pUC18-mini-Tn7T-Gm-lux

LOCUS       pUC18-mini-Tn7T-Gm-lux              11299 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  mini-Tn7 luxCDABE transcriptional fusion vector.
ACCESSION   .
VERSION     .
KEYWORDS    pUC18-mini-Tn7T-Gm-lux
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11299)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 11299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11299
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     584..600
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      629..723
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    958..1005
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1135..1668)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(1857..1885)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    1926..1973
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2517..3959
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             3971..4894
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             4943..6025
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             6040..7023
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             7202..8314
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     mobile_element  9045..9210
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     primer_bind     complement(9309..9326)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9480..10068)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9560..9579)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10239..11099)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10862..10881
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11100..11204)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11272..11290
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg ggatcttgaa
      961 gtacctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
     1021 ctgatgtgct taaaaactta ctcaatggaa ttctagatcg aattggccgc ggcgttgtga
     1081 caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt
     1141 ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata
     1201 gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag
     1261 cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg
     1321 ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct caaacttggg
     1381 cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat
     1441 gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta
     1501 ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac
     1561 ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt
     1621 tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc
     1681 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag
     1741 actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     1801 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt
     1861 tacgaaccga acaggcttat gtcaattcga tccattgctg ttgacaaagg gaatcagggg
     1921 atcttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
     1981 ttttgaagct aattcgatca tgcatgagct cactagtgga tcccccgggc tgcaggaatt
     2041 cctcgagaag ctgatcccca attcttcttt agaaatctgc cggtaaaaat tagattgcta
     2101 ttcaatctat ttctatcggt atttgtgaaa taatactcag gataataatt tacataaata
     2161 ttatcacgca ttagagaaga gcatgacttt tttaatttaa acttttcatt aacaaatctt
     2221 gttgatatga aaattttcct ttgctatttt aacagatatt aaaacgggaa taggcgttat
     2281 attgacgatc cattcagtta gattaaaaac cttgagcaga aaatttatat tattatcata
     2341 attatgacga aagttacagg ccaggaacca cgtagtcaga atctgatttt ctatatattt
     2401 gttatttaca tcgtcataac acaaaaatat aagaagcaag tgttggtacg accagttcgc
     2461 aagatagtta aacagcaact taagttgaaa ttacccccat taaatggatg gcaaatatga
     2521 ctaaaaaaat ttcattcatt attaacggcc aggttgaaat ctttcccgaa agtgatgatt
     2581 tagtgcaatc cattaatttt ggtgataata gtgtttacct gccaatattg aatgactctc
     2641 atgtaaaaaa cattattgat tgtaatggaa ataacgaatt acggttgcat aacattgtca
     2701 attttctcta tacggtaggg caaagatgga aaaatgaaga atactcaaga cgcaggacat
     2761 acattcgtga cttaaaaaaa tatatgggat attcagaaga aatggctaag ctagaggcca
     2821 attggatatc tatgatttta tgttctaaag gcggccttta tgatgttgta gaaaatgaac
     2881 ttggttctcg ccatatcatg gatgaatggc tacctcagga tgaaagttat gttcgggctt
     2941 ttccgaaagg taaatctgta catctgttgg caggtaatgt tccattatct gggatcatgt
     3001 ctatattacg cgcaatttta actaagaatc agtgtattat aaaaacatcg tcaaccgatc
     3061 cttttaccgc taatgcatta gcgttaagtt ttattgatgt agaccctaat catccgataa
     3121 cgcgctcttt atctgttata tattggcccc accaaggtga tacatcactc gcaaaagaaa
     3181 ttatgcgaca tgcggatgtt attgtcgctt ggggagggcc agatgcgatt aattgggcgg
     3241 tagagcacgc gccatcttat gctgatgtga ttaaatttgg ttctaaaaag agtctttgca
     3301 ttatcgataa tcctgttgat ttgacgtccg cagcgacagg tgcggctcat gatgtttgtt
