| 出品公司: | 爱迪基因 |
|---|---|
| 载体名称: | pCS2+8CmCherry |
| 质粒类型: | 克隆载体；哺乳动物表达载体；广宿主载体；体外表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | simian CMV IE94、lac、T3、SP6 |
| 载体大小: | 4848 bp |
| 5' 测序引物及序列: | SP6 fwd（MCS1）、T3 fwd（MCS2） |
| 3' 测序引物及序列: | EBV rev（MCS1）、SV40 polyA rev（MCS2） |
| 载体标签: | mCherry |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH10B |
| 表达细胞: | 原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。 |
| 备注: | pCS2+8CmCherry载体能够在多种细胞中进行高水平的瞬表达。 pCS2+8CmCherry载体同时也能进行体外转录和翻译。 |
| 产品目录号: | #34935 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pCS2+8CmCherry载体质粒基本信息
pCS2+8CmCherry质粒图谱载体图谱和pCS2+8CmCherry载体序列质粒序列多克隆位点信息
pCS2+8CmCherry质粒载体简介
pCS2+8CmCherry质粒序列载体序列
LOCUS pCS2+8CmCherry Sequencing Result 4848 bp DNA SYN
DEFINITION pCS2+8CmCherry Sequencing Result
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pCS2+8CmCherry|
FEATURES Location/Qualifiers
source 1..4848
/organism="pCS2+8CmCherry Sequencing Result"
/mol_type="other DNA"
misc_feature 186..208
/label="M13_pUC_rev_primer"
promoter 207..225
/label="M13_reverse_primer"
promoter 242..261
/label="T3_promoter"
misc_feature 464..483
/label="EBV_rev_primer"
CDS complement(558..1268)
/label="ORF frame 2"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
YERAEGRHSTGGMDELYK*"
gene complement(561..1265)
/label="mCherry"
/gene="mCherry"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
YERAEGRHSTGGMDELYK*"
misc_feature complement(1035..1088)
/label="Q66M (mCherry)"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
YERAEGRHSTGGMDELYK*"
misc_feature complement(1355..1372)
/label="Sp6_primer"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
YERAEGRHSTGGMDELYK*"
misc_feature complement(1448..1489)
/label="CMV_fwd_primer"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
YERAEGRHSTGGMDELYK*"
CDS complement(1958..2482)
/label="ORF frame 3"
/translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP
IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH
GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA
LCHIVFHIWVFLLT*"
rep_origin 2479..2785
/label="f1_origin"
/translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP
IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH
GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA
LCHIVFHIWVFLLT*"
promoter 2978..3006
/label="AmpR_promoter"
/translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP
IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH
GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA
LCHIVFHIWVFLLT*"
gene 3048..3908
/label="Ampicillin"
/gene="Ampicillin"
/translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP
IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH
GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA
LCHIVFHIWVFLLT*"
CDS 3048..3908
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 4063..4682
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgCAGCTGGC
61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC
121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTCCC GGCTCGTATG TTGTGTGGAA
181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCGCG
241 CAATTAACCC TCACTAAAGG GAACAAAAGC TGGGTACCGG GCCCAATGCA TTGGCGCCGC
301 GGCCGCGAAT TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA
361 ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC
421 ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC
481 ATCAATGTAT CTTATCATGT CTGGATCTAC GTAATACGAC TCACTATAGT TCTAGAGGCT
541 CGAGAGGCCT TGAATTCTTA CTTGTACAGC TCGTCCATGC CGCCGGTGGA GTGGCGGCCC
601 TCGGCGCGTT CGTACTGTTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT GATGTCCAAC
661 TTGATGTTGA CGTTGTAGGC GCCGGGCAGC TGCACGGGCT TCTTGGCCTT GTAGGTGGTC
721 TTGACCTCAG CGTCGTAGTG GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT GATCTCGCCC
781 TTCAGGGCGC CGTCCTCGGG GTACATCCGC TCGGAGGAGG CCTCCCAGCC CATGGTCTTC
841 TTCTGCATTA CGGGGCCGTC GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC CTTGTAGATG
901 AACTCGCCGT CCTGCAGGGA GGAGTCCTGG GTCACGGTCA CCACGCCGCC GTCCTCGAAG
961 TTCATCACGC GCTCCCACTT GAAGCCCTCG GGGAAGGACA GCTTCAAGTA GTCGGGGATG
1021 TCGGCGGGGT GCTTCACGTA GGCCTTGGAG CCGTACATGA ACTGAGGGGA CAGGATGTCC
