| 质粒名称: | V5-APEX2-SENP2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 129276 |
| 存储实验室: | Alice Ting |
|
载体骨架基本信息 |
|
| 载体名称: | pLX304 |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | V5-APEX2-SENP2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | CMV promoter |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson 克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | CMV forward |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | V5-APEX2-SENP2 was a gift from Alice Ting (Addgene plasmid # 129276 ; http://n2t.net/addgene:129276 ; RRID:Addgene_129276) |
| 参考文献部分: | Atlas of Subcellular RNA Localization Revealed by APEX-Seq. Fazal FM, Han S, Parker KR, Kaewsapsak P, Xu J, Boettiger AN, Chang HY, Ting AY. Cell. 2019 Jun 14. pii: S0092-8674(19)30555-0. doi: 10.1016/j.cell.2019.05.027. 10.1016/j.cell.2019.05.027 PubMed 31230715 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS V5-APEX2-SENP2 10263 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expression of APEX2 at nuclear pore.
ACCESSION .
VERSION .
KEYWORDS V5-APEX2-SENP2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10263)
AUTHORS Fazal FM, Han S, Parker KR, Kaewsapsak P, Xu J, Boettiger AN, Chang
HY, Ting AY
TITLE Atlas of Subcellular RNA Localization Revealed by APEX-Seq.
JOURNAL Cell. 2019 Jun 14. pii: S0092-8674(19)30555-0. doi:
10.1016/j.cell.2019.05.027.
PUBMED 31230715
REFERENCE 2 (bases 1 to 10263)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10263
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 118..137
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 371..388
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 505..526
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 541..571
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 579..595
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 584..606
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 603..619
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 603..619
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 638..658
/label=T3
/note="T3 promoter, forward primer"
promoter 640..658
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 686..912
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 913..1093
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1140..1265
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1758..1991
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2176..2220
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
promoter 2395..2895
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 2814..2832
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 2907..3302
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 3362..3479
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 3596..3899
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3900..4103
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4054..4074
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 4126..4150
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 4171..4212
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 4213..4959
/codon_start=1
/product="soybean ascorbate peroxidase, engineered to serve
as a monomeric tag for electron microscopy and live-cell
proteomics (Lam et al., 2015)"
/label=APEX2
/note="more active than the original APEX due to the A134P
mutation"
/translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
YAADEDAFFADYAEAHQKLSELGFADA"
misc_feature 6801..7389
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6854..6874)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(7272..7283)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 7461..7694
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 7772..7893
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7809..7828)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7863..7882
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 7933..8068
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7995..8014
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(8088..8107)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(8089..8107)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(8117..8134)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(8117..8133)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8126..8148)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 8275..8730
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8362..8381)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8572..8593
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 8756..8860
/gene="bla"
/label=AmpR promoter
CDS 8861..9721
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(9079..9098)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9892..217
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
61 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
121 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
181 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
241 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
301 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
361 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
421 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
481 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
541 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
601 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
661 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
721 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
781 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
841 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
901 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
961 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
1021 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
1081 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
1141 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
1201 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
1261 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
1321 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
1381 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
1441 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
1501 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
1561 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
1621 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
1681 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
1741 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
1801 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
1861 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
1921 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
1981 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
2041 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
2101 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
2161 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
2221 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
2281 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
2341 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccttgcgc
2401 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
2461 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
2521 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
2581 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
2641 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
2701 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
2761 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
2821 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
2881 gacctctctc cccagggggt accaccatgg ccaagccttt gtctcaagaa gaatccaccc
2941 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
3001 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
