质粒名称: | pAB675 |
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出品公司: | Addgene |
目录编号: | 176578 |
存储实验室: | Arpita Bose |
载体骨架基本信息 |
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载体名称: | pRhokS_2 |
载体出品公司: | -- |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 细菌表达 |
筛选标记: | -- |
载体抗性: | 卡那霉素50 μg/mL |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 高拷贝 |
插入基因信息 |
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插入基因名称: | phaJ, ter, adhE2 |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子: | -- |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5’克隆位点: | -- |
3’克隆位点: | -- |
5’测序引物 | -- |
3’测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pAB675 was a gift from Arpita Bose (Addgene plasmid # 176578 ; http://n2t.net/addgene:176578 ; RRID:Addgene_176578) |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAB675 10844 bp ds-DNA circular SYN 16-NOV-2021 DEFINITION Expressing three of the five genes of the n-butanol pathway.. ACCESSION . VERSION . KEYWORDS pAB675 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10844) TITLE n-butanol pathway plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 10844) AUTHORS . TITLE Direct Submission JOURNAL Exported Nov 16, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10844 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 793..812 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(879..981) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" primer_bind complement(1098..1116) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" rep_origin 2374..3145 /label=pBBR1 oriV /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication" CDS 3146..3808 /codon_start=1 /product="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" /label=pBBR1 Rep /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR" primer_bind 4539..4558 /label=F1ori-R /note="F1 origin, reverse primer" terminator 4682..4729 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 4725..4743 /label=T7 Term /note="T7 terminator, reverse primer" CDS complement(4796..4819) /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" terminator 4827..4866 /label=fd terminator /note="central terminator from bacteriophage fd (Otsuka and Kunisawa, 1982)" RBS 9116..9138 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind 9153..9177 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind complement(9177..9196) /label=T7 /note="T7 promoter, forward primer" promoter complement(9178..9196) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS complement(9241..10035) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(9352..9371) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 9962..9981 /label=Neo-R /note="Neomycin resistance gene, reverse primer" ORIGIN 1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact 61 ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta gctcactcat taggcaccgg 121 gatctcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg 181 gcatgactat aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgcatg 241 cataaaaact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca 301 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc 361 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg 421 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa 481 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg 541 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg 601 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg 661 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg 721 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta 781 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat 841 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa 901 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg 961 gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg 