pAB675

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相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pAB675
出品公司: Addgene
目录编号: 176578
存储实验室: Arpita Bose

载体骨架基本信息
载体名称: pRhokS_2
载体出品公司: --
空载体大小: --
完整质粒大小: --
载体修饰: --
载体类型: 细菌表达
筛选标记: --
载体抗性: 卡那霉素50 μg/mL
生长温度: 37 ℃
宿主菌: DH5a
拷贝数: 高拷贝

插入基因信息
插入基因名称: phaJ, ter, adhE2
物种来源: --
插入大小: --
基因突变: --
GenBank编号 --
启动子: --
标签/融合蛋白: --
克隆方法: 限制性内切酶
5’克隆位点: --
3’克隆位点: --
5’测序引物 --
3’测序引物 --

文献引用方法
材料和方法部分: pAB675 was a gift from Arpita Bose (Addgene plasmid # 176578 ; http://n2t.net/addgene:176578 ; RRID:Addgene_176578)

载体图谱质粒图谱和多克隆位点信息

pAB675载体图谱

载体简介

载体序列

LOCUS       pAB675              10844 bp ds-DNA     circular SYN 16-NOV-2021
DEFINITION  Expressing three of the five genes of the n-butanol pathway..
ACCESSION   .
VERSION     .
KEYWORDS    pAB675
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10844)
  TITLE     n-butanol pathway plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 16, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10844
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     793..812
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(879..981)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1098..1116)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      2374..3145
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             3146..3808
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     4539..4558
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      4682..4729
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     4725..4743
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(4796..4819)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      4827..4866
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     RBS             9116..9138
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    9153..9177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(9177..9196)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9178..9196)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(9241..10035)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9352..9371)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9962..9981
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
       61 ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta gctcactcat taggcaccgg
      121 gatctcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
      181 gcatgactat aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgcatg
      241 cataaaaact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
      301 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
      361 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
      421 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
      481 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
      541 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
      601 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg
      661 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
      721 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
      781 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
      841 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
      901 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
      961 gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg
     1021 gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt
     1081 tattcgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     1141 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcgccc gcgttcctgc
     1201 tggcgctggg cctgtttctg gcgctggact tcccgctgtt ccgtcagcag cttttcgccc
     1261 acggccttga tgatcgcggc ggccttggcc tgcatatccc gattcaacgg ccccagggcg
     1321 tccagaacgg gcttcaggcg ctcccgaagg tctcgggccg tctcttgggc ttgatcggcc
     1381 ttcttgcgca tctcacgcgc tcctgcggcg gcctgtaggg caggctcata cccctgccga
