质粒名称: | pUC-AdEvolve-DEST |
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出品公司: | Addgene |
目录编号: | 131692 |
存储实验室: | Matthew D. Shoulders |
载体骨架基本信息 |
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载体名称: | pAd⁄CMV⁄V5-DEST |
载体出品公司: | Thermo Fisher Scientific |
空载体大小: | 36831 bp |
完整质粒大小: | 36836 bp |
载体类型: | Adenoviral |
载体抗性: | 氨苄霉素 |
生长温度: | 37 ℃ |
宿主菌: | DH10B gyrA462 |
拷贝数: | 高拷贝 |
插入基因信息 |
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克隆方法: | Gateway Cloning |
文献引用方法 |
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材料和方法部分: | pUC-AdEvolve-DEST was a gift from Matthew D. Shoulders (Addgene plasmid # 131692 ; http://n2t.net/addgene:131692 ; RRID:Addgene_131692) |
参考文献部分: | An Adaptable Platform for Directed Evolution in Human Cells. Berman CM, Papa LJ 3rd, Hendel SJ, Moore CL, Suen PH, Weickhardt AF, Doan ND, Kumar CM, Uil TG, Butty VL, Hoeben RC, Shoulders MD. J Am Chem Soc. 2018 Dec 26;140(51):18093-18103. doi: 10.1021/jacs.8b10937. Epub 2018 Dec 14. 10.1021/jacs.8b10937 PubMed 30427676 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pUC-AdEvolve-DEST 36836 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION A version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.. ACCESSION . VERSION . KEYWORDS pUC-AdEvolve-DEST SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 36836) AUTHORS Berman CM, Papa LJ 3rd, Hendel SJ, Moore CL, Suen PH, Weickhardt AF, Doan ND, Kumar CM, Uil TG, Butty VL, Hoeben RC, Shoulders MD TITLE An Adaptable Platform for Directed Evolution in Human Cells. JOURNAL J Am Chem Soc. 2018 Dec 26;140(51):18093-18103. doi: 10.1021/jacs.8b10937. Epub 2018 Dec 14. PUBMED 30427676 REFERENCE 2 (bases 1 to 36836) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..36836 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..103 /label=ITR /note="inverted terminal repeat of human adenovirus serotype 5" misc_signal 191..341 /label=Ad5 Psi /note="packaging signal for adenovirus serotype 5" polyA_signal 347..571 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind 560..577 /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" CDS complement(600..617) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS complement(627..668) /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" protein_bind 694..818 /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS complement(859..1164) /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind complement(961..980) /label=ccdB-fwd /note="ccdB gene, forward primer" CDS complement(1506..2165) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 2080..2099 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(2219..2249) /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS complement(2312..3115) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2423..2442) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 3033..3052 /label=Neo-R /note="Neomycin resistance gene, reverse primer" promoter complement(3134..3181) /label=EM7 promoter /note="synthetic bacterial promoter " protein_bind complement(3206..3330) /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(3359..3378) /label=T7 /note="T7 promoter, forward primer" promoter complement(3360..3378) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter complement(3423..3626) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind complement(3452..3472) /label=CMV-F /note="Human CMV immediate early promoter, forward primer" enhancer 3627..4006 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" misc_feature 7542..7645 /label=Ad SA /note="adenovirus major late transcript splice acceptor (Friedrich and Soriano, 1991)" CDS complement(14168..14179) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(33205..33225) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 33210..33539 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 33390..33525 /label=SV40 ori /note="SV40 origin of replication" primer_bind 33452..33471 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 33560..34270 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(33705..33723) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 33973..33992 /label=mCherry-F /note="mCherry, forward primer" polyA_signal 34430..34551 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(34467..34486) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 34521..34540 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" repeat_region 34647..34749 /label=ITR /note="inverted terminal repeat of human adenovirus serotype 5" rep_origin complement(34973..35561) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(35053..35072) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(35713..36573) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 36336..36355 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(36574..36678) /gene="bla" /label=AmpR promoter primer_bind 36746..36764 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tagaagccat agagcccacc 361 gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc 421 ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat 481 taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa 541 acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgggt ttaaactcaa 601 tggtgatggt gatgatgacc ggtacgcgta gaatcgagac cgaggagagg gttagggata 661 ggcttacctt cgaaccgcgg gccctctaga tcaaccactt tgtacaagaa agctgaacga 721 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact 781 acataatact gtaaaacaca acatatccag tcactatggt cgacctgcag actggctgtg 841 tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc 901 attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc 961 catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc 1021 acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc 1081 gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt 1141 ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca aaagagccgt 1201 tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt 1261 cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga 