出品人: | Irene Castano Navarro(2015) |
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载体名称: | pYC121 |
质粒类型: | 酿酒酵母载体;复制型载体;过表达载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | TEF1 |
载体大小: | 6277 bp |
5' 测序引物及序列: | M13/pUC fwd primer 5’ CCAGTCACGACGTTGTAAAACG 3’ |
3' 测序引物及序列: | -- |
载体标签: | Myc tag |
载体抗性: | 氨苄青霉素 |
筛选标记: | URA3 |
克隆菌株: | DH10B感受态细胞 ,30°C |
宿主细胞(系): | 出芽酵母(酿酒酵母)、白色念珠菌 |
备注: | -- |
产品目录号: | 63917 |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pYC121
价格:1200元
联系方式:I47-825O-882O
买家导航
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pYC121 6277 bp DNA SYN DEFINITION pYC121 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..6277 /organism="pYC121" /mol_type="other DNA" promoter 668..696 /label="AmpR_promoter" gene 738..1598 /label="Ampicillin" /gene="Ampicillin" CDS 738..1598 /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" rep_origin 1753..2372 /label="pBR322_origin" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 2724..2746 /label="M13_pUC_rev_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter 2745..2763 /label="M13_reverse_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter 2780..2799 /label="T3_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 3218..3234 /label="pBluescriptSK_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature complement(3853..3869) /label="pBluescriptKS_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" CDS 4105..4689 /label="ORF frame 1" /translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" promoter complement(4283..4301) /label="T7_promoter" /translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" promoter complement(4311..4327) /label="M13_forward20_primer" /translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" misc_feature complement(4320..4342) /label="M13_pUC_fwd_primer" /translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" CDS complement(5058..5861) /label="ORF frame 3" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*" gene complement(5061..5861) /label="URA3" /gene="URA3" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*" CDS 5350..5826 /label="ORF frame 1" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttaGCAGGT TAGACTAACA TGCAAAAAGT TTATATTCAT TTTTGTAGTT ATTCTTGTTT 121 TCATTAGGAT GATTACACAC TGTGGATGTG GATTAATCGA TCATTGTTAA TTTAGTGTAG 181 CTACTACCAT TTCAAACAAA ACGTAAATTC TTGCAGTCGT ACTGGATCTG TGAATCTATT 241 AGTATATATG AATTAAAGTA GCTTGTACAT ATTATTCTGT TGAATCATAT CGCAGAGCAT 301 TGGTTGAAAT CCAAAATATA AAAATGTAAT ATCACAAAAA ATAATACTAA TTCTAACATT 361 AATGGGTCAG ATTTTTAGTG AATACTTAAA TTTATAATAT CGTCTATTTA AGCTAGCAAA 421 TGGAACAACA TTTAAAGTAA GAACATCATA TCTACATGAA AATGTATATT TCAATCTGAC 481 TAATAACGCA GAGCACATCT TTCAGTGATG TCTGTCACAT GATCAAAAAG AATTGTATTT 541 AAATAATTCA TAATAAAAGC TTAAAAAATT Acaataatga aaataaagtA AATAATGACA 601 TGGGTAAGAG TTCCGAAATA GAATCTTAGT GTACAAAACA AAAATTGCAT CATTAGAGAT 661 CCCCacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 721 attgaaaaag gaagagtATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG 781 CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG 841 AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC 901 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT 961 GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT 1021 ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA 1081 TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT 1141 TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG 1201 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG 1261 AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG 1321 AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG 1381 CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG 1441 CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC 1501 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA 1561 TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAAct gtcagaccaa gtttactcat 1621 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 1681 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 1741 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 1801 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 1861 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc 1921 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 1981 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 2041 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 2101 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 2161 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 2221 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 2281 