| 质粒名称: | pY026 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 84741 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4963 bp |
| 完整质粒大小: | 9767 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | huAsCpf1 |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: |
NLS（插入件上的C端子）
3xHA（插入件上的C端子）
|
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | cgcaaatgggcggtaggcgtg |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pY026 was a gift from Feng Zhang (Addgene plasmid # 84741 ; http://n2t.net/addgene:84741 ; RRID:Addgene_84741) |
| 参考文献部分: | Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array. Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F. Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5. 10.1038/nbt.3737 PubMed 27918548 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pY026 9770 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses huAsCpf1 and crRNA guide.
ACCESSION .
VERSION .
KEYWORDS pY026
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9770)
AUTHORS Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J,
DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS,
Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
TITLE Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
JOURNAL Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub
2016 Dec 5.
PUBMED 27918548
REFERENCE 2 (bases 1 to 9770)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9770
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..241
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 1..21
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 172..191
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
enhancer 369..748
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 749..952
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 903..923
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 997..1016
/label=T7
/note="T7 promoter, forward primer"
promoter 997..1015
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1063..4983
/codon_start=1
/product="CRISPR-associated protein from Acidaminococcus
sp. BV3L6 (Zetsche et al., 2015)"
/label=AsCpf1
/translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
KDLKLQNGISNQDWLAYIQELRN"
regulatory 4703..4712
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4984..5031
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 5038..5118
/codon_start=1
/product="three tandem HA epitope tags"
/label=3xHA
/translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
primer_bind complement(5192..5209)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5198..5422
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5468..5896
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5555..5574)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5765..5786
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5905..5925)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5910..6239
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6090..6225
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6152..6171
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6306..7100
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6360..6379)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 6970..6989
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 7274..7395
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7311..7330)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7365..7384
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7444..7460)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7444..7460)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7457..7479)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7468..7484
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7492..7522)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7537..7558
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7675..7692)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7846..8434)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7926..7945)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8605..9465)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9228..9247
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9466..9570)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9645..9664)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
241 cgaaacaccg taatttctac tcttgtagat ggagacgata tatcgtctct ttttcaattg
301 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
361 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
421 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
481 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
541 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
601 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
661 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
721 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
781 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
841 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
901 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct
961 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
1021 ccaagctggc tagcgtttaa acttaagctt ggtaccgcca ccatgacaca gttcgagggc
1081 tttaccaacc tgtatcaggt gagcaagaca ctgcggtttg agctgatccc acagggcaag
1141 accctgaagc acatccagga gcagggcttc atcgaggagg acaaggcccg caatgatcac
1201 tacaaggagc tgaagcccat catcgatcgg atctacaaga cctatgccga ccagtgcctg
1261 cagctggtgc agctggattg ggagaacctg agcgccgcca tcgactccta tagaaaggag
1321 aaaaccgagg agacaaggaa cgccctgatc gaggagcagg ccacatatcg caatgccatc
1381 cacgactact tcatcggccg gacagacaac ctgaccgatg ccatcaataa gagacacgcc
1441 gagatctaca agggcctgtt caaggccgag ctgtttaatg gcaaggtgct gaagcagctg
1501 ggcaccgtga ccacaaccga gcacgagaac gccctgctgc ggagcttcga caagtttaca
1561 acctacttct ccggctttta tgagaacagg aagaacgtgt tcagcgccga ggatatcagc
1621 acagccatcc cacaccgcat cgtgcaggac aacttcccca agtttaagga gaattgtcac
1681 atcttcacac gcctgatcac cgccgtgccc agcctgcggg agcactttga gaacgtgaag
1741 aaggccatcg gcatcttcgt gagcacctcc atcgaggagg tgttttcctt ccctttttat
1801 aaccagctgc tgacacagac ccagatcgac ctgtataacc agctgctggg aggaatctct
1861 cgggaggcag gcaccgagaa gatcaagggc ctgaacgagg tgctgaatct ggccatccag
1921 aagaatgatg agacagccca catcatcgcc tccctgccac acagattcat ccccctgttt
1981 aagcagatcc tgtccgatag gaacaccctg tctttcatcc tggaggagtt taagagcgac
2041 gaggaagtga tccagtcctt ctgcaagtac aagacactgc tgagaaacga gaacgtgctg
2101 gagacagccg aggccctgtt taacgagctg aacagcatcg acctgacaca catcttcatc
2161 agccacaaga agctggagac aatcagcagc gccctgtgcg accactggga tacactgagg
2221 aatgccctgt atgagcggag aatctccgag ctgacaggca agatcaccaa gtctgccaag
2281 gagaaggtgc agcgcagcct gaagcacgag gatatcaacc tgcaggagat catctctgcc
2341 gcaggcaagg agctgagcga ggccttcaag cagaaaacca gcgagatcct gtcccacgca
2401 cacgccgccc tggatcagcc actgcctaca accctgaaga agcaggagga gaaggagatc
2461 ctgaagtctc agctggacag cctgctgggc ctgtaccacc tgctggactg gtttgccgtg
2521 gatgagtcca acgaggtgga ccccgagttc tctgcccggc tgaccggcat caagctggag
2581 atggagcctt ctctgagctt ctacaacaag gccagaaatt atgccaccaa gaagccctac
2641 tccgtggaga agttcaagct gaactttcag atgcctacac tggcctctgg ctgggacgtg
2701 aataaggaga agaacaatgg cgccatcctg tttgtgaaga acggcctgta ctatctgggc
2761 atcatgccaa agcagaaggg caggtataag gccctgagct tcgagcccac agagaaaacc
2821 agcgagggct ttgataagat gtactatgac tacttccctg atgccgccaa gatgatccca
