质粒名称: | pLL39 |
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出品公司: | 爱迪基因 |
目录编号: | 15458 |
存储实验室: | Chia Lee |
载体骨架基本信息 |
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载体名称: | -- |
载体出品公司: | -- |
载体抗性: | 大观霉素 |
空载体大小: | 8019 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 金黄色葡萄球菌 大肠杆菌穿梭载体 |
筛选标记: | -- |
高拷贝/低拷贝: | 低拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | -- |
物种来源: | -- |
插入大小: | 8019 bp |
基因突变: | -- |
GenBank编号 | EF540896 |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pLL39 was a gift from Chia Lee (Addgene plasmid # 15458 ; http://n2t.net/addgene:15458 ; RRID:Addgene_15458) |
参考文献部分: | Improved single-copy integration vectors for Staphylococcus aureus. Luong TT, Lee CY. J Microbiol Methods. 2007 Jul . 70(1):186-90. 10.1016/j.mimet.2007.04.007 PubMed 17512993 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLL39 8019 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pLL39 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8019) AUTHORS Luong TT, Lee CY TITLE Improved single-copy integration vectors for Staphylococcus aureus. JOURNAL J Microbiol Methods. 2007 Jul . 70(1):186-90. PUBMED 17512993 REFERENCE 2 (bases 1 to 8019) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8019 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..57 /label=MCS /note="pUC18/19 multiple cloning site" CDS complement(3285..4076) /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" CDS complement(5328..6278) /codon_start=1 /gene="rep101" /product="RepA protein needed for replication with the pSC101 origin" /label=Rep101 /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI" rep_origin 6326..6548 /label=pSC101 ori /note="low-copy replication origin that requires the Rep101 protein" terminator 7126..7212 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 7309..7395 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 7492..7578 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 7675..7761 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 7858..7944 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttttt 61 tattacctac aacttcttta gcacttaaac gaccatcatc agttattgga acaacgccat 121 aatgcatatg tggtgttttt tcgtccatgt gaactgttgc atataataaa ttatctttac 181 cgtattcttg ttctaaaaac tctttagcat attcaaaaaa ctgctttgta tcttctggcg 241 tttgattatc aaagaaatca ttgtctgatg taattaaacc atcaatgtgt ttaatcgcgt 301 ctgttctaat ttttcttttg cctgtataat tctgttcgat tttttcatca atcaagttat 361 taaaattctg tttattagca tttaccaaat cataatttaa gtaagtttta ctatggtcta 421 tatcttcatt ttcataatta ttattttctc tttgaacatg tttttgtatg cccgttgtat 481 ttgttccaga tttaactttt gaaactctaa caatggaata agacatataa aaatcactcc 541 taagcacaaa ggttttatgt gtatttaata gtttaagtct aacacactag acttattttt 601 aataagtcgt taatacgtgt gctctgcgag gcttaaacct gtttttgcta acgcaaaaaa 661 tgagtggcaa aatgctagcc actcatagtt ctaaaccaaa atataatata actattcaaa 721 ctgcttttca gaacgtttaa atacaataat cgtcaaaaga caacataaaa taataaggat 781 agccatggct acaagaatat tactatacac tccagaagaa taatttataa attccagaac 841 tagtttacga ttaatcaatt gtagtgacaa taaacctcct acaattgcta tacctgttcc 901 ctctgataaa aaacttgtga aatttagcaa actcattcca gaagcaactt cttcttcaga 961 aagactactt gatactattt ttgatataac tgttttagta aaagataatc cgcccataac 1021 aaatataaac ataaaagtag tcaaccacat actaaactca acaaaaaatg caatagttaa 1081 aaaacttata gagatagaca atgatcctaa aataaaaaca aataatgatc cttttctatc 1141 cactaaaaaa ccaccaaaat aaccaaaaac aataacactc atggttccag gaaaaataac 1201 actattacct attgtcgcta catttacatg ataaatagtt ttcatcatat aaggcaccat 1261 tgatataaaa ccagctacta tagaaaatat tagcccacca gaaaacaaac caagcataaa 1321 cggaatgttt ttccctagtt taggattaat aaaagggtta gaaactcttg aaatatgttt 1381 aataaaaatc acaaaaaaga ttgtgaagag tattaaaaaa gtccaattat aatttgtcgt 1441 aaataacata aaacatataa tacttataga cattaaaaca atacctacga