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 2341 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 2401 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 2461 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 2521 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 2581 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 2641 caattaatgt gagttacctc actcattagg caccccaggc tttacacttt atgcttccgg 2701 ctcctatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc 2761 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gAGCTCATAG 2821 CTTCAAAATG TTTCTACTCC TTTTTTACTC TTCCAGATTT TCTCGGACTC CGCGCATCGC 2881 CGTACCACTT CAAAACACCC AAGCACAGCA TACTAAATTT CCCCTCTTTC TTCCTCTAGG 2941 GTGTCGTTAA TTACCCGTAC TAAAGGTTTG GAAAAGAAAA AAGAGACCGC CTCGTTTCTT 3001 TTTCTTCGTC GAAAAAGGCA ATAAAAATTT TTATCACGTT TCTTTTTCTT GAAAATTTTT 3061 TTTTTGATTT TTTTCTCTTT CGATGACCTC CCATTGATAT TTAAGTTAAT AAACGGTCTT 3121 CAATTTCTCA AGTTTCAGTT TCATTTTTCT TGTTCTATTA CAACTTTTTT TACTTCTTGC 3181 TCATTAGAAA GAAAGCATAG CAATCTAATC TAAGTTTTCT AGaactagtg gatcctaggg 3241 TTAATTAACG GTGAACAAAA GCTAATCTCC GAGGAAGACT TGAACGGTGA ACAAAAATTA 3301 ATCTCAGAAG AAGACTTGAA CGGACTCGAC GGTGAACAAA AGTTGATTTC TGAAGAAGAT 3361 TTGAACGGTG AACAAAAGCT AATCTCCGAG GAAGACTTGA ACGGTGAACA AAAATTAATC 3421 TCAGAAGAAG ACTTGAACGG ACTCGACGGT GAACAAAAGT TGATTTCTGA AGAAGATTTG 3481 AACGGTGAAC AAAAGCTAAT CTCCGAGGAA GACTTGAACG GTGAACAAAA ATTAATCTCA 3541 GAAGAAGACT TGAACGGACT CGACGGTGAA CAAAAGTTGA TTTCTGAAGA AGATTTGAAC 3601 GGTGAACAAA AGCTAATCTC CGAGGAAGAC TTGAACGGTG AACAAAAATT AATCTCAGAA 3661 GAAGACTTGA ACGGACTCGA CGGTGAACAA AAGTTGATTT CTGAAGAAGA TTTGAACGGT 3721 GAACAAAAGC TAATCTCCGA GGAAGACTTG AACGGTGAAC AAAAATTAAT CAATCACtag 3781 ctagtgaatt cgcgccagat ccGAAGTTCC TATACTTTct aggggctgca ggaattcgat 3841 atcaagctta tcgataccgt cgacCTCGAG AACACAACCC ACAGCTACCA CCATCAACAA 3901 TATTTATATA TATAACGTAC ACATAGAAAT CACACAAACA GAGTATTTAT TCTTAACTAC 3961 ATGAACTACC ATCAGACCGT CTGGGCCACT ATATAATGTG CCATTCATAA ACGTGATCAC 4021 TTTACGTAGC AGGCAACCCC AGGTGAAAAT TTTTCAGCGA GCTGCCAGAT TGTCAGGTGA 4081 AAAACTGAAA AAAACTTCTG GGCGATGAGC TTGTGGCGGG AAATTAAGTA TATAAAGCAG 4141 TTAGTTTCTT CTGCTTCTTG TGGTTCTGGG TATTCTTGTA ATATTTGGAA GAATAGAAAA 4201 TCAAGACTAC TGCCTTTCTT TTCATATTAT AGAGGAAGAA ATACGCACGA ACACGATATA 4261 GAGGTAAAGG TACCcaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc 4321 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 4381 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 4441 cagttgcgca gcctgaatgg cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg 4501 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 4561 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 4621 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 4681 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 4741 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 4801 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 4861 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt 4921 acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag 4981 ggtaataact gatataatta aattgaagct ctaatttgtg agtttagtat acatgcattt 5041 acttataata cagttttTTA GTTTTGCTGG CCGCATCTTC TCAAATATGC TTCCCAGCCT 5101 GCTTTTCTGT AACGTTCACC CTCTACCTTA GCATCCCTTC CCTTTGCAAA TAGTCCTCTT 5161 CCAACAATAA TAATGTCAGA TCCTGTAGAG ACCACATCAT CCACGGTTCT ATACTGTTGA 5221 CCCAATGCGT CTCCCTTGTC ATCTAAACCC ACACCGGGTG TCATAATCAA CCAATCGTAA 5281 CCTTCATCTC TTCCACCCAT GTCTCTTTGA GCAATAAAGC CGATAACAAA ATCTTTGTCG 5341 CTCTTCGCAA TGTCAACAGT ACCCTTAGTA TATTCTCCAG TAGATAGGGA GCCCTTGCAT 5401 GACAATTCTG CTAACATCAA AAGGCCTCTA GGTTCCTTTG TTACTTCTTC TGCCGCCTGC 5461 TTCAAACCGC TAACAATACC TGGGCCCACC ACACCGTGTG CATTCGTAAT GTCTGCCCAT 5521 TCTGCTATTC TGTATACACC CGCAGAGTAC TGCAATTTGA CTGTATTACC AATGTCAGCA 5581 AATTTTCTGT CTTCGAAGAG TAAAAAATTG TACTTGGCGG ATAATGCCTT TAGCGGCTTA 5641 ACTGTGCCCT CCATGGAAAA ATCAGTCAAG ATATCCACAT GTGTTTTTAG TAAACAAATT 5701 TTGGGACCTA ATGCTTCAAC TAACTCCAGT AATTCCTTGG TGGTACGAAC ATCCAATGAA 5761 GCACACAAGT TTGTTTGCTT TTCGTGCATG ATATTAAATA GCTTGGCAGC AACAGGACTA 5821 GGATGAGTAG CAGCACGTTC CTTATATGTA GCTTTCGACA Tgatttatct tcgtttcctg 5881 caggtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt ttcttcaaca 5941 ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc ttccttctgt 6001 tcggagatta ccgaatcaaa aaaatttcaa agaaaccgaa atcaaaaaaa agaataaaaa 6061 aaaaatgatg aattgaattg aaaagctgtg gtatggtgca ctctcagtac aatctgctct 6121 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg 6181 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagGtgcatg 6241 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcga //