pJ241-FLEX

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pJ241-FLEX
出品公司: Addgene
目录编号: 18925
存储实验室: Scott Sternson

载体骨架基本信息
载体名称: pJ241
载体出品公司: DNA2.0
空载体大小: 2791 bp
完整质粒大小: 3063 bp
载体类型: Cre/Lox ; subcloning
载体抗性: Kanamycin
生长温度: 37 ℃
宿主菌: DH5a
拷贝数: 高拷贝

插入基因信息
插入基因名称: FLEX switch / flip-excision switch
物种来源: 光合细菌 Rhodopseudomonas palustris
插入大小: 274 bp
基因突变: An internal Spe1 site is used for subcloning a gene into the FLEX switch.
GenBank编号 EU784738
启动子: CMV
克隆方法: 限制性内切酶
5’克隆位点: BamH1 (not destroyed)
3’克隆位点: BstB1, EcoR1, Pme1, Xba1 (not destroyed)
5’测序引物 n/a

文献引用方法
材料和方法部分:
pJ241-FLEX was a gift from Scott Sternson (Addgene plasmid # 18925 ; http://n2t.net/addgene:18925 ; RRID:Addgene_18925)
 
参考文献部分: A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping. Atasoy D, Aponte Y, Su HH, Sternson SM. J Neurosci. 2008 Jul 9. 28(28):7025-30. 10.1523/JNEUROSCI.1954-08.2008 PubMed 18614669

载体图谱质粒图谱和多克隆位点信息

pJ241-FLEX载体图谱

载体简介

载体序列

LOCUS       pJ241-FLEX                3063 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pJ241-FLEX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Atasoy D, Aponte Y, Su HH, Sternson SM
  TITLE     A FLEX switch targets Channelrhodopsin-2 to multiple cell types for 
            imaging and long-range circuit mapping.
  JOURNAL   J Neurosci. 2008 Jul 9. 28(28):7025-30.
  PUBMED    18614669
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     88..105
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     89..105
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    575..608
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(655..688)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    complement(759..792)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     protein_bind    799..832
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    895..928
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     protein_bind    complement(945..978)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(1185..1204)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        1271..1362
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1363..2172
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2294..2882
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2783..2802
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3036..3053
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgagagtag ggaactgcca ggcatcaaac taagcagaag gcccctgacg gatggccttt
       61 ttgcgtttct acaaactctt tctgtgttgt aaaacgacgg ccagtcttaa gctcgggccc
      121 cctgggcggt tctgataacg agtaatcgtt aatccgcaaa taacgtaaaa acccgcttcg
      181 gcgggttttt ttatgggggg agtttaggga aagagcattt gtcagaatat ttaagggcgc
      241 ctgtcacttt gcttgatata tgagaattat ttaaccttat aaatgagaaa aaagcaacgc
      301 actttaaata agatacgttg ctttttcgat tgatgaacac ctataattaa actattcatc
      361 tattatttat gattttttgt atatacaata tttctagttt gttaaagaga attaagaaaa
      421 taaatctcga aaataataaa gggaaaatca gtttttgata tcaaaattat acatgtcaac
      481 gataatacaa aatataatac aaactataag atgttatcag tatttattat gcatttagaa
      541 taaattttgt gtcgccctta ttcgactcac tatagaagtt cctattctct agaaagtata
      601 ggaacttcac ttcattttct agagtttaaa cgaattcgaa cctcttcgag ggacataact
      661 tcgtataatg tatgctatac gaagttatgg gtcgatggtg agatctggac tagagggtcg
      721 atggtgatgc ttggatcaaa ataggaagaa tcccaatgat aacttcgtat aaagtatcct
      781 atacgaagtt atactagtat aacttcgtat agcatacatt atacgaagtt atgggtcgat
      841 ggtgatgctt ggcaattcgg gtcgatggtg aagcattggt cttcctattt tgatataact
      901 tcgtatagga tactttatac gaagttatgg ggatccccac ttcagaagtt cctatacttt
      961 ctagagaata ggaacttcac tatagagtcg aataagggcg acacccccta attagcccgg
     1021 gcgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     1081 ctctcgcatg gggagtcccc acactaccat cggcgctacg gcgtttcact tctgagttcg
     1141 gcatggggtc aggtgggacc accgcgctac tgccgccagg caaacaaggg gtgttatgag
     1201 ccatattcag gtataaatgg gctcgcgata atgttcagaa ttggttaatt ggttgtaaca
     1261 ctgaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1321 taaccctgat aaatgcttca ataatattga aaaaggaaga atatgagcca tattcaacgg
     1381 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     1441 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     1501 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     1561 atggtcagac taaactggct gacggaattt atgccacttc cgaccatcaa gcattttatc
     1621 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcgttccag
     1681 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     1741 cgccggttgc actcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgc
     1801 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     1861 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     1921 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     1981 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     2041 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     2101 caaaaatatg gtattgataa tcctgatatg aataaattgc aatttcattt gatgctcgat
     2161 gagtttttct aagcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     2221 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     2281 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2341 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2401 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2461 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2521 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2581 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2641 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2701 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2761 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2821 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2881 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2941 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3001 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3061 aag
//

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