2881 aagtgcagca cccagctgaa ggccgtgaca gcccactttc agacccacac aacccccatc
2941 ctgctgtcca acaatttcat cgagcctctg gagatcacaa aggagatcta cgacctgaac
3001 aatcctgaga aggagccaaa gaagtttcag acagcctacg ccaagaaaac cggcgaccag
3061 aagggctaca gagaggccct gtgcaagtgg atcgacttca caagggattt tctgtccaag
3121 tataccaaga caacctctat cgatctgtct agcctgcggc catcctctca gtataaggac
3181 ctgggcgagt actatgccga gctgaatccc ctgctgtacc acatcagctt ccagagaatc
3241 gccgagaagg agatcatgga tgccgtggag acaggcaagc tgtacctgtt ccagatctat
3301 aacaaggact ttgccaaggg ccaccacggc aagcctaatc tgcacacact gtattggacc
3361 ggcctgtttt ctccagagaa cctggccaag acaagcatca agctgaatgg ccaggccgag
3421 ctgttctacc gccctaagtc caggatgaag aggatggcac accggctggg agagaagatg
3481 ctgaacaaga agctgaagga tcagaaaacc ccaatccccg acaccctgta ccaggagctg
3541 tacgactatg tgaatcacag actgtcccac gacctgtctg atgaggccag ggccctgctg
3601 cccaacgtga tcaccaagga ggtgtctcac gagatcatca aggataggcg ctttaccagc
3661 gacaagttct ttttccacgt gcctatcaca ctgaactatc aggccgccaa ttccccatct
3721 aagttcaacc agagggtgaa tgcctacctg aaggagcacc ccgagacacc tatcatcggc
3781 atcgatcggg gcgagagaaa cctgatctat atcacagtga tcgactccac cggcaagatc
3841 ctggagcagc ggagcctgaa caccatccag cagtttgatt accagaagaa gctggacaac
3901 agggagaagg agagggtggc agcaaggcag gcctggtctg tggtgggcac aatcaaggat
3961 ctgaagcagg gctatctgag ccaggtcatc cacgagatcg tggacctgat gatccactac
4021 caggccgtgg tggtgctgga gaacctgaat ttcggcttta agagcaagag gaccggcatc
4081 gccgagaagg ccgtgtacca gcagttcgag aagatgctga tcgataagct gaattgcctg
4141 gtgctgaagg actatccagc agagaaagtg ggaggcgtgc tgaacccata ccagctgaca
4201 gaccagttca cctcctttgc caagatgggc acccagtctg gcttcctgtt ttacgtgcct
4261 gccccatata catctaagat cgatcccctg accggcttcg tggacccctt cgtgtggaaa
4321 accatcaaga atcacgagag ccgcaagcac ttcctggagg gcttcgactt tctgcactac
4381 gacgtgaaaa ccggcgactt catcctgcac tttaagatga acagaaatct gtccttccag
4441 aggggcctgc ccggctttat gcctgcatgg gatatcgtgt tcgagaagaa cgagacacag
4501 tttgacgcca agggcacccc tttcatcgcc ggcaagagaa tcgtgccagt gatcgagaat
4561 cacagattca ccggcagata ccgggacctg tatcctgcca acgagctgat cgccctgctg
4621 gaggagaagg gcatcgtgtt cagggatggc tccaacatcc tgccaaagct gctggagaat
4681 gacgattctc acgccatcga caccatggtg gccctgatcc gcagcgtgct gcagatgcgg
4741 aactccaatg ccgccacagg cgaggactat atcaacagcc ccgtgcgcga tctgaatggc
4801 gtgtgcttcg actcccggtt tcagaaccca gagtggccca tggacgccga tgccaatggc
4861 gcctaccaca tcgccctgaa gggccagctg ctgctgaatc acctgaagga gagcaaggat
4921 ctgaagctgc agaacggcat ctccaatcag gactggctgg cctacatcca ggagctgcgc
4981 aacaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa gggatcctac
5041 ccatacgatg ttccagatta cgcttatccc tacgacgtgc ctgattatgc atacccatat
5101 gatgtccccg actatgccta agaattctgc agatatccag cacagtggcg gccgctcgag
5161 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
5221 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
5281 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
5341 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
5401 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
5461 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
5521 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
5581 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
5641 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
5701 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
5761 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
5821 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
5881 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
5941 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
6001 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
6061 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
6121 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
6181 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
6241 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
6301 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
6361 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
6421 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
6481 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
6541 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
6601 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
6661 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
6721 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
6781 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
6841 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
6901 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
6961 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
7021 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
7081 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
7141 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
7201 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
7261 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
7321 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
7381 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
7441 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
7501 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
7561 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
7621 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
7681 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
7741 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
7801 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
7861 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
7921 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
7981 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
8041 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
8101 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
8161 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
8221 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
8281 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
8341 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
8401 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
8461 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
8521 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
8581 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
8641 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
8701 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
8761 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
8821 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
8881 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
8941 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
9001 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
9061 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
9121 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
9181 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
9241 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
9301 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
9361 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
9421 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
9481 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
9541 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
9601 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
9661 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
9721 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acttaattaa
//
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