| 出品公司: | Clontech |
|---|---|
| 载体名称: | pCRE-Luc |
| 质粒类型: | 信号通路报告系统载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5.7kb |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | 112159 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.
载体序列
LOCUS pCre-Luc 5688 bp DNA circular SYN 26-JAN-1999
DEFINITION Reporter vector pCRE-Luc, complete sequence.
ACCESSION AF053461
VERSION AF053461.1
KEYWORDS .
SOURCE Reporter vector pCRE-Luc
ORGANISM Reporter vector pCRE-Luc
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5688)
AUTHORS Zheng,C.-F.
TITLE pCRE-Luc plasmid
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5688)
AUTHORS Zheng,C.-F.
TITLE Direct Submission
JOURNAL Submitted (12-MAR-1998) Technical Services, Stratagene, 11011 N.
Torrey Pines, San Diego, CA 92037, USA
REFERENCE 3 (bases 1 to 5688)
AUTHORS Zheng,C.-F.
TITLE Direct Submission
JOURNAL Submitted (29-OCT-1998) Technical Services, Stratagene, 11011 N.
Torrey Pines, San Diego, CA 92037, USA
REMARK Sequence update by submitter
REFERENCE 4 (bases 1 to 5688)
AUTHORS Zheng,C.-F.
TITLE Direct Submission
JOURNAL Submitted (26-JAN-1999) Technical Services, Stratagene, 11011 N.
Torrey Pines, San Diego, CA 92037, USA
REMARK Sequence update by submitter
COMMENT On Jan 26, 1999 this sequence version replaced gi:3808229.
FEATURES Location/Qualifiers
source 1..5688
/organism="Reporter vector pCRE-Luc"
/mol_type="genomic DNA"
/host="Escherichia coli K12"
/db_xref="taxon:74544"
/note="designed for detection of activation of the cyclic
AMP response element (CRE) pathway in eukaryotic cells"
ORIGIN
1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
1561 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
1741 gcggagctat ggaaaaacgc cagcaacgcg cctttttacg gttcctggcc ttttgctggc
1801 cttttgctca catgttcttt cctgcgttat ccctgattct gtggataacc gtattaccgc
1861 ctttgagtgc tgataccgct cgccgcagcc gaacgaccga gcgcaagtca gtgagcgagg
1921 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
1981 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
2041 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
2101 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
2161 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggatca
2221 gccatatcac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
2281 acctgaaaca taaaatgaat gcaattgttg ttaacttgtt tattgcagct tataatggtt
2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttcact gcattctagt
2401 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcagcca tatcacattt
2461 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
2521 atgaatgcaa ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
2581 agcatcacaa atttcacaaa taaagcaatt tttcactgca ttctattgtg gtttgtccaa
2641 actcatcaat gtatcttatc atgtctggat ccaagctagc cagcctgacg tcagaggcct
2701 gacgtcagag agcctgacgt cagagagcct gacgtcagag cggagactct agagggtata
2761 taatggaagc tcgaattcca gcttggcatt ccggtactgt tggtaaaatg gaagacgcca
2821 aaaacataaa gaaaggcccg gcgccattct atcctctaga ggatggaacc gctggagagc
2881 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
2941 cacatatcga ggtgaacatc acgtacgcgg aatacttcga aatgtccgtt cggttggcag
3001 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
3061 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
3121 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gaacatttcg cagcctaccg
3181 tagtgtttgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa
3241 taatccagaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
3301 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtaccagagt
3361 cctttgatcg tgacaaaaca attgcactga taatgaattc ctctggatct actgggttac
3421 ctaagggtgt ggcccttccg catagaactg cctgcgtcag attctcgcat gccagagatc
3481 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc
3541 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
3601 tgtatagatt tgaagaagag ctgtttttac gatcccttca ggattacaaa attcaaagtg
3661 cgttgctagt accaacccta ttttcattct tcgccaaaag cactctgatt gacaaatacg
3721 atttatctaa tttacacgaa attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg
3781 aagcggttgc aaaacgcttc catcttccag ggatacgaca aggatatggg ctcactgaga
3841 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag
3901 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
3961 atcagagagg cgaattatgt gtcagaggac ctatgattat gtccggttat gtaaacaatc
4021 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacatagctt
4081 actgggacga agacgaacac ttcttcatag ttgaccgctt gaagtcttta attaaataca
4141 aaggatatca ggtggccccc gctgaattgg aatcgatatt gttacaacac cccaacatct
4201 tcgacgcggg cgtggcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
4261 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
4321 aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
4381 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
4441 gcggaaagtc caaattgtaa aatgtaactg tattcagcga tgacgaaatt cttagctatt
4501 gtaatactct agaggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
4561 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
4621 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aacttatgaa
4681 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
4741 tctagtgatg atgaggctac tgctgactct caacattcta ctctcaaaag aagagaaagg
4801 tagagaccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
4861 aatagaactc ttgcttgctt tgctatttac aaccacaaag gaaaaagctg cactgctata
4921 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca
4981 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc
5041 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat
5101 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg
5161 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
5221 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
5281 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
5341 tatcttatca tgtctggatc cccaggaagc tcctctgtgt cctcataaac cctaacctcc
5401 tctacttgag aggacattcc aatcataggc tgcccatcca ccctctgtgt cctcctgtta
5461 attaggtcac ttaacaaaaa ggaaattggg taggggtttt tcacagaccg ctttctaagg
5521 ggtaatttta aaatatctgg gaagtccctt ccactgctgt gttccagaag tgttggtaaa
5581 cagcccacaa atgtcaacag cagaaacata caagctgtca ctttgcacaa agggcctcgt
5641 gatacgccta tttttatagg ttaatgtcat gataataatg gtttctta
//
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