| 质粒名称: | mScarlet-EEA1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 169067 |
| 存储实验室: | Gia Voeltz |
|
载体骨架基本信息 |
|
| 载体名称: | mScarlet-C1 |
| 载体出品公司: | Clontech |
| 空载体大小: | 4710bp |
| 完整质粒大小: | 8907bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | EEA1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 4236bp |
| 启动子: | CMV |
| 标签/融合蛋白: | mScarlet (N terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | XhoI (not destroyed) |
| 3’克隆位点: | BamHI (not destroyed) |
| 5’测序引物 | CGCAAATGGGCGGTAGGCGTG |
| 3’测序引物 | CAAATGTGGTATGGCTGATT |
|
文献引用方法 |
|
| 材料和方法部分: | mScarlet-EEA1 was a gift from Gia Voeltz (Addgene plasmid # 169067 ; http://n2t.net/addgene:169067 ; RRID:Addgene_169067) |
| 参考文献部分: | Reticulon-3 Promotes Endosome Maturation at ER Membrane Contact Sites. Wu H, Voeltz GK. Dev Cell. 2021 Jan 11;56(1):52-66.e7. doi: 10.1016/j.devcel.2020.12.014. 10.1016/j.devcel.2020.12.014 PubMed 33434526 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS mScarlet-EEA1 8907 bp ds-DNA circular SYN 23-JUL-2021
DEFINITION Express human EEA1 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS mScarlet-EEA1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8907)
AUTHORS Wu H, Voeltz GK
TITLE Reticulon-3 Promotes Endosome Maturation at ER Membrane Contact
Sites.
JOURNAL Dev Cell. 2021 Jan 11;56(1):52-66.e7. doi:
10.1016/j.devcel.2020.12.014.
PUBMED 33434526
REFERENCE 2 (bases 1 to 8907)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 23, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8907
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 98..401
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 402..605
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 556..576
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 644..653
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 650..1345
/codon_start=1
/product="bright monomeric red fluorescent protein evolved
from a synthetic template (Bindels et al., 2016)"
/label=mScarlet
/translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
STGGMDELYK"
primer_bind complement(783..801)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 1051..1070
/label=mCherry-F
/note="mCherry, forward primer"
CDS 1367..5602
/codon_start=1
/product="human early endosome antigen 1"
/label=EEA1
/translation="MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQ
CMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKW
YSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQ
KAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIED
VAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQ
EVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLH
QKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQ
QQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADL
QLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQ
IGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQ
VTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKE
KVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVT
AKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDL
QQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKQIL
KQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKN
SKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLK
LELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQK
TELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQD
LKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQD
IQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQ
LLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEK
EVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEAT
VQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQ
ALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVC
DACFNDLQG"
polyA_signal 5732..5853
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5769..5788)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5823..5842
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(5860..6315)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5997..6018)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6209..6228
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 6342..6446
/gene="bla"
/label=AmpR promoter
promoter 6448..6805
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(6471..6491)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 6656..6791
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6718..6737
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6840..7634
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6894..6913)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7504..7523
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(7822..7841)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 7866..7913
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 8242..8830
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8731..8750
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta
