出品公司: | Clontech |
---|---|
载体名称: | pLVX-PTuner2-C |
质粒类型: | 慢病毒载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 8537 bp |
5' 测序引物及序列: | CMV Forward: CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | -- |
载体标签: | DD tag (C-端) |
载体抗性: | 氨苄青霉素 |
筛选标记: | 嘌呤霉素(Puromycin) |
克隆菌株: | Stbl3 |
宿主细胞(系): | 常规细胞系(293、CV-1、CHO等) |
备注: |
慢毒载体pLVX-PTuner2-C载体表达N端DD标签融合蛋白; DD标签是一个大小为12KD的FKBP (L106P)去稳定结构域,带有DD标签的目的蛋白将很快被降解; 向培养基中加入Shield1配体后,情况将发生逆转,细胞中目的蛋白的量则迅速提高。 |
产品目录号: | 631098 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 慢病毒 |
买家导航
pLVX-PTuner2-C载体质粒基本信息
pLVX-PTuner2-C质粒图谱载体图谱和pLVX-PTuner2-C载体序列质粒序列多克隆位点信息
pLVX-PTuner2-C质粒载体简介
pLVX-PTuner2-C质粒序列载体序列
LOCUS pLVX-PTuner2-C 8537 bp DNA circular 8-SEP-2010 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Cat. No. 631098,631094 FEATURES Location/Qualifiers LTR 1..635 /label=5'\LTR misc_feature 685..822 /label=Packaging\signal misc_feature 2028..2151 /label=cPPT/CTS\(DNA\flap) promoter 2185..2787 /label=CMV\IE\Promoter misc_feature 4505..5096 /label=WPRE rep_origin complement(6405..7078) /label=pUC\origin CDS complement(7223..8219) /label=Amp(R) misc_binding 1303..1536 /label=RRE misc_binding 636..653 /label=PBS LTR 5299..5935 /label=3'\LTR misc_feature 3363..3871 /label=PGK\Promoter misc_feature 3892..4491 /label=Puro(R) CDS 2868..3341 /label=DD-G misc_feature 2796..2867 /label=MCS BASE COUNT 2134 a 2162 c 2246 g 1995 t ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccatc agtctgattg 2881 cggcgttagc ggtagatcac gttatcggca tggaaaacgc catgccgtgg aacctgcctg 2941 ccgatctcgc ctggtttaaa cgcaacacct taaataaacc cgtgattatg ggccgccata 3001 cctgggaatc aatcggtcgt ccgttgccag gacgcaaaaa tattatcctc agcagtcaac 3061 cgagtacgga cgatcgcgta acgtgggtga agtcggtgga tgaagccatc gcggcgtgtg 3121 gtgacgtacc agaaatcatg gtgattggcg gcggtcgcgt ctatgaacag ctcctgccaa 3181 aagcgcaaaa actgtatctg acgcatatcg acgcagaagt ggaaggcgac acccatttcc 3241 cggattacga gccggatgac tgggaatcgg tattcagcga attccacgat gctgatgcgc 3301 agaactctca cagctattgc tttgagattc tggagcggcg atgagcggcc gcgactctag 3361 ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc 3421 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc 3481 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc 3541 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat 3601 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg 3661 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag 3721 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc 3781 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct 3841 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag 3901 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 3961 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 4021 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 4081 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 4141 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 4201 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 4261 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 4321 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 4381 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 4441 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg 4501 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 4561 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 4621 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 4681 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 4741 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 4801 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 4861 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 4921 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 4981 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 5041 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 5101 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct 5161 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc 5221 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 5281 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt 5341 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg 5401 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca 5461 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg 5521 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca 5581 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct 5641 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg 5701 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct 5761 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 5821 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 5881 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 5941 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 6001 gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat 6061 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 6121 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 6181 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 6241 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 6301 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 6361 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 6421 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 6481 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 6541 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 6601 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 6661 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 6721 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 6781 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 6841 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 6901 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 6961 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 7021 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 7081 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 7141 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 7201 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 7261 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 7321 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 7381 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 7441 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 7501 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 7561 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 7621 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 7681 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 7741 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 7801 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 7861 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 7921 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 7981 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 8041 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 8101 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 8161 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 8221 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc 8281 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 8341 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat 8401 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc 8461 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt 8521 actacaaact tagtagt //
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