| 质粒名称: | pcDNA5-Flag-BRD4-WT |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 90331 |
| 存储实验室: | Kornelia Polyak |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA5 frt/to |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5137 bp |
| 完整质粒大小: | 9226 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | BRD4 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 4089 bp |
| 基因突变: | Wild type |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | Flag （插入件上的N端子） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA5-Flag-BRD4-WT was a gift from Kornelia Polyak (Addgene plasmid # 90331 ; http://n2t.net/addgene:90331 ; RRID:Addgene_90331) |
| 参考文献部分: | Response and resistance to BET bromodomain inhibitors in triple-negative breast cancer. Shu S, Lin CY, He HH, Witwicki RM, Tabassum DP, Roberts JM, Janiszewska M, Huh SJ, Liang Y, Ryan J, Doherty E, Mohammed H, Guo H, Stover DG, Ekram MB, Peluffo G, Brown J, D'Santos C, Krop IE, Dillon D, McKeown M, Ott C, Qi J, Ni M, Rao PK, Duarte M, Wu SY, Chiang CM, Anders L, Young RA, Winer EP, Letai A, Barry WT, Carroll JS, Long HW, Brown M, Liu XS, Meyer CA, Bradner JE, Polyak K. Nature. 2016 Jan 21;529(7586):413-7. doi: 10.1038/nature16508. Epub 2016 Jan 6. 10.1038/nature16508 PubMed 26735014 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcDNA5-Flag-BRD4-WT 9333 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION expresses Wild type BRD4.
ACCESSION .
VERSION .
KEYWORDS pcDNA5-Flag-BRD4-WT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9333)
AUTHORS Shu S, Lin CY, He HH, Witwicki RM, Tabassum DP, Roberts JM,
Janiszewska M, Huh SJ, Liang Y, Ryan J, Doherty E, Mohammed H, Guo
H, Stover DG, Ekram MB, Peluffo G, Brown J, D'Santos C, Krop IE,
Dillon D, McKeown M, Ott C, Qi J, Ni M, Rao PK, Duarte M, Wu SY,
Chiang CM, Anders L, Young RA, Winer EP, Letai A, Barry WT, Carroll
JS, Long HW, Brown M, Liu XS, Meyer CA, Bradner JE, Polyak K
TITLE Response and resistance to BET bromodomain inhibitors in
triple-negative breast cancer.
JOURNAL Nature. 2016 Jan 21;529(7586):413-7. doi: 10.1038/nature16508. Epub
2016 Jan 6.
PUBMED 26735014
REFERENCE 2 (bases 1 to 9333)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9333
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(23..42)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 214..593
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 594..797
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 748..768
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 799..817
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 820..838
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 848..872
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 971..994
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 3212..3229
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(5264..5281)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5270..5494
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(5627..5646)
/label=F1ori-R
/note="F1 origin, reverse primer"
protein_bind 5778..5825
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
CDS 5833..6855
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
"
polyA_signal 6985..7106
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7022..7041)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7076..7095
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7155..7171)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7155..7171)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7168..7190)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7179..7195
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7203..7233)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7248..7269
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7386..7403)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7557..8145)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7637..7656)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8316..9176)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8939..8958
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9177..9281)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
61 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
121 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
181 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
241 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
301 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
361 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
421 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
481 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
541 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
601 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
