质粒名称: | pBbdCas9S_arr2 |
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出品公司: | Addgene |
目录编号: | 149639 |
存储实验室: | Christine Jacobs-Wagner |
载体骨架基本信息 |
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载体名称: | pBbdCas9S |
载体出品公司: | CJW lab |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | dCas9, lacI, arr2 |
载体类型: | 细菌表达,CRISPR |
筛选标记: | 利福平 |
载体抗性: | 大观霉素 |
生长温度: | 30 ℃ |
宿主菌: | NEB 5-alpha F' Iq |
拷贝数: | 高拷贝 |
插入基因信息 |
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插入基因名称: | -- |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子: | PflaB, PpQE30 |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5’克隆位点: | -- |
3’克隆位点: | -- |
5’测序引物 | -- |
3’测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pBbdCas9S_arr2 was a gift from Christine Jacobs-Wagner (Addgene plasmid # 149639 ; http://n2t.net/addgene:149639 ; RRID:Addgene_149639) |
参考文献部分: | A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi. Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C. Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 10.1128/AEM.02519-20. 10.1128/AEM.02519-20 PubMed 33257311 |
买家导航
pBbdCas9Sarr2载体基本信息
pBbdCas9Sarr2载体图谱质粒图谱和多克隆位点信息
pBbdCas9Sarr2载体简介
pBbdCas9Sarr2载体序列
LOCUS pBbdCas9S_arr2 11315 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning. ACCESSION . VERSION . KEYWORDS pBbdCas9S_arr2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11315) AUTHORS Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C TITLE A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi. JOURNAL Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 10.1128/AEM.02519-20. PUBMED 33257311 REFERENCE 2 (bases 1 to 11315) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11315 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1603..1647 /label=T5 promoter /note="bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator" protein_bind 1623..1639 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 1655..1671 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 1690..1701 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS 1708..5814 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 5984..6775 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 6898..7486 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7387..7406 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 aaaaatttcc tggtaatcca acacaatctt atagaacttg tgaaccttta agaattgttg 61 gtgttgttga agattgggaa ggacatcctg ttgaattaat aagaggaatg ttagattcat 121 tagaagattt aaaacgtcgt ggtttacatg ttattgaaga ttaacctggc gcgccaaata 181 atgcccgtgt ccctgttgaa tagggacacg gttttttaac aattttttta ttcctaggcc 241 ttagtggccg gtacccgggg atccttatta ctggccgctt tctagcctgg atacttgtct 301 ggctagttgc attagagagt cagctagagc cctagggcta gctgtttgtg tattcggagc 361 cagtgtagtt tttcttttta ctaacgatac gggtaataat tgattgcctt ttacagcctg 421 accttgagac aattgcaaaa gtctgtccac agaggtttga cctaataatc taaaatcttg 481 tttgatagta gtaagtggag gtatgtagca actagaatct tctgtatcat cataacccac 541 aactgaaata tcagccccaa ctctaagtcc actttcagta atagctcgca tagctcctaa 601 agccatttga tcattagcaa ctaacatagc agtagggaca atgccttcat ttaacatttg 661 