1021 gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt 1081 tattcgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 1141 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcgccc gcgttcctgc 1201 tggcgctggg cctgtttctg gcgctggact tcccgctgtt ccgtcagcag cttttcgccc 1261 acggccttga tgatcgcggc ggccttggcc tgcatatccc gattcaacgg ccccagggcg 1321 tccagaacgg gcttcaggcg ctcccgaagg tctcgggccg tctcttgggc ttgatcggcc 1381 ttcttgcgca tctcacgcgc tcctgcggcg gcctgtaggg caggctcata cccctgccga 1441 accgcttttg tcagccggtc ggccacggct tccggcgtct caacgcgctt tgagattccc 1501 agcttttcgg ccaatccctg cggtgcatag gcgcgtggct cgaccgcttg cgggctgatg 1561 gtgacgtggc ccactggtgg ccgctccagg gcctcgtaga acgcctgaat gcgcgtgtga 1621 cgtgccttgc tgccctcgat gccccgttgc agccctagat cggccacagc ggccgcaaac 1681 gtggtctggt cgcgggtcat ctgcgctttg ttgccgatga actccttggc cgacagcctg 1741 ccgtcctgcg tcagcggcac cacgaacgcg gtcatgtgcg ggctggtttc gtcacggtgg 1801 atgctggccg tcacgatgcg atccgccccg tacttgtccg ccagccactt gtgcgccttc 1861 tcgaagaacg ccgcctgctg ttcttggctg gccgacttcc accattccgg gctggccgtc 1921 atgacgtact cgaccgccaa cacagcgtcc ttgcgccgct tctctggcag caactcgcgc 1981 agtcggccca tcgcttcatc ggtgctgctg gccgcccagt gctcgttctc tggcgtcctg 2041 ctggcgtcag cgttgggcgt ctcgcgctcg cggtaggcgt gcttgagact ggccgccacg 2101 ttgcccattt tcgccagctt cttgcatcgc atgatcgcgt atgccgccat gcctgcccct 2161 cccttttggt gtccaaccgg ctcgacgggg gcagcgcaag gcggtgcctc cggcgggcca 2221 ctcaatgctt gagtatactc actagacttt gcttcgcaaa gtcgtgaccg cctacggcgg 2281 ctgcggcgcc ctacgggctt gctctccggg cttcgccctg cgcggtcgct gcgctccctt 2341 gccagcccgt ggatatgtgg acgatggccg cgagcggcca ccggctggct cgcttcgctc 2401 ggcccgtgga caaccctgct ggacaagctg atggacaggc tgcgcctgcc cacgagcttg 2461 accacaggga ttgcccaccg gctacccagc cttcgaccac atacccaccg gctccaactg 2521 cgcggcctgc ggccttgccc catcaatttt tttaattttc tctggggaaa agcctccggc 2581 ctgcggcctg cgcgcttcgc ttgccggttg gacaccaagt ggaaggcggg tcaaggctcg 2641 cgcagcgacc gcgcagcggc ttggccttga cgcgcctgga acgacccaag cctatgcgag 2701 tgggggcagt cgaagggcga agcccgcccg cctgcccccc gagcctcacg gcggcgagtg 2761 cgggggttcc aagggggcag cgccaccttg ggcaaggccg aaggccgcgc agtcgatcaa 2821 caagccccgg aggggccact ttttgccgga gggggagccg cgccgaaggc gtgggggaac 2881 cccgcagggg tgcccttctt tgggcaccaa agaactagat atagggcgaa atgcgaaaga 2941 cttaaaaatc aacaacttaa aaaagggggg tacgcaacag ctcattgcgg caccccccgc 3001 aatagctcat tgcgtaggtt aaagaaaatc tgtaattgac tgccactttt acgcaacgca 3061 taattgttgt cgcgctgccg aaaagttgca gctgattgcg catggtgccg caaccgtgcg 3121 gcacccctac cgcatggaga taagcatggc cacgcagtcc agagaaatcg gcattcaagc 3181 caagaacaag cccggtcact gggtgcaaac ggaacgcaaa gcgcatgagg cgtgggccgg 3241 gcttattgcg aggaaaccca cggcggcaat gctgctgcat cacctcgtgg cgcagatggg 3301 ccaccagaac gccgtggtgg tcagccagaa gacactttcc aagctcatcg gacgttcttt 3361 gcggacggtc caatacgcag tcaaggactt ggtggccgag cgctggatct ccgtcgtgaa 3421 gctcaacggc cccggcaccg tgtcggccta cgtggtcaat gaccgcgtgg cgtggggcca 3481 gccccgcgac cagttgcgcc tgtcggtgtt cagtgccgcc gtggtggttg atcacgacga 3541 ccaggacgaa tcgctgttgg ggcatggcga cctgcgccgc atcccgaccc tgtatccggg 3601 cgagcagcaa ctaccgaccg gccccggcga ggagccgccc agccagcccg gcattccggg 3661 catggaacca gacctgccag ccttgaccga aacggaggaa tgggaacggc gcgggcagca 3721 gcgcctgccg atgcccgatg agccgtgttt tctggacgat ggcgagccgt tggagccgcc 3781 gacacgggtc acgctgccgc gccggtagca cttgggttgc gcagcaaccc gtaagtgcgc 3841 tgttccagac tatcggctgt agccgcctcg ccgccctata ccttgtctgc ctccccgcgt 3901 tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc 3961 taacggattc accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat 4021 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt 4081 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta 4141 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc 4201 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct 4261 taaaaaaatt