661 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
721 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
781 tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc
841 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
901 acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacttaagc
961 ttccaccatg gattacaagg atgacgacga taagggatct atgtctgcgg agagcggccc
1021 tgggacgaga ttgagaaatc tgccagtaat gggggatgga ctagaaactt cccaaatgtc
1081 tacaacacag gcccaggccc aaccccagcc agccaacgca gccagcacca accccccgcc
1141 cccagagacc tccaacccta acaagcccaa gaggcagacc aaccaactgc aatacctgct
1201 cagagtggtg ctcaagacac tatggaaaca ccagtttgca tggcctttcc agcagcctgt
1261 ggatgccgtc aagctgaacc tccctgatta ctataagatc attaaaacgc ctatggatat
1321 gggaacaata aagaagcgct tggaaaacaa ctattactgg aatgctcagg aatgtatcca
1381 ggacttcaac actatgttta caaattgtta catctacaac aagcctggag atgacatagt
1441 cttaatggca gaagctctgg aaaagctctt cttgcaaaaa ataaatgagc tacccacaga
1501 agaaaccgag atcatgatag tccaggcaaa aggaagagga cgtgggagga aagaaacagg
1561 gacagcaaaa cctggcgttt ccacggtacc aaacacaact caagcatcga ctcctccgca
1621 gacccagacc cctcagccga atcctcctcc tgtgcaggcc acgcctcacc ccttccctgc
1681 cgtcaccccg gacctcatcg tccagacccc tgtcatgaca gtggtgcctc cccagccact
1741 gcagacgccc ccgccagtgc ccccccagcc acaaccccca cccgctccag ctccccagcc
1801 cgtacagagc cacccaccca tcatcgcggc caccccacag cctgtgaaga caaagaaggg
1861 agtgaagagg aaagcagaca ccaccacccc caccaccatt gaccccattc acgagccacc
1921 ctcgctgccc ccggagccca agaccaccaa gctgggccag cggcgggaga gcagccggcc
1981 tgtgaaacct ccaaagaagg acgtgcccga ctctcagcag cacccagcac cagagaagag
2041 cagcaaggtc tcggagcagc tcaagtgctg cagcggcatc ctcaaggaga tgtttgccaa
2101 gaagcacgcc gcctacgcct ggcccttcta caagcctgtg gacgtggagg cactgggcct
2161 acacgactac tgtgacatca tcaagcaccc catggacatg agcacaatca agtctaaact
2221 ggaggcccgt gagtaccgtg atgctcagga gtttggtgct gacgtccgat tgatgttctc
2281 caactgctat aagtacaacc ctcctgacca tgaggtggtg gccatggccc gcaagctcca
2341 ggatgtgttc gaaatgcgct ttgccaagat gccggacgag cctgaggagc cagtggtggc
2401 cgtgtcctcc ccggcagtgc cccctcccac caaggttgtg gccccgccct catccagcga
2461 cagcagcagc gatagctcct cggacagtga cagttcgact gatgactctg aggaggagcg
2521 agcccagcgg ctggctgagc tccaggagca gctcaaagcc gtgcacgagc agcttgcagc
2581 cctctctcag ccccagcaga acaaaccaaa gaaaaaggag aaagacaaga aggaaaagaa
2641 aaaagaaaag cacaaaagga aagaggaagt ggaagagaat aaaaaaagca aagccaagga
2701 acctcctcct aaaaagacga agaaaaataa tagcagcaac agcaatgtga gcaagaagga
2761 gccagcgccc atgaagagca agccccctcc cacgtatgag tcggaggaag aggacaagtg
2821 caagcctatg tcctatgagg agaagcggca gctcagcttg gacatcaaca agctccccgg
2881 cgagaagctg ggccgcgtgg tgcacatcat ccagtcacgg gagccctccc tgaagaattc
2941 caaccccgac gagattgaaa tcgactttga gaccctgaag ccgtccacac tgcgtgagct
3001 ggagcgctat gtcacctcct gtttgcggaa gaaaaggaaa cctcaagctg agaaagttga
3061 tgtgattgcc ggctcctcca agatgaaggg cttctcgtcc tcagagtcgg agagctccag
3121 tgagtccagc tcctctgaca gcgaagactc cgaaacagag atggctccga agtcaaaaaa
3181 gaaggggcac cccgggaggg agcagaagaa gcaccatcat caccaccatc agcagatgca
3241 gcaggccccg gctcctgtgc cccagcagcc gcccccgcct ccccagcagc ccccaccgcc
3301 tccacctccg cagcagcaac agcagccgcc acccccgcct cccccaccct ccatgccgca
3361 gcaggcagcc ccggcgatga agtcctcgcc cccacccttc attgccaccc aggtgcccgt
3421 cctggagccc cagctcccag gcagcgtctt tgaccccatc ggccacttca cccagcccat
3481 cctgcacctg ccgcagcctg agctgccccc tcacctgccc cagccgcctg agcacagcac
3541 tccaccccat ctcaaccagc acgcagtggt ctctcctcca gctttgcaca acgcactacc
3601 ccagcagcca tcacggccca gcaaccgagc cgctgccctg cctcccaagc ccgcccggcc
3661 cccagccgtg tcaccagcct tgacccaaac acccctgctc ccacagcccc ccatggccca
3721 acccccccaa gtgctgctgg aggatgaaga gccacctgcc ccacccctca cctccatgca
3781 gatgcagctg tacctgcagc agctgcagaa ggtgcagccc cctacgccgc tactcccttc
3841 cgtgaaggtg cagtcccagc ccccaccccc cctgccgccc ccaccccacc cctctgtgca
3901 gcagcagctg cagcagcagc cgccaccacc cccaccaccc cagccccagc ctccacccca
3961 gcagcagcat cagccccctc cacggcccgt gcacttgcag cccatgcagt tttccaccca
4021 catccaacag cccccgccac cccagggcca gcagcccccc catccgcccc caggccagca
4081 gccacccccg ccgcagcctg ccaagcctca gcaagtcatc cagcaccacc attcaccccg
4141 gcaccacaag tcggacccct actcaaccgg tcacctccgc gaagccccct ccccgcttat
4201 gatacattcc ccccagatgt cacagttcca gagcctgacc caccagtctc caccccagca
4261 aaacgtccag cctaagaaac aggagctgcg tgctgcctcc gtggtccagc cccagcccct
4321 cgtggtggtg aaggaggaga agatccactc acccatcatc cgcagcgagc ccttcagccc
4381 ctcgctgcgg ccggagcccc ccaagcaccc ggagagcatc aaggcccccg tccacctgcc
4441 ccagcggccg gaaatgaagc ctgtggatgt cgggaggcct gtgatccggc ccccagagca
4501 gaacgcaccg ccaccagggg cccctgacaa ggacaaacag aaacaggagc cgaagactcc
4561 agttgcgccc aaaaaggacc tgaaaatcaa gaacatgggc tcctgggcca gcctagtgca
4621 gaagcatccg accaccccct cctccacagc caagtcatcc agcgacagct tcgagcagtt