1321 catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg 1381 cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata tattcttata 1441 ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gccggatcca 1501 gatctttacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg 1561 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc 1621 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata 1681 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac 1741 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct 1801 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa 1861 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc 1921 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga 1981 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc 2041 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca 2101 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc 2161 tccattttag cttccttagc tcctgaaaat ctcgacggat cctaactcaa aatccacaca 2221 ttatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgcggc cgcgtcgagg 2281 ctgatcagcg agctctagag aattgatccc ctcagaagaa ctcgtcaaga aggcgataga 2341 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc 2401 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt 2461 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga 2521 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc atcgccgtcg ggcatgcgcg 2581 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat 2641 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt 2701 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca 2761 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt 2821 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag 2881 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg 2941 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca 3001 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca 3061 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatggccgat cccatggttt 3121 agttcctcac cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa 3181 caggctgcag gtcgaaagcg gccgccatag tgactggata tgttgtgttt tacagtatta 3241 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt 3301 ttctcgttca gcttttttgt acaaacttgt tgatagctta actagccagc ttgggtctcc 3361 ctatagtgag tcgtattaat ttcgataagc cagtaagcag tgggttctct agttagccag 3421 agagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg 3481 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca 3541 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca 3601 ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac 3661 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta 3721 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag 3781 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt 3841 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca 3901 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat 3961 gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcaacg cgtatatctg 4021 tgggcgtggc ttaagggtgg gaaagaatat ataaggtggg ggtcttatgt agttttgtat 4081 ctgttttgca gcagccgccg ccgccatgag caccaactcg tttgatggaa gcattgtgag 4141 ctcatatttg acaacgcgca tgcccccatg ggccggggtg cgtcagaatg tgatgggctc 4201 cagcattgat ggtcgccccg tcctgcccgc aaactctact accttgacct acgagaccgt 4261 gtctggaacg ccgttggaga ctgcagcctc cgccgccgct tcagccgctg cagccaccgc 4321 ccgcgggatt gtgactgact ttgctttcct gagcccgctt gcaagcagtg cagcttcccg 4381 ttcatccgcc cgcgatgaca agttgacggc tcttttggca caattggatt ctttgacccg 4441 ggaacttaat gtcgtttctc agcagctgtt ggatctgcgc cagcaggttt ctgccctgaa 4501 ggcttcctcc cctcccaatg cggtttaaaa cataaataaa aaaccagact ctgtttggat 4561 ttggatcaag caagtgtctt gctgtcttta tttaggggtt ttgcgcgcgc ggtaggcccg 4621 ggaccagcgg tctcggtcgt tgagggtcct gtgtattttt tccaggacgt ggtaaaggtg 4681 actctggatg ttcagataca tgggcataag cccgtctctg gggtggaggt agcaccactg 4741 cagagcttca tgctgcgggg tggtgttgta gatgatccag tcgtagcagg agcgctgggc 4801 gtggtgccta aaaatgtctt tcagtagcaa gctgattgcc aggggcaggc ccttggtgta 4861 agtgtttaca aagcggttaa gctgggatgg gtgcatacgt ggggatatga gatgcatctt 4921 ggactgtatt tttaggttgg ctatgttccc agccatatcc ctccggggat tcatgttgtg 4981 cagaaccacc agcacagtgt atccggtgca cttgggaaat ttgtcatgta gcttagaagg 5041 aaatgcgtgg aagaacttgg agacgccctt gtgacctcca agattttcca tgcattcgtc 5101 cataatgatg gcaatgggcc cacgggcggc ggcctgggcg aagatatttc tgggatcact 5161 aacgtcatag ttgtgttcca ggatgagatc gtcataggcc atttttacaa agcgcgggcg 5221 gagggtgcca gactgcggta taatggttcc atccggccca ggggcgtagt taccctcaca 5281 gatttgcatt tcccacgctt tgagttcaga tggggggatc atgtctacct gcggggcgat 5341 gaagaaaacg gtttccgggg taggggagat cagctgggaa gaaagcaggt tcctgagcag 5401 ctgcgactta ccgcagccgg tgggcccgta aatcacacct attaccggct gcaactggta 5461 gttaagagag ctgcagctgc cgtcatccct gagcaggggg gccacttcgt taagcatgtc 5521 cctgactcgc atgttttccc tgaccaaatc cgccagaagg cgctcgccgc ccagcgatag 5581 cagttcttgc aaggaagcaa agtttttcaa cggtttgaga ccgtccgccg taggcatgct 5641 tttgagcgtt tgaccaagca gttccaggcg gtcccacagc tcggtcacct gctctacggc 5701 atctcgatcc agcatatctc ctcgtttcgc gggttggggc ggctttcgct gtacggcagt 5761 agtcggtgct cgtccagacg ggccagggtc atgtctttcc acgggcgcag ggtcctcgtc 5821 agcgtagtct gggtcacggt gaaggggtgc gctccgggct gcgcgctggc cagggtgcgc 5881 ttgaggctgg tcctgctggt gctgaagcgc tgccggtctt cgccctgcgc gtcggccagg 5941 tagcatttga ccatggtgtc atagtccagc ccctccgcgg cgtggccctt ggcgcgcagc 6001 ttgcccttgg aggaggcgcc gcacgagggg cagtgcagac ttttgagggc gtagagcttg 6061 ggcgcgagaa ataccgattc cggggagtag gcatccgcgc cgcaggcccc gcagacggtc 6121 tcgcattcca cgagccaggt gagctctggc cgttcggggt caaaaaccag gtttccccca 6181 tgctttttga tgcgtttctt acctctggtt tccatgagcc ggtgtccacg ctcggtgacg 6241 aaaaggctgt ccgtgtcccc gtatacagac ttgagaggcc tgtcctcgag cggtgttccg 6301 cggtcctcct cgtatagaaa ctcggaccac tctgagacaa aggctcgcgt ccaggccagc 6361 acgaaggagg ctaagtggga ggggtagcgg tcgttgtcca ctagggggtc cactcgctcc 6421 agggtgtgaa gacacatgtc gccctcttcg gcatcaagga aggtgattgg tttgtaggtg 6481 taggccacgt gaccgggtgt tcctgaaggg gggctataaa agggggtggg ggcgcgttcg 6541 tcctcactct cttccgcatc gctgtctgcg agggccagct gttggggtga