LOCUS pRADZ1 9988 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION General cloning vector for use in E. coli or D. radiodurans with
lacZ.
ACCESSION .
VERSION .
KEYWORDS pRADZ1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9988)
AUTHORS Meima R, Lidstrom ME
TITLE Characterization of the minimal replicon of a cryptic Deinococcus
radiodurans SARK plasmid and development of versatile Escherichia
coli-D. radiodurans shuttle vectors.
JOURNAL Appl Environ Microbiol. 2000 Sep;66(9):3856-67.
PUBMED 10966401
REFERENCE 2 (bases 1 to 9988)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9988
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 27..131
/gene="bla"
/label=AmpR promoter
CDS 132..992
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(350..369)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1163..1751
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1652..1671
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1905..1922
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2039..2060
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2075..2105
/label=lac promoter
/note="promoter for the E. coli lac operon"
primer_bind 2117..2139
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2136..2152
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2136..2152
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 9144..9803
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(9210..9229)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
2101 tgttgtgtgg aattggagcg gataacaatt tcacacagga aacagctatg accatgatta
2161 cgccaagctc gcgaggcctc gagatctata gggaaaaggt ggtgaactac tgtggaagtt
2221 actgacgtaa gattacgggt cgaccgggaa aaccctggcg ttacccaact taatcgcctt
2281 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
2341 tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa
2401 gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc
2461 tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt
2521 acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt
2581 aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac
2641 tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg
2701 ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg
2761 gtgctgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc
2821 attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt
2881 gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc
2941 ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc
3001 gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat
3061 cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat
3121 ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc
3181 tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag
3241 ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg
3301 gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg
3361 cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg
3421 tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc
3481 gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat
3541 cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat
3601 cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa
3661 ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat
3721 gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct
3781 ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc
3841 ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc
3901 tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct
3961 tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc
4021 tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc
4081 cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc
4141 gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa
4201 gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag
4261 ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca
4321 tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt
4381 gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt
4441 tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag
4501 atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca
4561 ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc
4621 gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca
4681 gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa
4741 accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc
4801 gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag
4861 ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc
4921 gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc
4981 ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc
5041 ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg
5101 atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac
5161 ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaatta
5221 cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata ataataaccg
5281 ggcaggccat gtctgcccgt atttcgcgta aggaaatcca ttatgtacta tttcaagcta
5341 attccggtgg aagccgaatt ctgcagatat ccatcacact ggcggccgct cgagcatgca
5401 tctagaagct tctgcagacg cgtcgacgtc atatggatcc gattcgacct gcaggcatgc
5461 aagctgtacg cctcagctac cattcttgca gccgcgttca gggtcttgac gtgttctttg
5521 tatctatcag caaactgaga acccaattct ggatcatcct tgtaacgcca actctccgac