4681 ccgccgcgcc gctcgggaga aagaggagcg tgagaaggcc ctgaaggctc aggccgagca
4741 cgctgagaag gagaaggagc ggctgcggca ggagcgcatg aggagccgag aggacgagga
4801 tgcgctggag caggcccggc gggcccatga ggaggcacgt cggcgccagg agcagcagca
4861 gcagcagcgc caggagcaac agcagcagca gcaacagcaa gcagctgcgg tggctgccgc
4921 cgccacccca caggcccaga gctcccagcc ccagtccatg ctggaccagc agagggagtt
4981 ggcccggaag cgggagcagg agcgaagacg ccgggaagcc atggcagcta ccattgacat
5041 gaatttccag agtgatctat tgtcaatatt tgaagaaaat cttttctgag cgcacctagg
5101 tggcttctga ctttgatttt ctggcaaaac attgactttc catagtgtta ggggcggtgg
5161 tggaggtggg atcagcggcc aggggatgcc tcagggcctg gccctcctgc atgcaagctt
5221 cagctgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
5281 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
5341 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
5401 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
5461 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
5521 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
5581 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
5641 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
5701 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
5761 gatggttcac gtacctagaa gttcctattc cgaagttcct attctctaga aagtatagga
5821 acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
5881 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
5941 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
6001 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
6061 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
6121 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
6181 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
6241 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
6301 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
6361 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
6421 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
6481 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
6541 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
6601 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
6661 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
6721 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
6781 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
6841 agggcaaagg aatagcacgt actacgagat ttcgattcca ccgccgcctt ctatgaaagg
6901 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
6961 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
7021 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
7081 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
7141 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
7201 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
7261 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
7321 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
7381 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
7441 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
7501 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
7561 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
7621 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
7681 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
7741 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
7801 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
7861 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
7921 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
7981 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
8041 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
8101 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
8161 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
8221 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
8281 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
8341 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
8401 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
8461 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
8521 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
8581 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
8641 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
8701 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
8761 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
8821 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
8881 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
8941 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
9001 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
9061 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
9121 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
9181 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
9241 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
9301 ccacctgacg tcgacggatc gggagatctc ccg
//
