LOCUS pMetYCgate 10021 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Yeast mating-based Split Ubiquitin Assay, bait vector for in vivo
cloning.
ACCESSION .
VERSION .
KEYWORDS pMetYCgate
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10021)
AUTHORS Obrdlik P, El-Bakkoury M, Hamacher T, Cappellaro C, Vilarino C,
Fleischer C, Ellerbrok H, Kamuzinzi R, Ledent V, Blaudez D, Sanders
D, Revuelta JL, Boles E, Andre B, Frommer WB
TITLE K+ channel interactions detected by a genetic system optimized for
systematic studies of membrane protein interactions.
JOURNAL Proc Natl Acad Sci U S A. 2004 Aug 17;101(33):12242-7. Epub 2004 Aug
6.
PUBMED 15299147
REFERENCE 2 (bases 1 to 10021)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10021
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 154..171
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 288..309
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 324..354
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 362..378
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 367..389
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 386..402
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 386..402
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 423..441
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 491..841
/gene="S. cerevisiae MET17"
/gene_synonym="MET15; MET25"
/label=MET17 promoter
/note="expression is repressed by methionine"
protein_bind 842..866
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
gene 953..2309
/label=kanMX
/note="yeast selectable marker conferring kanamycin
resistance (Wach et al., 1994)"
promoter 953..1296
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
CDS 1297..2106
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(1364..1383)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
terminator 2112..2309
/label=TEF terminator
/note="Ashbya gossypii TEF terminator"
protein_bind complement(2363..2387)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 2599..2772
/codon_start=1
/product="IgG-binding unit of Staphylococcus aureus protein
A"
/label=ProtA
/translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
AEAKKLNDAQAPK"
CDS 2776..2946
/codon_start=1
/product="IgG-binding unit of Staphylococcus aureus protein
A"
/label=ProtA
/translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
EAKKLNDAQAPK"
CDS 2965..3570
/codon_start=1
/gene="lexA"
/product="E. coli DNA-binding protein that represses genes
involved in the SOS response to DNA damage"
/label=LexA
/note="The N-terminal DNA-binding domain recognizes the
16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
/translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
primer_bind 3508..3529
/label=LexA
/note="LexA DNA binding domain, forward primer"
CDS 3589..3822
/codon_start=1
/gene="UL48"
/product="transcriptional activation domain of herpes
simplex virus protein VP16 (Triezenberg et al., 1988;
Cousens et al., 1989)"
/label=VP16 AD
/translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
primer_bind complement(3974..3993)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4018..4066
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
primer_bind complement(4368..4387)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(4369..4387)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4394..4411)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(4394..4410)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4403..4425)
/label=M13/pUC Forward
/note="In lacZ gene"
misc_feature 5167..6329
/label=CEN/ARS
/note="S. cerevisiae CEN4 centromere fused to the
autonomously replicating sequence ARS1/ARS416"
rep_origin 5492..6329
/label=ARS1
/note="S. cerevisiae autonomously replicating sequence
ARS1/ARS416"
CDS complement(6691..7785)
/codon_start=1