tatctaatgt 1501 attttttgtt gatttaccag gtaccattac tttaataaga aaaggtatag ttactattgt 1561 aatcatagga agtataagta ggtaagacca atgaatataa tgtgctatta ttccccctat 1621 tgaaggacct aacccttcac ctaaagctac aattgatcct ataaaaccaa aggctttgcc 1681 ttgttttttt cttgtaatat ttctagctac aaccaccata atcagtgaag ggaatgcagc 1741 agatcctact ccttgtacta acctaccaaa aatcaaaata aaaaagtgat tgtgaccaat 1801 aaaagcaatc aatgaaccaa gacagctcaa actaatacca ataattaaca atttttttat 1861 atttatataa tcagataatt ttccatatac tgctgttcct atcgaaaaag ttaacatata 1921 tgcagtgttt acccagtttg taattccagg agtagtatta aaatgatttg caatatcagg 1981 taaagaaaca tttaaaacca tttcatttaa tacactaaaa aatgaaagaa tacaaagcca 2041 aaataaaacg ctataaaaca aacctttaaa ttttttatat aaactaaaca attttacctc 2101 tttacttgag gtgactaaag tttatagggg taaattattc tttcacctca tataaattcc 2161 ccaaattttt aaatagacac ttcataaaaa aatcctccta aattagaata gtttaaatat 2221 atcatttttg ttcagtaata ttaatatgta cactatttcc aaaatttaaa ttcatgttgc 2281 caaaaatcga ttatgtcttt tgcggggcaa aaaaagggca gattatttga aaaagggcaa 2341 acgcttgtgg aaaagctaaa aggttaaaaa tgacaaaaac cttgatacaa cagtgttttt 2401 ggacgctcgt gtacgttaga gaatgaccgg tttaccatca tacaaggatg ggattaactt 2461 gtgttaaaaa gcctttaata tcagttgtta caaaggattt gtagcgtctt taaaaataaa 2521 aaagggcaga aaaagggcag atacctttta gtacacaagt ttttctaatt tttgctctaa 2581 ctctctgtcc attttctctg ttacatgtgt atacaccttt atagtcgttt tttccagatc 2641 tggaattaat gaggcattct aacattacag aaacaattca aacgtactct catttatata 2701 ctgataaaaa acatcaagct atgagcatat ttgattaaac ggtatcaaat tggtatcaaa 2761 taacaattaa ggagtttata aaatgcgtaa taacaagcct aaaataagta ttcaaaacga 2821 cccatgggaa gtgaaattta tatacattta aatttcatga gacaataaac gttgatttaa 2881 tgcgtttttt tgcctttttt attttcctta ttttttctgt tttacaacaa aatggtatca 2941 aaaatggtat catttgtagt tattttagct tcacatatta aaacaaccac actcctaaat 3001 taataggtgg tgtggttttg ttggttgtgt ggggatcgat agatctggat ctggatttta 3061 atgcggatgt tgcgattact tcgccaacta ttgcgataac aagaaaaagc cagcctttca 3121 tgatatatct cccaatttgt gtagggctta ttatgcacgc ttaaaaataa taaaagcaga 3181 cttgacctga tagtttggct gtgagcaatt atgtgcttag tgcatctaac gcttgagtta 3241 agccgcgccg cgaagcggcg tcggcttgaa cgaattgtta gacattattt gccgactacc 3301 ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc 3361 aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac 3421 tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg 3481 gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc 3541 cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt 3601 tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct 3661 cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct 3721 gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc 3781 cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc 3841 tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca 3901 tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca 3961 tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg 4021 acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatgatg 4081 tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc 4141 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc 4201 ccaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt 4261 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagcttacga 4321 accgaacagg cttatgtcca ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac 4381 ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc 4441 aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag 4501 gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc 4561 ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag 4621 catcgtttgt tcgcccagct tctgtatgga acgggcatgc ggatcagtga gggtttgcaa 4681 ctgcgggtca aggatctgga tttcgatcac ggcacgatca tcgtgcggga gggcaagggc 4741 tccaaggatc gggccttgat gttacccgag agcttggcac ccagcctgcg cgagcagggg 4801 aattaattcc cacgggtttt