     1441 accgcttttg tcagccggtc ggccacggct tccggcgtct caacgcgctt tgagattccc
     1501 agcttttcgg ccaatccctg cggtgcatag gcgcgtggct cgaccgcttg cgggctgatg
     1561 gtgacgtggc ccactggtgg ccgctccagg gcctcgtaga acgcctgaat gcgcgtgtga
     1621 cgtgccttgc tgccctcgat gccccgttgc agccctagat cggccacagc ggccgcaaac
     1681 gtggtctggt cgcgggtcat ctgcgctttg ttgccgatga actccttggc cgacagcctg
     1741 ccgtcctgcg tcagcggcac cacgaacgcg gtcatgtgcg ggctggtttc gtcacggtgg
     1801 atgctggccg tcacgatgcg atccgccccg tacttgtccg ccagccactt gtgcgccttc
     1861 tcgaagaacg ccgcctgctg ttcttggctg gccgacttcc accattccgg gctggccgtc
     1921 atgacgtact cgaccgccaa cacagcgtcc ttgcgccgct tctctggcag caactcgcgc
     1981 agtcggccca tcgcttcatc ggtgctgctg gccgcccagt gctcgttctc tggcgtcctg
     2041 ctggcgtcag cgttgggcgt ctcgcgctcg cggtaggcgt gcttgagact ggccgccacg
     2101 ttgcccattt tcgccagctt cttgcatcgc atgatcgcgt atgccgccat gcctgcccct
     2161 cccttttggt gtccaaccgg ctcgacgggg gcagcgcaag gcggtgcctc cggcgggcca
     2221 ctcaatgctt gagtatactc actagacttt gcttcgcaaa gtcgtgaccg cctacggcgg
     2281 ctgcggcgcc ctacgggctt gctctccggg cttcgccctg cgcggtcgct gcgctccctt
     2341 gccagcccgt ggatatgtgg acgatggccg cgagcggcca ccggctggct cgcttcgctc
     2401 ggcccgtgga caaccctgct ggacaagctg atggacaggc tgcgcctgcc cacgagcttg
     2461 accacaggga ttgcccaccg gctacccagc cttcgaccac atacccaccg gctccaactg
     2521 cgcggcctgc ggccttgccc catcaatttt tttaattttc tctggggaaa agcctccggc
     2581 ctgcggcctg cgcgcttcgc ttgccggttg gacaccaagt ggaaggcggg tcaaggctcg
     2641 cgcagcgacc gcgcagcggc ttggccttga cgcgcctgga acgacccaag cctatgcgag
     2701 tgggggcagt cgaagggcga agcccgcccg cctgcccccc gagcctcacg gcggcgagtg
     2761 cgggggttcc aagggggcag cgccaccttg ggcaaggccg aaggccgcgc agtcgatcaa
     2821 caagccccgg aggggccact ttttgccgga gggggagccg cgccgaaggc gtgggggaac
     2881 cccgcagggg tgcccttctt tgggcaccaa agaactagat atagggcgaa atgcgaaaga
     2941 cttaaaaatc aacaacttaa aaaagggggg tacgcaacag ctcattgcgg caccccccgc
     3001 aatagctcat tgcgtaggtt aaagaaaatc tgtaattgac tgccactttt acgcaacgca
     3061 taattgttgt cgcgctgccg aaaagttgca gctgattgcg catggtgccg caaccgtgcg
     3121 gcacccctac cgcatggaga taagcatggc cacgcagtcc agagaaatcg gcattcaagc
     3181 caagaacaag cccggtcact gggtgcaaac ggaacgcaaa gcgcatgagg cgtgggccgg
     3241 gcttattgcg aggaaaccca cggcggcaat gctgctgcat cacctcgtgg cgcagatggg
     3301 ccaccagaac gccgtggtgg tcagccagaa gacactttcc aagctcatcg gacgttcttt
     3361 gcggacggtc caatacgcag tcaaggactt ggtggccgag cgctggatct ccgtcgtgaa
     3421 gctcaacggc cccggcaccg tgtcggccta cgtggtcaat gaccgcgtgg cgtggggcca
     3481 gccccgcgac cagttgcgcc tgtcggtgtt cagtgccgcc gtggtggttg atcacgacga
     3541 ccaggacgaa tcgctgttgg ggcatggcga cctgcgccgc atcccgaccc tgtatccggg
     3601 cgagcagcaa ctaccgaccg gccccggcga ggagccgccc agccagcccg gcattccggg
     3661 catggaacca gacctgccag ccttgaccga aacggaggaa tgggaacggc gcgggcagca
     3721 gcgcctgccg atgcccgatg agccgtgttt tctggacgat ggcgagccgt tggagccgcc
     3781 gacacgggtc acgctgccgc gccggtagca cttgggttgc gcagcaaccc gtaagtgcgc
     3841 tgttccagac tatcggctgt agccgcctcg ccgccctata ccttgtctgc ctccccgcgt
     3901 tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc
     3961 taacggattc accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     4021 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     4081 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     4141 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     4201 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     4261 taaaaaaatt acgccccgcc ctgccactca tcgcagtacg gcctattggt taaaaaatga
     4321 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttccat
     4381 tcgccattca ggctgcgcaa ctgttgggaa gggcgtgaac catcacccta atcaagtttt
     4441 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     4501 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     4561 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     4621 cttaatgcgc cgctacaggg cgcgtcccat tcgccaatcc ggatatagtt cctcctttca