/gene="S. cerevisiae LEU2"
/product="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
/label=LEU2
/note="yeast auxotrophic marker"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
DLGGSNSTTEVGDAVAEEVKKILA"
promoter complement(7786..8191)
/gene="S. cerevisiae LEU2"
/label=LEU2 promoter
primer_bind complement(8211..8229)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 8297..8401
/gene="bla"
/label=AmpR promoter
CDS 8402..9262
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8620..8639)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9433..10021
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9922..9941
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
421 gaaattaacc ctcactaaag ggaacaaaag ctgggtaccg gatgcaaggg ttcgaatccc
481 ttagctctca ttattttttg ctttttctct tgaggtcaca tgatcgcaaa atggcaaatg
541 gcacgtgaag ctgtcgatat tggggaactg tggtggttgg caaatgacta attaagttag
601 tcaaggcgcc atcctcatga aaactgtgta acataataac cgaagtgtcg aaaaggtggc
661 accttgtcca attgaacacg ctcgatgaaa aaaataagat atatataagg ttaagtaaag
721 cgtctgttag aaaggaagtt tttccttttt cttgctctct tgtcttttca tctactattt
781 ccttcgtgta atacagggtc gtcagataca tagatacaat tctattaccc ccatccatac
841 aacaagtttg tacaaaaaag caggctctcc aaccaccatg aattctatac gaagttatta
901 ggtctagaga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga
961 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
1021 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
1081 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa
1141 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
1201 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg
1261 atacagttct cacatcacat ccgaacataa acaaccatgg gtaaggaaaa gactcacgtt
1321 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
1381 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca
1441 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
1501 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
1561 cctgatgatg catggttact caccactgcg atccccggca aaacagcatt ccaggtatta
1621 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
1681 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
1741 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
1801 aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg
1861 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
1921 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
1981 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
2041 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
2101 ttctaatcag tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt
2161 ttttttatat tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc
2221 gcctcgacat catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca
2281 atcgtatgtg aatgctggtc gctatactgc tgtcgattcg atactaacgc cgccatccag
2341 tgtcgaaaac gagctcccgg gtacccagct ttcttgtaca aagtggttgg tggtggcgga
2401 tccggtggag gtggatcagg tatccctcca gatcaacaaa gattgatctt tgccggtaag
2461 cagctagaag acggtagaac gctgtctgat tacaacattc agaaggagtc caccttacat
2521 cttgtgctaa ggctaagagg tggtatgcac agatcagctt gcggccgcat ggcaggcctt
2581 gcgcaacacg atgaagccgt ggacaacaaa ttcaacaaag aacaacaaaa cgcgttctat
2641 gagatcttac atttacctaa cttaaacgaa gaacaacgaa acgccttcat ccaaagttta
2701 aaagatgacc caagccaaag cgctaacctt ttagcagaag ctaaaaagct aaatgatgct
2761 caggcgccga aagtagacaa caaattcaac aaagaacaac aaaacgcgtt ctatgagatc
2821 ttacatttac ctaacttaaa cgaagaacaa cgaaacgcct tcatccaaag tttaaaagat
2881 gacccaagcc aaagcgctaa ccttttagca gaagctaaaa agctaaatga tgctcaggcg
2941 ccgaaagtag acgcgagcgg ccgcatgaaa gcgttaacgg ccaggcaaca agaggtgttt
3001 gatctcatcc gtgatcacat cagccagaca ggtatgccgc cgacgcgtgc ggaaatcgcg
3061 cagcgtttgg ggttccgttc cccaaacgcg gctgaagaac atctgaaggc gctggcacgc
3121 aaaggcgtta ttgaaattgt ttccggcgca tcacgcggga tccgtctgtt gcaggaagag
3181 gaagaagggt tgccgctggt aggtcgtgtg gctgccggtg aaccacttct ggcgcaacag
