pLemir9-124

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pLemir9-124 
出品公司: 爱迪基因
目录编号: 31779
存储实验室: Jerry Crabtree

载体骨架基本信息
载体名称: pLemir
载体出品公司: Open Biosystems
载体抗性: 氨苄霉素
空载体大小: 11315 bp
载体类型: 慢病毒表达
筛选标记: 嘌呤霉素
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: miR-9/9* and miR-124
物种来源: M. musculus (鼠)
插入大小: 655 bp
GenBank编号 NR_029818 NR_029814
克隆方法: Ligation单克隆
5' 克隆位点:  
3' 克隆位点:  
5' 测序引物 CACTTCGTGGACCACAGACTGG
3' 测序引物 GCACACCGGCCTTATTCCAAG

文献引用方法
材料和方法部分: pLemir9-124 was a gift from Jerry Crabtree (Addgene plasmid # 31779)
参考文献部分: MicroRNA-mediated conversion of human fibroblasts to neurons. Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer C, Dolmetsch RE, Tsien RW, Crabtree GR. Nature. 2011 Jul 13. doi: 10.1038/nature10323. 10.1038/nature10323 PubMed 21753754

载体图谱质粒图谱和多克隆位点信息

pLemir9-124载体图谱

载体简介

载体序列

LOCUS       pLemir9-124	11983 bp 	DNA	SYN
DEFINITION  pLemir9-124
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..11983
                     /organism="pLemir9-124"
                     /mol_type="other DNA"
     misc_feature    454..634
                     /label="HIV-1_5_LTR"
     misc_feature    454..634
                     /label="truncHIV-1_3_LTR"
     misc_feature    745..789
                     /label="HIV-1_psi_pack"
     CDS             complement(1015..1476)
                     /label="ORF frame 2"
     CDS             1181..2068
                     /label="ORF frame 2"
     misc_feature    1303..1536
                     /label="RRE"
     misc_feature    2067..2082
                     /label="cPPT"
     misc_feature    complement(2245..2619)
                     /label="sh_ble"
     gene            complement(2248..2619)
                     /label="bleo"
                     /gene="bleo"
     promoter        complement(2629..2647)
                     /label="T7_promoter"
     promoter        2750..3254
                     /label="CMV_immearly_promoter"
     misc_feature    2769..3044
                     /label="CAG_enhancer"
     misc_feature    3211..3231
                     /label="CMV_fwd_primer"
     promoter        3212..3281
                     /label="CMV_promoter"
     misc_feature    3255..3274
                     /label="pCEP_fwd_primer"
     misc_feature    3257..3281
                     /label="LNCX_primer"
     CDS             3364..4059
                     /label="ORF frame 1"
     misc_feature    4760..5323
                     /label="IRES"
     CDS             5336..5938
                     /label="ORF frame 2"
     gene            5351..5938
                     /label="puro (variant)"
                     /gene="puro (variant)"
     misc_feature    5956..6531
                     /label="WPRE"
     CDS             6044..6571
                     /label="ORF frame 2"
     misc_feature    6749..6801
                     /label="delta_U3"
     misc_feature    6802..6982
                     /label="HIV-1_5_LTR"
     misc_feature    6802..6982
                     /label="truncHIV-1_3_LTR"
     misc_feature    complement(7083..7100)
                     /label="BGH_rev_primer"
     terminator      7086..7313
                     /label="bGH_PA_terminator"
     rep_origin      7376..7682
                     /label="f1_origin"
     misc_feature    complement(7796..7816)
                     /label="pBABE_3_primer"
     misc_feature    complement(7802..8017)
                     /label="SV40_enhancer"
     promoter        7814..8082
                     /label="SV40_promoter"
     rep_origin      7981..8058
                     /label="SV40_origin"
     misc_feature    8043..8062
                     /label="SV40pro_F_primer"
     gene            8194..9205
                     /label="hygroB"
                     /gene="hygroB"
     CDS             complement(8288..8941)
                     /label="ORF frame 1"
     CDS             8537..9208
                     /label="ORF frame 2"
     terminator      9341..9460
                     /label="SV40_PA_terminator"
     misc_feature    9429..9448
                     /label="EBV_rev_primer"
     promoter        complement(9504..9522)
                     /label="M13_reverse_primer"
     misc_feature    complement(9521..9543)
                     /label="M13_pUC_rev_primer"
     promoter        complement(9557..9586)
                     /label="lac_promoter"
     gene            complement(10669..11529)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(10669..11529)
                     /label="ORF frame 2"
     promoter        complement(11571..11599)
                     /label="AmpR_promoter"
     terminator      11685..11816
                     /label="SV40_PA_terminator"
     misc_feature    11773..11792
                     /label="EBV_rev_primer"
ORIGIN
    1 Tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
   61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
  121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
  181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
  241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
  301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
  361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
  421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
  481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
  541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
  601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
  661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
  721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
  781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
  841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
  901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
  961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
 1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
 2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
 2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
 2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
 2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
 2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
 2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
 2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
 2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
 2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
 2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
 2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
 2701 cgaggttcta gagaccggtg gttaacgcgg atcccatcga ttgggagttc cgcgttacat
 2761 aacttaccgt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
 2821 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
 2881 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
 2941 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
 3001 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
 3061 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
 3121 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
 3181 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
 3241 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
 3301 gctgttttga cctccataga agacaccgac tctagctaga ggctagcgct accggtcgcc
 3361 accatgagcg agctgatcaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
 3421 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
 3481 accatgaaga tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
 3541 accagcttca tgtacggcag caaagccttc atcaaccaca cccagggcat ccccgacttc
 3601 tttaagcagt ccttccctga gggcttcaca tgggagagaa tcaccacata cgaagacggg
 3661 ggcgtgctga ccgctaccca ggacaccagc ttccagaacg gctgcatcat ctacaacgtc
 3721 aagatcaacg gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacacgcggc
 3781 tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctgagagg ccacagccag
 3841 atggccctga agctcgtggg cgggggctac ctgcactgct ccttcaagac cacatacaga
 3901 tccaagaaac ccgctaagaa cctcaagatg cccggcttcc acttcgtgga ccacagactg
 3961 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgagat ggctgtggcc
 4021 aagtactgcg acctccctag caaactgggg cacagataac tcgagcggac gcgtcggcgg
 4081 cctgtcggtc ccctctggct ctccgcgtgc gcccggggct gcgggagcgg agtccgctca