     3361 tttacgatca gcgagcttgt ttttctgccc aaaacatata ttacatggga aatcattatg
     3421 aggaatttaa gttagcgttg atagaaaaac ttaatctata tgcgcatata ttaccgaatg
     3481 ccaaaaaaga ttttgatgaa aaggcggcct attctttagt tcaaaaagaa agcctgtttg
     3541 ctggattaaa agtagaggtg gatattcatc aacgttggat gattattgag tcaaatgcag
     3601 gtgtggaatt taatcaacca cttggcagat gtgtgtacct tcatcacgtc gataatattg
     3661 agcaaatatt gccttatgtt caaaaaaata agacgcaaac catatctatt tttccttggg
     3721 agtcatcatt taaatatcga gatgcgttag cattaaaagg tgcggaaagg attgtagaag
     3781 caggaatgaa taacatattt cgagttggtg gatctcatga cggaatgaga ccgttgcaac
     3841 gattagtgac atatatttct catgaaaggc catctaacta tacggctaag gatgttgcgg
     3901 ttgaaataga acagactcga ttcctggaag aagataagtt ccttgtattt gtcccataat
     3961 aggtaaaagt atggaaaatg aatcaaaata taaaaccatc gaccacgtta tttgtgttga
     4021 aggaaataaa aaaattcatg tttgggaaac gctgccagaa gaaaacagcc caaagagaaa
     4081 gaatgccatt attattgcgt ctggttttgc ccgcaggatg gatcattttg ctggtctggc
     4141 ggaatattta tcgcggaatg gatttcatgt gatccgctat gattcgcttc accacgttgg
     4201 attgagttca gggacaattg atgaatttac aatgtctata ggaaagcaga gcttgttagc
     4261 agtggttgat tggttaacta cacgaaaaat aaataacttc ggtatgttgg cttccagctt
     4321 atctgcgcgg atagcttatg caagcctatc tgaaatcaat gcttcgtttt taatcaccgc
     4381 agtcggtgtt gttaacttaa gatattctct tgaaagagct ttagggtttg attatctcag
     4441 tctacccatt aatgaattgc cggataatct ggattttgaa ggccataaat tgggtgctga
     4501 agtctttgcg agagattgtc ttgattttgg ttgggaagat ttagcttcta caattaataa
     4561 catgatgtat cttgatatac cgtttattgc ttttactgca aataacgata attgggtcaa
     4621 gcaagatgaa gttatcacat tgttatcaaa tattcgtagt aatcgatgca agatatattc
     4681 tttgttagga agttcgcatg acttgagtga aaatttagtg gtcctgcgca atttttatca
     4741 atcggttacg aaagccgcta tcgcgatgga taatgatcat ctggatattg atgttgatat
     4801 tactgaaccg tcatttgaac atttaactat tgcgacagtc aatgaacgcc gaatgagaat
     4861 tgagattgaa aatcaagcaa tttctctgtc ttaaaatcta ttgagatatt ctatcactca
     4921 aatagcaata taaggactct ctatgaaatt tggaaacttt ttgcttacat accaacctcc
     4981 ccaattttct caaacagagg taatgaaacg tttggttaaa ttaggtcgca tctctgagga
     5041 gtgtggtttt gataccgtat ggttactgga gcatcatttc acggagtttg gtttgcttgg
     5101 taacccttat gtcgctgctg catatttact tggcgcgact aaaaaattga atgtaggaac
     5161 tgccgctatt gttcttccca cagcccatcc agtacgccaa cttgaagatg tgaatttatt
     5221 ggatcaaatg tcaaaaggac gatttcggtt tggtatttgc cgagggcttt acaacaagga
     5281 ctttcgcgta ttcggcacag atatgaataa cagtcgcgcc ttagcggaat gctggtacgg
     5341 gctgataaag aatggcatga cagagggata tatggaagct gataatgaac atatcaagtt
     5401 ccataaggta aaagtaaacc ccgcggcgta tagcagaggt ggcgcaccgg tttatgtggt
     5461 ggctgaatca gcttcgacga ctgagtgggc tgctcaattt ggcctaccga tgatattaag
     5521 ttggattata aatactaacg aaaagaaagc acaacttgag ctttataatg aagtggctca
     5581 agaatatggg cacgatattc ataatatcga ccattgctta tcatatataa catctgtaga
     5641 tcatgactca attaaagcga aagagatttg ccggaaattt ctggggcatt ggtatgattc
     5701 ttatgtgaat gctacgacta tttttgatga ttcagaccaa acaagaggtt atgatttcaa
     5761 taaagggcag tggcgtgact ttgtattaaa aggacataaa gatactaatc gccgtattga
     5821 ttacagttac gaaatcaatc ccgtgggaac gccgcaggaa tgtattgaca taattcaaaa
     5881 agacattgat gctacaggaa tatcaaatat ttgttgtgga tttgaagcta atggaacagt
     5941 agacgaaatt attgcttcca tgaagctctt ccagtctgat gtcatgccat ttcttaaaga
     6001 aaaacaacgt tcgctattat attagctaag gagaaagaaa tgaaatttgg attgttcttc
     6061 cttaacttca tcaattcaac aactgttcaa gaacaaagta tagttcgtat gcaggaaata
     6121 acggagtatg ttgataagtt gaattttgaa cagattttag tgtatgaaaa tcatttttca
     6181 gataatggtg ttgtcggcgc tcctctgact gtttctggtt ttctgctcgg tttaacagag
     6241 aaaattaaaa ttggttcatt aaatcacatc attacaactc atcatcctgt cgccatagcg
     6301 gaggaagcgt gcttattgga tcagttaagt gaagggagat ttattttagg gtttagtgat
     6361 tgcgaaaaaa aagatgaaat gcattttttt aatcgcccgg ttgaatatca acagcaacta
     6421 tttgaagagt gttatgaaat cattaacgat gctttaacaa caggctattg taatccagat
     6481 aacgattttt atagcttccc taaaatatct gtaaatcccc atgcttatac gccaggcgga
     6541 cctcggaaat atgtaacagc aaccagtcat catattgttg agtgggcggc caaaaaaggt
     6601 attcctctca tctttaagtg ggatgattct aatgatgtta gatatgaata tgctgaaaga
     6661 tataaagccg ttgcggataa atatgacgtt gacctatcag agatagacca tcagttaatg
     6721 atattagtta actataacga agatagtaat aaagctaaac aagagacgcg tgcatttatt
     6781 agtgattatg ttcttgaaat gcaccctaat gaaaatttcg aaaataaact tgaagaaata
     6841 attgcagaaa acgctgtcgg aaattatacg gagtgtataa ctgcggctaa gttggcaatt
     6901 gaaaagtgtg gtgcgaaaag tgtattgctg tcctttgaac caatgaatga tttgatgagc
     6961 caaaaaaatg taatcaatat tgttgatgat aatattaaga agtaccacat ggaatatacc
     7021 taatagattt cgagttgcag cgaggcggca agtgaacgaa tccccaggag catagataac
     7081 tatgtgactg gggtgagtga aagcagccaa caaagcagca gcttgaaaga tgaagggtat
