| 质粒名称: | pAnc80L65AAP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 92307 |
| 存储实验室: | Luk Vandenberghe |
|
载体骨架基本信息 |
|
| 载体名称: | synthetic |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 5238 bp |
| 完整质粒大小: | 7446 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，AAV，合成生物学 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Ancestral AAV Capsid Anc80L65AAP,ANC80L65AAP |
| 物种来源: | Synthetic |
| 插入大小: | 2208 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | Rep2 |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | M13rev |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAnc80L65AAP was a gift from Luk Vandenberghe (Addgene plasmid # 92307 ; http://n2t.net/addgene:92307 ; RRID:Addgene_92307) |
| 参考文献部分: | A synthetic AAV vector enables safe and efficient gene transfer to the mammalian inner ear. Landegger LD, Pan B, Askew C, Wassmer SJ, Gluck SD, Galvin A, Taylor R, Forge A, Stankovic KM, Holt JR, Vandenberghe LH. Nat Biotechnol. 2017 Mar;35(3):280-284. doi: 10.1038/nbt.3781. Epub 2017 Feb 6. 10.1038/nbt.3781 PubMed 28165475 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAnc80L65AAP 7446 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep
expression construct with endogenous AAP.
ACCESSION .
VERSION .
KEYWORDS pAnc80L65AAP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7446)
AUTHORS Landegger LD, Pan B, Askew C, Wassmer SJ, Gluck SD, Galvin A, Taylor
R, Forge A, Stankovic KM, Holt JR, Vandenberghe LH
TITLE A synthetic AAV vector enables safe and efficient gene transfer to
the mammalian inner ear.
JOURNAL Nat Biotechnol. 2017 Mar;35(3):280-284. doi: 10.1038/nbt.3781. Epub
2017 Feb 6.
PUBMED 28165475
REFERENCE 2 (bases 1 to 7446)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7446
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(2891..2907)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2891..2907)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3052..3069)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3223..3811)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3303..3322)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
terminator 4042..4069
/label=T7Te terminator
/note="phage T7 early transcription terminator"
CDS complement(4088..4903)
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 4811..4830
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 4895..4914
/label=pENTR-R
/note="pENTR vectors, reverse primer"
terminator 5120..5147
/label=T7Te terminator
/note="phage T7 early transcription terminator"
rep_origin complement(5183..5638)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5320..5341)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 5532..5551
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5765..5787
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 5779..5796
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 5780..5796
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 5830..5846
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 5831..5847
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 gttgcgcagc catcgacgtc agacgcggaa gcttcgatca actacgcaga caggtaccaa
61 aacaaatgtt ctcgtcacgt gggcatgaat ctgatgctgt ttccctgcag acaatgcgag
121 agaatgaatc agaattcaaa tatctgcttc actcacggac agaaagactg tttagagtgc
181 tttcccgtgt cagaatctca acccgtttct gtcgtcaaaa aggcgtatca gaaactgtgc
241 tacattcatc atatcatggg aaaggtgcca gacgcttgca ctgcctgcga tctggtcaat
301 gtggatttgg atgactgcat ctttgaacaa taaatgattt aaatcaggta tggctgccga
361 tggttatctt ccagattggc tcgaggacaa cctctctgag ggcattcgcg agtggtggga
421 cttgaaacct ggagccccga aacccaaagc caaccagcaa aagcaggacg acggccgggg
481 tctggtgctt cctggctaca agtacctcgg acccttcaac ggactcgaca agggggagcc
541 cgtcaacgcg gcggacgcag cggccctcga gcacgacaag gcctacgacc agcagctcaa
601 agcgggtgac aatccgtacc tgcggtataa ccacgccgac gccgagtttc aggagcgtct
