pT7-Guide-IVT

价格:1500元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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pT7-Guide-IVT载体质粒基本信息

出品公司: Origene
载体名称: pT7-Guide-IVT
质粒类型: 体外转录Crispr/Cas9载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: T7
载体大小: 3.4 kb
5’测序引物: T7
3’测序引物: M13-F
载体标签: --
载体抗性: 氨苄青霉素
克隆菌株: DH5 alpha; 等
产品目录号: GE100025
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒
备注:
pT7-Guide-IVT载体是T7启动子驱动的gRNA体外表达克隆载体
 
pT7-Guide-IVT载体在克隆靶序列后,可以通过体外转录产生gRNA
 
BsmB I位点用于pT7-Guide-IVT质粒靶序列克隆位点
 
对于通过显微注射或mRNA转染进行的mRNA介导的基因组编辑,需要体外表达Cas9 mRNA和gRNA转录本。T7驱动的体外表达Cas9 mRNA可以从单独的载体pT7-Cas9中产生


pT7-Guide-IVT质粒是体外表达gRNA,M13 Forward测序引物可用于克隆后对gRNA靶序列进行测序。

相关载体:

pT7-Cas9

pT7-Guide-IVT质粒图谱载体图谱和pT7-Guide-IVT载体序列质粒序列多克隆位点信息

pT7-Guide-IVT载体图谱

pT7-Guide-IVT载体图谱

pT7-Guide-IVT质粒载体简介

pT7-Guide-IVT质粒序列载体序列

LOCUS       pT7-Guide-IVT                3444 bp DNA     circular SYN 07-MAR-2024
DEFINITION  In vitro transcription vector with the T7 promoter, for producing a 
            guide RNA (gRNA).
ACCESSION   .
VERSION     .
KEYWORDS    pT7-Guide-IVT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   OriGene
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2024 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..3444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(35..51)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      264..719
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        1001..1105
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             1106..1966
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 1106 .. 1174 / #ccffcc / signal sequence
                        2: 1175 .. 1966 / #ccffcc
                     Cleavage site after base 1174"                     
     rep_origin      2137..2725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     promoter        3228..3246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     misc_RNA        3300..3375
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
                     /note="color: #00ccff; direction: RIGHT"
ORIGIN
        1 gaaagtcctc tccactgact gtagcctcca attcactggc cgtcgtttta caacgtcgtg
       61 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
      121 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
      181 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
      241 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      301 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      361 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      421 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
      481 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
      541 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
      601 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
      661 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
      721 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
      781 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
      841 agctgtgacc gtcaacggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
      901 cgcgacccga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      961 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     1021 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1081 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     1141 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     1201 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1261 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1321 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1381 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1441 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1501 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1561 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1621 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1681 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1741 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1801 atctggagcc ggtgagcgtg gttctcgcgg tatcattgca gcactggggc cagatggtaa
     1861 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1921 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1981 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2041 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2101 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2161 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2221 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2281 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2341 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2401 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2461 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2521 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2581 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2641 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2701 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2761 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2821 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2881 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     2941 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     3001 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     3061 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     3121 ctatgaccat gattacgcca agctccttcc tcttccagcc cttcctcttt cgggcggcag
     3181 caagatccct tctgatctaa agtccaaccg gcgagagtga ggaattctaa tacgactcac
     3241 tatagggaga cgtccactgg accaggcagc agcgtcagaa gacttttttg gaacgtctcg
     3301 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
     3361 gcaccgagtc ggtgctttta aaaagcttga attcgtgtcg catgaataac gggcagttat
     3421 caaatgagca cggcgacagc ctgt
//

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