gtactccctc 6601 tgaaaagcgg gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg 6661 atattcacct ggcccgcggt gatgcctttg agggtggccg catccatctg gtcagaaaag 6721 acaatctttt tgttgtcaag cttggtggca aacgacccgt agagggcgtt ggacagcaac 6781 ttggcgatgg agcgcagggt ttggtttttg tcgcgatcgg cgcgctcctt ggccgcgatg 6841 tttagctgca cgtattcgcg cgcaacgcac cgccattcgg gaaagacggt ggtgcgctcg 6901 tcgggcacca ggtgcacgcg ccaaccgcgg ttgtgcaggg tgacaaggtc aacgctggtg 6961 gctacctctc cgcgtaggcg ctcgttggtc cagcagaggc ggccgccctt gcgcgagcag 7021 aatggcggta gggggtctag ctgcgtctcg tccggggggt ctgcgtccac ggtaaagacc 7081 ccgggcagca ggcgcgcgtc gaagtagtct atcttgcatc cttgcaagtc tagcgcctgc 7141 tgccatgcgc gggcggcaag cgcgcgctcg tatgggttga gtgggggacc ccatggcatg 7201 gggtgggtga gcgcggaggc gtacatgccg caaatgtcgt aaacgtagag gggctctctg 7261 agtattccaa gatatgtagg gtagcatctt ccaccgcgga tgctggcgcg cacgtaatcg 7321 tatagttcgt gcgagggagc gaggaggtcg ggaccgaggt tgctacgggc gggctgctct 7381 gctcggaaga ctatctgcct gaagatggca tgtgagttgg atgatatggt tggacgctgg 7441 aagacgttga agctggcgtc tgtgagacct accgcgtcac gcacgaagga ggcgtaggag 7501 tcgcgcagct tgttgaccag ctcggcggtg acctgcacgt ctagggcgca gtagtccagg 7561 gtttccttga tgatgtcata cttatcctgt cccttttttt tccacagctc gcggttgagg 7621 acaaactctt cgcggtcttt ccagtactct tggatcggaa acccgtcggc ctccgaacgg 7681 taagagccta gcatgtagaa ctggttgacg gcctggtagg cgcagcatcc cttttctacg 7741 ggtagcgcgt atgcctgcgc ggccttccgg agcgaggtgt gggtgagcgc aaaggtgtcc 7801 ctgaccatga ccagcatgaa gggcacgagc tgcttcccaa aggcccccat ccaagtatag 7861 gtctctacat cgtaggtgac aaagagacgc tcggtgcgag gatgcgagcc gatcgggaag 7921 aactggatct cccgccacca attggaggag tggctattga tgtggtgaaa gtagaagtcc 7981 ctgcgacggg ccgaacactc gtgctggctt ttgtaaaaac gtgcgcagta ctggcagcgg 8041 tgcacgggct gtacatcctg cacgaggttg acctgacgac cgcgcacaag gaagcagagt 8101 gggaatttga gcccctcgcc tggcgggttt ggctggtggt cttctacttc ggctgcttgt 8161 ccttgaccgt ctggctgctc gaggggagtt acggtggatc ggaccaccac gccgcgcgag 8221 cccaaagtcc agatgtccgc gcgcggcggt cggagcttga tgacaacatc gcgcagatgg 8281 gagctgtcca tggtctggag ctcccgcggc gtcaggtcag gcgggagctc ctgcaggttt 8341 acctcgcata gacgggtcag ggcgcgggct agatccaggt gatacctaat ttccaggggc 8401 tggttggtgg cggcgtcgat ggcttgcaag aggccgcatc cccgcggcgc gactacggta 8461 ccgcgcggcg ggcggtgggc cgcgggggtg tccttggatg atgcatctaa aagcggtgac 8521 gcgggcgagc ccccggaggt agggggggct ccggacccgc cgggagaggg ggcaggggca 8581 cgtcggcgcc gcgcgcgggc aggagctggt gctgcgcgcg taggttgctg gcgaacgcga 8641 cgacgcggcg gttgatctcc tgaatctggc gcctctgcgt gaagacgacg ggcccggtga 8701 gcttgaacct gaaagagagt tcgacagaat caatttcggt gtcgttgacg gcggcctggc 8761 gcaaaatctc ctgcacgtct cctgagttgt cttgataggc gatctcggcc atgaactgct 8821 cgatctcttc ctcctggaga tctccgcgtc cggctcgctc cacggtggcg gcgaggtcgt 8881 tggaaatgcg ggccatgagc tgcgagaagg cgttgaggcc tccctcgttc cagacgcggc 8941 tgtagaccac gcccccttcg gcatcgcggg cgcgcatgac cacctgcgcg agattgagct 9001 ccacgtgccg ggcgaagacg gcgtagtttc gcaggcgctg aaagaggtag ttgagggtgg 9061 tggcggtgtg ttctgccacg aagaagtaca taacccagcg tcgcaacgtg gattcgttga 9121 tatcccccaa ggcctcaagg cgctccatgg cctcgtagaa gtccacggcg aagttgaaaa 9181 actgggagtt gcgcgccgac acggttaact cctcctccag aagacggatg agctcggcga 9241 cagtgtcgcg cacctcgcgc tcaaaggcta caggggcctc ttcttcttct tcaatctcct 9301 cttccataag ggcctcccct tcttcttctt ctggcggcgg tgggggaggg gggacacggc 9361 ggcgacgacg gcgcaccggg aggcggtcga caaagcgctc gatcatctcc ccgcggcgac 9421 ggcgcatggt ctcggtgacg gcgcggccgt tctcgcgggg gcgcagttgg aagacgccgc 9481 ccgtcatgtc ccggttatgg gttggcgggg ggctgccatg cggcagggat acggcgctaa 9541 cgatgcatct caacaattgt tgtgtaggta ctccgccgcc gagggacctg agcgagtccg 9601 catcgaccgg atcggaaaac ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta 9661 ggctgagcac cgtggcgggc ggcagcgggc ggcggtcggg gttgtttctg gcggaggtgc 9721 tgctgatgat gtaattaaag taggcggtct tgagacggcg gatggtcgac agaagcacca 9781 tgtccttggg tccggcctgc tgaatgcgca ggcggtcggc catgccccag gcttcgtttt 9841 gacatcggcg caggtctttg tagtagtctt gcatgagcct ttctaccggc acttcttctt 9901 ctccttcctc ttgtcctgca tctcttgcat ctatcgctgc ggcggcggcg gagtttggcc 9961 gtaggtggcg ccctcttcct cccatgcgtg tgaccccgaa gcccctcatc ggctgaagca 10021 gggctaggtc ggcgacaacg cgctcggcta atatggcctg ctgcacctgc gtgagggtag 10081 actggaagtc atccatgtcc acaaagcggt ggtatgcgcc cgtgttgatg gtgtaagtgc 10141 agttggccat aacggaccag ttaacggtct ggtgacccgg ctgcgagagc tcggtgtacc 10201 tgagacgcga gtaagccctc gagtcaaata cgtagtcgtt gcaagtccgc accaggtact 10261 ggtatcccac caaaaagtgc ggcggcggct ggcggtagag gggccagcgt agggtggccg 10321 gggctccggg ggcgagatct tccaacataa ggcgatgata tccgtagatg tacctggaca 10381 tccaggtgat gccggcggcg gtggtggagg cgcgcggaaa gtcgcggacg cggttccaga 10441 tgttgcgcag cggcaaaaag tgctccatgg tcgggacgct ctggccggtc aggcgcgcgc 10501 aatcgttgac gctctagacc gtgcaaaagg agagcctgta agcgggcact cttccgtggt 10561 ctggtggata aattcgcaag ggtatcatgg cggacgaccg gggttcgagc cccgtatccg 10621 gccgtccgcc gtgatccatg cggttaccgc ccgcgtgtcg aacccaggtg tgcgacgtca 10681 gacaacgggg gagtgctcct tttggcttcc ttccaggcgc ggcggctgct gcgctagctt 10741 ttttggccac tggccgcgcg cagcgtaagc ggttaggctg gaaagcgaaa gcattaagtg 10801 gctcgctccc tgtagccgga gggttatttt ccaagggttg agtcgcggga cccccggttc 10861 gagtctcgga ccggccggac tgcggcgaac gggggtttgc ctccccgtca tgcaagaccc 10921 cgcttgcaaa ttcctccgga aacagggacg agcccctttt ttgcttttcc cagatgcatc 10981 cggtgctgcg gcagatgcgc ccccctcctc agcagcggca agagcaagag cagcggcaga 11041 catgcagggc accctcccct cctcctaccg cgtcaggagg ggcgacatcc gcggttgacg 11101 cggcagcaga tggtgattac gaacccccgc ggcgccgggc ccggcactac ctggacttgg 11161 aggagggcga gggcctggcg cggctaggag cgccctctcc tgagcggcac ccaagggtgc 11221 agctgaagcg tgatacgcgt gaggcgtacg tgccgcggca gaacctgttt cgcgaccgcg 11281 agggagagga gcccgaggag atgcgggatc gaaagttcca cgcagggcgc gagctgcggc 11341 atggcctgaa tcgcgagcgg ttgctgcgcg aggaggactt tgagcccgac gcgcgaaccg 11401 ggattagtcc cgcgcgcgca cacgtggcgg ccgccgacct ggtaaccgca tacgagcaga 11461 cggtgaacca ggagattaac tttcaaaaaa gctttaacaa ccacgtgcgt acgcttgtgg 11521 cgcgcgagga ggtggctata ggactgatgc atctgtggga ctttgtaagc gcgctggagc 11581 aaaacccaaa