3241 catattgaag gtcattatca ggtcgaccct tccttattca agccgaatgc tgatttcctg
3301 ctgcgcgtca gcgggatgtc gatgaaagat atcggcatta tggatggtga cttgctggca
3361 gtgcataaaa ctcaggatgt acgtaacggt caggtcgttg tcgcacgtat tgatgacgag
3421 gttaccgtta agcgcctgaa aaaacagggc aataaagtcg aactgttgcc agaaaatagc
3481 gagtttaaac caattgtcgt agatcttcgt cagcagagct tcaccattga agggctggcg
3541 gttggggtta ttcgcaacgg cgactggctg gaattcccgg ggatctgggc ccccccgacc
3601 gatgtcagcc tgggggacga gctccactta gacggcgagg acgtggcgat ggcgcatgcc
3661 gacgcgctag acgatttcga tctggacatg ttgggggacg gggattcccc gggtccggga
3721 tttacccccc acgactccgc cccctacggc gctctggata tggccgactt cgagtttgag
3781 cagatgttta ccgatgccct tggaattgac gagtacggtg ggtagggggc gcgaccggac
3841 ccgcatcccc cgtctgggtt ttcccctccc gtccccggtt cgtatccaca ataaacacga
3901 gcacatacat tacaaaaacc tgcggttgtc gtctgattat ttggtggatc cgggggagat
3961 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
4021 caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt
4081 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
4141 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
4201 gccaacgtcg gggcggcagg ccctgccata gccactggcc ccgtgggtta gggacggggt
4261 cccccatggg gaatggttta tggttcgtgg gggttattat tttgggcgtt gcgtggggtc
4321 aggtccacga cccaagctgg ccgccaccgc ggtggagctc caattcgccc tatagtgagt
4381 cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
4441 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
4501 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
4561 ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataatcgct gggccattct
4621 catgaagaat atcttgaatt tattgtcata ttactagttg gtgtggaagt cctaatatcg
4681 gtgatcaata tagtggttga catgctggct agtcaacatt gagccttttg atcatgcaaa
4741 tatattacgg tattttacaa tcaaatatca aacttaacta ttgactttat aacttattta
4801 ggtggtaaca ttcttataaa aaagaaaaaa attactgcaa aacagtacta gcttttaact
4861 tgtatcctag gttatctatg ctgtctcacc atagagaata ttacctattt cagaatgtat
4921 gtccatgatt cgccgggtaa atacatataa tacacaaatc tggcttaata aagtctataa
4981 tatatctcat aaagaagtgc taaattggct agtgctatat atttttaaga aaatttcttt
5041 tgactaagtc catatcgact ttgtaaaagt tcacattagc atacatatat tacacgagcc
5101 agaaatagta acttttgcct aaatcacaaa ttgcaaaatt taattgcttg caaaaggtca
5161 catgcttata atcaactttt ttaaaaattt aaaatacttt tttatttttt atttttaaac
5221 ataaatgaaa taatttattt attgtttatg attaccgaaa cataaaacct gctcaagaaa
5281 aagaaactgt tttgtccttg gaaaaaaagc actacctagg agcggccaaa atgccgaggc
5341 tttcatagct taaactcttt acagaaaata ggcattatag atcagttcga gttttcttat
5401 tcttccttcc ggttttatcg tcacagtttt acagtaaata agtatcacct cttagagttc
5461 gatgataagc tgtcaaacat gagaattaat tccacatgtt aaaatagtga aggagcatgt
5521 tcggcacaca gtggaccgaa cgtggggtaa gtgcactagg gtccggttaa acggatctcg
5581 cattgatgag gcaacgctaa ttatcaacat atagattgtt atctatctgc atgaacacga
5641 aatctttact tgacgacttg aggctgatgg tgtttatgca aagaaaccac tgtgtttaat
5701 atgtgtcact gtttgatatt actgtcagcg tagaagataa tagtaaaagc ggttaataag
5761 tgtatttgag ataagtgtga taaagttttt acagcgaaaa gacgataaat acaagaaaat
5821 gattacgagg atacggagag aggtatgtac atgtgtattt atatactaag ctgccggcgg
5881 ttgtttgcaa gaccgagaaa aggctagcaa gaatcgggtc attgtagcgt atgcgcctgt
5941 gaacattctc ttcaacaagt ttgattccat tgcggtgaaa tggtaaaagt caaccccctg
6001 cgatgtatat tttcctgtac aatcaatcaa aaagccaaat gatttagcat tatctttaca
6061 tcttgttatt ttacagattt tatgtttaga tcttttatgc ttgcttttca aaaggcttgc
6121 aggcaagtgc acaaacaata cttaaataaa tactactcag taataaccta tttcttagca
6181 tttttgacga aatttgctat tttgttagag tcttttacac catttgtctc cacacctccg
6241 cttacatcaa caccaataac gccatttaat ctaagcgcat caccaacatt ttctggcgtc
6301 agtccaccag ctaacataaa atgtaagctc tcggggctct cttgccttcc aacccagtca
6361 gaaatcgagt tccaatccaa aagttcacct gtcccacctg cttctgaatc aaacaaggga
6421 ataaacgaat gaggtttctg tgaagctgca ctgagtagta tgttgcagtc ttttggaaat
