质粒名称: | pLemir9-124 |
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出品公司: | 爱迪基因 |
目录编号: | 31779 |
存储实验室: | Jerry Crabtree |
载体骨架基本信息 |
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载体名称: | pLemir |
载体出品公司: | Open Biosystems |
载体抗性: | 氨苄霉素 |
空载体大小: | 11315 bp |
载体类型: | 慢病毒表达 |
筛选标记: | 嘌呤霉素 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | miR-9/9* and miR-124 |
物种来源: | M. musculus (鼠) |
插入大小: | 655 bp |
GenBank编号 | NR_029818 NR_029814 |
克隆方法: | Ligation单克隆 |
5' 克隆位点: | |
3' 克隆位点: | |
5' 测序引物 | CACTTCGTGGACCACAGACTGG |
3' 测序引物 | GCACACCGGCCTTATTCCAAG |
文献引用方法 |
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材料和方法部分: | pLemir9-124 was a gift from Jerry Crabtree (Addgene plasmid # 31779) |
参考文献部分: | MicroRNA-mediated conversion of human fibroblasts to neurons. Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer C, Dolmetsch RE, Tsien RW, Crabtree GR. Nature. 2011 Jul 13. doi: 10.1038/nature10323. 10.1038/nature10323 PubMed 21753754 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLemir9-124 11983 bp DNA SYN DEFINITION pLemir9-124 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..11983 /organism="pLemir9-124" /mol_type="other DNA" misc_feature 454..634 /label="HIV-1_5_LTR" misc_feature 454..634 /label="truncHIV-1_3_LTR" misc_feature 745..789 /label="HIV-1_psi_pack" CDS complement(1015..1476) /label="ORF frame 2" CDS 1181..2068 /label="ORF frame 2" misc_feature 1303..1536 /label="RRE" misc_feature 2067..2082 /label="cPPT" misc_feature complement(2245..2619) /label="sh_ble" gene complement(2248..2619) /label="bleo" /gene="bleo" promoter complement(2629..2647) /label="T7_promoter" promoter 2750..3254 /label="CMV_immearly_promoter" misc_feature 2769..3044 /label="CAG_enhancer" misc_feature 3211..3231 /label="CMV_fwd_primer" promoter 3212..3281 /label="CMV_promoter" misc_feature 3255..3274 /label="pCEP_fwd_primer" misc_feature 3257..3281 /label="LNCX_primer" CDS 3364..4059 /label="ORF frame 1" misc_feature 4760..5323 /label="IRES" CDS 5336..5938 /label="ORF frame 2" gene 5351..5938 /label="puro (variant)" /gene="puro (variant)" misc_feature 5956..6531 /label="WPRE" CDS 6044..6571 /label="ORF frame 2" misc_feature 6749..6801 /label="delta_U3" misc_feature 6802..6982 /label="HIV-1_5_LTR" misc_feature 6802..6982 /label="truncHIV-1_3_LTR" misc_feature complement(7083..7100) /label="BGH_rev_primer" terminator 7086..7313 /label="bGH_PA_terminator" rep_origin 7376..7682 /label="f1_origin" misc_feature complement(7796..7816) /label="pBABE_3_primer" misc_feature complement(7802..8017) /label="SV40_enhancer" promoter 7814..8082 /label="SV40_promoter" rep_origin 7981..8058 /label="SV40_origin" misc_feature 8043..8062 /label="SV40pro_F_primer" gene 8194..9205 /label="hygroB" /gene="hygroB" CDS complement(8288..8941) /label="ORF frame 1" CDS 8537..9208 /label="ORF frame 2" terminator 9341..9460 /label="SV40_PA_terminator" misc_feature 9429..9448 /label="EBV_rev_primer" promoter complement(9504..9522) /label="M13_reverse_primer" misc_feature complement(9521..9543) /label="M13_pUC_rev_primer" promoter complement(9557..9586) /label="lac_promoter" gene complement(10669..11529) /label="Ampicillin" /gene="Ampicillin" CDS complement(10669..11529) /label="ORF frame 2" promoter complement(11571..11599) /label="AmpR_promoter" terminator 11685..11816 /label="SV40_PA_terminator" misc_feature 11773..11792 /label="EBV_rev_primer" ORIGIN 1 Tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac 2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag 2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa 2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag 2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt 2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat 2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag 2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg 2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac 2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg 2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt 2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc 2701 cgaggttcta gagaccggtg gttaacgcgg atcccatcga ttgggagttc cgcgttacat 2761 aacttaccgt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa 2821 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg 2881 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc 2941 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct 3001 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga 3061 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa 3121 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc 3181 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg 3241 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac 3301 gctgttttga cctccataga agacaccgac tctagctaga ggctagcgct accggtcgcc 3361 accatgagcg agctgatcaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg 3421 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag 3481 accatgaaga tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct 3541 accagcttca tgtacggcag caaagccttc atcaaccaca cccagggcat ccccgacttc 3601 tttaagcagt ccttccctga gggcttcaca tgggagagaa tcaccacata cgaagacggg 3661 ggcgtgctga ccgctaccca ggacaccagc ttccagaacg gctgcatcat ctacaacgtc 3721 aagatcaacg gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacacgcggc 3781 tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctgagagg ccacagccag 3841 atggccctga agctcgtggg cgggggctac ctgcactgct ccttcaagac cacatacaga 3901 tccaagaaac ccgctaagaa cctcaagatg cccggcttcc acttcgtgga ccacagactg 3961 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgagat ggctgtggcc 4021 aagtactgcg acctccctag caaactgggg cacagataac tcgagcggac gcgtcggcgg 4081 cctgtcggtc ccctctggct ctccgcgtgc gcccggggct gcgggagcgg agtccgctca 4141 ggcccccgcg ccgggtcggc ggcggcacgt ggggcccaca gcgcggcagc ggtgccggcg 4201 aatgggaggc ccgtttctct ctttggttat ctagctgtat gagtgccaca gagccgtcat 4261 aaagctagat aaccgaaagt agaaatgact ctcacaactt ctgcgtgcga tggcccggcc 4321 ggccgccctg gccacaggag ccagaggagc ccagagaagc ccacgccacc cagggattcc 4381 agctcgacgg agcggccacg ccgggaccag gtatccgacc tcggctctcc ttctagtagg 4441 tgggagtact gctcagagct acaactctag gagtagggac tccaagccta gagctccaag 4501 agagggtgaa gggcagggag aaaattatag taatagttgc aatgagtcac ttgcttctag 4561 atcaagatca gagactctgc tctccgtgtt cacagcggac cttgatttaa tgtcatacaa 4621 ttaaggcacg cggtgaatgc caagagcgga gcctacagct gcacttgaag gacatccgag 4681 agaagttagg aagggtgggg agaaacaatt ctagaatgaa cccatcctgt gcgacacggc 4741 cgcaaattcc gcaaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc 4801 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct 4861 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt 4921 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct 4981 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc 5041 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag 5101 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc 5161 