tagcaagccg ctcatggcgc agctgttcct tatagtgcag cacagcaggg 11641 acaacgaggc attcagggat gcgctgctaa acatagtaga gcccgagggc cgctggctgc 11701 tcgatttgat aaacatcctg cagagcatag tggtgcagga gcgcagcttg agcctggctg 11761 acaaggtggc cgccatcaac tattccatgc ttagcctggg caagttttac gcccgcaaga 11821 tataccatac cccttacgtt cccatagaca aggaggtaaa gatcgagggg ttctacatgc 11881 gcatggcgct gaaggtgctt accttgagcg acgacctggg cgtttatcgc aacgagcgca 11941 tccacaaggc cgtgagcgtg agccggcggc gcgagctcag cgaccgcgag ctgatgcaca 12001 gcctgcaaag ggccctggct ggcacgggca gcggcgatag agaggccgag tcctactttg 12061 acgcgggcgc tgacctgcgc tgggccccaa gccgacgcgc cctggaggca gctggggccg 12121 gacctgggct ggcggtggca cccgcgcgcg ctggcaacgt cggcggcgtg gaggaatatg 12181 acgaggacga tgagtacgag ccagaggacg gcgagtacta agcggtgatg tttctgatca 12241 gatgatgcaa gacgcaacgg acccggcggt gcgggcggcg ctgcagagcc agccgtccgg 12301 ccttaactcc acggacgact ggcgccaggt catggaccgc atcatgtcgc tgactgcgcg 12361 caatcctgac gcgttccggc agcagccgca ggccaaccgg ctctccgcaa ttctggaagc 12421 ggtggtcccg gcgcgcgcaa accccacgca cgagaaggtg ctggcgatcg taaacgcgct 12481 ggccgaaaac agggccatcc ggcccgacga ggccggcctg gtctacgacg cgctgcttca 12541 gcgcgtggct cgttacaaca gcggcaacgt gcagaccaac ctggaccggc tggtggggga 12601 tgtgcgcgag gccgtggcgc agcgtgagcg cgcgcagcag cagggcaacc tgggctccat 12661 ggttgcacta aacgccttcc tgagtacaca gcccgccaac gtgccgcggg gacaggagga 12721 ctacaccaac tttgtgagcg cactgcggct aatggtgact gagacaccgc aaagtgaggt 12781 gtaccagtct gggccagact attttttcca gaccagtaga caaggcctgc agaccgtaaa 12841 cctgagccag gctttcaaaa acttgcaggg gctgtggggg gtgcgggctc ccacaggcga 12901 ccgcgcgacc gtgtctagct tgctgacgcc caactcgcgc ctgttgctgc tgctaatagc 12961 gcccttcacg gacagtggca gcgtgtcccg ggacacatac ctaggtcact tgctgacact 13021 gtaccgcgag gccataggtc aggcgcatgt ggacgagcat actttccagg agattacaag 13081 tgtcagccgc gcgctggggc aggaggacac gggcagcctg gaggcaaccc taaactacct 13141 gctgaccaac cggcggcaga agatcccctc gttgcacagt ttaaacagcg aggaggagcg 13201 cattttgcgc tacgtgcagc agagcgtgag ccttaacctg atgcgcgacg gggtaacgcc 13261 cagcgtggcg ctggacatga ccgcgcgcaa catggaaccg ggcatgtatg cctcaaaccg 13321 gccgtttatc aaccgcctaa tggactactt gcatcgcgcg gccgccgtga accccgagta 13381 tttcaccaat gccatcttga acccgcactg gctaccgccc cctggtttct acaccggggg 13441 attcgaggtg cccgagggta acgatggatt cctctgggac gacatagacg acagcgtgtt 13501 ttccccgcaa ccgcagaccc tgctagagtt gcaacagcgc gagcaggcag aggcggcgct 13561 gcgaaaggaa agcttccgca ggccaagcag cttgtccgat ctaggcgctg cggccccgcg 13621 gtcagatgct agtagcccat ttccaagctt gatagggtct cttaccagca ctcgcaccac 13681 ccgcccgcgc ctgctgggcg aggaggagta cctaaacaac tcgctgctgc agccgcagcg 13741 cgaaaaaaac ctgcctccgg catttcccaa caacgggata gagagcctag tggacaagat 13801 gagtagatgg aagacgtacg cgcaggagca cagggacgtg ccaggcccgc gcccgcccac 13861 ccgtcgtcaa aggcacgacc gtcagcgggg tctggtgtgg gaggacgatg actcggcaga 13921 cgacagcagc gtcctggatt tgggagggag tggcaacccg tttgcgcacc ttcgccccag 13981 gctggggaga atgttttaaa aaaaaaaaaa gcatgatgca aaataaaaaa ctcaccaagg 14041 ccatggcacc gagcgttggt tttcttgtat tccccttagt atgcggcgcg cggcgatgta 14101 tgaggaaggt cctcctccct cctacgagag tgtggtgagc gcggcgccag tggcggcggc 14161 gctgggttct cccttcgatg ctcccctgga cccgccgttt gtgcctccgc ggtacctgcg 14221 gcctaccggg gggagaaaca gcatccgtta ctctgagttg gcacccctat tcgacaccac 14281 ccgtgtgtac ctggtggaca acaagtcaac ggatgtggca tccctgaact accagaacga 14341 ccacagcaac tttctgacca cggtcattca aaacaatgac tacagcccgg gggaggcaag 14401 cacacagacc atcaatcttg acgaccggtc gcactggggc ggcgacctga aaaccatcct 14461 gcataccaac atgccaaatg tgaacgagtt catgtttacc aataagttta aggcgcgggt 14521 gatggtgtcg cgcttgccta ctaaggacaa tcaggtggag ctgaaatacg agtgggtgga 14581 gttcacgctg cccgagggca actactccga gaccatgacc atagacctta tgaacaacgc 14641 gatcgtggag cactacttga aagtgggcag acagaacggg gttctggaaa gcgacatcgg 14701 ggtaaagttt gacacccgca acttcagact ggggtttgac cccgtcactg gtcttgtcat 14761 gcctggggta tatacaaacg aagccttcca tccagacatc attttgctgc caggatgcgg 14821 ggtggacttc acccacagcc gcctgagcaa cttgttgggc atccgcaagc ggcaaccctt 14881 ccaggagggc tttaggatca cctacgatga tctggagggt ggtaacattc ccgcactgtt 14941 ggatgtggac gcctaccagg cgagcttgaa agatgacacc gaacagggcg ggggtggcgc 15001 aggcggcagc aacagcagtg gcagcggcgc ggaagagaac tccaacgcgg cagccgcggc 15061 aatgcagccg gtggaggaca tgaacgatca tgccattcgc ggcgacacct ttgccacacg 15121 ggctgaggag aagcgcgctg aggccgaagc agcggccgaa gctgccgccc ccgctgcgca 15181 acccgaggtc gagaagcctc agaagaaacc ggtgatcaaa cccctgacag aggacagcaa 15241 gaaacgcagt tacaacctaa taagcaatga cagcaccttc acccagtacc gcagctggta 15301 ccttgcatac aactacggcg accctcagac cggaatccgc tcatggaccc tgctttgcac 15361 tcctgacgta acctgcggct cggagcaggt ctactggtcg ttgccagaca tgatgcaaga 15421 ccccgtgacc ttccgctcca cgcgccagat cagcaacttt ccggtggtgg gcgccgagct 15481 gttgcccgtg cactccaaga gcttctacaa cgaccaggcc gtctactccc aactcatccg 15541 ccagtttacc tctctgaccc acgtgttcaa tcgctttccc gagaaccaga ttttggcgcg 15601 cccgccagcc cccaccatca ccaccgtcag tgaaaacgtt cctgctctca cagatcacgg 15661 gacgctaccg ctgcgcaaca gcatcggagg agtccagcga gtgaccatta ctgacgccag 15721 acgccgcacc tgcccctacg tttacaaggc cctgggcata gtctcgccgc gcgtcctatc 15781 gagccgcact ttttgagcaa gcatgtccat ccttatatcg cccagcaata acacaggctg 15841 gggcctgcgc ttcccaagca agatgtttgg cggggccaag aagcgctccg accaacaccc 15901 agtgcgcgtg cgcgggcact accgcgcgcc ctggggcgcg cacaaacgcg gccgcactgg 15961 gcgcaccacc gtcgatgacg ccatcgacgc ggtggtggag gaggcgcgca actacacgcc 16021 cacgccgcca ccagtgtcca cagtggacgc ggccattcag accgtggtgc gcggagcccg 16081 gcgctatgct aaaatgaaga gacggcggag gcgcgtagca cgtcgccacc gccgccgacc 16141 cggcactgcc gcccaacgcg cggcggcggc cctgcttaac cgcgcacgtc gcaccggccg 16201 acgggcggcc atgcgggccg ctcgaaggct ggccgcgggt attgtcactg tgccccccag 16261 gtccaggcga cgagcggccg ccgcagcagc cgcggccatt agtgctatga ctcagggtcg 16321 caggggcaac gtgtattggg tgcgcgactc ggttagcggc ctgcgcgtgc ccgtgcgcac 16381 ccgccccccg cgcaactaga ttgcaagaaa aaactactta gactcgtact gttgtatgta 16441 tccagcggcg gcggcgcgca acgaagctat gtccaagcgc aaaatcaaag aagagatgct 16501 ccaggtcatc gcgccggaga tctatggccc cccgaagaag gaagagcagg