1081 CAGGCGAAGG GCAGGGGGCC ACCCTTGGTC ACCTTCAGCT TGGCGGTCTG GGTGCCCTCG
1141 TAGGGGCGGC CCTCGCCCTC GCCCTCGATC TCGAACTCGT GGCCGTTCAC GGAGCCCTCC
1201 ATGTGCACCT TGAAGCGCAT GAACTCCTTG ATGATGGCCA TGTTATCCTC CTCGCCCTTG
1261 CTCACCATCA ATCGATGTCC GGCGATCGCT GGCCGGCCTT TAATTAATGG CGCGCCACTA
1321 GTGGATCCTG CAAAAAGAAC AAGTAGCTTG TATTCTATAG TGTCACCTAA ATCAAGCTTG
1381 CTCCGACGTC CCCAGGCAGA ATGGCGGTTC CCTAAACGAG CATTGCTTAT ATAGACCTCC
1441 CATTAGGCAC GCCTACCGCC CATTTACGTC AATGGAACGC CCATTTGCGT CATTGCCCCT
1501 CCCCATTGAC GTCAATGGGG ATGTACTTGG CAGCCATCGC GGGCCATTTA CCGCCATTGA
1561 CGTCAATGGG AGTACTGCCA ATGTACCCTG GCGTACTTCC AATAGTAATG TACTTGCCAA
1621 GTTACTATTA ATAGATATTG ATGTACTGCC AAGTGGGCCA TTTACCGTCA TTGACGTCAA
1681 TAGGGGGCGT GAGAACGGAT ATGAATGGGC AATGAGCCAT CCCATTGACG TCAATGGTGG
1741 GTGGTCCTAT TGACGTCAAT GGGCATTGAG CCAGGCGGGC CATTTACCGT AATTGACGTC
1801 AATGGGGGAG GCGCCATATA CGTCAATAGG ACCGCCCATA TGACGTCAAT AGGAAAGACC
1861 ATATATAGAG ACCATTGACG TCAATGGGGG AGTGGCTATG GGCGGTATTA GGAAGCCCCA
1921 TATATGGTAT ATGGGACCGC CCATTGGGAG GGGCTATCTA CGTCAATAGG AAAACCCATA
1981 TATGGAATAC TATATGGCAT AGGGCCAATA CATAGTATTG AACCTGGCCA ATAGCCATAT
2041 TGGCATAGGG CCATATTGGA TATTGCCTAT ATATTGATCC TGGCATATAG CCAATATGGC
2101 CGCCATTATT GGCACCATGC CAATTCAATA TGGCGGACTT GGCACAGGGC CAAGACCCCT
2161 CCTCAAACTT GGCACCGTGC CAAGTAGACC CCTCCCCAGT TGGCACAGTG CCAGGTCCGC
2221 CATATTGAAT TGGCATGGTG CCAAGTCCGC CATATTGAAT TGGCTGGGGT CCAGATCCAC
2281 CATATTGAAT TGGCACGAGT CCATATACGC CATATTGAAT TGGCACAGGT CCAGATCCAC
2341 CATATTGAAT TGGCATGAGT CCATATACGC CATATTGAAT TGGCGGTCGA CTGGCGTAAT
2401 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG
2461 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC
2521 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC
2581 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT
2641 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG
2701 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT
2761 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA
2821 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT
2881 AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTAGG TGGCACTTTT CGGGGAAATG
2941 TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA
3001 GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC
3061 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC
3121 CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA
3181 TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC
3241 CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG
3301 GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC
3361 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA
3421 TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG
3481 AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC
3541 CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG
3601 CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT
3661 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG
3721 CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG
3781 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC
3841 AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC
3901 ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT
3961 TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT
4021 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT
4081 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG
4141 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA
4201 GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA
4261 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG
4321 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG
4381 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT
4441 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA
4501 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC
4561 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG
4621 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG
4681 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT
4741 TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC
4801 GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAG
//
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pCS2+8CmCherry质粒载体应用举例
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