61 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
121 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
181 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
241 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
301 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
361 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
421 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
481 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
541 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
601 gagctggttt agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgagcaa
661 gggcgaggca gtgatcaagg agttcatgcg gttcaaggtg cacatggagg gctccatgaa
721 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
781 cgccaagctg aaggtgacca agggtggccc cctgcccttc tcctgggaca tcctgtcccc
841 tcagttcatg tacggctcca gggccttcac caagcacccc gccgacatcc ccgactacta
901 taagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
961 cgccgtgacc gtgacccagg acacctccct ggaggacggc accctgatct acaaggtgaa
1021 gctccgcggc accaacttcc ctcctgacgg ccccgtaatg cagaagaaga caatgggctg
1081 ggaagcgtcc accgagcggt tgtaccccga ggacggcgtg ctgaagggcg acattaagat
1141 ggccctgcgc ctgaaggacg gcggccgcta cctggcggac ttcaagacca cctacaaggc
1201 caagaagccc gtgcagatgc ccggcgccta caacgtcgac cgcaagttgg acatcacctc
1261 ccacaacgag gactacaccg tggtggaaca gtacgaacgc tccgagggcc gccactccac
1321 cggcggcatg gacgagctgt acaagtccgg actcagatct cgagctatgt taaggaggat
1381 tttacagagg actcctggga gagttggctc tcaaggttct gatttagatt catcagcaac
1441 tcctataaac acagtggacg tcaataatga aagctcttca gagggtttca tatgtcccca
1501 gtgtatgaaa tctcttggat ctgctgatga acttttcaaa cattatgaag ctgttcatga
1561 tgctggtaat gactcaggtc atggaggaga gtctaatctt gctttgaagc gagatgatgt
1621 aacactgctc agacaagagg tccaagacct acaggcttca cttaaggaag aaaaatggta
1681 ctcggaagaa ttaaagaagg aattagaaaa atatcaaggg ctgcagcagc aagaggccaa
1741 acctgatggg ttggtgactg attcatcagc agaactacag tctttggaac agcaattaga
1801 agaagcccaa acagaaaatt ttaatattaa gcaaatgaaa gacttatttg aacagaaagc
1861 agcccaactt gctactgaaa ttgcagatat aaagtcaaag tatgatgaag aaaggagtct
1921 tcgagaagct gctgaacaaa aagtgacacg tctgacagaa gaattaaaca aagaggcaac
1981 tgtaattcaa gatctgaaga cggaactgct tcagagacct ggtatagaag atgttgccgt
2041 gctaaagaaa gaactggtcc aagttcaaac actaatggat aacatgacct tggaacgtga
2101 gcgagaatct gaaaaactca aagatgaatg caaaaaattg cagtcacaat atgctagctc
2161 agaggccaca ataagccagc taaggagtga acttgccaaa ggcccccagg aagttgctgt
2221 atatgtacag gaactacaaa aactgaaaag ttcagttaat gaattaacac aaaaaaatca
2281 gaccttgaca gaaaacttgc tgaaaaaaga acaagactat actaagttag aggagaaaca
2341 taatgaagaa tctgtgagta aaaagaatat tcaggcaacc cttcatcaaa aagacctaga
2401 ttgtcaacag cttcagtcaa gattgtctgc atctgaaacc tcactgcata gaatacatgt
2461 agaactaagt gaaaaaggag aagctactca aaagctcaaa gaagaattat ctgaggtaga
2521 gaccaagtac cagcatctaa aggcggagtt taagcagcta caacaacaga gagaagaaaa
2581 ggagcagcat gggttacaac ttcaaagtga aattaatcaa ttacatagca aacttctgga
2641 gacagagcgc caactagggg aagctcatgg taggctgaag gaacagagac agctttcaag
2701 tgaaaagttg atggataaag aacaacaagt ggctgattta caactcaaac tttctcggtt
2761 agaagagcag ttgaaggaaa aagttacaaa ttctacagaa ttgcagcatc aattagataa
2821 aacaaagcaa cagcatcaag aacaacaggc tcttcagcaa agcaccacgg caaaacttcg
2881 agaagctcag aatgatttgg aacaagttct acgtcaaatt ggcgataagg accaaaagat
2941 ccagaacctt gaagctttat tacagaagag taaagaaaat atttcattac tagaaaaaga
3001 aagagaagat ctttatgcaa aaattcaggc tggtgaagga gagactgctg ttcttaacca
3061 gttacaagaa aaaaaccata cactacagga gcaagtaact caactaacag agaagctgaa
3121 gaatcagtca gaaagtcata aacaagccca ggagaatttg catgaccagg tacaagagca
3181 gaaggcacat cttagagctg cacaagaccg tgtcctttcc ctagaaacta gtgtcaatga
3241 attaaatagt caattaaatg aaagcaagga gaaggtctcc cagcttgaca tacagattaa
3301 agccaaaacc gaactattac tatcagcaga agcagcaaaa actgctcaaa gagctgatct
3361 tcagaatcat ttggacacag ctcaaaatgc attacaagat aaacagcagg agttaaataa
3421 gattactact cagttggatc aggtcactgc aaagttacaa gacaagcaag aacattgcag
3481 tcagctggaa agtcatctta aagaatataa agagaaatac ctctctttag aacagaaaac
3541 cgaagagcta gaaggtcaaa ttaagaaact agaagctgat agtcttgaag ttaaagcaag
3601 caaggagcag gctttgcaag atctacaaca gcaaagacag ctgaacacag atttagagct
3661 cagagccaca gaattgagta aacaacttga aatggagaag gaaatagtat ccagtacaag
3721 attggatcta cagaaaaaat ctgaagccct tgaaagtatc aagcaaaagc ttaccaagca
3781 agaggaagaa aaacaaatcc tgaaacaaga ttttgaaact ttaagtcaag aaacaaagat
3841 tcagcatgag gaattgaata acagaattca aacaacagta acagaactac aaaaagtgaa
3901 aatggagaaa gaagctttaa tgacagagct ttctacagta aaggacaaac tatcaaaagt
3961 ttctgattct ttgaaaaact ctaaaagtga atttgaaaag gagaatcaga aaggaaaagc
4021 cgctatatta gacttggaaa aaacttgcaa agaattaaag catcaacttc aagtgcagat
4081 ggaaaacaca cttaaggaac agaaggaact gaaaaagtca cttgaaaaag agaaggaggc
4141 ttctcatcag ttgaaattgg aactcaattc aatgcaggaa caacttatac aggcccagaa
4201 tactttaaaa caaaatgaaa aagaagagca acaacttcag gggaacataa atgagctaaa
4261 gcaatcaagt gaacagaaga aaaaacaaat tgaagcactc caaggagagc ttaaaattgc