attacaagcc 16561 ccgaaagcta aagcgggtca aaaagaaaaa gaaagatgat gatgatgaac ttgacgacga 16621 ggtggaactg ctgcacgcta ccgcgcccag gcgacgggta cagtggaaag gtcgacgcgt 16681 aaaacgtgtt ttgcgacccg gcaccaccgt agtctttacg cccggtgagc gctccacccg 16741 cacctacaag cgcgtgtatg atgaggtgta cggcgacgag gacctgcttg agcaggccaa 16801 cgagcgcctc ggggagtttg cctacggaaa gcggcataag gacatgctgg cgttgccgct 16861 ggacgagggc aacccaacac ctagcctaaa gcccgtaaca ctgcagcagg tgctgcccgc 16921 gcttgcaccg tccgaagaaa agcgcggcct aaagcgcgag tctggtgact tggcacccac 16981 cgtgcagctg atggtaccca agcgccagcg actggaagat gtcttggaaa aaatgaccgt 17041 ggaacctggg ctggagcccg aggtccgcgt gcggccaatc aagcaggtgg cgccgggact 17101 gggcgtgcag accgtggacg ttcagatacc cactaccagt agcaccagta ttgccaccgc 17161 cacagagggc atggagacac aaacgtcccc ggttgcctca gcggtggcgg atgccgcggt 17221 gcaggcggtc gctgcggccg cgtccaagac ctctacggag gtgcaaacgg acccgtggat 17281 gtttcgcgtt tcagcccccc ggcgcccgcg ccgttcgagg aagtacggcg ccgccagcgc 17341 gctactgccc gaatatgccc tacatccttc cattgcgcct acccccggct atcgtggcta 17401 cacctaccgc cccagaagac gagcaactac ccgacgccga accaccactg gaacccgccg 17461 ccgccgtcgc cgtcgccagc ccgtgctggc cccgatttcc gtgcgcaggg tggctcgcga 17521 aggaggcagg accctggtgc tgccaacagc gcgctaccac cccagcatcg tttaaaagcc 17581 ggtctttgtg gttcttgcag atatggccct cacctgccgc ctccgtttcc cggtgccggg 17641 attccgagga agaatgcacc gtaggagggg catggccggc cacggcctga cgggcggcat 17701 gcgtcgtgcg caccaccggc ggcggcgcgc gtcgcaccgt cgcatgcgcg gcggtatcct 17761 gcccctcctt attccactga tcgccgcggc gattggcgcc gtgcccggaa ttgcatccgt 17821 ggccttgcag gcgcagagac actgattaaa aacaagttgc atgtggaaaa atcaaaataa 17881 aaagtctgga ctctcacgct cgcttggtcc tgtaactatt ttgtagaatg gaagacatca 17941 actttgcgtc tctggccccg cgacacggct cgcgcccgtt catgggaaac tggcaagata 18001 tcggcaccag caatatgagc ggtggcgcct tcagctgggg ctcgctgtgg agcggcatta 18061 aaaatttcgg ttccaccgtt aagaactatg gcagcaaggc ctggaacagc agcacaggcc 18121 agatgctgag ggataagttg aaagagcaaa atttccaaca aaaggtggta gatggcctgg 18181 cctctggcat tagcggggtg gtggacctgg ccaaccaggc agtgcaaaat aagattaaca 18241 gtaagcttga tccccgccct cccgtagagg agcctccacc ggccgtggag acagtgtctc 18301 cagaggggcg tggcgaaaag cgtccgcgcc ccgacaggga agaaactctg gtgacgcaaa 18361 tagacgagcc tccctcgtac gaggaggcac taaagcaagg cctgcccacc acccgtccca 18421 tcgcgcccat ggctaccgga gtgctgggcc agcacacacc cgtaacgctg gacctgcctc 18481 cccccgccga cacccagcag aaacctgtgc tgccaggccc gaccgccgtt gttgtaaccc 18541 gtcctagccg cgcgtccctg cgccgcgccg ccagcggtcc gcgatcgttg cggcccgtag 18601 ccagtggcaa ctggcaaagc acactgaaca gcatcgtggg tctgggggtg caatccctga 18661 agcgccgacg atgcttctga tagctaacgt gtcgtatgtg tgtcatgtat gcgtccatgt 18721 cgccgccaga ggagctgctg agccgccgcg cgcccgcttt ccaagatggc taccccttcg 18781 atgatgccgc agtggtctta catgcacatc tcgggccagg acgcctcgga gtacctgagc 18841 cccgggctgg tgcagtttgc ccgcgccacc gagacgtact tcagcctgaa taacaagttt 18901 agaaacccca cggtggcgcc tacgcacgac gtgaccacag accggtccca gcgtttgacg 18961 ctgcggttca tccctgtgga ccgtgaggat actgcgtact cgtacaaggc gcggttcacc 19021 ctagctgtgg gtgataaccg tgtgctggac atggcttcca cgtactttga catccgcggc 19081 gtgctggaca ggggccctac ttttaagccc tactctggca ctgcctacaa cgccctggct 19141 cccaagggtg ccccaaatcc ttgcgaatgg gatgaagctg ctactgctct tgaaataaac 19201 ctagaagaag aggacgatga caacgaagac gaagtagacg agcaagctga gcagcaaaaa 19261 actcacgtat ttgggcaggc gccttattct ggtataaata ttacaaagga gggtattcaa 19321 ataggtgtcg aaggtcaaac acctaaatat gccgataaaa catttcaacc tgaacctcaa 19381 ataggagaat ctcagtggta cgaaacagaa attaatcatg cagctgggag agtcctaaaa 19441 aagactaccc caatgaaacc atgttacggt tcatatgcaa aacccacaaa tgaaaatgga 19501 gggcaaggca ttcttgtaaa gcaacaaaat ggaaagctag aaagtcaagt ggaaatgcaa 19561 tttttctcaa ctactgaggc agccgcaggc aatggtgata acttgactcc taaagtggta 19621 ttgtacagtg aagatgtaga tatagaaacc ccagacactc atatttctta catgcccact 19681 attaaggaag gtaactcacg agaactaatg ggccaacaat ctatgcccaa caggcctaat 19741 tacattgctt ttagggacaa ttttattggt ctaatgtatt acaacagcac gggtaatatg 19801 ggtgttctgg cgggccaagc atcgcagttg aatgctgttg tagatttgca agacagaaac 19861 acagagcttt cataccagct tttgcttgat tccattggtg atagaaccag gtacttttct 19921 atgtggaatc aggctgttga cagctatgat ccagatgtta gaattattga aaatcatgga 19981 actgaagatg aacttccaaa ttactgcttt ccactgggag gtgtgattaa tacagagact 20041 cttaccaagg taaaacctaa aacaggtcag gaaaatggat gggaaaaaga tgctacagaa 20101 ttttcagata aaaatgaaat aagagttgga aataattttg ccatggaaat caatctaaat 20161 gccaacctgt ggagaaattt cctgtactcc aacatagcgc tgtatttgcc cgacaagcta 20221 aagtacagtc cttccaacgt aaaaatttct gataacccaa acacctacga ctacatgaac 20281 aagcgagtgg tggctcccgg gctagtggac tgctacatta accttggagc acgctggtcc 20341 cttgactata tggacaacgt caacccattt aaccaccacc gcaatgctgg cctgcgctac 20401 cgctcaatgt tgctgggcaa tggtcgctat gtgcccttcc acatccaggt gcctcagaag 20461 ttctttgcca ttaaaaacct ccttctcctg ccgggctcat acacctacga gtggaacttc 20521 aggaaggatg ttaacatggt tctgcagagc tccctaggaa atgacctaag ggttgacgga 20581 gccagcatta agtttgatag catttgcctt tacgccacct tcttccccat ggcccacaac 20641 accgcctcca cgcttgaggc catgcttaga aacgacacca acgaccagtc ctttaacgac 20701 tatctctccg ccgccaacat gctctaccct atacccgcca acgctaccaa cgtgcccata 20761 tccatcccct cccgcaactg ggcggctttc cgcggctggg ccttcacgcg ccttaagact 20821 aaggaaaccc catcactggg ctcgggctac gacccttatt acacctactc tggctctata 20881 ccctacctag atggaacctt ttacctcaac cacaccttta agaaggtggc cattaccttt 20941 gactcttctg tcagctggcc tggcaatgac cgcctgctta cccccaacga gtttgaaatt 21001 aagcgctcag ttgacgggga gggttacaac gttgcccagt gtaacatgac caaagactgg 21061 ttcctggtac aaatgctagc taactataac attggctacc agggcttcta tatcccagag 21121 agctacaagg accgcatgta ctccttcttt agaaacttcc agcccatgag ccgtcaggtg 21181 gtggatgata ctaaatacaa ggactaccaa caggtgggca tcctacacca acacaacaac 21241 tctggatttg ttggctacct tgcccccacc atgcgcgaag gacaggccta ccctgctaac 21301 ttcccctatc cgcttatagg caagaccgca gttgacagca ttacccagaa aaagtttctt 21361 tgcgatcgca ccctttggcg catcccattc tccagtaact ttatgtccat gggcgcactc 21421 acagacctgg gccaaaacct tctctacgcc aactccgccc acgcgctaga catgactttt 21481 gaggtggatc ccatggacga gcccaccctt ctttatgttt