pTRV2-GFP

LOCUS       pTRV2-GFP               11720 bp DNA     circular SYN 10-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 10, 2024 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(48..65)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(1054..1773)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(2943..3288)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    3817..3841
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4266..5060
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6485..7073
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7259..7399
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      7743..7937
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8003..9076)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9505..10134)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11435..11459
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(join(11481..11720,1..13))
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 tgtttgaacg atcggggaaa ttcgagctgg tcacctgtaa ttcacacgtg gtggtggtgg
       61 tggtggctag cgttctcccg tttcgtcctt tagggactcg tcagtgtact gatataagta
      121 cagacgggcg taataacgct tacgtaggcg aggggtttta ccccttttgc ctttgtaacc
      181 atcatcactt taaaagtact tactcacccc ccaacaatct cttagccgcg aggctttttc
      241 agatcgttga gaattttaat aaaatacaaa taaaataaca acacaaacaa agtccgttcc
      301 cctatggtaa gacaatgagt cggccaaacg ccgatctcaa acagtctata cacagaaaca
      361 gataacaatt taaaataaaa tcaaaaagaa aattaaatta caaaagactt accgatcaat
      421 caagatcagt cgagaatgtc aatctcgtag gtttaaattg aacctaaaac ttcagacacg
      481 gatctactta aagaaccgta gtttaatgtc ttcgggacat gcccgggcct cgagacgcgt
      541 gagctcggta ccggatcccc atggaggcct tctagagaat tcggtaacct tactcacaga
      601 atctaagtcc actcgtccgt aataataaag ctcatcgtgc ttccttgagt aacaatgtcc
      661 atctttgttg acacacaaga accgcctgaa cataaaaaca aatacgttct ctacttttcc
      721 ctcaaacagc gtatcatctc ccagtgaatt atcaaaaact gacaactttc caattacttc
      781 atacgtgcga atatgtggga ctctactacg taccttgacc accaaaccat tatctaacaa
      841 taacacatta gtgtcgtcaa gccacttcct aagtaattcg tgcattgcga aactcaaatg
      901 ctaccaacga cactgacgtc aatggtaaag gaaattccct caaattagtc acaattagcc
      961 ctatttagat gtcgctaatg gcatcagtga atatggtatc acccaccctc tgaatttcac
     1021 agagatctca acagagttca cgtccttaaa tccttacttg tacagctcgt ccatgccgag
     1081 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
     1141 gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc
     1201 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat
     1261 gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata
     1321 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
     1381 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
     1441 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
     1501 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa
     1561 gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
     1621 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
     1681 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
     1741 cccggtgaac agctcctcgc ccttgctcac catgggatta ggacgtatcg gacttccact
     1801 cgctggaggc ggtgcagatg aactagcagc tgctccacca aacttaatcc cgaatacttt
     1861 ctcaaagttc cttcggtaat aactcttcaa ctccatgttc tctaacgaag ttagagattt
     1921 gtggaaagct gtagaggcat catcgaattt gtaataagtg tacaagtctt cttccaaagt
     1981 cgagccagtg ttcgccttgg tagtagtagt actcccttgg ttcgtcgtaa ccgttgtgtt
     2041 tggattcgca ggaatatttg taacacccgc cactgcattc ttctcatcag ctcgtctctt
     2101 gaacgctgaa gcgttctgaa tcacaccaag agcactgtta gcccgtgagt ctaaaaggaa
     2161 atacaccttc ttatctggaa actgctcata tggtgaataa ggacactgtc tattcagtgc
     2221 cgctagtaac ccagtgatct catctctacc agactgtagt gcaaatttta tgctgtgtaa
     2281 cttcttcaac agatctttcc acgaaactga ttccacccaa gaatcgtcca tcaagtcagc
     2341 aggaccgccc gacttgtcaa atgattcatc gtacatatct cccatgttga ttagcgcaag
     2401 tgattcagta acctgtagtt caacaagtct aatcgtccaa attaaaatga tcttatcagc
     2461 agacacaagt attatattta tgccatctct aatgataaca ttaaagactt agctagtttt
     2521 ctctaaagaa actagcaaaa ccccaacgtc cgtttagacg cttgcgtagg agaagttaat
     2581 taaaacgacc ccatcgtttt tgcggtagcg attaaaacca ccacctttct ttcattgcta
     2641 ctttcgtaag cgatacgtct caggttttcc tttgtatgag aagacaagtg ctttcttact
     2701 aacctaaaga accgaaaccc aaagtaacat ttcaaagata agcacaccag tcactttccc
     2761 gtacggtact aagctggata gttcaaaaag aaatattaca ctgacaaaaa cctcgtcagg
     2821 gagagggata aaggctgtgt tggataagga cgttctaccg tattctccaa cttgtcaatt
     2881 agtacattct actgcgatca ctgacatacc ccagccaggg caacaccata ggtgcaatgt
     2941 tttatcctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag
     3001 tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa
     3061 gacgtggttg gaacgtcttc tttttccacg atgttcctcg tgggtggggg tccatctttg
     3121 ggaccactgt cggtagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat
     3181 ttgtagaagc catcttcctt ttctactgtc ctttcgatga agtgacagat agctgggcaa
     3241 tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctctgg
     3301 tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg
     3361 ttgaccatca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
     3421 gatgttcctc gtgggtgggg gtccatcttt gggaccactg tcggtagagg catcttgaac
     3481 gatagccttt cctttatcgc aatgatggca tttgtagaag ccatcttcct tttctaccgt
     3541 cctttcgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
     3601 cctttgttga aaagtctcaa tagccctctg gtcttctgag actgtatctt tgatattctt
     3661 ggagtagacg agagtgtcgt gctccaccat gttgacctgc aggcatgcaa gcttggcgcg
     3721 ccgggcccaa catggtggcc taggaattaa ttcggcgtta attcagtaca ttaaaaacgt
     3781 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc
     3841 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg
     3901 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct
     3961 catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg
     4021 atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcac
     4081 cgcggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt
     4141 gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc
     4201 gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat
     4261 aataaatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct
     4321 gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg
     4381 aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac
     4441 gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact
     4501 ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct
     4561 cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca
     4621 tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc
     4681 gcttagccga attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact
     4741 gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa
     4801 agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga
     4861 aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt
     4921 atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg
     4981 agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt
     5041 tactggatga attgttttag tacctagaat gcatgaccaa aatcccttaa cgctgagaga
     5101 tcccctcata atttccccaa aacgtaacca tgtgtgaata gattttgagt aagcagggtt
