| 出品公司: | -- |
|---|---|
| 载体名称: | pKANNIBAL |
| 质粒类型: | 植物表达载体；双表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CaMV 35S |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6063 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Exported 6063 bp ds-DNA circular SYN 25-AUG-2017
DEFINITION Vector for silencing genes in plants using intron-containing hairpin
RNA (hpRNA).
ACCESSION AJ311873
VERSION .
KEYWORDS pKANNIBAL
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6063)
AUTHORS Wesley SV, Helliwell CA, Smith NA, Wang MB, Rouse DT, Liu Q, Gooding
PS, Singh SP, Abbott D, Stoutjesdijk PA, Robinson SP, Gleave AP,
Green AG, Waterhouse PM.
TITLE Construct design for efficient, effective and high-throughput gene
silencing in plants.
JOURNAL Plant J. 2001;27:581-90.
PUBMED 11576441
REFERENCE 2 (bases 1 to 6063)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Friday, August 25, 2017 from SnapGene Viewer 3.2.1
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6063
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
rep_origin complement(215..643)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 670..774
/gene="bla"
/note="AmpR promoter"
CDS 1075..1869
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
rep_origin 2045..2633
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 4102..4447
/note="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 4449..4467
/note="MCS 1"
/note="multiple cloning site 1"
intron 4475..5241
/gene="pdk"
/number="3"
/note="PDK intron"
/note="pyruvate orthophosphate dikinase intron from
Flaveria trinervia"
misc_feature 5261..5284
/note="MCS 2"
/note="multiple cloning site 2"
terminator 5288..5994
/note="OCS terminator"
/note="octopine synthase terminator"
ORIGIN
1 tgaccaagtc agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
61 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
121 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggaaat
181 tgtaaacgtt aatattttgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
241 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
301 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
361 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
421 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
481 gggatgcccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
541 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
601 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
661 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
721 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
781 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
841 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
901 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
961 cgttttccaa tgatgagcac tttttgcaag gaacagtgaa ttggagttcg tcttgttata
1021 attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct
1081 aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat
1141 acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat
1201 ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac
1261 atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat
1321 gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat
1381 gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt
1441 cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa
1501 ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac
1561 actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag
1621 gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa
1681 gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc
1741 ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt
1801 gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa
1861 ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc gttccactga
1921 gcgtcagacc ccgtaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
1981 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
2041 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
2101 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
2161 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
2221 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
2281 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
2341 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
2401 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
2461 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
2521 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
2581 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
2641 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
2701 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
2761 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
2821 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
2881 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
2941 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
3001 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgaattt ggccaagtcg
3061 gcctctaata cgactcacta tagggagctc gtcgagcggc cgctcgacga attaattcca
3121 atcccacaaa aatctgagct taacagcaca gttgctcctc tcagagcaga atcgggtatt
3181 caacaccctc atatcaacta ctacgttgtg tataacggtc cacatgccgg tatatacgat
3241 gactggggtt gtacaaaggc ggcaacaaac ggcgttcccg gagttgcaca caagaaattt
3301 gccactatta cagaggcaag agcagcagct gacgcgtaca caacaagtca gcaaacagac
3361 aggttgaact tcatccccaa aggagaagct caactcaagc ccaagagctt tgctaaggcc
3421 ctaacaagcc caccaaagca aaaagcccac tggctcacgc taggaaccaa aaggcccagc
3481 agtgatccag ccccaaaaga gatctccttt gccccggaga ttacaatgga cgatttcctc
3541 tatctttacg atctaggaag gaagttcgaa ggtgaaggtg acgacactat gttcaccact
3601 gataatgaga aggttagcct cttcaatttc agaaagaatg ctgacccaca gatggttaga
3661 gaggcctacg cagcaggtct catcaagacg atctacccga gtaacaatct ccaggagatc
3721 aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ttgcatcaag
3781 aacacagaga aagacatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa
3841 ggcttgcttc ataaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttcct
3901 actgaatcta aggccatgca tggagtctaa gattcaaatc gaggatctaa cagaactcgc
3961 cgtgaagact ggcgaacagt tcatacagag tcttttacga ctcaatgaca agaagaaaat
4021 cttcgtcaac atggtggagc acgacactct ggtctactcc aaaaatgtca aagatacagt
4081 ctcagaagac caaagggcta ttgagacttt tcaacaaagg ataatttcgg gaaacctcct
4141 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaaggtgg
4201 ctcctacaaa tgccatcatt gcgataaagg aaaggctatc attcaagatc tctctgccga
4261 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
4321 aaccacgtct tcaaagcaag tggattgatg tgacatctcc actgacgtaa gggatgacgc
4381 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
4441 gaggacacgc tcgaggaatt cggtacccca attggtaagg aaataattat tttctttttt
4501 ccttttagta taaaatagtt aagtgatgtt aattagtatg attataataa tatagttgtt
4561 ataattgtga aaaaataatt tataaatata ttgtttacat aaacaacata gtaatgtaaa
4621 aaaatatgac aagtgatgtg taagacgaag aagataaaag ttgagagtaa gtatattatt
4681 tttaatgaat ttgatcgaac atgtaagatg atatactagc attaatattt gttttaatca
4741 taatagtaat tctagctggt ttgatgaatt aaatatcaat gataaaatac tatagtaaaa
4801 ataagaataa ataaattaaa ataatatttt tttatgatta atagtttatt atataattaa
4861 atatctatac cattactaaa tattttagtt taaaagttaa taaatatttt gttagaaatt
4921 ccaatctgct tgtaatttat caataaacaa aatattaaat aacaagctaa agtaacaaat
4981 aatatcaaac taatagaaac agtaatctaa tgtaacaaaa cataatctaa tgctaatata
5041 acaaagcgca agatctatca ttttatatag tattattttc aatcaacatt cttattaatt
5101 tctaaataat acttgtagtt ttattaactt ctaaatggat tgactattaa ttaaatgaat
5161 tagtcgaaca tgaataaaca aggtaacatg atagatcatg tcattgtgtt atcattgatc
5221 ttacatttgg attgattaca gttgggaaat tgggttcgaa atcgataagc ttggatcctc
5281 tagagtcctg ctttaatgag atatgcgaga cgcctatgat cgcatgatat ttgctttcaa
5341 ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc tcagatcctt accgccggtt
5401 tcggttcatt ctaatgaata tatcacccgt tactatcgta tttttatgaa taatattctc
5461 cgttcaattt actgattgta ccctactact tatatgtaca atattaaaat gaaaacaata
5521 tattgtgctg aataggttta tagcgacatc tatgatagag cgccacaata acaaacaatt
5581 gcgttttatt attacaaatc caattttaaa aaaagcggca gaaccggtca aacctaaaag
5641 actgattaca taaatcttat tcaaatttca aaaggcccca ggggctagta tctacgacac
5701 accgagcggc gaactaataa cgttcactga agggaactcc ggttccccgc cggcgcgcat
5761 gggtgagatt ccttgaagtt gagtattggc cgtccgctct accgaaagtt acgggcacca
5821 ttcaacccgg tccagcacgg cggccgggta accgacttgc tgccccgaga attatgcagc
5881 atttttttgg tgtatgtggg ccccaaatga agtgcaggtc aaaccttgac agtgacgaca
5941 aatcgttggg cgggtccagg gcgaattttg cgacaacatg tcgaggctca gcaggacctg
6001 caggcatgca agctagctta ctagtgatgc atattctata gtgtcaccta aatctgcggc
6061 cgc
//
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