| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pTracer-CMV2,pTracerCMV2 |
| 质粒类型: | 哺乳动物细胞载体 |
| 复制子: | pUC |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 6210bp |
| 5' 测序引物及序列: | CMV-F（CGCAAATGGGCGGTAGGCGTG） |
| 3' 测序引物及序列: | BGH（TAGAAGGCACAGTCGAGG） |
| 终止子: | bGH poly(A) signal |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Zeo |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 293T等哺乳细胞 |
| 培养条件: | 37℃，5%CO2 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | 无需诱导 |
| 病毒/非病毒: | -- |
买家导航
pTracerCMV2载体基本信息
pTracerCMV2载体图谱质粒图谱和多克隆位点信息
pTracerCMV2载体简介
pTracer-CMV2的CMV启动子后有MCS供插入外源基因，hEF1α启动子调控Cycle 3 GFP-Zeocin fusion的表达，可以做到既有荧光蛋白来做转染指示，且荧光基因表达和外源基因表达又不会互相干扰，而且Zeocin也可以用来做稳定性细胞筛选。另外，由于EM-7启动子的存在，Cycle 3 GFP-Zeocin fusion在E.coli中也可以表达，可以用来做原核荧光定位。
pTracerCMV2载体序列
LOCUS pTracer-CMV2 6210 bp ds-DNA circular SYN 04-11-2015
KEYWORDS Untitled 4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6210)
AUTHORS admin
TITLE Direct Submission
JOURNAL Exported 2015-11-4 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..6210
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 1028..1139
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
promoter 1309..2487
/note="EF-1-alpha promoter"
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 1540..2478
/note="EF-1-alpha intron A"
/note="intron upstream of the start codon of human
EF-1-alpha"
promoter 2504..2522
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 2611..2658
/note="EM7 promoter"
/note="synthetic bacterial promoter "
CDS 3376..3753
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="BleoR"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MDAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWG
REFALRDPAGNCVHFVAEEQD"
polyA_signal 3883..4004
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(4053..4069)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 4077..4093
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4101..4131)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 4146..4167
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4455..5043)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5214..6074)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6075..6179)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
961 agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca
1021 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
1081 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
1141 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
1201 gaggattggg aagacaatag cagctttgca aagatggata aagttttaaa cagagaggaa
1261 tctttgcagc taatggacct tctaggtctt gaaaggagtg cctcgtgagg ctccggtgcc
1321 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
1381 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
1441 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
1501 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc
1561 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg
1621 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg
1681 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
1741 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
1801 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
1861 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac
1921 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
1981 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
2041 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
2101 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
2161 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
2221 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
2281 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
2341 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
2401 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
2461 ttttttcttc catttcaggt gtcgtgagga attagcttgg tactaatacg actcactata
2521 gggagaccca agctggctag gtaagctcct aggcttttgc aaaaagctcc cgggagcttg
2581 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
2641 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ctagcaaagg agaagaactt
2701 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
2761 tctgtcagtg gagagggtga aggtgatgct acatacggaa agcttaccct taaatttatt
2821 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt ctcttatggt
2881 gttcaatgct tttcccgtta tccggatcat atgaaacggc atgacttttt caagagtgcc
2941 atgcccgaag gttatgtaca ggaacgcact atatctttca aagatgacgg gaactacaag
3001 acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta atcgtatcga gttaaaaggt
3061 attgatttta aagaagatgg aaacattctc ggacacaaac tcgagtacaa ctataactca
3121 cacaatgtat acatcacggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt
3181 cgccacaaca ttgaagatgg atccgttcaa ctagcagacc attatcaaca aaatactcca
3241 attggcgatg gccctgtcct tttaccagac aaccattacc tgtcgacaca atctgccctt
3301 tcgaaagatc ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt aactgctgct
3361 gggattacac atggcatgga tgccaagttg accagtgccg ttccggtgct caccgcgcgc
3421 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
3481 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
3541 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
3601 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
3661 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
3721 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
3781 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
3841 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
3901 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
3961 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
4021 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
4081 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
4141 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
4201 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
4261 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
4321 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
4381 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
4441 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
4501 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
4561 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
4621 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
4681 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
4741 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
4801 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
4861 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
4921 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
4981 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
5041 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
5101 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
5161 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
5221 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
5281 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
5341 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
5401 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
5461 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
5521 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
5581 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
5641 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
5701 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
5761 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
5821 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
5881 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
5941 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
6001 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
6061 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
6121 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
6181 acatttcccc gaaaagtgcc acctgacgtc
//
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