cattgtttgc tgaaatcctg acatagcaga ccaatctcct tctctttctg ctattggttg 721 tatttgattt cttgttaaat acttgtgcca tcctgctaat ctcaatctgg cacttactga 781 acttaaggga cctgcaagta gtgcaatttg ttgatggcct aatgcaacaa ggtgttcaac 841 tcctaacctt gttccatctt cgtgagaaaa aattatagag tttattgggg tttggtccga 901 cacatccaaa aacaatgctg gaacattagt acatgctgct tcaactgcaa tcgcatcttg 961 gtcatcaaga ggataattaa ttataagtcc actaacacgt tgagctaata aattatgtac 1021 tgctgcctta caagcctcta cacctgatct ctcaaccatt gatacaacaa cgcttgcacc 1081 aagttgatca gctctcgatt taattgcggc tacaatttga ctaggcgcat gtaatgcaag 1141 tgatgatgtg gcaactccaa ttaaaagtga ttgtttcccc gcaagttgct gagcaacgcg 1201 atttggtata taattcaact ctgccatagc tgcttcaacc ttctctcttg tttttgcaga 1261 tacatgactt gcttggttca caactctcga aaccgtttgg tagctaaccc cggcatattc 1321 agcaacatca tacaaagtaa caggtttcat ggatcattcc tccatgataa aatttaaatt 1381 tctgactttg gcaaatcctt ttgcctgtta aaaagaatag cacaaaaatt aaaaaaaaag 1441 aaacgcaaat agcaatagca ttaaaaataa ttattattat ttatttcttt gattaaaatc 1501 aaaagccttt aatgtatttt cttgatcttt catgcgctta acggtaaaat ccaaggtatt 1561 aagttcacct tccacttttc tctctctatc ttctctagaa aatcataaaa aatttatttg 1621 ctttgtgagc ggataacaat tataatagat tcaattgtga gcggataaca atttcacaca 1681 gaattcatta aagaggagaa attacatatg gataagaaat actcaatagg cttagctatc 1741 ggcacaaata gcgtcggatg ggcggtgatc actgatgaat ataaggttcc gtctaaaaag 1801 ttcaaggttc tgggaaatac agaccgccac agtatcaaaa aaaatcttat aggggctctt 1861 ttatttgaca gtggagagac agcggaagcg actcgtctca aacggacagc tcgtagaagg 1921 tatacacgtc ggaagaatcg tatttgttat ctacaggaga ttttttcaaa tgagatggcg 1981 aaagtagatg atagtttctt tcatcgactt gaagagtctt ttttggtgga agaagacaag 2041 aagcatgaac gtcatcctat ttttggaaat atagtagatg aagttgctta tcatgagaaa 2101 tatccaacta tctatcatct gcgaaaaaaa ttggtagatt ctactgataa agcggatttg 2161 cgcttaatct atttggcctt agcgcatatg attaagtttc gtggtcattt tttgattgag 2221 ggagatttaa atcctgataa tagtgatgtg gacaaactat ttatccagtt ggtacaaacc 2281 tacaatcaat tatttgaaga aaaccctatt aacgcaagtg gagtagatgc taaagcgatt 2341 ctttctgcac gattgagtaa atcaagacga ttagaaaatc tcattgctca gctccccggt 2401 gagaagaaaa atggcttatt tgggaatctc attgctttgt cattgggttt gacccctaat 2461 tttaaatcaa attttgattt ggcagaagat gctaaattac agctttcaaa agatacttac 2521 gatgatgatt tagataattt attggcgcaa attggagatc aatatgctga tttgtttttg 2581 gcagctaaga atttatcaga tgctatttta ctttcagata tcctaagagt aaatactgaa 2641 ataactaagg ctcccctatc agcttcaatg attaaacgct acgatgaaca tcatcaagac 2701 ttgactcttt taaaagcttt agttcgacaa caacttccag aaaagtataa agaaatcttt 2761 tttgatcaat caaaaaacgg atatgcaggt tatattgatg ggggagctag ccaagaagaa 2821 ttttataaat ttatcaaacc aattttagaa aaaatggatg gtactgagga attattggtg 2881 aaactaaatc gtgaagattt gctgcgcaag caacggacct ttgacaacgg ctctattccc 2941 catcaaattc acttgggtga gctgcatgct attttgagaa gacaagaaga cttttatcca 3001 tttttaaaag acaatcgtga gaagattgaa aaaatcttga cttttcgaat tccttattat 3061 gttggtccat tggcgcgtgg caatagtcgt tttgcatgga tgactcggaa gtctgaagaa 3121 acaattaccc catggaattt tgaagaagtt gtcgataaag gtgcttcagc tcaatcattt 3181 attgaacgca tgacaaactt tgataaaaat cttccaaatg aaaaagtact accaaaacat 3241 agtttgcttt atgagtattt tacggtttat aacgaattga caaaggtcaa