| 质粒名称: | pMXs-Nanog |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 13354 |
| 存储实验室: | Shinya Yamanaka |
|
载体骨架基本信息 |
|
| 载体名称: | pMXs |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4600 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Nanog homeobox,Nanog |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 918 bp |
| 基因突变: | None |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | pMX-S1811 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMXs-Nanog was a gift from Shinya Yamanaka (Addgene plasmid # 13354 ; http://n2t.net/addgene:13354 ; RRID:Addgene_13354) |
| 参考文献部分: | Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi K, Yamanaka S. Cell. 2006 Aug 25. 126(4):663-76. 10.1016/j.cell.2006.07.024 PubMed 16904174 |
买家导航
pMXsNanog载体基本信息
pMXsNanog载体图谱质粒图谱和多克隆位点信息
pMXsNanog载体简介
pMXsNanog载体序列
LOCUS pMXs-Nanog 5733 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Retroviral expression of mouse Nanog.
ACCESSION .
VERSION .
KEYWORDS pMXs-Nanog
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5733)
AUTHORS Takahashi K, Yamanaka S
TITLE Induction of pluripotent stem cells from mouse embryonic and adult
fibroblast cultures by defined factors.
JOURNAL Cell. 2006 Aug 25. 126(4):663-76.
PUBMED 16904174
REFERENCE 2 (bases 1 to 5733)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5733
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(2..21)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(674..1534)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1297..1316
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1535..1639)
/gene="bla"
/label=AmpR promoter
LTR 1669..2262
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 2325..2682
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 2741..3157
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 3087..3109
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 3125..3141
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
misc_feature 3167..3541
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
primer_bind 3479..3495
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
protein_bind 3592..3616
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(4545..4569)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
LTR 4886..5355
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(5484..5501)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5655..510)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
61 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
481 cagattacgc gcagaaaaaa nggatctcaa gaagatcctt tgatcttttc tacggggtct
541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
601 atcttcacct agatcctttt gcggccggcc gcaaatcaat ctaaagtata tatgagtaaa
661 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
721 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
781 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
841 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
901 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
961 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
1021 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
1081 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
1141 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
1201 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
1261 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
1321 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
1381 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
1441 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
1501 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
1561 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
1621 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgcataa tgaaagaccc
1681 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac
1741 ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg
1801 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
1861 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
1921 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca
1981 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
2041 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
2101 acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta
2161 tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc
2221 tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat
2281 cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg gccagcaact
2341 tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct gcgtcggtac
2401 tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga gttcggaaca
2461 cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg tggcccgacc
2521 tgagtccaaa aatcccgatc gttttggact ctttggtgca cccccctaat aggagggata
2581 tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg aatttttgct
2641 ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg ttctgtgttg
2701 tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc
2761 cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt
2821 agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt
2881 cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt
2941 cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc
3001 cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc
3061 tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc
3121 ctccctttat ccagccctca ctccttctct aggcgccccc atatggccat atgagatctt
3181 atatggggca cccccgcccc ttgtaaactt ccctgaccct gacatgacaa gagttactaa
3241 cagcccctct ctccaagctc acttacaggc tctctactta gtccagcacg aagtctggag
3301 acctctggcg gcagcctacc aagaacaact ggaccgaccg gtggtacctc acccttaccg
3361 agtcggcgac acagtgtggg tccgccgaca ccagactaag aacctagaac ctcgctggaa
3421 aggaccttac acagtcctgc tgaccacccc caccgccctc aaagtagacg gcatcgcagc
3481 ttggatacac gccgcccacg tgaaggctgc cgaccccggg ggtggaccat cctctagact
3541 gccggatcta gctagttaat taaggatccc agtgtggtgg tacgggaaat cacaagtttg
3601 tacaaaaaag caggctctga catgagtgtg ggtcttcctg gtccccacag tttgcctagt
3661 tctgaggaag catcgaattc tgggaacgcc tcatcaatgc ctgcagtttt tcatcccgag
3721 aactattctt gcttacaagg gtctgctact gagatgctct gcacagaggc tgcctctcct
3781 cgcccttcct ctgaagacct gcctcttcaa ggcagccctg attcttctac cagtcccaaa
3841 caaaagctct caagtcctga ggctgacaag ggccctgagg aggaggagaa caaggtcctt
3901 gccaggaagc agaagatgcg gactgtgttc tctcaggccc agctgtgtgc actcaaggac
3961 aggtttcaga agcagaagta cctcagcctc cagcagatgc aagaactctc ctccattctg
4021 aacctgagct ataagcaggt taagacctgg tttcaaaacc aaaggatgaa gtgcaagcgg
4081 tggcagaaaa accagtggtt gaagactagc aatggtctga ttcagaaggg ctcagcacca
4141 gtggagtatc ccagcatcca ttgcagctat ccccagggct atctggtgaa cgcatctgga
4201 agcctttcca tgtggggcag ccagacttgg accaacccaa cttggagcag ccagacctgg
4261 accaacccaa cttggaacaa ccagacctgg accaacccaa cttggagcag ccaggcctgg
4321 accgctcagt cctggaacgg ccagccttgg aatgctgctc cgctccataa cttcggggag
4381 gactttctgc agccttacgt acagttgcag caaaacttct ctgccagtga tttggaggtg
4441 aatttggaag ccactaggga aagccatgcg cattttagca ccccacaagc cttggaatta
4501 ttcctgaact actctgtgac tccaccaggt gaaatatgag acttacccag ctttcttgta
4561 caaagtggtg atattccagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca
4621 aaaataataa taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat
4681 gtactattta aactcgagcg gccgccagca cagtggtcga cgataaaata aaagatttta
4741 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct
4801 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga
4861 tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
4921 agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg
4981 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
5041 cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
5101 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
5161 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt
5221 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
5281 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
5341 agcgggggtc tttcacatgc agcatgtatc aaaattaatt tggttttttt cttaagtatt
5401 tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg
5461 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
5521 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
5581 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
5641 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
5701 tcaagtcaga ggtggcgaaa cccgacagga cta
//
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