 4141 ggcccccgcg ccgggtcggc ggcggcacgt ggggcccaca gcgcggcagc ggtgccggcg
 4201 aatgggaggc ccgtttctct ctttggttat ctagctgtat gagtgccaca gagccgtcat
 4261 aaagctagat aaccgaaagt agaaatgact ctcacaactt ctgcgtgcga tggcccggcc
 4321 ggccgccctg gccacaggag ccagaggagc ccagagaagc ccacgccacc cagggattcc
 4381 agctcgacgg agcggccacg ccgggaccag gtatccgacc tcggctctcc ttctagtagg
 4441 tgggagtact gctcagagct acaactctag gagtagggac tccaagccta gagctccaag
 4501 agagggtgaa gggcagggag aaaattatag taatagttgc aatgagtcac ttgcttctag
 4561 atcaagatca gagactctgc tctccgtgtt cacagcggac cttgatttaa tgtcatacaa
 4621 ttaaggcacg cggtgaatgc caagagcgga gcctacagct gcacttgaag gacatccgag
 4681 agaagttagg aagggtgggg agaaacaatt ctagaatgaa cccatcctgt gcgacacggc
 4741 cgcaaattcc gcaaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc
 4801 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
 4861 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
 4921 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
 4981 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
 5041 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
 5101 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
 5161 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
 5221 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
 5281 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatggc
 5341 caccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
 5401 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
 5461 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
 5521 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
 5581 cgtcgaagcg ggggcggtgt tcgccgagat cggctcgcgc atggccgagt tgagcggttc
 5641 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
 5701 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
 5761 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgct ggggtgcccg ccttcctgga
 5821 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
 5881 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt
 5941 tcgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
 6001 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
 6061 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
 6121 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
 6181 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
 6241 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
 6301 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
 6361 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
 6421 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
 6481 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
 6541 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
 6601 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
 6661 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
 6721 agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa
 6781 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
 6841 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
 6901 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
 6961 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
 7021 cttgcaaaga aatgaatatc agagagtgag aggccttgac attgtttaaa cccgctgatc
 7081 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
 7141 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
 7201 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
 7261 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
 7321 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
 7381 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
 7441 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
 7501 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
 7561 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
 7621 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
 7681 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
 7741 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
 7801 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
 7861 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
 7921 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
 7981 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
 8041 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
 8101 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
 8161 ggatctgatc agcacgtgat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
 8221 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
 8281 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
 8341 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
 8401 ccggaagtgc ttgacattgg ggaattaatt cagcgagagc ctgacctatt gcatctcccg
 8461 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
 8521 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
 8581 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
 8641 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
 8701 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
 8761 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
 8821 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
 8881 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
 8941 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
 9001 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
 9061 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
 9121 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
 9181 cagcactcgt ccgagggcaa aggaatagca cgtgctacga gatttcgatt ccaccgccgc
 9241 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
 9301 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
 9361 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
 9421 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
 9481 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
 9541 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
 9601 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
 9661 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
 9721 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
 9781 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
 9841 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
 9901 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
 9961 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
10021 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
10081 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
10141 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
10201 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
10261 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
10321 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
10381 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
10441 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
10501 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
10561 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
10621 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
10681 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
10741 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
10801 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
10861 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
10921 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
10981 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
11041 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
11101 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
11161 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
11221 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
11281 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
11341 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
11401 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
11461 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
11521 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
11581 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
11641 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt
11701 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
11761 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
11821 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
11881 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
11941 aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//

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