     7141 aaaagagtat gacagcagtg ctgccatact ttctaatatt atcttgagga gtaaaacagg
     7201 tatgacttca tatgttgata aacaagaaat tacagcaagc tcagaaattg atgatttgat
     7261 tttttcgagc gatccattag tgtggtctta cgacgagcag gaaaaaatca gaaagaaact
     7321 tgtgcttgat gcatttcgta atcattataa acattgtcga gaatatcgtc actactgtca
     7381 ggcacacaaa gtagatgaca atattacgga aattgatgac atacctgtat tcccaacatc
     7441 ggtttttaag tttactcgct tattaacttc tcaggaaaac gagattgaaa gttggtttac
     7501 cagtagcggc acgaatggtt taaaaagtca ggtggcgcgt gacagattaa gtattgagag
     7561 actcttaggc tctgtgagtt atggcatgaa atatgttggt agttggtttg atcatcaaat
     7621 agaattagtc aatttgggac cagatagatt taatgctcat aatatttggt ttaaatatgt
     7681 tatgagtttg gtggaattgt tatatcctac gacatttacc gtaacagaag aacgaataga
     7741 ttttgttaaa acattgaata gtcttgaacg aataaaaaat caagggaaag atctttgtct
     7801 tattggttcg ccatacttta tttatttact ctgccattat atgaaagata aaaaaatctc
     7861 attttctgga gataaaagcc tttatatcat aaccggaggc ggctggaaaa gttacgaaaa
     7921 agaatctctg aaacgtgatg atttcaatca tcttttattt gatactttca atctcagtga
     7981 tattagtcag atccgagata tatttaatca agttgaactc aacacttgtt tctttgagga
     8041 tgaaatgcag cgtaaacatg ttccgccgtg ggtatatgcg cgagcgcttg atcctgaaac
     8101 gttgaaacct gtacctgatg gaacgccggg gttgatgagt tatatggatg cgtcagcaac
     8161 cagttatcca gcatttattg ttaccgatga tgtcgggata attagcagag aatatggtaa
     8221 gtatcccggc gtgctcgttg aaattttacg tcgcgtcaat acgaggacgc agaaagggtg
     8281 tgctttgagc ttaaccgaag cgtttgatag ttgatatcct ttgcctaatt gtaagtggaa
     8341 tgcttgcgtt atataaatct gaatgacatc tacactttac aaaattctcc aaaacatcca
     8401 catttgggta cttgatagag gtttatgggg ttggcttaac attgttctca ttgttattat
     8461 tggctcaaag caaaaggaga taacatgaaa aaattggcag ttatgcttgc attgggaatg
     8521 attagctttg gtgcaatggc agttgatggg tataaagatg caaagtttgg catgacagaa
     8581 gaagagtttc tttcgaagag gttatgtgat tttgaaaaat ttgagggaga ttctcgaata
     8641 gaagaagtat cactttattc atgttctgac ttttcgtttg ctaacaaaaa gcgtgaagca
     8701 atggcatttt ttttaaatgg gaaatttaaa agattagaga ttaatattgg cagacttgtg
     8761 aagccagtaa gcaaatcgtt aacgaaaaag tacggagatg gatcatcgta tccatcaaaa
     8821 gaagaatttg agaacgcgct aaaatacaat ggaactatgt ctataggtta tgataataat
     8881 acggtattag ttgatataca tataatatgt ggcaaagaag gcatagaaac cagtcaactg
     8941 atttatacga gtccagatgt ttatacgctc ccagatttcg gagaaaaaat ccaggaatta
     9001 aagggattaa aggaattggg tacctcgcga aggccttgca ggccaaccag ataagtgaaa
     9061 tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca
     9121 gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct
     9181 cccagttccc aactattttg tccgcccaca gcggggcatt tttcttcctg ttatgtttgg
     9241 gcgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     9301 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     9361 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     9421 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     9481 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9541 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9601 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9661 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9721 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9781 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9841 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9901 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9961 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    10021 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    10081 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    10141 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    10201 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    10261 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    10321 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10381 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    10441 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    10501 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    10561 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    10621 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    10681 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10741 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    10801 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    10861 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    10921 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    10981 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    11041 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    11101 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    11161 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    11221 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    11281 atcacgaggc cctttcgtc
//