acgccccgcc ctgccactca tcgcagtacg gcctattggt taaaaaatga 4321 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttccat 4381 tcgccattca ggctgcgcaa ctgttgggaa gggcgtgaac catcacccta atcaagtttt 4441 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga 4501 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 4561 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg 4621 cttaatgcgc cgctacaggg cgcgtcccat tcgccaatcc ggatatagtt cctcctttca 4681 gcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctagt tattgctcag 4741 cggtggcagc agccaactca gcttcctttc gggctttgtt agcagccgga tctcactttt 4801 cgaactgcgg atgcgaccac tgcagctcca aaaaaaaagg ctccaaaagg agcctttaat 4861 tgtatcggtt tagcatgcat ttagaagctt ttgatgtaga tgtccttcag ctccgagatc 4921 agcgggtagc gcgggttcgc cgtggtgcac tggtcatcga acgccagctc gctcatcttg 4981 tcgagggtgt tgtagaagtc cttcttattg atgcccgcgg cggagatatt ctgcgggatc 5041 gacagatcga tcttgagctt gctgatcgct tcgatcagcg cggtcacctt ttcggtgtcc 5101 gaggtgccct tcagattcag gtattcggcg atttccgcgt acttgcgctt cgcattcggc 5161 gacttgtact gcgggaacgc ggtctgcttc gtcgggcagt cggtcgcgtt atacttgatg 5221 acctcctcga tgagcaccgc gcaggcgatg ccgtgcggca cgtggtgcat ggcgccgagc 5281 ttatgcgcca tgctgtggca gacgcccagg aaggcgttgg cgaaggccat gccggcgatg 5341 ttgctggcgt gggccatctt ttcgcgcgct tcgatgtcgt tggtgccgtt tttgtacgcg 5401 cgcggcaggt atttgaagat catcttgatc gcgcgcagcg cgagttcgtc ggtatagtcg 5461 gtggccatca ccgacacata cgcctcgatc gcgtggacca gcgcgtcgat gccggtggcg 5521 gcggtgagct tccgcggcat gttcagcatc agctcggtgt cgatgatcgc catattcggg 5581 gtcagctcat agctggtcag cgggtacttc atccccgtct cgtcgttggt gatgaccgcg 5641 aacggggtgg cctccgaccc cgtgccggcg gtggtcggga tcgcgacgga gatcgccttg 5701 gtgcccagct tcgggaaatt gcagatgcgc ttgcggatgt ccatgaagtt gatggccaga 5761 ttctcgatct ccgcctccgg atactcgtac agcaggtgca tcaccttggc cgcatccatc 5821 gggctgccgc cgccgatcga gatgatcgtg tccggctcga agttcagcat ctccttcgcg 5881 cctttcttca ccgaatcgat ggtcgggtcg gatttgatgt cggtgaagat gctgtacttg 5941 atgtcgattt cgtcgaggac cttggtgatc ttgttgacgt agcccagctt gaacaggtcc 6001 ttgtcggtca cgatgaaggc ccgtttcttg ttcatgtcct tgagctcctt cagggcgaag 6061 cggaggcagc cgtacttgaa gtagatcttc tgcggcacct tgaaccacag catgttttcc 6121 cggcgttcgg cgacggattt gatgttcagc aggtgttttg gttccacgtt ctgcgagacg 6181 ctattgccgc cccaggtgcc gcacccgagc gtgaagctcg gcgcgatggc gaaattgtag 6241 aggtcgcccg aggcgccctg cgagcttggc atgttgatga aggtgcggga ggtcttcatg 6301 gcgaggccga actctttcac tttatcctta ttgttctggc tgtcgatata cagcgacgag 6361 gtatggcccg agccgcccag ctcgatcagg cgctgggcct tcttcagggc ttcgtcgaag 6421 tctttgacct tatacatcgc cagcaccggg ctcagcttct cgtggctgaa cagctccgac 6481 ttctcgacgg actgcacctc gccgatgagg atcttggtgg tctgcggcac ctcgatgccg 6541 gccatcttgg cgatgatata ggccgacttg cccacgatgt ccgcattgat cgcgccgttc 6601 ttaaacatgg tctccttgat cttcgcgatc tcattctggt tcaggatata cgagccgcgc 6661 ttgacaaact cctccttgac tttctcgtag atgctgttca tgaccaggat gctctgctcg 6721 ctggcgcaga tcaccccgtt gtcgtaggtc ttggacagga tgatgctcga caccgccatg 6781 tcgatgtcgg cgctctcgtc gatgatggcc ggggtgttcc ccgcgcccac gccgatcgcg 6841 ggcttgccgg acgagtacgc cgctttgacc atcgaggggc cgccggtggc gaggatgatg 6901 tcggcctccg acatgagatc ctggctcagc tcgatcgacg gttcatcgat ccagccgatg 6961 atgttcttcg gcgcgccggc cttcacggcg gcgtccagga tgagcttggc ggccgcgatg 7021 gtggacttct tggcgcgcgg atgcgggctg aagaagatcg cgttgcgggt cttcagcgag 7081 atcaggctct tgaagatggc cgtggaggtc gggttggtgg tggggacgat cgccgcgacg 7141 atcccgatgg gttccgccac cttggtgatg ccgaggctgt cgtcgtggtc gatgatgccg 7201 caggtcttct cgttcttgta cttattgtag atgtactccg cggcgaagtg gttcttgatg 7261 atcttatcct ccaccaggcc gatgccggtc tcctcgacgg ccagcttggc gaggttgatg 7321 cgctccttgg cggcggcgat ggcgcactgc ttgaagattt tgtcgacctg ctcctgcgtg 7381 taggtggcga acttcttctg ggcctcccgc agctcattca gcttctgctt cagctccttt 7441 tggttggtga ccttcatgcg aaacgatcct catcctgaat tcttagatgc ggtcgaagcg 7501 