattgcttt
5821 tttgttgtct ttttccctcg ttcacagaaa gtctgaagaa gctatagtag aactatgagc
5881 tttttttgtt tctgttttcc tttttttttt ttttacctct gtggaaattg ttactctcac
5941 actctttagt tcgtttgttt gttttgttta ttccaattat gaccggtgac gaaacgtggt
6001 cgatggtggg taccgcttat gctcccctcc attagtttcg attatataaa aaggccaaat
6061 attgtattat tttcaaatgt cctatcatta tcgtctaaca tctaatttct cttaaatttt
6121 ttctctttct ttcctataac accaatagtg aaaatctttt tttcttctat atctacaaaa
6181 actttttttt tctatcaacc tcgttgataa attttttctt taacaatcgt taataattaa
6241 ttaattggaa aataaccatt ttttctctct tttatacaca cattcaaaag aaagaaaaaa
6301 aatatacccc agcctcga
//