atatgttact 3301 gaaggaatgc gaaaaccagc atttctttca ggtgaacaga agaaagccat tgttgattta 3361 ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa aagaagatta tttcaaaaaa 3421 atagaatgtt ttgatagtgt tgaaatttca ggagttgaag atagatttaa tgcttcatta 3481 ggtacctacc atgatttgct aaaaattatt aaagataaag attttttgga taatgaagaa 3541 aatgaagata tcttagagga tattgtttta acattgacct tatttgaaga tagggagatg 3601 attgaggaaa gacttaaaac atatgctcac ctctttgatg ataaggtgat gaaacagctt 3661 aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa aattgattaa tggtattagg 3721 gataagcaat ctggcaaaac aatattagat tttttgaaat cagatggttt tgccaatcgc 3781 aattttatgc agctgatcca tgatgatagt ttgacattta aagaagacat tcaaaaagca 3841 caagtgtctg gacaaggcga tagtttacat gaacatattg caaatttagc tggtagccct 3901 gctattaaaa aaggtatttt acagactgta aaagttgttg atgaattggt caaagtaatg 3961 gggcggcata agccagaaaa tatcgttatt gaaatggcac gtgaaaatca gacaactcaa 4021 aagggccaga aaaattcgcg agagcgtatg aaacgaatcg aagaaggtat caaagaatta 4081 ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc aattgcaaaa tgaaaagctc 4141 tatctctatt atctccaaaa tggaagagac atgtatgtgg accaagaatt agatattaat 4201 cgtttaagtg attatgatgt cgatgccatt gttccacaaa gtttccttaa agacgattca 4261 atagacaata aggtcttaac gcgttctgat aaaaatcgtg gtaaatcgga taacgttcca 4321 agtgaagaag tagtcaaaaa gatgaaaaac tattggagac aacttctaaa cgccaagtta 4381 atcactcaac gtaagtttga taatttaacg aaagctgaac gtggaggttt gagtgaactt 4441 gataaagctg gttttatcaa acgccaattg gttgaaactc gccaaatcac taagcatgtg 4501 gcacaaattt tggatagtcg catgaatact aaatacgatg aaaatgataa acttattcga 4561 gaggttaaag tgattacctt aaaatctaaa ttagtttctg acttccgaaa agatttccaa 4621 ttctataaag tacgtgagat taacaattac catcatgccc atgatgcgta tctaaatgcc 4681 gtcgttggaa ctgctttgat taagaaatat ccaaaacttg aatcggagtt tgtctatggt 4741 gattataaag tttatgatgt tcgtaaaatg attgctaagt ctgagcaaga aataggcaaa 4801 gcaaccgcaa aatatttctt ttactctaat atcatgaact tcttcaaaac agaaattaca 4861 cttgcaaatg gagagattcg caaacgccct ctaatcgaaa ctaatgggga aactggagaa 4921 attgtctggg ataaagggcg agattttgcc acagtgcgca aagtattgtc catgccccaa 4981 gtcaatattg tcaagaaaac agaagtccag acaggcggat tctccaagga gtcaatttta 5041 ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag actgggatcc aaaaaaatat 5101 ggtggttttg atagtccaac ggtagcttat tcagtcctag tggttgctaa ggtggaaaaa 5161 gggaaatcga agaagttaaa atccgttaaa gagttactag ggatcacaat tctggaaaga 5221 agttcctttg aaaaaaatcc gattgacttt ttagaagcta aaggatataa ggaagttaaa 5281 aaagacttaa tcattaaact acctaaatat agtctttttg agttagaaaa cggtcgtaaa 5341 cggatgctgg ctagtgccgg agaattacaa aaaggaaatg agctggctct gccaagcaaa 5401 tatgtgaatt ttttatattt agctagtcat tatgaaaagt tgaagggtag tccagaagat 5461 aacgaacaaa aacaattgtt tgtggagcag cataagcatt atttagatga gattattgag 5521 caaatcagtg aattttctaa gcgtgttatt ttagcagatg ccaatttaga taaagttctt 5581 agtgcatata acaaacatag agacaaacca atacgtgaac aagcagaaaa tattattcat 5641 ttatttacgt tgacgaatct tggagctccc gctgctttta aatattttga tacaacaatt 5701 gatcgtaaac gatatacgtc tacaaaagaa gttttagatg ccactcttat ccatcaatcc 5761 atcactggtc tttatgaaac acgcattgat ttgagtcagc taggaggtga ctaataaaag 5821 cttgatcaga tctcagcttt tttttgaagt gcctggcagt