     5161 gcagccacga gtgagtcttc ccttgttatt gtgtagccag aatgccgcaa aacttccatg
     5221 cctaagcgaa ctgttgagag tacgtttcga tttctgactg tgttagcctg gaagtgcttg
     5281 tcccaacctt gtttctgagc atgaacgccc gcaagccaac atgttagttg aagcatcagg
     5341 gcgattagca gcatgatatc aaaacgctct gagctgctcg ttcggctatg gcgtaggcct
     5401 agtccgtagg caggactttt caagtctcgg aaggtttctt caatctgcat tcgcttcgaa
     5461 tagatattaa caagttgttt gggtgttcga atttcaacag gtaagttagt tgctagaatc
     5521 catggctcct ttgccgacgc tgagtagatt ttaggtgacg ggtggtgaca atgagtccgt
     5581 gtcgagcgct gattttttcg gcctttagag cgagatttat acaatagaat ttggcatgag
     5641 attggattgc ttttagtcag cctcttatag cctaaagtct ttgagtgact agatgacata
     5701 tcatgtaagt tgctgatagg tttccagttt tccgctccta ggtctgcata ttgtactttt
     5761 cctcttactc gacttaacca gtaccaaccc agcttctcaa cggatttata ccatggcact
     5821 ttaaagccag catcactgac aatgagcggt gtggtgttac tcggtagaat gctcgcaagg
     5881 tcggctagaa attggtcatg agctttcttt gaacattgct ctgaaagcgg gaacgctttc
     5941 tcataaagag taacagaacg accgtgtagt gcgactgaag ctcgcaatac cataagccgt
     6001 ttttgctcac ggatatcaga ccagtcaaca agtacaatgg gcatcgtatt gcccgaacag
     6061 ataaagctag catgccaacg gtatacagcg agtcgctctt tgtggaggtg acgattacct
     6121 aacaatcggt cgattcgttt gatgttatgt tttgttctcg ctttggttgg caggttacgg
     6181 ccaagttcgg taagagtgag agttttacag tcaagtaagg cgtggcaagc caacgttaag
     6241 ctgttgagtc gttttaagtg taattcgggg cagaattggt aaagagagtc gtgtaaaata
     6301 tcgagttcgc acattttgtt gtctgattat tgatttttgg cgaaaccatt tgatcatatg
     6361 acaagatgtg tatctacctt aacttaatga ttttgataaa aatcattagg ggattcatca
     6421 gcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6481 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6541 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6601 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     6661 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6721 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6781 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6841 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6901 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6961 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7021 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7081 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7141 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7201 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7261 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     7321 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     7381 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     7441 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     7501 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
     7561 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     7621 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     7681 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     7741 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     7801 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     7861 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     7921 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt
     7981 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     8041 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     8101 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     8161 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     8221 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     8281 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     8341 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     8401 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     8461 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     8521 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     8581 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     8641 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     8701 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     8761 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
     8821 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
     8881 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
     8941 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
     9001 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
     9061 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
     9121 gcaggcaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
     9181 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
     9241 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
     9301 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
     9361 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
     9421 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
     9481 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
     9541 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
     9601 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
     9661 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
     9721 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
     9781 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
     9841 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
     9901 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
     9961 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
    10021 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
    10081 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
    10141 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
    10201 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
    10261 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
    10321 cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc
    10381 tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt
    10441 gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct
    10501 cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg
    10561 cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc
    10621 cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg
    10681 ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga
    10741 ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg
    10801 gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga
    10861 tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca
    10921 ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc
    10981 gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg
    11041 cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag
    11101 cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta
    11161 tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg
    11221 taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc
    11281 cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga ggggaaccct
    11341 gtggttggca tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata
    11401 tccgattatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa
    11461 acactgatag tttaattccc gatctagtaa catagatgac accgcgcgcg ataatttatc
    11521 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
    11581 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
    11641 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
    11701 taagaaactt tattgccaaa
//