5581 cattccgaag gaacgccttg tagtcctcct gagccgttag ggtatgtctt tagtccaatc
5641 aaatgggaca tttgcagaca ggaacgcaca agtgtctcac gtcgcttctc tatttcaata
5701 tctaaaaatt cttcactctt atcgttgctt gacatattaa aactgtctaa ctgatccagt
5761 tcacttatat ggaaagtatc actaaagtca tggcttctca gaaattcaat acttcccatt
5821 gatggggacc ggttaaccaa atcgataaaa atttttctgt catgtttcag ataagttttt
5881 ggcgcgttga ttttataata aagccgccat tcttgtgcca acaacgccac gaaaagagcc
5941 acagcagcag cggccacatc ccattgaaaa tctttcgcca gaccaaccgc caggagaacc
6001 aagccgaaat agttagccca gcgaactaaa aaactatcta taaacgtatt aagaaacgtt
6061 cgggtcatat ttccgtgaat catatcagga cgtaaatttg cacatgacgc atttccgggg
6121 gcttttatcc ctcggacttc ttttcaggcc ccttctcggc ctttctgtga ccctgttttt
6181 tcaggccgcg aactgcgcct ggtcgggcct gacccccaca cgctggcctt cgtactcacg
6241 cagcctgccc agcaggtcgg ccagcgcggc attgaccacg gcggcgaggt tgcggggcgg
6301 cgttccgcct gcggtctggt cgtgcttcac gtcgcgcagc acgcgcacca gcaccgcgcc
6361 cacgtcgtcg gacacgtccc ggccagcgtc ggcggcgcgg gtgatgttcc aaatgagttt
6421 ccgccagaag cccagcgagt cggccccgtc cccgaaagct gccgctagcg cccgcgcctg
6481 cctgtccacg atctcggcgc ggtaggccgg gcgggtttcc cggtgggcgt cggcaagctc
6541 ccacacgacg ttttcagcga gggagggcgc gggccggaca gtcatgttat cggaggggga
6601 gggaagagcg gattttatcg cccaggtgct cacaacttcg tatgtcacgg caaccggggc
6661 gcttttttcg cgcagttccc cgcctgtcac atcctcacag ggttcttgac tctccggcac
6721 gctcttaggg agagggtgga gcatgttgta gacggtgcgc ccggccttcg cgtcggcgtt
6781 caggtcgcgc cagttccggc cccaatcgtc atgcatcagg cggacatatc ccgccttccc
6841 ttccagcacg cgcctcggct tgagcgtcac ggcccacagc gtcccggttg ccacagactc
6901 gccgcgcagg tcgcccatgt gggcatcaca ggccaccagc ccgacccggc gcagatattg
6961 aaggttctcg tagaaggcgg acttcttcag acccacatgc accatgagca gctcggcggg
7021 caggtggaac acagcgcggg tgacgtgttc cgcatagccg caggcgcggg ccacgtccag
7081 cgccaccgag aacagggcgc ggaagatgcg ccgggccgac tcgcggcagg gggcgtcatc
7141 taggcaggcg gtgagggtct gcaccagttc ggcgcgtgtg gcgatggtca acggcgcggg
7201 cgcgggctgg gactgctggg cgatggtggg cacgggcggc ggcgagatgg accgggccga
7261 gctggaaatt tcccgtgtga gcgcaggttt aggcgcatgg ttaccttcct gcagtgaaat
7321 ctgcgaaatc aggcccgctt cgaggagtcg gaccatgaat ggattgtgtc tcaagaaaaa
7381 agcctcccct cttgtcagag gggagggtcc cggtctacca tgctctcagc gacgagatag
7441 ctggggtttg tgaccctgca ctctgacaaa gccctcaccg aaaggtgggg gtttagtcat
7501 ttggcctagt aaagcagaac agaggcgatt tgtcacgcaa aggcccgccg cccgagggga
7561 aacaggcggc gggccaatgg ctcggcgttg ttagctgggg gtcgcaccag ggaagcacag
7621 aagcccttcc ctgagcaggg tttcaccgtg tggtgtccct tcgtccttga aggcgaactg
7681 gtaggccagg ccgtaatgct cctgcaccat cttttgctca tccaccgcga caaagaaacg
7741 gcgggtgttc gccgggctgc tgagggtctg ccactcgccg gacgcgccca ccgcataggc
7801 gtctccggct ccacattcct cggcctgctg atactgatac ttcgtccaca gcatcaccgc
7861 cttatcgggc acctgcgcga agctcaccag cgccccgaac cgcccggcgc tcgtggtgaa
7921 ggtggacgac tgcaccatgt cggacagttc cagcgtggca ggcgcgggcg tgttcgtcgg
7981 cgcgtacacc gtccccgcct cgcacacgac cagcggggcg gcccccgtgc cgaactcggc
8041 ggcgctgccg tttgccagcg tgagcgccga agacgccacc gcgcaggtac ccatgggtgc
8101 cgaccaccag caggtccacg ccgtgcgccg cctcggtgag cacctcgatt ggacggcccc
8161 tcagggtctg aaccctcccc tgcacgcccg cctccccgat gagtgtctgc gccgtctgct
8221 gcgcctgccg ggcattctgc tccagcatct cccggacgta gtcgggattc agtcccgccc
8281 actgaacgaa gccgctgacc ggcggctcct cgatcacagt cacgacctcc agctcggcgc
8341 ctgccgcctt cccgagcgtt atggcctgct tcaaggcgcg ctggctgggc tcacttccgt
8401 caaaggccac cacgaatcgc atccgtcctc ctccctgacc agtcaggttc agcaagcagg
8461 gtaagcctct cccagcactc cggggcttca cacggccttg ggtcttttct gcatacccat
8521 gagcctcagt acccgacagt ttccggcagg gcttgaaaaa gttcggctga cacgcgagaa
8581 ctggacgcgt ctcccgatgt cctggccaag ttgacccgct gcttcaccgc gcacttcccg
8641 gagttccgca agaactaggt cgagctgctc tccctcatgg tcctcgccct ccttaggggc
8701 aaggacgtcc ggcatgctaa actcgccgcg cgcttccccg gaagcgcgca caccgcctcc
8761 gtcatccggc gggtggaacg cttcttcgac cgtcatcctc ttcggccagc tgatgtcgcc
8821 cgggtcgttc tgacgctcct tcccgccgcg cagccacgcg aatttatcct tgaccggacc
8881 aactggaagt atgggcagac ggacgtgaac gtcttgttgc tggccgtcat ttggcgggac
8941 gtcgccatcc ccctgctcta cgagttgctg ccccatgggg gcagcagcga caccgagatt
9001 cggcacaccc tgatggacga tgccctgtgc ctgctgtccg ccgctgacat ccgggtgctg
9061 tattagccga ccgcgaattc ccagcttgag taggacaaat ccgccgagct tcgacgagat
9121 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
9181 tatcccaatc gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
9241 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
9301 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
9361 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
9421 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
9481 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
9541 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
9601 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
9661 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
9721 atgccgtctg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
9781 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
9841 gtgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcgt cgaggcggca
9901 cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt
9961 gaatgcatcc cgcaagaggc ccggcagt
//