tgtttgaagt ctttgacgtg 21541 gtccgtgtgc accagccgca ccgcggcgtc atcgaaaccg tgtacctgcg cacgcccttc 21601 tcggccggca acgccacaac ataaagaagc aagcaacatc aacaacagct gccgccaaat 21661 aatgtactag agacactttc aataaaggca aatgctttta tttgtacact ctcgggtgat 21721 tatttacccc cacccttgcc gtctgcgccg tttaaaaatc aaaggggttc tgccgcgcat 21781 cgctatgcgc cactggcagg gacacgttgc gatactggtg tttagtgctc cacttaaact 21841 caggcacaac catccgcggc agctcggtga agttttcact ccacaggctg cgcaccatca 21901 ccaacgcgtt tagcaggtcg ggcgccgata tcttgaagtc gcagttgggg cctccgccct 21961 gcgcgcgcga gttgcgatac acagggttgc agcactggaa cactatcagc gccgggtggt 22021 gcacgctggc cagcacgctc ttgtcggaga tcagatccgc gtccaggtcc tccgcgttgc 22081 tcagggcgaa cggagtcaac tttggtagct gccttcccaa aaagggcgcg tgcccaggct 22141 ttgagttgca ctcgcaccgt agtggcatca aaaggtgacc gtgcccggtc tgggcgttag 22201 gatacagcgc ctgcataaaa gccttgatct gcttaaaagc cacctgagcc tttgcgcctt 22261 cagagaagaa catgccgcaa gacttgccgg aaaactgatt ggccggacag gccgcgtcgt 22321 gcacgcagca ccttgcgtcg gtgttggaga tctgcaccac atttcggccc caccggttct 22381 tcacgatctt ggccttgcta gactgctcct tcagcgcgcg ctgcccgttt tcgctcgtca 22441 catccatttc aatcacgtgc tccttattta tcataatgct tccgtgtaga cacttaagct 22501 cgccttcgat ctcagcgcag cggtgcagcc acaacgcgca gcccgtgggc tcgtgatgct 22561 tgtaggtcac ctctgcaaac gactgcaggt acgcctgcag gaatcgcccc atcatcgtca 22621 caaaggtctt gttgctggtg aaggtcagct gcaacccgcg gtgctcctcg ttcagccagg 22681 tcttgcatac ggccgccaga gcttccactt ggtcaggcag tagtttgaag ttcgccttta 22741 gatcgttatc cacgtggtac ttgtccatca gcgcgcgcgc agcctccatg cccttctccc 22801 acgcagacac gatcggcaca ctcagcgggt tcatcaccgt aatttcactt tccgcttcgc 22861 tgggctcttc ctcttcctct tgcgtccgca taccacgcgc cactgggtcg tcttcattca 22921 gccgccgcac tgtgcgctta cctcctttgc catgcttgat tagcaccggt gggttgctga 22981 aacccaccat ttgtagcgcc acatcttctc tttcttcctc gctgtccacg attacctctg 23041 gtgatggcgg gcgctcgggc ttgggagaag ggcgcttctt tttcttcttg ggcgcaatgg 23101 ccaaatccgc cgccgaggtc gatggccgcg ggctgggtgt gcgcggcacc agcgcgtctt 23161 gtgatgagtc ttcctcgtcc tcggactcga tacgccgcct catccgcttt tttgggggcg 23221 cccggggagg cggcggcgac ggggacgggg acgacacgtc ctccatggtt gggggacgtc 23281 gcgccgcacc gcgtccgcgc tcgggggtgg tttcgcgctg ctcctcttcc cgactggcca 23341 tttccttctc ctataggcag aaaaagatca tggagtcagt cgagaagaag gacagcctaa 23401 ccgccccctc tgagttcgcc accaccgcct ccaccgatgc cgccaacgcg cctaccacct 23461 tccccgtcga ggcacccccg cttgaggagg aggaagtgat tatcgagcag gacccaggtt 23521 ttgtaagcga agacgacgag gaccgctcag taccaacaga ggataaaaag caagaccagg 23581 acaacgcaga ggcaaacgag gaacaagtcg ggcgggggga cgaaaggcat ggcgactacc 23641 tagatgtggg agacgacgtg ctgttgaagc atctgcagcg ccagtgcgcc attatctgcg 23701 acgcgttgca agagcgcagc gatgtgcccc tcgccatagc ggatgtcagc cttgcctacg 23761 aacgccacct attctcaccg cgcgtacccc ccaaacgcca agaaaacggc acatgcgagc 23821 ccaacccgcg cctcaacttc taccccgtat ttgccgtgcc agaggtgctt gccacctatc 23881 acatcttttt ccaaaactgc aagatacccc tatcctgccg tgccaaccgc agccgagcgg 23941 acaagcagct ggccttgcgg cagggcgctg tcatacctga tatcgcctcg ctcaacgaag 24001 tgccaaaaat ctttgagggt cttggacgcg acgagaagcg cgcggcaaac gctctgcaac 24061 aggaaaacag cgaaaatgaa agtcactctg gagtgttggt ggaactcgag ggtgacaacg 24121 cgcgcctagc cgtactaaaa cgcagcatcg aggtcaccca ctttgcctac ccggcactta 24181 acctaccccc caaggtcatg agcacagtca tgagtgagct gatcgtgcgc cgtgcgcagc 24241 ccctggagag ggatgcaaat ttgcaagaac aaacagagga gggcctaccc gcagttggcg 24301 acgagcagct agcgcgctgg cttcaaacgc gcgagcctgc cgacttggag gagcgacgca 24361 aactaatgat ggccgcagtg ctcgttaccg tggagcttga gtgcatgcag cggttctttg 24421 ctgacccgga gatgcagcgc aagctagagg aaacattgca ctacaccttt cgacagggct 24481 acgtacgcca ggcctgcaag atctccaacg tggagctctg caacctggtc tcctaccttg 24541 gaattttgca cgaaaaccgc cttgggcaaa acgtgcttca ttccacgctc aagggcgagg 24601 cgcgccgcga ctacgtccgc gactgcgttt acttatttct atgctacacc tggcagacgg 24661 ccatgggcgt ttggcagcag tgcttggagg agtgcaacct caaggagctg cagaaactgc 24721 taaagcaaaa cttgaaggac ctatggacgg ccttcaacga gcgctccgtg gccgcgcacc 24781 tggcggacat cattttcccc gaacgcctgc ttaaaaccct gcaacagggt ctgccagact 24841 tcaccagtca aagcatgttg cagaacttta ggaactttat cctagagcgc tcaggaatct 24901 tgcccgccac ctgctgtgca cttcctagcg actttgtgcc cattaagtac cgcgaatgcc 24961 ctccgccgct ttggggccac tgctaccttc tgcagctagc caactacctt gcctaccact 25021 ctgacataat ggaagacgtg agcggtgacg gtctactgga gtgtcactgt cgctgcaacc 25081 tatgcacccc gcaccgctcc ctggtttgca attcgcagct gcttaacgaa agtcaaatta 25141 tcggtacctt tgagctgcag ggtccctcgc ctgacgaaaa gtccgcggct ccggggttga 25201 aactcactcc ggggctgtgg acgtcggctt accttcgcaa atttgtacct gaggactacc 25261 acgcccacga gattaggttc tacgaagacc aatcccgccc gcctaatgcg gagcttaccg 25321 cctgcgtcat tacccagggc cacattcttg gccaattgca agccatcaac aaagcccgcc 25381 aagagtttct gctacgaaag ggacgggggg tttacttgga cccccagtcc ggcgaggagc 25441 tcaacccaat ccccccgccg ccgcagccct atcagcagca gccgcgggcc cttgcttccc 25501 aggatggcac ccaaaaagaa gctgcagctg ccgccgccac ccacggacga ggaggaatac 25561 tgggacagtc aggcagagga ggttttggac gaggaggagg aggacatgat ggaagactgg 25621 gagagcctag acgaggaagc ttccgaggtc gaagaggtgt cagacgaaac accgtcaccc 25681 tcggtcgcat tcccctcgcc ggcgccccag aaatcggcaa ccggttccag catggctaca 25741 acctccgctc ctcaggcgcc gccggcactg cccgttcgcc gacccaaccg tagatgggac 25801 accactggaa ccagggccgg taagtccaag cagccgccgc cgttagccca agagcaacaa 25861 cagcgccaag gctaccgctc atggcgcggg cacaagaacg ccatagttgc ttgcttgcaa 25921 gactgtgggg gcaacatctc cttcgcccgc cgctttcttc tctaccatca cggcgtggcc 25981 ttcccccgta acatcctgca ttactaccgt catctctaca gcccatactg caccggcggc 26041 agcggcagca acagcagcgg ccacacagaa gcaaaggcga ccggatagca agactctgac 26101 aaagcccaag aaatccacag cggcggcagc agcaggagga ggagcgctgc gtctggcgcc 26161 caacgaaccc gtatcgaccc gcgagcttag aaacaggatt tttcccactc tgtatgctat 26221 atttcaacag agcaggggcc aagaacaaga gctgaaaata aaaaacaggt ctctgcgatc 26281 cctcacccgc agctgcctgt atcacaaaag cgaagatcag cttcggcgca cgctggaaga 26341 cgcggaggct ctcttcagta aatactgcgc gctgactctt aaggactagt ttcgcgccct 26401 ttctcaaatt taagcgcgaa aactacgtca tctccagcgg ccacacccgg cgccagcacc 26461 tgttgtcagc gccattatga