4321 tgttttacag aagacagagc ttgagaataa actacagcag cagttaacac aggcagccca
4381 ggaacttgca gcagagaaag agaaaatatc agtattacaa aacaactatg aaaaaagtca
4441 ggaaactttc aaacagcttc aatctgattt ctatgggagg gaatctgaac ttctagccac
4501 caggcaagat cttaagtctg tagaagagaa gctttctcta gcacaggagg acttgatttc
4561 aaacagaaat caaattggaa atcaaaataa attgattcaa gaactgaaga ctgccaaggc
4621 tacattggag caggattcag caaagaaaga acagcaattg caggagcgat gtaaagcact
4681 acaagacatt cagaaagaaa agtcactgaa agaaaaagaa ctggtaaatg agaagtctaa
4741 attggcagag atagaagaaa ttaaatgtag acaagaaaaa gaaatcacta aactaaacga
4801 agaactcaag tcccacaaac tagaaagcat aaaggagata acaaatctta aagatgctaa
4861 acagcttcta attcagcaga aattagaact tcaaggaaaa gcggactccc tgaaggcagc
4921 tgttgaacag gagaagagaa atcagcagat actaaaagac caggtgaaaa aggaagaaga
4981 ggagctgaag aaagaattta ttgagaaaga agctaagttg cattccgaaa taaaagaaaa
5041 ggaagtagga atgaagaagc atgaagaaaa tgaggctaaa cttaccatgc agattacagc
5101 attaaatgaa aacttaggca ctgtgaagaa ggagtggcaa tctagtcaac ggagagttag
5161 tgagcttgag aaacaaacgg atgacttacg gggtgaaatt gcagtattag aagcaacggt
5221 tcagaataat caagatgaaa ggagagcact actggaaaga tgtcttaaag gagaaggtga
5281 aatagaaaag cttcaaacca aagtattaga attgcaaaga aagctggata atacaactgc
5341 agcagtgcag gagctgggca gagaaaacca atcacttcag atcaaacata cacaagcgtt
5401 gaatagaaag tgggccgaag acaatgaagt acaaaactgt atggcctgtg ggaaaggctt
5461 ttcagtaaca gtgagacggc atcactgccg acagtgtgga aatatcttct gtgctgaatg
5521 ttcagccaaa aatgccttaa ctccttcctc caagaagcct gttcgtgtct gtgatgcatg
5581 tttcaatgac ttgcaaggat aaggatccac cggatctaga taactgatca taatcagcca
5641 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
5701 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
5761 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
5821 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
5881 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
5941 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
6001 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
6061 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
6121 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
6181 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
6241 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
6301 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
6361 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
6421 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
6481 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
6541 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
6601 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
6661 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
6721 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
6781 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
6841 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
6901 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
6961 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
7021 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
7081 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
7141 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
7201 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
7261 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
7321 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
7381 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
7441 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
7501 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
7561 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
7621 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
7681 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
7741 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
7801 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
7861 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
7921 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
7981 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
8041 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
8101 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
8161 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
8221 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
8281 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
8341 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
8401 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
8461 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
8521 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
8581 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
8641 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
8701 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
8761 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
8821 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
8881 tgctcacatg ttctttcctg cgttatc
//
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