| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-Het-2 |
| 质粒类型: | 慢病毒载体；双顺反子载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 8902 bp |
| 5' 测序引物及序列: | CMV Forward： CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | DmrA |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 潮霉素（Hygromycin） |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | 慢病毒载体pLVX-Het-2表达DmrA融合蛋白；两个MCS区。 |
| 产品目录号: | 635075 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-Het2 8902 bp DNA circular 8-NOV-2011
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Cat. No.635074, 635075
FEATURES Location/Qualifiers
LTR 1..635
/label=5'\LTR
misc_feature 685..822
/label=packaging\signal
misc_feature 2028..2151
/label=CTS/cppt\(DNA\flap)
promoter 2185..2788
/label=pCMV
misc_feature 4824..5415
/label=WPRE
rep_origin complement(6725..7395)
/label=pUC\origin
CDS complement(7540..8536)
/label=Amp(R)
misc_binding 1303..1536
/label=RRE
LTR 5619..6255
/label=LTR
CDS 3776..4810
/label=Hyg(R)
misc_feature 3177..3752
/label=IRES
misc_feature 2822..3151
/label=DmrA
BASE COUNT 2294 a 2156 c 2299 g 2153 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tagctagagg atctatttcc ggtgaattcc tcgagactag
2821 tatgtctaga ggagtccagg tggaaaccat ctccccagga gacgggcgca ccttccccaa
2881 gcgcggccag acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga
2941 ttcctcccgg gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg
3001 aggctgggaa gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc
3061 tccagattat gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct
3121 cgtcttcgat gtggagcttc taaaactgga aagcggccgc ggatcctacg tatgaccgcc
3181 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
3241 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
3301 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
3361 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
3421 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
3481 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
3541 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
3601 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
3661 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
3721 acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccgtac caaagatgga
3781 tagatccgga aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
3841 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
3901 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
3961 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
4021 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
4081 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
4141 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
4201 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
4261 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
4321 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
4381 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
4441 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
4501 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
4561 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
4621 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
4681 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
4741 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
4801 aaaggaatag acgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga
4861 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
4921 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
4981 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
5041 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
5101 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
5161 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
5221 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
5281 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
5341 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
5401 ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac
5461 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
5521 ggaggtgggt ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
5581 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
5641 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
5701 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
5761 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
5821 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
5881 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
5941 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
6001 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
6061 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
6121 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
6181 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
6241 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
6301 aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt cgacctctag
6361 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
6421 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
6481 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
6541 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
6601 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
6661 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
6721 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
6781 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
6841 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
6901 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
6961 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
7021 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
7081 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
7141 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
7201 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
7261 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
7321 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
7381 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
7441 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
7501 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
7561 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
7621 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
7681 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
7741 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
7801 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
7861 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
7921 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
7981 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
8041 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
8101 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
8161 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
8221 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
8281 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
8341 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
8401 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
8461 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
8521 agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt
8581 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
8641 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
8701 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
8761 tagtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
8821 tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt atttgttaaa
8881 tatgtactac aaacttagta gt
//