661 gcaagaagat acgtcttttg ggggcaacct cgggcgagca gtcttccagg ccaagaagcg
721 ggttctcgaa cctctcggtc tggttgagga aggcgctaag acggctcctg gaaagaagag
781 accggtagag caatcacccc aggaaccaga ctcctcttcg ggcatcggca agaaaggcca
841 gcagcccgcg agaaagagac tcaactttgg gcagactggc gactcagagt cagtgcccga
901 ccctcaacca ctcggagaac cccccgcagc cccctctggt gtgggatcta atacaatggc
961 tgcaggcggt ggcgctccaa tggcagacaa taacgaaggc gccgacggag tgggtaacgc
1021 ctcaggaaat tggcattgcg attccacatg gctgggcgac agagtcatca ccaccagcac
1081 ccgaacctgg gccctcccca cctacaacaa ccacctctac aagcaaatct ccagccaatc
1141 gggaggcagc accaacgaca acacctactt cggctacagc accccctggg ggtattttga
1201 ctttaacaga ttccactgcc acttctcacc acgtgactgg cagcgactca tcaacaacaa
1261 ctggggattc cggcccaaga agctcaactt caagctcttc aacatccagg tcaaggaggt
1321 cacgacgaat gatggcacca cgaccatcgc caataacctt accagcacgg ttcaggtctt
1381 tacggactcg gaataccagc tcccgtacgt cctcggctct gcgcaccagg gctgcctgcc
1441 tccgttcccg gcggacgtct tcatgattcc tcagtacggg tacctgactc tgaacaatgg
1501 cagtcaggcc gtgggccgtt cctccttcta ctgcctggaa tactttcctt ctcaaatgct
1561 gagaacgggc aacaactttc agttcagcta cacgtttgag gacgtgcctt ttcacagcag
1621 ctacgcgcac agccaaagcc tggaccggct gatgaacccc ctcatcgacc agtacctgta
1681 ctacctgtct cggactcaga ccacgagtgg taccgcagga aatcggacgt tgcaattttc
1741 tcaggccggg cctagtagca tggcgaatca ggccaaaaac tggctacccg ggccctgcta
1801 ccggcagcaa cgcgtctcca agacaaccaa tcaaaataac aacagcaact ttgcctggac
1861 cggtgccacc aagtatcatc tgaatggcag agactctctg gtaaatcccg gtcccgctat
1921 ggcaacccac aaggacgacg aagacaaatt ttttccgatg agcggagtct taatatttgg
1981 gaaacaggga gctggaaata gcaacgtgga ccttgacaac gttatgataa ccaacgagga
2041 agaaattaaa accaccaacc cagtggccac agaagagtac ggcacggtgg ccactaacct
2101 gcaatcggcc aacaccgctc ctgctacagg gaccgtcaac agtcaaggag ccttacctgg
2161 catggtctgg caggaccggg acgtgtacct gcagggtcct atctgggcca agattcctca
2221 cacggacgga cactttcatc cctcgccgct gatgggaggc tttggactga aacacccgcc
2281 tcctcagatc ctgattaaga atacacctgt tcccgcgaat cctccaacta ccttcagtcc
2341 agctaagttt gcgtcgttca tcacgcagta cagcaccgga caggtcagcg tggaaattga
2401 atgggagctg cagaaagaaa acagcaaacg ctggaaccca gagattcaat acacttccaa
2461 ctacaacaaa tctacaaatg tggactttgc tgttgacaca aatggcgttt attctgagcc
2521 tcgccccatc ggcacccgtt acctcacccg taatctgtaa actagtttgc ttgttaatca
2581 ataaaccgtt taattcgttt cagttgaact ttggtctctg cgtatttctt tcttatctag
2641 tttccatgct ctagaggtcc tgtattagag gtcacgtgag tgttttgcga cattttgcga
2701 caccatgtgg tcacgctggg tatttaagcc cgagtgagca cgcagggtct ccattttgaa
2761 gcgggaggtt tgaacgcgca gccgccaagc cgaattctgc agatatccat cacactggcg
2821 gccgctcgac tagagcggcc gccaccgcgg tggagctcca gcttttgttc gcgcgcttgg
2881 cgtaatcatg gtcatagctg tttcctgtgt gaaattccac agcctggggt gcctaattgc
2941 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
3001 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
3061 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
3121 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
3181 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
3241 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
3301 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
3361 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
3421 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
3481 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
3541 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
3601 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
3661 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
3721 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
3781 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
3841 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
3901 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
3961 tgagtaaact tggtctgaca gaaataataa aaaagccgga ttaataatct ggctttttat
4021 attctctctc tagtatataa acgcagaaag gcccacccga aggtgagcca gtgtgactct
