出品公司: | Invitrogen |
---|---|
载体名称: | pTracer-CMV2,pTracerCMV2 |
质粒类型: | 哺乳动物细胞载体 |
复制子: | pUC |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 6210bp |
5' 测序引物及序列: | CMV-F(CGCAAATGGGCGGTAGGCGTG) |
3' 测序引物及序列: | BGH(TAGAAGGCACAGTCGAGG) |
终止子: | bGH poly(A) signal |
载体抗性: | 氨苄青霉素 |
筛选标记: | Zeo |
克隆菌株: | DH5α 等 |
宿主细胞(系): | 293T等哺乳细胞 |
培养条件: | 37℃,5%CO2 |
产品目录号: | -- |
稳定性: | -- |
组成型/诱导型: | 无需诱导 |
病毒/非病毒: | -- |
买家导航
pTracer-CMV2载体 pTracerCMV2质粒基本信息
pTracer-CMV2质粒图谱 pTracerCMV2载体图谱和pTracer-CMV2载体序列 pTracerCMV2质粒序列多克隆位点信息
pTracer-CMV2质粒 pTracerCMV2载体简介
pTracer-CMV2的CMV启动子后有MCS供插入外源基因,hEF1α启动子调控Cycle 3 GFP-Zeocin fusion的表达,可以做到既有荧光蛋白来做转染指示,且荧光基因表达和外源基因表达又不会互相干扰,而且Zeocin也可以用来做稳定性细胞筛选。另外,由于EM-7启动子的存在,Cycle 3 GFP-Zeocin fusion在E.coli中也可以表达,可以用来做原核荧光定位。
pTracer-CMV2质粒序列 pTracerCMV2载体序列
LOCUS pTracer-CMV2 6210 bp ds-DNA circular SYN 04-11-2015 KEYWORDS Untitled 4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6210) AUTHORS admin TITLE Direct Submission JOURNAL Exported 2015-11-4 from MiaoLingPlasmid http://www.miaolingbio.com FEATURES Location/Qualifiers source 1..6210 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 235..614 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" promoter 863..881 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 1028..1139 /note="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" promoter 1309..2487 /note="EF-1-alpha promoter" /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 1540..2478 /note="EF-1-alpha intron A" /note="intron upstream of the start codon of human EF-1-alpha" promoter 2504..2522 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" promoter 2611..2658 /note="EM7 promoter" /note="synthetic bacterial promoter " CDS 3376..3753 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /note="BleoR" /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MDAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWG REFALRDPAGNCVHFVAEEQD" polyA_signal 3883..4004 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" primer_bind complement(4053..4069) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 4077..4093 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4101..4131) /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 4146..4167 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(4455..5043) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5214..6074) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(6075..6179) /gene="bla" /note="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc 961 agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca 1021 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 1081 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 1141 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 1201 gaggattggg aagacaatag cagctttgca aagatggata aagttttaaa cagagaggaa 1261 tctttgcagc taatggacct tctaggtctt gaaaggagtg cctcgtgagg ctccggtgcc 1321 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 1381 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 1441 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 1501 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc 1561 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg 1621 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg 1681 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg 1741 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct 1801 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt 1861 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac 1921 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg 1981 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg 2041 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg 2101 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg 2161 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc 2221 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc 2281 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac 2341 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa 2401 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt 2461 ttttttcttc catttcaggt gtcgtgagga attagcttgg tactaatacg actcactata 2521 gggagaccca agctggctag gtaagctcct aggcttttgc aaaaagctcc cgggagcttg 2581 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc 2641 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ctagcaaagg agaagaactt 2701 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt 2761 tctgtcagtg gagagggtga aggtgatgct acatacggaa agcttaccct taaatttatt 2821 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt ctcttatggt 2881 gttcaatgct tttcccgtta tccggatcat atgaaacggc atgacttttt caagagtgcc 2941 atgcccgaag gttatgtaca ggaacgcact atatctttca aagatgacgg gaactacaag 3001 acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta atcgtatcga gttaaaaggt 3061 attgatttta aagaagatgg aaacattctc ggacacaaac tcgagtacaa ctataactca 3121 cacaatgtat acatcacggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt 3181 cgccacaaca ttgaagatgg atccgttcaa ctagcagacc attatcaaca aaatactcca 3241 attggcgatg gccctgtcct tttaccagac aaccattacc tgtcgacaca atctgccctt 3301 tcgaaagatc ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt aactgctgct 3361 gggattacac atggcatgga tgccaagttg accagtgccg ttccggtgct caccgcgcgc 3421 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg 3481 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag 3541 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg 3601 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg 3661 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac 3721 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc 3781 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 3841 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct 3901 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 3961 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg 4021 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 4081 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 4141 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 4201 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 4261 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 4321 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 4381 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 4441 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 4501 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 4561 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 4621 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg 4681 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 4741 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 4801 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 4861 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 4921 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 4981 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 5041 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 5101 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 5161 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 5221 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 5281 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 5341 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 5401 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 5461 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 5521 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 5581 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 5641 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 5701 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 5761 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 5821 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 5881 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 5941 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 6001 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 6061 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 6121 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 6181 acatttcccc gaaaagtgcc acctgacgtc //
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pTracer-CMV2质粒 pTracerCMV2载体应用举例
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