pHDM-VSV-G

LOCUS       pHDM-VSV-G        6240 bp DNA     circular SYN 25-AUG-2024
DEFINITION  Encodes VSV-G protein for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman 
            J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter 
            G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt 
            AG, Iafrate AJ, Balazs AB
  TITLE     COVID-19-neutralizing antibodies predict disease severity and 
            survival.
  JOURNAL   Cell. 2021 Jan 21;184(2):476-488.e11. doi: 
            10.1016/j.cell.2020.12.015. Epub 2020 Dec 15.
  PUBMED    33412089
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2024 from SnapGene Server 7.0.3
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.015"; journalName: "Cell"; date: 
            "2021-01-21- 21"; volume: "184"; issue: "2"; pages: "476-488.e11"
FEATURES             Location/Qualifiers
     source          1..6240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          318..793
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             855..2390
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     primer_bind     complement(2549..2565)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2550..2566)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    2659..3053
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(3387..3405)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3473..3577
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3578..4438
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3796..3815)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4609..5197
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5098..5117
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5861..6240
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctg
      301 atcctgagaa cttcagggtg agtctatggg acccttgatg ttttctttcc ccttcttttc
      361 tatggttaag ttcatgtcat aggaagggga gaagtaacag ggtacacata ttgaccaaat
      421 cagggtaatt ttgcatttgt aattttaaaa aatgctttct tcttttaata tacttttttg
      481 tttatcttat ttctaatact ttccctaatc tctttctttc agggcaataa tgatacaatg
      541 tatcatgcct ctttgcacca ttctaaagaa taacagtgat aatttctggg ttaaggcaat
      601 agcaatattt ctgcatataa atatttctgc atataaattg taactgatgt aagaggtttc
      661 atattgctaa tagcagctac aatccagcta ccattctgct tttattttat ggttgggata
      721 aggctggatt attctgagtc caagctaggc ccttttgcta atcatgttca tacctcttat
      781 cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa
      841 agaattctga cactatgaag tgccttttgt acttagcctt tttattcatt ggggtgaatt
      901 gcaagttcac catagttttt ccacacaacc aaaaaggaaa ctggaaaaat gttccttcta
      961 attaccatta ttgcccgtca agctcagatt taaattggca taatgactta ataggcacag
     1021 ccttacaagt caaaatgccc aagagtcaca aggctattca agcagacggt tggatgtgtc
     1081 atgcttccaa atgggtcact acttgtgatt tccgctggta tggaccgaag tatataacac
     1141 attccatccg atccttcact ccatctgtag aacaatgcaa ggaaagcatt gaacaaacga
     1201 aacaaggaac ttggctgaat ccaggcttcc ctcctcaaag ttgtggatat gcaactgtga
     1261 cggatgccga agcagtgatt gtccaggtga ctcctcacca tgtgctggtt gatgaataca
     1321 caggagaatg ggttgattca cagttcatca acggaaaatg cagcaattac atatgcccca
     1381 ctgtccataa ctctacaacc tggcattctg actataaggt caaagggcta tgtgattcta
     1441 acctcatttc catggacatc accttcttct cagaggacgg agagctatca tccctgggaa
     1501 aggagggcac agggttcaga agtaactact ttgcttatga aactggaggc aaggcctgca
     1561 aaatgcaata ctgcaagcat tggggagtca gactcccatc aggtgtctgg ttcgagatgg
     1621 ctgataagga tctctttgct gcagccagat tccctgaatg cccagaaggg tcaagtatct
     1681 ctgctccatc tcagacctca gtggatgtaa gtctaattca ggacgttgag aggatcttgg
     1741 attattccct ctgccaagaa acctggagca aaatcagagc gggtcttcca atctctccag
     1801 tggatctcag ctatcttgct cctaaaaacc caggaaccgg tcctgctttc accataatca
     1861 atggtaccct aaaatacttt gagaccagat acatcagagt cgatattgct gctccaatcc
     1921 tctcaagaat ggtcggaatg atcagtggaa ctaccacaga aagggaactg tgggatgact
     1981 gggcaccata tgaagacgtg gaaattggac ccaatggagt tctgaggacc agttcaggat
     2041 ataagtttcc tttatacatg attggacatg gtatgttgga ctccgatctt catcttagct
     2101 caaaggctca ggtgttcgaa catcctcaca ttcaagacgc tgcttcgcaa cttcctgatg
     2161 atgagagttt attttttggt gatactgggc tatccaaaaa tccaatcgag cttgtagaag
     2221 gttggttcag tagttggaaa agctctattg cctctttttt ctttatcata gggttaatca
     2281 ttggactatt cttggttctc cgagttggta tccatctttg cattaaatta aagcacacca
     2341 agaaaagaca gatttataca gacatagaga tgaaccgact tggaaagtaa ctcaaatcct
     2401 gcacaacaga ttcttcatgt ttggaccaaa tcaacttgtg ataccatgct caaagaggcc
     2461 tcaattatat ttgagttttt aatttttatg naaaaaaaaa aaaaaaacgg aattccgcgg
     2521 gcggccgctc tagaggatcc aagcttatcg ataccgtcga cctcgagggc ccagatctaa
     2581 ttcaccccac cagtgcaggc tgcctatcag aaagtggtgg ctggtgtggc taatgccctg
     2641 gcccacaagt atcactaagc tcgctttctt gctgtccaat ttctattaaa ggttcctttg
     2701 ttccctaagt ccaactacta aactggggga tattatgaag ggccttgagc atctggattc
     2761 tgcctaataa aaaacattta ttttcattgc aatgatgtat ttaaattatt tctgaatatt
     2821 ttactaaaaa gggaatgtgg gaggtcagtg catttaaaac ataaagaaat gaagagctag
     2881 ttcaaacctt gggaaaatac actatatctt aaactccatg aaagaaggtg aggctgcaaa
     2941 cagctaatgc acattggcaa cagcccctga tgcctatgcc ttattcatcc ctcagaaaag
     3001 gattcaagta gaggcttgat ttggaggtta aagttttgct atgctgtatt ttacattact
     3061 tattgtttta gctgtcctca tgaatgtctt ttcactaccc atttgcttat cctgcatctc
     3121 tcagccttga ctccactcag ttctcttgct tagagatacc acctttcccc tgaagtgttc
     3181 cttccatgtt ttacggcgag atggtttctc ctcgcctggc cactcagcct tagttgtctc
     3241 tgttgtctta tagaggtcta cttgaagaag gaaaaacagg gggcatggtt tgactgtcct
     3301 gtgagccctt cttccctgcc tcccccactc acagtgaccc ggaatccctc gacatggcag
     3361 tctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     3421 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     3481 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3541 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3601 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     3661 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     3721 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     3781 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     3841 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     3901 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     3961 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4021 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4081 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4141 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4201 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4261 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4321 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4381 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     4441 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4501 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4561 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4621 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4681 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4741 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4801 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4861 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4921 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4981 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5041 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5101 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5161 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg gagatgcgcc
     5221 gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg
     5281 cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag
     5341 cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa
     5401 cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt
     5461 gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg aagttaggct
     5521 ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga
     5581 cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg
     5641 gaaggccatc cagcctcgcg tcggggagct ttttgcaaaa gcctaggcct ccaaaaaagc
     5701 ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
     5761 aaaaaattag tcagccatga gcttggccca ttgcatacgt tgtatccata tcataatatg
     5821 tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt
     5881 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     5941 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     6001 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     6061 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     6121 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     6181 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
//