     4681 gcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctagt tattgctcag
     4741 cggtggcagc agccaactca gcttcctttc gggctttgtt agcagccgga tctcactttt
     4801 cgaactgcgg atgcgaccac tgcagctcca aaaaaaaagg ctccaaaagg agcctttaat
     4861 tgtatcggtt tagcatgcat ttagaagctt ttgatgtaga tgtccttcag ctccgagatc
     4921 agcgggtagc gcgggttcgc cgtggtgcac tggtcatcga acgccagctc gctcatcttg
     4981 tcgagggtgt tgtagaagtc cttcttattg atgcccgcgg cggagatatt ctgcgggatc
     5041 gacagatcga tcttgagctt gctgatcgct tcgatcagcg cggtcacctt ttcggtgtcc
     5101 gaggtgccct tcagattcag gtattcggcg atttccgcgt acttgcgctt cgcattcggc
     5161 gacttgtact gcgggaacgc ggtctgcttc gtcgggcagt cggtcgcgtt atacttgatg
     5221 acctcctcga tgagcaccgc gcaggcgatg ccgtgcggca cgtggtgcat ggcgccgagc
     5281 ttatgcgcca tgctgtggca gacgcccagg aaggcgttgg cgaaggccat gccggcgatg
     5341 ttgctggcgt gggccatctt ttcgcgcgct tcgatgtcgt tggtgccgtt tttgtacgcg
     5401 cgcggcaggt atttgaagat catcttgatc gcgcgcagcg cgagttcgtc ggtatagtcg
     5461 gtggccatca ccgacacata cgcctcgatc gcgtggacca gcgcgtcgat gccggtggcg
     5521 gcggtgagct tccgcggcat gttcagcatc agctcggtgt cgatgatcgc catattcggg
     5581 gtcagctcat agctggtcag cgggtacttc atccccgtct cgtcgttggt gatgaccgcg
     5641 aacggggtgg cctccgaccc cgtgccggcg gtggtcggga tcgcgacgga gatcgccttg
     5701 gtgcccagct tcgggaaatt gcagatgcgc ttgcggatgt ccatgaagtt gatggccaga
     5761 ttctcgatct ccgcctccgg atactcgtac agcaggtgca tcaccttggc cgcatccatc
     5821 gggctgccgc cgccgatcga gatgatcgtg tccggctcga agttcagcat ctccttcgcg
     5881 cctttcttca ccgaatcgat ggtcgggtcg gatttgatgt cggtgaagat gctgtacttg
     5941 atgtcgattt cgtcgaggac cttggtgatc ttgttgacgt agcccagctt gaacaggtcc
     6001 ttgtcggtca cgatgaaggc ccgtttcttg ttcatgtcct tgagctcctt cagggcgaag
     6061 cggaggcagc cgtacttgaa gtagatcttc tgcggcacct tgaaccacag catgttttcc
     6121 cggcgttcgg cgacggattt gatgttcagc aggtgttttg gttccacgtt ctgcgagacg
     6181 ctattgccgc cccaggtgcc gcacccgagc gtgaagctcg gcgcgatggc gaaattgtag
     6241 aggtcgcccg aggcgccctg cgagcttggc atgttgatga aggtgcggga ggtcttcatg
     6301 gcgaggccga actctttcac tttatcctta ttgttctggc tgtcgatata cagcgacgag
     6361 gtatggcccg agccgcccag ctcgatcagg cgctgggcct tcttcagggc ttcgtcgaag
     6421 tctttgacct tatacatcgc cagcaccggg ctcagcttct cgtggctgaa cagctccgac
     6481 ttctcgacgg actgcacctc gccgatgagg atcttggtgg tctgcggcac ctcgatgccg
     6541 gccatcttgg cgatgatata ggccgacttg cccacgatgt ccgcattgat cgcgccgttc
     6601 ttaaacatgg tctccttgat cttcgcgatc tcattctggt tcaggatata cgagccgcgc
     6661 ttgacaaact cctccttgac tttctcgtag atgctgttca tgaccaggat gctctgctcg
     6721 ctggcgcaga tcaccccgtt gtcgtaggtc ttggacagga tgatgctcga caccgccatg
     6781 tcgatgtcgg cgctctcgtc gatgatggcc ggggtgttcc ccgcgcccac gccgatcgcg
     6841 ggcttgccgg acgagtacgc cgctttgacc atcgaggggc cgccggtggc gaggatgatg
     6901 tcggcctccg acatgagatc ctggctcagc tcgatcgacg gttcatcgat ccagccgatg
     6961 atgttcttcg gcgcgccggc cttcacggcg gcgtccagga tgagcttggc ggccgcgatg
     7021 gtggacttct tggcgcgcgg atgcgggctg aagaagatcg cgttgcgggt cttcagcgag
     7081 atcaggctct tgaagatggc cgtggaggtc gggttggtgg tggggacgat cgccgcgacg
     7141 atcccgatgg gttccgccac cttggtgatg ccgaggctgt cgtcgtggtc gatgatgccg
     7201 caggtcttct cgttcttgta cttattgtag atgtactccg cggcgaagtg gttcttgatg
     7261 atcttatcct ccaccaggcc gatgccggtc tcctcgacgg ccagcttggc gaggttgatg
     7321 cgctccttgg cggcggcgat ggcgcactgc ttgaagattt tgtcgacctg ctcctgcgtg
     7381 taggtggcga acttcttctg ggcctcccgc agctcattca gcttctgctt cagctccttt
     7441 tggttggtga ccttcatgcg aaacgatcct catcctgaat tcttagatgc ggtcgaagcg
     7501 ctcgacctcc gcctcgtagt tgatgccctc gacatcgaag ccgttgctgg cgaggaagtc
     7561 gtggcggtag ccggccaggt cggtcaggct ctcggcgttc tcgccggtga ccttctccat