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga 5221 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt 5281 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatggc 5341 caccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg 5401 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg 5461 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 5521 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 5581 cgtcgaagcg ggggcggtgt tcgccgagat cggctcgcgc atggccgagt tgagcggttc 5641 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 5701 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 5761 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgct ggggtgcccg ccttcctgga 5821 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 5881 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt 5941 tcgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 6001 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 6061 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 6121 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 6181 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 6241 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 6301 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 6361 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 6421 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 6481 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 6541 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat 6601 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt 6661 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 6721 agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa 6781 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 6841 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 6901 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 6961 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 7021 cttgcaaaga aatgaatatc agagagtgag aggccttgac attgtttaaa cccgctgatc 7081 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 7141 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 7201 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 7261 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 7321 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 7381 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 7441 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 7501 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 7561 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 7621 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 7681 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 7741 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 7801 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 7861 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 7921 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 7981 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 8041 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 8101 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 8161 ggatctgatc agcacgtgat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt 8221 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct 8281 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc 8341 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt 8401 ccggaagtgc ttgacattgg ggaattaatt cagcgagagc ctgacctatt gcatctcccg 8461 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca 8521 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt 8581 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc 8641 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc 8701 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca 8761 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc 8821 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt 8881 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca 8941 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca 9001 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg 9061 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag 9121 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc 9181 cagcactcgt ccgagggcaa aggaatagca cgtgctacga gatttcgatt ccaccgccgc 9241 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 9301 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 9361 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 9421 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 9481 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 9541 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 9601 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 9661 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 9721 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 9781 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 9841 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 9901 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 9961 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 10021 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 10081 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 10141 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 10201 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 10261 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 10321 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 10381 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 10441 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 10501 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 10561 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 10621 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 10681 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 10741 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 10801 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 10861 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 10921 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 10981 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 11041 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 11101 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 11161 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 11221 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 11281 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 11341 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 11401 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 11461 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 11521 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 11581 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 11641 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt 11701 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 11761 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact 11821 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg 11881 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt 11941 aaagaaattg tatttgttaa atatgtacta caaacttagt agt //