gcaaggaaat tcccacgccc tacatgtgga gttaccagcc 26521 acaaatggga cttgcggctg gagctgccca agactactca acccgaataa actacatgag 26581 cgcgggaccc cacatgatat cccgggtcaa cggaatacgc gcccaccgaa accgaattct 26641 cctggaacag gcggctatta ccaccacacc tcgtaataac cttaatcccc gtagttggcc 26701 cgctgccctg gtgtaccagg aaagtcccgc tcccaccact gtggtacttc ccagagacgc 26761 ccaggccgaa gttcagatga ctaactcagg ggcgcagctt gcgggcggct ttcgtcacag 26821 ggtgcggtcg cccgggcagg gtataactca cctgacaatc agagggcgag gtattcagct 26881 caacgacgag tcggtgagct cctcgcttgg tctccgtccg gacgggacat ttcagatcgg 26941 cggcgccggc cgctcttcat tcacgcctcg tcaggcaatc ctaactctgc agacctcgtc 27001 ctctgagccg cgctctggag gcattggaac tctgcaattt attgaggagt ttgtgccatc 27061 ggtctacttt aaccccttct cgggacctcc cggccactat ccggatcaat ttattcctaa 27121 ctttgacgcg gtaaaggact cggcggacgg ctacgactga atgttaagtg gagaggcaga 27181 gcaactgcgc ctgaaacacc tggtccactg tcgccgccac aagtgctttg cccgcgactc 27241 cggtgagttt tgctactttg aattgcccga ggatcatatc gagggcccgg cgcacggcgt 27301 ccggcttacc gcccagggag agcttgcccg tagcctgatt cgggagttta cccagcgccc 27361 cctgctagtt gagcgggaca ggggaccctg tgttctcact gtgatttgca actgtcctaa 27421 ccctggatta catcaagatc tttgttgcca tctctgtgct gagtataata aatacagaaa 27481 ttaaaatata ctggggctcc tatcgccatc ctgtaaacgc caccgtcttc acccgcccaa 27541 gcaaaccaag gcgaacctta cctggtactt ttaacatctc tccctctgtg atttacaaca 27601 gtttcaaccc agacggagtg agtctacgag agaacctctc cgagctcagc tactccatca 27661 gaaaaaacac caccctcctt acctgccggg aacgtacgag tgcgtcaccg gccgctgcac 27721 cacacctacc gcctgaccgt aaaccagact ttttccggac agacctcaat aactctgttt 27781 accagaacag gaggtgagct tagaaaaccc ttagggtatt aggccaaagg cgcagctact 27841 gtggggttta tgaacaattc aagcaactct acgggctatt ctaattcagg tttctctaga 27901 aatggacgga attattacag agcagcgcct gctagaaaga cgcagggcag cggccgagca 27961 acagcgcatg aatcaagagc tccaagacat ggttaacttg caccagtgca aaaggggtat 28021 cttttgtctg gtaaagcagg ccaaagtcac ctacgacagt aataccaccg gacaccgcct 28081 tagctacaag ttgccaacca agcgtcagaa attggtggtc atggtgggag aaaagcccat 28141 taccataact cagcactcgg tagaaaccga aggctgcatt cactcacctt gtcaaggacc 28201 tgaggatctc tgcaccctta ttaagaccct gtgcggtctc aaagatctta ttccctttaa 28261 ctaataaaaa aaaataataa agcatcactt acttaaaatc agttagcaaa tttctgtcca 28321 gtttattcag cagcacctcc ttgccctcct cccagctctg gtattgcagc ttcctcctgg 28381 ctgcaaactt tctccacaat ctaaatggaa tgtcagtttc ctcctgttcc tgtccatccg 28441 cacccactat cttcatgttg ttgcagatga agcgcgcaag accgtctgaa gataccttca 28501 accccgtgta tccatatgac acggaaaccg gtcctccaac tgtgcctttt cttactcctc 28561 cctttgtatc ccccaatggg tttcaagaga gtccccctgg ggtactctct ttgcgcctat 28621 ccgaacctct agttacctcc aatggcatgc ttgcgctcaa aatgggcaac ggcctctctc 28681 tggacgaggc cggcaacctt acctcccaaa atgtaaccac tgtgagccca cctctcaaaa 28741 aaaccaagtc aaacataaac ctggaaatat ctgcacccct cacagttacc tcagaagccc 28801 taactgtggc tgccgccgca cctctaatgg tcgcgggcaa cacactcacc atgcaatcac 28861 aggccccgct aaccgtgcac gactccaaac ttagcattgc cacccaagga cccctcacag 28921 tgtcagaagg aaagctagcc ctgcaaacat caggccccct caccaccacc gatagcagta 28981 cccttactat cactgcctca ccccctctaa ctactgccac tggtagcttg ggcattgact 29041 tgaaagagcc catttataca caaaatggaa aactaggact aaagtacggg gctcctttgc 29101 atgtaacaga cgacctaaac actttgaccg tagcaactgg tccaggtgtg actattaata 29161 atacttcctt gcaaactaaa gttactggag ccttgggttt tgattcacaa ggcaatatgc 29221 aacttaatgt agcaggagga ctaaggattg attctcaaaa cagacgcctt atacttgatg 29281 ttagttatcc gtttgatgct caaaaccaac taaatctaag actaggacag ggccctcttt 29341 ttataaactc agcccacaac ttggatatta actacaacaa aggcctttac ttgtttacag 29401 cttcaaacaa ttccaaaaag cttgaggtta acctaagcac tgccaagggg ttgatgtttg 29461 acgctacagc catagccatt aatgcaggag atgggcttga atttggttca cctaatgcac 29521 caaacacaaa tcccctcaaa acaaaaattg gccatggcct agaatttgat tcaaacaagg 29581 ctatggttcc taaactagga actggcctta gttttgacag cacaggtgcc attacagtag 29641 gaaacaaaaa taatgataag ctaactttgt ggaccacacc agctccatct cctaactgta 29701 gactaaatgc agagaaagat gctaaactca ctttggtctt aacaaaatgt ggcagtcaaa 29761 tacttgctac agtttcagtt ttggctgtta aaggcagttt ggctccaata tctggaacag 29821 ttcaaagtgc tcatcttatt ataagatttg acgaaaatgg agtgctacta aacaattcct 29881 tcctggaccc agaatattgg aactttagaa atggagatct tactgaaggc acagcctata 29941 caaacgctgt tggatttatg cctaacctat cagcttatcc aaaatctcac ggtaaaactg 30001 ccaaaagtaa cattgtcagt caagtttact taaacggaga caaaactaaa cctgtaacac 30061 taaccattac actaaacggt acacaggaaa caggagacac aactccaagt gcatactcta 30121 tgtcattttc atgggactgg tctggccaca actacattaa tgaaatattt gccacatcct 30181 cttacacttt ttcatacatt gcccaagaat aaagaatcgt ttgtgttatg tttcaacgtg 30241 tttatttttc aattgcagaa aatttcgaat catttttcat tcagtagtat agccccacca 30301 ccacatagct tatacagatc accgtacctt aatcaaactc acagaaccct agtattcaac 30361 ctgccacctc cctcccaaca cacagagtac acagtccttt ctccccggct ggccttaaaa 30421 agcatcatat catgggtaac agacatattc ttaggtgtta tattccacac ggtttcctgt 30481 cgagccaaac gctcatcagt gatattaata aactccccgg gcagctcact taagttcatg 30541 tcgctgtcca gctgctgagc cacaggctgc tgtccaactt gcggttgctt aacgggcggc 30601 gaaggagaag tccacgccta catgggggta gagtcataat cgtgcatcag gatagggcgg 30661 tggtgctgca gcagcgcgcg aataaactgc tgccgccgcc gctccgtcct gcaggaatac 30721 aacatggcag tggtctcctc agcgatgatt cgcaccgccc gcagcataag gcgccttgtc 30781 ctccgggcac agcagcgcac cctgatctca cttaaatcag cacagtaact gcagcacagc 30841 accacaatat tgttcaaaat cccacagtgc aaggcgctgt atccaaagct catggcgggg 30901 accacagaac ccacgtggcc atcataccac aagcgcaggt agattaagtg gcgacccctc 30961 ataaacacgc tggacataaa cattacctct tttggcatgt tgtaattcac cacctcccgg 31021 taccatataa acctctgatt aaacatggcg ccatccacca ccatcctaaa ccagctggcc 31081 aaaacctgcc cgccggctat acactgcagg gaaccgggac tggaacaatg acagtggaga 31141 gcccaggact cgtaaccatg gatcatcatg ctcgtcatga tatcaatgtt ggcacaacac 31201 aggcacacgt gcatacactt cctcaggatt acaagctcct cccgcgttag aaccatatcc 31261 cagggaacaa cccattcctg aatcagcgta aatcccacac tgcagggaag acctcgcacg 31321 taactcacgt tgtgcattgt caaagtgtta cattcgggca gcagcggatg atcctccagt 31381 atggtagcgc gggtttctgt ctcaaaagga ggtagacgat ccctactgta cggagtgcgc 31441 