| 出品公司: | Novagen |
|---|---|
| 载体名称: | pIEx/Bac-1 |
| 质粒类型: | 昆虫细胞表达载体；蛋白纯化 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | IE-1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 7496 bp |
| 5' 测序引物及序列: | P10 |
| 3' 测序引物及序列: | -- |
| 载体标签: | C-His tag, N-GST tag |
| 载体抗性: | 氨苄青霉素（Ampicillin） |
| 筛选标记: | -- |
| 备注: | 含有双标签，可以采用两套纯化方案。 |
| 产品目录号: | #71727 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | 杆状病毒 |
买家导航
pIEx/Bac-4载体 pIEx-Bac-4质粒基本信息
pIEx/Bac-4质粒图谱 pIEx-Bac-4载体图谱和pIEx/Bac-4载体序列 pIEx-Bac-4质粒序列多克隆位点信息
pIEx/Bac-4质粒 pIEx-Bac-4载体简介
The pIEx/Bac-4 vector contains an N-terminal GST Tag coding sequence followed by a recognition site for enterokinase.
The multiple cloning region is followed by an optional C-terminal His Tag coding sequence. The pIEx/Bac vectors are
dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect
cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) enhancer/immediate early
promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV p10 very late
promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions to mediate
recombination at the polh locus.
pIEx/Bac-4质粒序列 pIEx-Bac-4载体序列
LOCUS pIEx/Bac-4 7496 bp DNA circular 22-AUG-2007
DEFINITION pREN4494: pIEx/Bac p10/AAT/Pseudo UTR/GST/EkLIC/10xHis.
SOURCE
ORGANISM
FEATURES Location/Qualifiers
promoter 1682..2228
/vntifkey="29"
/label=ie1\Promoter
misc_recomb complement(3616..5034)
/vntifkey="86"
/label=1629
rep_origin 6971..7426
/vntifkey="33"
/label=f1\origin
CDS complement(5982..6839)
/vntifkey="4"
/label=Ap
rep_origin complement(5221..5221)
/vntifkey="33"
/label=origin
misc_recomb 4..453
/vntifkey="86"
/label=lef-2
misc_recomb complement(491..1096)
/vntifkey="86"
/label=ORF\603
rep_origin 5054..5813
/vntifkey="33"
/label=pUC\Ori
enhancer 1196..1677
/vntifkey="9"
/label=hr5
polyA_signal 3442..3447
/vntifkey="25"
/label=PolyA\signal
misc_feature 3410..3413
/vntifkey="21"
/label=3x\-BseR\I\TCCT\to\CTTC
misc_feature 3179..3193
/vntifkey="21"
/label=C-Term\Ek/LIC\overhang
misc_binding 3203..3232
/vntifkey="20"
/label=10x\His-Tag
primer 3197..3245
/vntifkey="27"
/label=S-CTerm\10xHis
terminator 3265..3571
/vntifkey="43"
/label=ie1\Terminator
primer 3249..3262
/vntifkey="27"
/label=AS-Bsu36\I\Adapt\Avr
5'UTR 2229..2278
/vntifkey="52"
/label=ie1\5'-UTR
promoter 2279..2392
/vntifkey="29"
/label=p10\promoter\+\5'UTR
misc_feature 2277..2280
/vntifkey="21"
/label=Bsa\I\jct
CDS 2435..3094
/invitrogen="1170000"
/vntifkey="4"
/label=GST\tag\(no\stop)
misc_feature 2395..2434
/vntifkey="21"
/label=polH\pseudo\5'-UTR\ATT
protein_bind 3119..3133
/vntifkey="31"
/label=Ek\site
primer 2216..2237
/vntifkey="27"
/label=IE1\Promoter\Primer
primer 2325..2345
/vntifkey="27"
/label=p10\Promoter\primer
primer complement(3327..3345)
/vntifkey="27"
/label=IE1\Terminator\Primer
BASE COUNT 2030 a 1590 c 1655 g 2221 t
ORIGIN
1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
1141 tcaatatata gttgctgatc agatctgatc atggagataa ttaaaatgat aagggcgcgt
1201 aaaacacaat caagtatgag tcataagctg atgtcatgtt ttgcacacgg ctcataaccg
1261 aactggcttt acgagtagaa ttctacttgt aacgcacgat cagtggatga tgtcatttgt
1321 ttttcaaatc gagatgatgt catgttttgc acacggctca taaactcgct ttacgagtag
1381 aattctacgt gtaacgcacg atcgattgat gagtcatttg ttttgcaata tgatatcata
1441 caatatgact catttgtttt tcaaaaccga acttgattta cgggtagaat tctacttgta
1501 aagcacaatc aaaaagatga tgtcatttgt ttttcaaaac tgaactcgct ttacgagtag
1561 aattctacgt gtaaaacaca atcaagaaat gatgtcattt gttataaaaa taaaagctga
1621 tgtcatgttt tgcacatggc tcataactaa actcgcttta cgggtagaat tctacgcgcg
1681 tcgatgtctt tgtgatgcgc gcgacatttt tgtaggttat tgataaaatg aacggatacg