     7621 cagcgcgctg accgcctttt ggacgtcctc ctcgagctcc caatcgtcga tccggatccg
     7681 attttcctcg tccaccggga tggtcccgtc cttgcggtac aggcgctcgg cgtacaggcg
     7741 cgtgatctgc tcgatgcagc cttcatggtt gcccttctct ttcatcacct taaacaggga
     7801 cgcgaggtac agcgggatga ccgggatcac ggcgctcgcc cgggtgacca ggcccttgtt
     7861 gaccgagacg aacgcgcgga tggaggggtt ctccttattg aggcggtgcg cggtggcctc
     7921 gaggtgctcc ttggccttgc cgatggtgcc cttccggtag agggcctggg tcgcttccgg
     7981 gccgatatac gagtaggcga gggtgatgca gccctcctcc agcaggccct ccttgctcag
     8041 ctgcttgatc cagcgttccc agtcctcgcc gcccatcacc ttgacggtgg ccgccgcctc
     8101 ctcatcgttg gccggttccg cgctgatctc cttgagctcc ccggtgaacg ggtcgacggt
     8161 cttgcccgtg aaggtcttgc cgaacggctt caggacgctt ttgtgcatga tcccggtatc
     8221 cgggtccgtg cgcaccgggc tcgccagcga gtagacgatc aggtcaaact tgatgccctt
     8281 ctttttggcc tcctcgatga cctgcgcctt gatctcatcc gagaacgcgt cgccatcgat
     8341 ggtcacgctg tacaggccct cgcgcttggc cgcctcgtca aaggccagat tgttgtacca
     8401 gcccggggtg ccgtacttcg tctcgctgcc ggccttctcg aacgagacgc cgatcgtggc
     8461 ggcgccgtag ccgaaggcgg cggtgatgcg cgacgccagg ccatagccgt tcgagcagcc
     8521 gaggacgagg acgttcttcg gggccttcgc gcccgccttg acttccgcgg tgatgcgctt
     8581 cttggtgtac tcgatctggt cctcgacgcc ctttttgcag ccctgcgggt gggcgttcag
     8641 gcagatattg ttgcgcacca tcggcttgac gatcatgcga aacgatcctc atcctgcatg
     8701 catttacggc agcttcacca ccgcttcccc ggtcacggcc agcgcgccgc cctgcgtaaa
     8761 gatgcgggtg gtgagggtgg cgattggctt gtcctcgcgc agcgcggtga cttccacctc
     8821 ggccgtgact tcgtcgccga cgaacaccgg cagcttaaag gacaggctct ggcccaggta
     8881 gatggagcct ttccccggca gctgttggcc gaggaggccg ctgaacaggc tcgcgaggag
     8941 catgccatgc acgatcgggc gttcgaacgc cgtggtcgcc gcaaacgccg ggtccagatg
     9001 cagcgggttg aagtcctcgg agagggccgc gaacgcggcg acttccgccg cgccaaaccg
     9061 cttgctcagg cgcgctttct ggcccacctc cagggactgc gcggacatat gtatatctcc
     9121 ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca caattcccct
     9181 atagtgagtc gtattaattt cgcgggatcg agatcaattc cgcgaacccc agagtcccgc
     9241 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     9301 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     9361 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     9421 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     9481 gacgagatcc tcgccgtcgg gcatccgcgc cttgagcctg gcgaacagtt cggctggcgc
     9541 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     9601 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     9661 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     9721 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     9781 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     9841 cgctgcctcg tcttggagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     9901 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     9961 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
    10021 atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct
    10081 gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac
    10141 cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca
    10201 gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt
    10261 ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg
    10321 actggctttc tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca
    10381 gcgtgaagct agcggaattc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
    10441 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
    10501 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
    10561 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
    10621 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
    10681 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
    10741 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
    10801 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaac
//

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