gctgcccgca aacgggctgt tctggtgttg ctagtttgtt 4861 atcagaatcg cagatccggc ttcagccggt ttgccggctg aaagcgctat ttcttccaga 4921 attgccatga ttttttcccc acgggaggcg tcactggctc ccgtgttgtc ggcagctttg 4981 attcgataag cagcatcgcc tgtttcaggc tgtctatgtg tgactgttga gctgtaacaa 5041 gttgtctcag gtgttcaatt tcatgttcta gttgctttgt tttactggtt tcacctgttc 5101 tattaggtgt tacatgctgt tcatctgtta cattgtcgat ctgttcatgg tgaacagctt 5161 tgaatgcacc aaaaactcgt aaaagctctg atgtatctat cttttttaca ccgttttcat 5221 ctgtgcatat ggacagtttt ccctttgata tgtaacggtg aacagttgtt ctacttttgt 5281 ttgttagtct tgatgcttca ctgatagata caagagccat aagaacctca gatccttccg 5341 tatttagcca gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa 5401 ccattgagat catacttact ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc 5461 tgaatttttg cagttaaagc atcgtgtagt gtttttctta gtccgttatg taggtaggaa 5521 tctgatgtaa tggttgttgg tattttgtca ccattcattt ttatctggtt gttctcaagt 5581 tcggttacga gatccatttg tctatctagt tcaacttgga aaatcaacgt atcagtcggg 5641 cggcctcgct tatcaaccac caatttcata ttgctgtaag tgtttaaatc tttacttatt 5701 ggtttcaaaa cccattggtt aagcctttta aactcatggt agttattttc aagcattaac 5761 atgaacttaa attcatcaag gctaatctct atatttgcct tgtgagtttt cttttgtgtt 5821 agttctttta ataaccactc ataaatcctc atagagtatt tgttttcaaa agacttaaca 5881 tgttccagat tatattttat gaattttttt aactggaaaa gataaggcaa tatctcttca 5941 ctaaaaacta attctaattt ttcgcttgag aacttggcat agtttgtcca ctggaaaatc 6001 tcaaagcctt taaccaaagg attcctgatt tccacagttc tcgtcatcag ctctctggtt 6061 gctttagcta atacaccata agcattttcc ctactgatgt tcatcatctg agcgtattgg 6121 ttataagtga acgataccgt ccgttctttc cttgtagggt tttcaatcgt ggggttgagt 6181 agtgccacac agcataaaat tagcttggtt tcatgctccg ttaagtcata gcgactaatc 6241 gctagttcat ttgctttgaa aacaactaat tcagacatac atctcaattg gtctaggtga 6301 ttttaatcac tataccaatt gagatgggct agtcaatgat aattactagt ccttttcctt 6361 tgagttgtgg gtatctgtaa attctgctag acctttgctg gaaaacttgt aaattctgct 6421 agaccctctg taaattccgg tagacctttg tgtgtttttt ttgtttatat tcaagtggtt 6481 ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc cagccctgtg 6541 tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca aacgctgttt 6601 gctcctctac aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg 6661 ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg cacctgagtc gctgtctttt 6721 tcgtgacatt cagttcgctg cgctcacggc tctggcagtg aatgggggta aatggcacta 6781 caggcgcctt ttatggattc atgcaaggaa actacccata atacaagaaa agcccgtcac 6841 gggcttctca gggcgtttta tggcgggtct gctatgtggt gctatctgac tttttgctgt 6901 tcagcagttc ctgccctctg attttccagt ctgaccactt cggattatcc cgtgacaggt 6961 cattcagact ggctaatgca cccagtaagg cagcggtatc atcaacaggc ttacccgtct 7021 tactgtcggg aattccgatc cccaattcct ggcagtttat ggcgggcgtc ctgcccgcca 7081 ccctccgggc cgttgcttcg caacgttcaa atccgctccc ggcggatttg tcctactcag 7141 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt 7201 tcgttttatt tgatgcctgg ggaattaatt cctggcagtt tatggcgggc gtcctgcccg 7261 ccaccctccg ggccgttgct tcgcaacgtt caaatccgct cccggcggat ttgtcctact 7321 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc 7381 ctttcgtttt atttgatgcc tggggaatta attcctggca gtttatggcg ggcgtcctgc 7441 ccgccaccct ccgggccgtt gcttcgcaac gttcaaatcc gctcccggcg gatttgtcct 7501 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg 7561 agcctttcgt tttatttgat gcctggggaa ttaattcctg gcagtttatg gcgggcgtcc 7621 tgcccgccac cctccgggcc gttgcttcgc aacgttcaaa tccgctcccg gcggatttgt 7681 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga 7741 ctgagccttt cgttttattt gatgcctggg gaattaattc ctggcagttt atggcgggcg 7801 tcctgcccgc caccctccgg gccgttgctt cgcaacgttc aaatccgctc ccggcggatt 7861 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt 7921 cgactgagcc tttcgtttta tttgatgcct ggggaattgg gaattgatcc gctgtaatgg 7981 gcccaatcac tagtgaattc ccgaagctta gatctaatc //