| 质粒名称: | pGD-P1/HC-Pro<质粒图谱.png/td> |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 196328 |
| 存储实验室: | Zhenghe Li |
|
载体骨架基本信息 |
|
| 载体名称: | pGD |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 9855 bp |
| 载体类型: | 植物表达 |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | tobacco etch virus P1/HC-Pro / TEV P1/HC-Pro |
| 物种来源: | tobacco etch virus |
| 启动子: | duplicated cauliflower mosaic virus 35S promoter |
| 克隆方法: | Ligation Independent Cloning |
| 5’测序引物 | 35S promoter/forward primer: CTATCCTTCGCAAGACCCTTC |
| 3’测序引物 | Nos terminator/reverse primer: TCATCGCAAGACCGGCAA |
|
文献引用方法 |
|
| 材料和方法部分: |
pGD-P1/HC-Pro was a gift from Zhenghe Li (Addgene plasmid # 196328 ; http://n2t.net/addgene:196328 ; RRID:Addgene_196328)
|
| 参考文献部分: | Construction of a Sonchus Yellow Net Virus minireplicon: a step toward reverse genetic analysis of plant negative-strand RNA viruses. Ganesan U, Bragg JN, Deng M, Marr S, Lee MY, Qian S, Shi M, Kappel J, Peters C, Lee Y, Goodin MM, Dietzgen RG, Li Z, Jackson AO. J Virol. 2013 Oct;87(19):10598-611. doi: 10.1128/JVI.01397-13. Epub 2013 Jul 24. 10.1128/JVI.01397-13 PubMed 23885070 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGD-P1/HC-Pro 9855 bp ds-DNA circular SYN 12-APR-2023
DEFINITION 35S promoter-driven expression of the tobacco etch virus P1/HC-Pro
RNA silencing suppressor.
ACCESSION .
VERSION .
KEYWORDS pGD-P1/HC-Pro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9855)
AUTHORS Ganesan U, Bragg JN, Deng M, Marr S, Lee MY, Qian S, Shi M, Kappel
J, Peters C, Lee Y, Goodin MM, Dietzgen RG, Li Z, Jackson AO
TITLE Construction of a Sonchus Yellow Net Virus minireplicon: a step
toward reverse genetic analysis of plant negative-strand RNA
viruses.
JOURNAL J Virol. 2013 Oct;87(19):10598-611. doi: 10.1128/JVI.01397-13. Epub
2013 Jul 24.
PUBMED 23885070
REFERENCE 2 (bases 1 to 9855)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 12, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9855
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 5..29
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
promoter 179..524
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind 467..487
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
terminator 3306..3558
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 3580..3604
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 4905..5534
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 5963..7036
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 7102..7296
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(7532..7554)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 7640..7780
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 7695..7714
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(7795..7812)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7966..8554)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8046..8065)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8641..9435)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
1 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
61 tgcggacgtt tttaatgtac tgaattaacg ccgaattaat acgcgtcatg gtggagcacg
121 acactcttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg
181 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct
241 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
301 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc
361 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
421 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
481 acccttcctc tatataagga agttcatttc atttggagag gacacgctga aatcaccagt
541 ctctctctac aaatctatct ctggatcccc agtctctctc tacaagatct cgagaattct
601 caacacaaca tatacaaaac aaacgaatct caagcaatca agcattctac ttctattgca
661 gcaatttaaa tcatttcttt taaagcaaaa gcaattttct gaaaattttc accatttacg
721 aacgatagcc atggcactca tctttggcac agtcaacgct aacatcctga aggaagtgtt
781 cggtggagct cgtatggctt gcgttaccag cgcacatatg gctggagcga atggaagcat
841 tttgaagaag gcagaagaga cctctcgtgc aatcatgcac aaaccagtga tcttcggaga
901 agactacatt accgaggcag acttgcctta cacaccactc catttagagg tcgatgctga
961 aatggagcgg atgtattatc ttggtcgtcg cgcgctcacc catggcaaga gacgcaaagt
1021 ttctgtgaat aacaagagga acaggagaag gaaagtggcc aaaacgtacg tggggcgtga
1081 ttccattgtt gagaagattg tagtgcccca caccgagaga aaggttgata ccacagcagc
1141 agtggaagac atttgcaatg aagctaccac tcaacttgtg cataatagta tgccaaagcg
1201 taagaagcag aaaaacttct tgcccgccac ttcactaagt aacgtgtatg cccaaacttg
1261 gagcatagtg cgcaaacgcc atatgcaggt ggagatcatt agcaagaaga gcgtccgagc
1321 gagggtcaag agatttgagg gctcggtgca attgttcgca agtgtgcgtc acatgtatgg
1381 cgagaggaaa agggtggact tacgtattga caactggcag caagagacac ttctagacct
1441 tgctaaaaga tttaagaatg agagagtgga tcaatcgaag ctcacttttg gttcaagtgg
1501 cctagttttg aggcaaggct cgtacggacc tgcgcattgg tatcgacatg gtatgttcat
1561 tgtacgcggt cggtcggatg ggatgttggt ggatgctcgt gcgaaggtaa cgttcgctgt
1621 ttgtcactca atgacacatt atagcgacaa atcaatctct gaggcattct tcataccata
1681 ctctaagaaa ttcttggagt tgagaccaga tggaatctcc catgagtgta caagaggagt
1741 atcagttgag cggtgcggtg aggtggctgc aatcctgaca caagcacttt caccgtgtgg
1801 taagatcaca tgcaaacgtt gcatggttga aacacctgac attgttgagg gtgagtcggg
1861 agacagtgtc accaaccaag gtaagctcct agcaatgctg aaagaacagt atccagattt
1921 cccaatggcc gagaaactac tcacaaggtt tttgcaacag aaatcactag taaatacaaa
1981 tttgacagcc tgcgtgagcg tcaaacaact cattggtgac cgcaaacaag ctccattcac
2041 acacgtactg gctgtcagcg aaattctgtt taaaggcaat aaactaacag gggctgatct
2101 cgaagaggca agcacacata tgcttgaaat agcaaggttc ttgaacaatc gcactgaaaa
2161 tatgcgcatt ggccaccttg gttctttcag aaataaaatc tcatcgaagg cccatgtgaa
2221 taacgcactc atgtgtgata atcaacttga tcagaatggg aattttattt ggggactaag
2281 gggtgcacac gcaaagaggt ttcttaaagg atttttcact gagattgacc caaatgaagg
2341 atacgataag tatgttatca ggaaacatat caggggtagc agaaagctag caattggcaa
2401 tttgataatg tcaactgact tccagacgct caggcaacaa attcaaggcg aaactattga
2461 gcgtaaagaa attgggaatc actgcatttc aatgcggaat ggtaattacg tgtacccatg
2521 ttgttgtgtt actcttgaag atggtaaggc tcaatattcg gatctaaagc acccaacgaa
2581 gagacatctg gtcattggca actctggcga ttcaaagtac ctagaccttc cagttctcaa
2641 tgaagagaaa atgtatatag ctaatgaagg ttattgctac atgaacattt tctttgctct
2701 actagtgaat gtcaaggaag aggatgcaaa ggacttcacc aagtttataa gggacacaat
2761 tgttccaaag cttggagcgt ggccaacaat gcaagatgtt gcaactgcat gctacttact
2821 ttccattctt tacccagatg tcctgagtgc tgaactaccc agaattttgg ttgatcatga
2881 caacaaaaca atgcatgttt tggattcgta tgggtctaga acgacaggat accacatgtt
2941 gaaaatgaac acaacatccc agctaattga attcgttcat tcaggtttgg aatccgaaat
3001 gaaaacttac aatgttggag ggatgaaccg agatatggtc acacaaggtg caattgagat
3061 gttgatcaag tctatataca aaccacatct catgaagcag ttacttgagg aggagccata
3121 cataattgtc ctggcaatag tctccccttc aattttaatt gccatgtaca actctggaac