aagttgaaaa aatttgaaat 5881 aaacttggac tatgttaata aaaaaataca aattaacgaa caaaaagatt tgaattatta 5941 tataatttag ttaaaagcaa ttttaaatga gggaggtttc catatgaggg aagcggtgat 6001 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc 6061 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag 6121 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt 6181 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt 6241 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga 6301 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac 6361 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt 6421 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct 6481 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt 6541 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga 6601 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga 6661 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt 6721 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgacg 6781 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcatgacca aaatccctta 6841 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 6901 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 6961 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 7021 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 7081 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 7141 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 7201 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 7261 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 7321 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 7381 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 7441 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 7501 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcact 7561 agatccccgg caagttcatc cttagggttt tcatatctct taaaaagtca atttctgctt 7621 gattaatttc ttgtggatta ttgtttttgc ggtttttcat tttttatctc cttgagtatt 7681 gaattttttc ttatatataa atatatagca aaaactattt ttgccaactt ttttacaaaa 7741 aattttttat taaaacactt agggctttgc taaattctct tttaaaagaa cttagtaaag 7801 ccctatggat ttaagaactg attatactta cgtagtaaaa atactacaga tattaatagt 7861 atagaccata tcaaaatcat aatcaattta tatttatcta aaattgtagt ccatacaaaa 7921 tacaaaaaaa ttaggttttt ctaaattcta ttttaaaaga acttagtaac acctaatctc 7981 taaattactt acaaaattct aaaattagat ttttagttct tcatatttct ttaaaagttt 8041 tttaagaaaa tctttttgat tttcaaaaat ttcatgcatc ataaaactag taaatttgga 8101 tttttttttg taaaaatcat aactttcttg ggttttaagt tgaaatctta atggttttat 8161 tggattttgc ctagattttt ttaacgccgg actttcttta tcttttataa aattataaga 8221 atttttaata ccattttcta ttaaataatc ttcttttata agcccctctt gcagtgcagt 8281 tgctaatctt aaatacttat acgcttgagt ttttgctaca tcaaagtctt ttataaaatc 8341 aagaaaagtt ttataaccat caaatttata atattgatta tcttttattt cttttaaaat 8401 tttaatattt tccaccctgt ggaatatttc ttttttgaaa ttgtcattta acttttcctt 8461 tagtgtcttg taataagaaa ttttactttc attaatcatt aaattttgtt caacataagt 8521 atctatcttt tttgttaaaa ctttaacaac acgttttttt tccataaaaa ttttccttta 8581 tttttgcaac attttcctta attgatataa ttgttactat tttagatttt ttaatatcca 8641 cttaagaatt aatccctcac tagaatattt tttattatag aattcatata catgtttttc 8701 