cgagacaacc gagatcgtgt tggtcgtagt gtcatgccaa atggaacgcc ggacgtagtc 31501 atatttcctg aagcaaaacc aggtgcgggc gtgacaaaca gatctgcgtc tccggtctcg 31561 ccgcttagat cgctctgtgt agtagttgta gtatatccac tctctcaaag catccaggcg 31621 ccccctggct tcgggttcta tgtaaactcc ttcatgcgcc gctgccctga taacatccac 31681 caccgcagaa taagccacac ccagccaacc tacacattcg ttctgcgagt cacacacggg 31741 aggagcggga agagctggaa gaaccatgtt ttttttttta ttccaaaaga ttatccaaaa 31801 cctcaaaatg aagatctatt aagtgaacgc gctcccctcc ggtggcgtgg tcaaactcta 31861 cagccaaaga acagataatg gcatttgtaa gatgttgcac aatggcttcc aaaaggcaaa 31921 cggccctcac gtccaagtgg acgtaaaggc taaacccttc agggtgaatc tcctctataa 31981 acattccagc accttcaacc atgcccaaat aattctcatc tcgccacctt ctcaatatat 32041 ctctaagcaa atcccgaata ttaagtccgg ccattgtaaa aatctgctcc agagcgccct 32101 ccaccttcag cctcaagcag cgaatcatga ttgcaaaaat tcaggttcct cacagacctg 32161 tataagattc aaaagcggaa cattaacaaa aataccgcga tcccgtaggt cccttcgcag 32221 ggccagctga acataatcgt gcaggtctgc acggaccagc gcggccactt ccccgccagg 32281 aaccatgaca aaagaaccca cactgattat gacacgcata ctcggagcta tgctaaccag 32341 cgtagccccg atgtaagctt gttgcatggg cggcgatata aaatgcaagg tgctgctcaa 32401 aaaatcaggc aaagcctcgc gcaaaaaaga aagcacatcg tagtcatgct catgcagata 32461 aaggcaggta agctccggaa ccaccacaga aaaagacacc atttttctct caaacatgtc 32521 tgcgggtttc tgcataaaca caaaataaaa taacaaaaaa acatttaaac attagaagcc 32581 tgtcttacaa caggaaaaac aacccttata agcataagac ggactacggc catgccggcg 32641 tgaccgtaaa aaaactggtc accgtgatta aaaagcacca ccgacagctc ctcggtcatg 32701 tccggagtca taatgtaaga ctcggtaaac acatcaggtt gattcacatc ggtcagtgct 32761 aaaaagcgac cgaaatagcc cgggggaata catacccgca ggcgtagaga caacattaca 32821 gcccccatag gaggtataac aaaattaata ggagagaaaa acacataaac acctgaaaaa 32881 ccctcctgcc taggcaaaat agcaccctcc cgctccagaa caacatacag cgcttccaca 32941 gcggcagcca taacagtcag ccttaccagt aaaaaagaaa acctattaaa aaaacaccac 33001 tcgacacggc accagctcaa tcagtcacag tgtaaaaaag ggccaagtgc agagcgagta 33061 tatataggac taaaaaatga cgtaacggtt aaagtccaca aaaaacaccc agaaaaccgc 33121 acgcgaacct acgcccagaa acgaaagcca aaaaacccac aacttcctca aatcgtcact 33181 tccgttttcc cacgttactt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 33241 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 33301 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 33361 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 33421 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg 33481 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaac 33541 ctccgcgggg atccgcacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa 33601 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat 33661 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac 33721 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc 33781 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga 33841 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca 33901 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt 33961 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg 34021 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga 34081 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct 34141 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac 34201 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct 34261 gtacaagtag ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 34321 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 34381 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat 34441 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 34501 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 34561 tataccgtcg acctctagct agaggtcact tcccatttta agaaaactac aattcccaac 34621 acatacaagt tactccgccc taaaacctac gtcacccgcc ccgttcccac gccccgcgcc 34681 acgtcacaaa ctccaccccc tcattatcat attggcttca atccaaaata aggtatatta 34741 ttgatgatgt taattaattt aaatccgcat gcgatatcga gctctcccgg gaattcggat 34801 ctgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg 34861 ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc 34921 ttcaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 34981 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 35041 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 35101 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 35161 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 35221 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 35281 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 35341 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 35401 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 35461 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 35521 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 35581 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 35641 tatcaaaaag gatcttcacc tagatccttt taaatcaatc taaagtatat atgagtaaac 35701 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 35761 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 35821 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 35881 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 35941 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 36001 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 36061 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 36121 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 36181 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 36241 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 36301 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac 36361 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 36421 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 36481 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 36541 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 36601 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 36661 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 36721 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga 36781 attggatccg aattcccggg agagctcgat atcgcatgcg gatttaaatt aattaa //
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