3061 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
3121 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
3181 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
3241 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
3301 gcctgcagct gcagtagtaa gaattctaga tcttgagaca aatggcagta ttcatccaca
3361 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
3421 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
3481 gggtttatta cagggacagc agagatccac tttggcgccg gctcgagggg gcccggggtt
3541 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
3601 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
3661 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
3721 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
3781 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
3841 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
3901 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
3961 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
4021 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
4081 gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag tttgtacaaa
4141 aaagcaggct tcgaaggaga tagaaccatg ggcaagccca tccccaaccc cctgctgggc
4201 ctggacagca ccggaaagtc ttacccaact gtgagtgctg attaccagga cgccgttgag
4261 aaggcgaaga agaagctcag aggcttcatc gctgagaaga gatgcgctcc tctaatgctc
4321 cgtttggcat tccactctgc tggaaccttt gacaagggca cgaagaccgg tggacccttc
4381 ggaaccatca agcaccctgc cgaactggct cacagcgcta acaacggtct tgacatcgct
4441 gttaggcttt tggagccact caaggcggag ttccctattt tgagctacgc cgatttctac
4501 cagttggctg gcgttgttgc cgttgaggtc acgggtggac ctaaggttcc attccaccct
4561 ggaagagagg acaagcctga gccaccacca gagggtcgct tgcctgatcc cactaagggt
4621 tctgaccatt tgagagatgt gtttggcaaa gctatggggc ttactgacca agatatcgtt
4681 gctctatctg ggggtcacac tattggagct gcacacaagg agcgttctgg atttgagggt
4741 ccctggacct ctaatcctct tattttcgac aactcatact tcacggagtt gttgagtggt
4801 gagaaggaag gtctccttca gctaccttct gacaaggctc ttttgtctga ccctgtattc
4861 cgccctctcg ttgataaata tgcagcggac gaagatgcct tctttgctga ttacgctgag
4921 gctcaccaaa agctttccga gcttgggttt gctgatgcct ccggccggac tcagatctcg
4981 agctcaagct tcgaattcat gtacagatgg ctggttagga ttctcggcac cattttccgt
5041 ttctgcgacc ggtcggtgcc ccctgcccgg gccctcctga agaggcggcg ctcagacagc
5101 actctgtttt ctacagtgga cactgatgaa ataccagcca aaagaccaag attagattgc
5161 tttattcacc aagtgaaaaa cagtctctac aatgctgcca gcttatttgg attcccattc
5221 cagctgacca caaagcccat ggtaacttct gcttgtaatg gaacacggaa tgtggcccct
5281 tcaggagagg tattttcgaa ctcttcatct tgtgaactga caggttctgg atcctggaac
5341 aacatgctga aactgggtaa taaatctcct aatggaataa gtgactatcc aaagatcaga
5401 gtgacagtta cccgagatca gccacgcaga gtcctgcctt cctttggttt tactttgaac
5461 tcagaaggct gtaatagaag accaggtggc cgtcgccata gcaaaggtaa tccagagagt
5521 tctttaatgt ggaaacctca ggaacaggct gtaacagaga tgatttctga agagagtggc
5581 aagggtctga ggcgtcccca ttgtactgtg gaggagggtg ttcaaaaaga ggaaagagag
5641 aagtaccgaa agttattgga acgacttaaa gaaagtggtc atggaaactc tgtctgtcct
5701 gtaacttcaa attatcacag ttctcaaaga agtcagatgg acacattaaa gaccaaaggc
5761 tggggggaag agcaaaatca cggagtcaaa acaactcagt ttgttccaaa acaatataga
5821 cttgttgaaa caaggggacc tctatgttca ttgagaagtg aaaagaggtg ttcaaagggg
5881 aaaattactg atacagagac gatggtcgga atcagatttg aaaatgaaag taggagggga
5941 taccaactgg agcctgacct atcagaagaa gtgtcggccc gactccgcct gggcagtgga
6001 agcaatggct tactcaggag gaaagtgtca ataattgaga caaaggaaaa gaattgctca
6061 ggcaaagaga gggacagaag aacggacgat ctccttgaac ttacagagga catggaaaag
6121 gaaatcagta atgccctagg ccatggccca caggatgaaa tcctaagtag tgctttcaaa
6181 ttgcgaatta ctcgaggaga tattcagaca ttaaagaact atcactggct caatgatgaa
6241 gtcattaatt tttacatgaa tcttctggtg gaaagaaata aaaagcaagg ctatccagca
6301 cttcatgtat tcagtacttt cttctatcct aaattaaagt ctgggggtta ccaagcagtg
6361 aaacgatgga ccaaaggggt aaatctcttt gaacaagaaa ttattctggt gcctattcat
6421 cggaaggtac attggagcct ggtggtgatt gacctaagaa aaaagtgtct taaatatctg
6481 gattctatgg gacaaaaggg ccacaggatc tgtgagattc tccttcagta tttacaggat
6541 gaaagtaaga ccaaaagaaa tagtgatctg aatcttttag agtggaccca tcacagcatg
6601 aaaccacacg agattcctca acagctgaat gggagtgatt gtggaatgtt tacttgtaaa
6661 tatgcagatt atatttctag ggacaaacct atcacattta ctcagcacca gatgcctctc
6721 ttccggaaga agatggtgtg ggaaatcctt catcagcagt tgctgtgagc tagcgctaac
6781 cggtggcgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
6841 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
6901 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
6961 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
7021 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
7081 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
7141 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
7201 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
7261 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
7321 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
7381 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
7441 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
7501 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
7561 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
7621 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
7681 gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt atttataact
7741 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
7801 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
7861 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
7921 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
7981 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
8041 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
8101 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
8161 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
8221 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
8281 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
8341 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
8401 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
8461 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
8521 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
8581 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
8641 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
8701 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
8761 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
8821 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
8881 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
8941 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
9001 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
9061 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
9121 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
9181 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
9241 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
9301 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
9361 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
9421 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
9481 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
9541 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
9601 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
9661 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
9721 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
9781 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
9841 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
9901 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
9961 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
10021 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
10081 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
10141 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
10201 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
10261 act
//
