| 出品公司: | BD Biosciences |
|---|---|
| 载体名称: | pAcSG2 |
| 质粒类型: | 昆虫细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 5544 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAcSG2 5544 bp ds-DNA circular SYN 19-APR-2014
DEFINITION Compact baculovirus transfer vector for expressing proteins using
the polyhedrin promoter, for use with the BD BaculoGold(TM) system.
ACCESSION .
VERSION .
KEYWORDS pAcSG2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5544)
AUTHORS BD Biosciences
TITLE Direct Submission
JOURNAL Exported Thursday, February 6, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
COMMENT This vector accepts inserts as large as 8 kb.
FEATURES Location/Qualifiers
source 1..5544
/organism="synthetic DNA construct"
/lab_host="Insect Cells"
/mol_type="other DNA"
misc_recomb 1..588
/note="baculovirus recombination region (ORF603)"
/note="contains ORF603 and part of lef2"
CDS complement(1..528)
/codon_start=1
/product="baculovirus ORF603 protein"
/note="ORF603"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
INCESFENDFKYY"
promoter 592..683
/gene="polh from Autographa californica"
/note="polyhedrin promoter"
/note="promoter for the baculovirus polyhedrin gene"
misc_feature 690..751
/note="MCS"
/note="multiple cloning site"
CDS 710..712
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
misc_recomb 771..1476
/note="baculovirus recombination region (ORF1629)"
/note="contains part of ORF1629"
CDS complement(888..1476)
/codon_start=2
/product="baculovirus capsid-associated protein"
/note="ORF1629"
/note="required for viral replication"
/translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
rep_origin complement(3539..4127)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4298..5158)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5159..5263)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
61 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
121 ctcctcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
181 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
241 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
301 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
361 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
421 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
481 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
541 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tatcatggag
601 ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt
661 ttgtaataaa aaaacctata aatacggatc tcgaggaatt caggcctcca tgggagctcg
721 cggccgcctg cagggtaccc ccgggagatc tgtaccgact ctgctgaaga ggaggaaatt
781 ctccttgaag tttccctggt gttcaaagta aaggagtttg caccagacgc acctctgttc
841 actggtccgg cgtattaaaa cacgatacat tgttattagt acatttatta agcgctagat
901 tctgtgcgtt gttgatttac agacaattgt tgtacgtatt ttaataattc attaaattta
961 taatctttag ggtggtatgt tagagcgaaa atcaaatgat tttcagcgtc tttatatctg
1021 aatttaaata ttaaatcctc aatagatttg taaaataggt ttcgattagt ttcaaacaag
1081 ggttgttttt ccgaaccgat ggctggacta tctaatggat tttcgctcaa cgccacaaaa
1141 cttgccaaat cttgtagcag caatctagct ttgtcgatat tcgtttgtgt tttgttttgt
1201 aataaaggtt cgacgtcgtt caaaatatta tgcgcttttg tatttctttc atcactgtcg
1261 ttagtgtaca attgactcga cgtaaacacg ttaaataaag cttggacata tttaacatcg
1321 ggcgtgttag ctttattagg ccgattatcg tcgtcgtccc aaccctcgtc gttagaagtt
1381 gcttccgaag acgattttgc catagccaca cgacgcctat taattgtgtc ggctaacacg
1441 tccgcgatca aatttgtagt tgagcttttt ggaattattt ctgattgcgg gcgtttttgg
1501 gcgggtttca atctaactgt gcccgatttt aattcagaca acacgttaga aagcgatggt
1561 gcaggcggtg gtaacatttc agacggcaaa tctactaatg gcggcggtgg tggagctgat
1621 gataaatcta ccatcggtgg aggcgcaggc ggggctggcg gcggaggcgg aggcggaggt
1681 ggtggcggtg atgcagacgg cggtttaggc tcaaatgtct ctttaggcaa cacagtcggc
1741 acctcaacta ttgtactggt ttcgggcgcc gtttttggtt tgaccggtct gagacgagtg
1801 cgattttttt cgtttctaat agcttccaac aattgttgtc tgtcgtctaa aggtgcagcg
1861 ggttgaggtt ccgtcggcat tggtggagcg ggcggcaatt cagacatcga tggtggtggt
1921 ggtggtggag gcgctggaat gttaggcacg ggagaaggtg gtggcggcgg tgccgccggt
1981 ataatttgtt ctggtttagt ttgttcgcgc acgattgtgg gcaccggcgc aggcgccgct
2041 ggctgcacaa cggaaggtcg tctgcttcga ggcagcgctt ggggtggtgg caattcaata
2101 ttataattgg aatacaaatc gtaaaaatct gctataagca ttgtaatttc gctatcgttt
2161 accgtgccga tatttaacaa ccgctcaatg taagcaattg tattgtaaag agattgtctc
2221 aagctccgca cgccgataac aagccttttc atttttacta cagcattgta gtggcgagac
2281 acttcgctgt cgtcgacgta catgtatgct ttgttgtcaa aaacgtcgtt ggcaagcttt
2341 aaaatattta aaagaacatc tctgttcagc accactgtgt tgtcgtaaat gttgtttttg
2401 ataatttgcg cttccgcagt atcgacacgt tcaaaaaatt gatgcgcatc aattttgttg
2461 ttcctattat tgaataaata agattgtaca gattcatatc tacgattcgt catggccacc
2521 acaaatgcta cgctgcaaac gctggtacaa ttttacgaaa actgcaaaaa cgtcaaaact
2581 cggtataaaa taatcaacgg gcgctttggc aaaatatcta ttttatcgca caagcccact
2641 agcaaattgt atttgcagaa aacaatttcg gcgcacaatt ttaacgctga cgaaataaaa
2701 gttcaccagt taatgagcga ccacccaaat tttataaaaa tctattttaa tcacggttcc
2761 atcaacaacc aagtgatcgt gatggactac attgactgtc ccgatttatt tgaaacacta
2821 caaattaaag gcgagctttc gtaccaactt gttagcaata ttattagaca gctgtgtgaa
2881 gcgctcaacg atttgcacaa gcacaatttc atacacaacg acataaaact cgaaaatgtc
2941 ttatatttcg aagcacttga tcgcgtgtat gtttgcgatt acggattgtg caaacacgaa
3001 aactcactta gcgtgcacga cggcacgttg gagtatttta gtccggaaaa aattcgacac
3061 acaactatgc acgtttcgtt tgactggtac gcggcgtgtt aacatacaag ttgctaaccg
3121 gcggttcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
3181 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
3241 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
3301 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
3361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
3421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
3481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
3541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
3601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
3661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
3721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
3781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
3841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
3901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
3961 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
4021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
4081 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
4141 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
4201 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
4261 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
4321 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
4381 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
4441 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
4501 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
4561 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
4621 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
4681 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
4741 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
4801 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
4861 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
4921 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
4981 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
5041 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
5101 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
5161 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
5221 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
5281 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
5341 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
5401 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
5461 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
5521 agattgtact gagagtgcac cata
//
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