6481 acgagtcttt taataactgg caaaccgagg aactcttggt attcttgcca cgactcatct
6541 ccatgcagtt ggacgatcga tgataagctg tcaaacatga gaattaattc taccctatga
6601 acatattcca ttttgtaatt tcgtgtcgtt tctattatga atttcattta taaagtttat
6661 gtacaaatat cataaaaaaa gagaatcttt ttaagcaagg attttcttaa cttcttcggc
6721 gacagcatca ccgacttcgg tggtactgtt ggaaccacct aaatcaccag ttctgatacc
6781 tgcatccaaa acctttttaa ctgcatcttc aatggcctta ccttcttcag gcaagttcaa
6841 tgacaatttc aacatcattg cagcagacaa gatagtggcg atagggttga ccttattctt
6901 tggcaaatct ggagcagaac cgtggcatgg ttcgtacaaa ccaaatgcgg tgttcttgtc
6961 tggcaaagag gccaaggacg cagatggcaa caaacccaag gaacctggga taacggaggc
7021 ttcatcggag atgatatcac caaacatgtt gctggtgatt ataataccat ttaggtgggt
7081 tgggttctta actaggatca tggcggcaga atcaatcaat tgatgttgaa ccttcaatgt
7141 aggaaattcg ttcttgatgg tttcctccac agtttttctc cataatcttg aagaggccaa
7201 aacattagct ttatccaagg accaaatagg caatggtggc tcatgttgta gggccatgaa
7261 agcggccatt cttgtgattc tttgcacttc tggaacggtg tattgttcac tatcccaagc
7321 gacaccatca ccatcgtctt cctttctctt accaaagtaa atacctccca ctaattctct
7381 gacaacaacg aagtcagtac ctttagcaaa ttgtggcttg attggagata agtctaaaag
7441 agagtcggat gcaaagttac atggtcttaa gttggcgtac aattgaagtt ctttacggat
7501 ttttagtaaa ccttgttcag gtctaacact acctgtaccc catttaggac cacccacagc
7561 acctaacaaa acggcatcag ccttcttgga ggcttccagc gcctcatctg gaagtggaac
7621 acctgtagca tcgatagcag caccaccaat taaatgattt tcgaaatcga acttgacatt
7681 ggaacgaaca tcagaaatag ctttaagaac cttaatggct tcggctgtga tttcttgacc
7741 aacgtggtca cctggcaaaa cgacgatctt cttaggggca gacattagaa tggtatatcc
7801 ttgaaatata tatatatatt gctgaaatgt aaaaggtaag aaaagttaga aagtaagacg
7861 attgctaacc acctattgga aaaaacaata ggtccttaaa taatattgtc aacttcaagt
7921 attgtgatgc aagcatttag tcatgaacgc ttctctattc tatatgaaaa gccggttccg
7981 gcgctctcac ctttcctttt tctcccaatt tttcagttga aaaaggtata tgcgtcaggc
8041 gacctctgaa attaacaaaa aatttccagt catcgaattt gattctgtgc gatagcgccc
8101 ctgtgtgttc tcgttatgtt gaggaaaaaa ataatggttg ctaagagatt cgaactcttg
8161 catcttacga tacctgagta ttcccacagt taattcttga agacgaaagg gcctcgtgat
8221 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
8281 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
8341 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
8401 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
8461 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
8521 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
8581 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
8641 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
8701 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
8761 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
8821 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
8881 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
8941 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
9001 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
9061 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
9121 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
9181 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
9241 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
9301 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
9361 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
9421 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
9481 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
9541 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
9601 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
9661 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
9721 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
9781 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
9841 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
9901 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
9961 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
10021 a
//