taaatctata aatttatcaa caattgcttt attatagggt gtatctatct tttcttttct 8761 taataatcgt ctcatattct ttaaataaca acaaacactg ccggttttaa acctaaattc 8821 tatgtaatat atttttgaaa aagtatatac attttcaacc ccgtttattt catactttac 8881 aaaaacatta tttattggtt ttctataacc atagtaaatg cctaaaaatt tgtccccgtc 8941 tttcatagga tatagataaa gtttttgaat ctctgattta tctaataatt tttcaaaaaa 9001 aattatacac cgcttttgtt tgttactaac tactctatgc aatgccttta atatttttgt 9061 gtgatatata tttttatcac cttttttttc tttcagcagg aagatacttt cttttggaaa 9121 tttaattttt tgttcattta ttcttgcagc cttaagtccg attttcatat ttaatcctta 9181 tatagccttt ctatttaatt cttttaaact caataaactt ttttggtttt tcattagttc 9241 taataattcg caataatatg tgcaaaacac tttattgtat tctattttct tttggttatt 9301 caaatattct tttataattg gctttagaag ttcaatattt acttcttttt ttagttgttc 9361 aataaggata ttgaaaatgt ttgtttttaa atcttgataa ttttgttgag aattttcttt 9421 ttttctttca attgacttct ctaatttacg ctttatattg tttaaatcgc tatatttatg 9481 actttcaata ataaaatgag gcttaaattt gtaattttcg tatatttttt ggaaacttgt 9541 ttctaattgt ttagaattat attcattttt ttcaaattct ttttgagtgt tgcatagaat 9601 ttcctttaat ttttcttgtt tttctttcat acaagattta gtaaaatgta tatttttatt 9661 ttttagtaat ttaatttcta ttatttttat gattttaagt attttgatta tattatcttt 9721 atcaatattt aaattgaata aaattgtaag aaaaattgaa agaatttttt tacataaaaa 9781 gttacattta ttgaagtaat ttcttacttg atacttttct atttcattaa tctttctttc 9841 ttcttttata ttatttttat tatttaaaca ctcctttaaa tctacactcc catttttagt 9901 aaatctatct ttaaggcccc ttgaaactct attttgaaat cttaaatctt ttttttcttg 9961 aaagtattta tagatttttt ggtaacattc tttttttgaa aaattaagtt gatagtatat 10021 ttcggtcccc aaatttaccc ctaagtgttt gtgataatta gttgtgactt taaattcttt 10081 ttctaatttg taaaggtaat tttgcagtgt ttttagttta acgggtgttt gactatttct 10141 tttaaggttt tcattaaagt aatagagtat gtttttttgt gtgtatttct tatatgtttt 10201 atttatgtac gctagtgttg agataaggac tactaattta tactgtaatt tattgtagca 10261 agttgtggtt ttactggttt tgtttatatt agataagttt tccatttata gctccttgaa 10321 taccggattt tttatctata aagtatatat atcacaattt tgatttaaaa gtaaattctt 10381 tttttaaaaa tacaaaaatt ttttataaat tatatagacc atgatattgt attaatacta 10441 attgatttta atttaaggtt gaactaaaat aaacatagtt ttgtagaaaa taatttttca 10501 ttattttgct acttgatatt ggatcgtaaa aatattaggg ctttacaaag ttcttttaaa 10561 agagaattta gtaaagccct aagtctttta ataaaaaatt ttttgtaaaa aagttggcaa 10621 aaatagtttt tgctatatat ttatatataa gaaaaaattc aatactcaag gagataaaaa 10681 atggaaaact taaacaataa tccacaaaaa attaatcaaa taaataagca aactaatttt 10741 attggttttg aaaattttat agaacttgat aatcaagtta atcaaactaa agaaaaaagt 10801 aaattaagta agataggtaa gaaattaccc ggaataagca gtcaagtcta gtgcggccgc 10861 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa 10921 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag 10981 ggcagtcgcc ctaaaacaaa gttaaacatc atgaggaggt aacgtcatgg taaaagattg 11041 gattcctatt tctcatgata attataaaca agtacaagga ccattttatc atggaactaa 11101 agctaattta gcaattggtg atttactaac tacaggattt atttctcatt ttgaagatgg 11161 tcgaattctt aaacatattt atttttcagc tttaatggaa ccagcagttt ggggagctga 11221 acttgctatg tcactatctg gtcttgaagg tcgtggttat atatatatag ttgaaccaac 11281 aggaccattt gaagatgatc caaatcttac aaata //