4081 agtattatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
4141 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
4201 agttccaaag aatggcaagg tcctggtaac ggtctgcgat tccgacccgt ccaacatcaa
4261 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
4321 tgacgactga atccggtgag aatggcaaga gcttgtgcat ttctttccag acttgttcaa
4381 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcatgc
4441 gtgattgcgc ctgagcaaga cgaaatacac gatcgctgtt aaaaggacaa ttacaaacag
4501 gaatcgaatg taaccggcgc aggaacacgg ccagcgcatc aacaatattt tcacctgaat
4561 caggatattc ttctaatacc tggaaggctg ttttcccagg aatcgcggtg gtgagtaacc
4621 acgcatcatc aggagtacgg ataaaatgct tgatggtcgg gagaggcata aactccgtca
4681 gccagttgag acggaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
4741 tcagaaacaa ctctggcgca tcgggcttcc catacaagcg atagattgtc gcacctgatt
4801 gcccgacatt atcgcgagcc catttatacc catataaatc agcgtccatg ttggagttta
4861 agcgcggacg ggagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
4921 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
4981 aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt
5041 tgaaggatca gctctagtaa aataataaaa aagccggatt aataatctgg ctttttatat
5101 tctctctcta gtatataaac gcagaaaggc ccacccgaag gtgagccagt gtgacggcac
5161 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
5221 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
5281 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
5341 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
5401 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
5461 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
5521 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
5581 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
5641 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
5701 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
5761 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcggcgaa ttgggtaccg
5821 ggccccccct cgaggtcgac ggtatcgtat ttaagcccga gtgagcacgc agggtctcca
5881 ttttgaagcg ggaggtttga acgcgcagcc gccatgccgg ggttttacga gattgtgatt
5941 aaggtcccca gcgaccttga cgagcatctg cccggcattt ctgacagctt tgtgaactgg
6001 gtggccgaga aggaatggga gttgccgcca gattctgaca tggatctgaa tctgattgag
6061 caggcacccc tgaccgtggc cgagaagctg cagcgcgact ttctgacgga atggcgccgt
6121 gtgagtaagg ccccggaggc tcttttcttt gtgcaatttg agaagggaga gagctacttc
6181 cacatgcacg tgctcgtgga aaccaccggg gtgaaatcca tggttttggg acgtttcctg
6241 agtcagattc gcgaaaaact gattcagaga atttaccgcg ggatcgagcc gactttgcca
6301 aactggttcg cggtcacaaa gaccagaaat ggcgccggag gcgggaacaa ggtggtggat
6361 gagtgctaca tccccaatta cttgctcccc aaaacccagc ctgagctcca gtgggcgtgg
6421 actaatatgg aacagtattt aagcgcctgt ttgaatctca cggagcgtaa acggttggtg
6481 gcgcagcatc tgacgcacgt gtcgcagacg caggagcaga acaaagagaa tcagaatccc
6541 aattctgatg cgccggtgat cagatcaaaa acttcagcca ggtacatgga gctggtcggg
6601 tggctcgtgg acaaggggat tacctcggag aagcagtgga tccaggagga ccaggcctca
6661 tacatctcct tcaatgcggc ctccaactcg cggtcccaaa tcaaggctgc cttggacaat
6721 gcgggaaaga ttatgagcct gactaaaacc gcccccgact acctggtggg ccagcagccc
6781 gtggaggaca tttccagcaa tcggatttat aaaattttgg aactaaacgg gtacgatccc
6841 caatatgcgg cttccgtctt tctgggatgg gccacgaaaa agttcggcaa gaggaacacc
6901 atctggctgt ttgggcctgc aactaccggg aagaccaaca tcgcggaggc catagcccac
6961 actgtgccct tctacgggtg cgtaaactgg accaatgaga actttccctt caacgactgt
7021 gtcgacaaga tggtgatctg gtgggaggag gggaagatga ccgccaaggt cgtggagtcg
7081 gccaaagcca ttctcggagg aagcaaggtg cgcgtggacc agaaatgcaa gtcctcggcc
7141 cagatagacc cgactcccgt gatcgtcacc tccaacacca acatgtgcgc cgtgattgac
7201 gggaactcaa cgaccttcga acaccagcag ccgttgcaag accggatgtt caaatttgaa
7261 ctcacccgcc gtctggatca tgactttggg aaggtcacca agcaggaagt caaagacttt
7321 ttccggtggg caaaggatca cgtggttgag gtggagcatg aattctacgt caaaaagggt
7381 ggagccaaga aaagacccgc ccccagtgac gcagatataa gtgagcccaa acgggtgcgc
7441 gagtca
//
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