| 质粒名称: | pAd-M3cherry |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 61041 |
| 存储实验室: | Qiao Zhou |
|
载体骨架基本信息 |
|
| 载体名称: | pAd CMV/V5-DEST |
| 载体出品公司: | lifetechnologies |
| 空载体大小: | 36686 bp |
| 完整质粒大小: | 40200 bp |
| 载体类型: | Adenoviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称1: | Ngn3 / Neurog3 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 642 bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | salI (not destroyed) |
| 3’克隆位点: | SpeI (not destroyed) |
| 5’测序引物 | ATGGCGCCTCATCCCT |
| 3’测序引物 | GTTC TCAGACTTCTTG |
| 插入基因名称2: | pdx1 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 852bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI (not destroyed) |
| 3’克隆位点: | BamHI (not destroyed) |
| 5’测序引物 | ATGAACAGTGAGGAG |
| 3’测序引物 | CAGGAACCCCGG |
| 插入基因名称3: | Mafa |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 1077bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | ClaI (not destroyed) |
| 3’克隆位点: | ClaI (not destroyed) |
| 5’测序引物 | ATGGCCGCGGAGC |
| CACCCGACTTCTTTCTG | |
| 插入基因名称4: | monomeric cherry |
| 物种来源: | Synthetic |
| 插入大小: | 711bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | NotI (not destroyed) |
| 3’克隆位点: | NotI (not destroyed) |
| 5’测序引物 | atggtgagcaagggcgagg |
| 3’测序引物 | ggacgagctgtacaagtaa |
|
文献引用方法 |
|
| 材料和方法部分: |
pAd-M3cherry was a gift from Qiao Zhou (Addgene plasmid # 61041 ; http://n2t.net/addgene:61041 ; RRID:Addgene_61041)
|
| 参考文献部分: | Long-term persistence and development of induced pancreatic beta cells generated by lineage conversion of acinar cells. Li W, Cavelti-Weder C, Zhang Y, Clement K, Donovan S, Gonzalez G, Zhu J, Stemann M, Xu K, Hashimoto T, Yamada T, Nakanishi M, Zhang Y, Zeng S, Gifford D, Meissner A, Weir G, Zhou Q. Nat Biotechnol. 2014 Dec;32(12):1223-30. doi: 10.1038/nbt.3082. Epub 2014 Nov 17. 10.1038/nbt.3082 PubMed 25402613 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAd-M3cherry 860 bp DNA linear UNA 08-JUL-2024
DEFINITION natural linear DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE natural DNA sequence
ORGANISM unspecified
REFERENCE 1 (bases 1 to 860)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 8, 2024 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..860
/mol_type="genomic DNA"
/organism="unspecified"
ORIGIN
1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
61 taatacgact cactataggg agacccaagc tggctagtta agctatcaac aagtttgtac
121 aaaaaagcag gctggcgccg gaaccaattc agtcgacgcc gccaccatgg cgcctcatcc
181 cttggatgcg ctcaccatcc aagtgtcccc agagacacaa caaccttttc ccggagcctc
241 ggaccacgaa gtgctcagtt ccaattccac cccacctagc cccactctca tacctaggga
301 ctgctccgaa gcagaagtgg gtgactgccg agggacctcg aggaagctcc gcgcccgacg
361 cggagggcgc aacaggccca agagcgagtt ggcactcagc aaacagcgaa gaagccggcg
421 caagaaggcc aatgatcggg agcgcaatcg catgcacaac ctcaactcgg cgctggatgc
481 gctgcgcggt gtcctgccca ccttcccgga tgacgccaaa cttacaaaga tcgagaccct
541 gcgcttcgcc cacaactaca tctgggcact gactcagacg ctgcgcatag cggaccacag
601 cttctatggc ccggagcccc ctgtgccctg tggagagctg gggagccccg gaggtggctc
661 caacggggac tggggctcta tctactcccc agtctcccaa gcgggtaacc tgagccccac
721 ggcctcattg gaggaattcc ctggcctgca ggtgcccagc tccccatcct atctgctccc
781 gggagcactg gtgttctcag acttcttgac tagtgccacg aacttctctc tgttaaagca
841 agcaggagat gttgaagaaa
//
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