3181 ttttgagcag gcgttacaaa tgtggttgcc aaatacaatg aggttagcta acctcgctgc
3241 catcttgtca gccttagcgc aaaagttggg gatccaccgg atctagataa ctgatcgtcg
3301 acagagctcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
3361 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
3421 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
3481 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
3541 catctatgtt actagatcgg gaattaaact atcagtgttt gacaggatat attggcgggt
3601 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
3661 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag
3721 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
3781 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
3841 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
3901 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
3961 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
4021 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
4081 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
4141 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
4201 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
4261 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
4321 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
4381 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
4441 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
4501 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
4561 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
4621 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
4681 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt
4741 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
4801 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
4861 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
4921 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
4981 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
5041 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
5101 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
5161 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
5221 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
5281 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
5341 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
5401 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
5461 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
5521 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
5581 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
5641 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
5701 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
5761 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
5821 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
5881 cgggcggttg gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa
5941 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
6001 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
6061 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
6121 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
6181 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
6241 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
6301 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
6361 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
6421 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
6481 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
6541 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
6601 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
6661 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
6721 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
6781 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
6841 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
6901 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
6961 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
7021 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
7081 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
7141 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
7201 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
7261 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
7321 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
7381 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
7441 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
7501 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
7561 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
7621 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
7681 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
7741 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
7801 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
7861 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
7921 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
7981 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
8041 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
8101 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
8161 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
8221 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
8281 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
8341 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
8401 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
8461 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
8521 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
8581 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta
8641 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
8701 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
8761 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
8821 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
8881 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
8941 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
9001 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
9061 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
9121 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
9181 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
9241 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
9301 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
9361 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
9421 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
9481 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
9541 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
9601 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
9661 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
9721 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
9781 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
9841 ggagctgttg gctgg
//
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