ctcgacctcc gcctcgtagt tgatgccctc gacatcgaag ccgttgctgg cgaggaagtc 7561 gtggcggtag ccggccaggt cggtcaggct ctcggcgttc tcgccggtga ccttctccat 7621 cagcgcgctg accgcctttt ggacgtcctc ctcgagctcc caatcgtcga tccggatccg 7681 attttcctcg tccaccggga tggtcccgtc cttgcggtac aggcgctcgg cgtacaggcg 7741 cgtgatctgc tcgatgcagc cttcatggtt gcccttctct ttcatcacct taaacaggga 7801 cgcgaggtac agcgggatga ccgggatcac ggcgctcgcc cgggtgacca ggcccttgtt 7861 gaccgagacg aacgcgcgga tggaggggtt ctccttattg aggcggtgcg cggtggcctc 7921 gaggtgctcc ttggccttgc cgatggtgcc cttccggtag agggcctggg tcgcttccgg 7981 gccgatatac gagtaggcga gggtgatgca gccctcctcc agcaggccct ccttgctcag 8041 ctgcttgatc cagcgttccc agtcctcgcc gcccatcacc ttgacggtgg ccgccgcctc 8101 ctcatcgttg gccggttccg cgctgatctc cttgagctcc ccggtgaacg ggtcgacggt 8161 cttgcccgtg aaggtcttgc cgaacggctt caggacgctt ttgtgcatga tcccggtatc 8221 cgggtccgtg cgcaccgggc tcgccagcga gtagacgatc aggtcaaact tgatgccctt 8281 ctttttggcc tcctcgatga cctgcgcctt gatctcatcc gagaacgcgt cgccatcgat 8341 ggtcacgctg tacaggccct cgcgcttggc cgcctcgtca aaggccagat tgttgtacca 8401 gcccggggtg ccgtacttcg tctcgctgcc ggccttctcg aacgagacgc cgatcgtggc 8461 ggcgccgtag ccgaaggcgg cggtgatgcg cgacgccagg ccatagccgt tcgagcagcc 8521 gaggacgagg acgttcttcg gggccttcgc gcccgccttg acttccgcgg tgatgcgctt 8581 cttggtgtac tcgatctggt cctcgacgcc ctttttgcag ccctgcgggt gggcgttcag 8641 gcagatattg ttgcgcacca tcggcttgac gatcatgcga aacgatcctc atcctgcatg 8701 catttacggc agcttcacca ccgcttcccc ggtcacggcc agcgcgccgc cctgcgtaaa 8761 gatgcgggtg gtgagggtgg cgattggctt gtcctcgcgc agcgcggtga cttccacctc 8821 ggccgtgact tcgtcgccga cgaacaccgg cagcttaaag gacaggctct ggcccaggta 8881 gatggagcct ttccccggca gctgttggcc gaggaggccg ctgaacaggc tcgcgaggag 8941 catgccatgc acgatcgggc gttcgaacgc cgtggtcgcc gcaaacgccg ggtccagatg 9001 cagcgggttg aagtcctcgg agagggccgc gaacgcggcg acttccgccg cgccaaaccg 9061 cttgctcagg cgcgctttct ggcccacctc cagggactgc gcggacatat gtatatctcc 9121 ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca caattcccct 9181 atagtgagtc gtattaattt cgcgggatcg agatcaattc cgcgaacccc agagtcccgc 9241 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat 9301 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg 9361 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa 9421 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac 9481 gacgagatcc tcgccgtcgg gcatccgcgc cttgagcctg gcgaacagtt cggctggcgc 9541 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt 9601 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag 9661 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg 9721 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc 9781 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg 9841 cgctgcctcg tcttggagtt cattcagggc accggacagg tcggtcttga caaaaagaac 9901 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg 9961 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc 10021 atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct 10081 gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac 10141 cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca 10201 gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt 10261 ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg 10321 actggctttc tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca 10381 gcgtgaagct agcggaattc attaagttct gtctcggcgc gtctgcgtct ggctggctgg 10441 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt 10501 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg 10561 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg 10621 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt 10681 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg 10741 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc 10801 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaac //
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