1741 ttgcccgaca ttatcattaa atccttggcg tagaatttgt cgggtccatt gtccgtgtgc
1801 gctagcatgc ccgtaacgga cctcgtactt ttggcttcaa aggttttgcg cacagacaaa
1861 atgtgccaca cttgcagctc tgcatgtgtg cgcgttacca caaatcccaa cggcgcagtg
1921 tacttgttgt atgcaaataa atctcgataa aggcgcggcg cgcgaatgca gctgatcacg
1981 tacgtccctc gtgttccgtt caaggacggt gttatcgacc tcagattaat gtttatcggc
2041 cgactgtttt cgtatccgct caccaaacgc gtttttgcat taacattgta tgtcggcgga
2101 tgttctatat ctaatttgaa taaataaacg ataaccgcgt tggttttaga gggcataata
2161 aaagaaatat tgttatcgtg ttcgccatta gggcagtata aattgacgtt catgttggat
2221 attgtttcag ttgcaagttg acactggcgg cgacaagatc gtgaacaacc aagtgactcc
2281 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
2341 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaatccgga
2401 ttattcatac cgtcccacca tcgggcgcgg atccatggcc cctatactag gttattggaa
2461 aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga
2521 agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg
2581 tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat
2641 ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg
2701 tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat
2761 tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat
2821 gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac
2881 ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg
2941 cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat
3001 tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac
3061 gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt ccgcgtcata atcaagcaga
3121 tgacgacgac aaggtaccgg atccgacgtc ggtcgacaag cttgcggccg caggagctcc
3181 gggcttctcc tcaatttccg ctcatcacca ccatcatcac catcaccacc actaagtgat
3241 taacctcacg acctaggcct caggttatac atatattttg aatttaatta attatacata
3301 tattttatat tatttttgtc ttttattatc gaggggccgt tgttggtgtg gggttttgca
3361 tagaaataac aatgggagtt ggcgacgttg ctgcgccaac accacctccc ttccctcctt
3421 tcatcatgta tctgtagata aaataaaata ttaaacctaa aaacaagacc gcgcctatca
3481 acaaaatgat aggcattaac ttgccgctga cgctgtcact aacgttggac gatttgccga
3541 ctaaaccttc atcgcccagt aaccaatcta ggtagctgag cgcatgcaag ctgatccggg
3601 ttattagtac atttattaag cgctagattc tgtgcgttgt tgatttacag acaattgttg
3661 tacgtatttt aataattcat taaatttata atctttaggg tggtatgtta gagcgaaaat
3721 caaatgattt tcagcgtctt tatatctgaa tttaaatatt aaatcctcaa tagatttgta
3781 aaataggttt cgattagttt caaacaaggg ttgtttttcc gaaccgatgg ctggactatc
3841 taatggattt tcgctcaacg ccacaaaact tgccaaatct tgtagcagca atctagcttt
3901 gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg acgtcgttca aaatattatg
3961 cgcttttgta tttctttcat cactgtcgtt agtgtacaat tgactcgacg taaacacgtt
4021 aaatagagct tggacatatt taacatcggg cgtgttagct ttattaggcc gattatcgtc
4081 gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac gattttgcca tagccacacg
4141 acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa tttgtagttg agctttttgg
4201 aattatttct gattgcgggc gtttttgggc gggtttcaat ctaactgtgc ccgattttaa
4261 ttcagacaac acgttagaaa gcgatggtgc aggcggtggt aacatttcag acggcaaatc
4321 tactaatggc ggcggtggtg gagctgatga taaatctacc atcggtggag gcgcaggcgg
4381 ggctggcggc ggaggcggag gcggaggtgg tggcggtgat gcagacggcg gtttaggctc
4441 aaatgtctct ttaggcaaca cagtcggcac ctcaactatt gtactggttt cgggcgccgt
4501 ttttggtttg accggtctga gacgagtgcg atttttttcg tttctaatag cttccaacaa
4561 ttgttgtctg tcgtctaaag gtgcagcggg ttgaggttcc gtcggcattg gtggagcggg
4621 cggcaattca gacatcgatg gtggtggtgg tggtggaggc gctggaatgt taggcacggg
4681 agaaggtggt ggcggcggtg ccgccggtat aatttgttct ggtttagttt gttcgcgcac
4741 gattgtgggc accggcgcag gcgccgctgg ctgcacaacg gaaggtcgtc tgcttcgagg
4801 cagcgcttgg ggtggtggca attcaatatt ataattggaa tacaaatcgt aaaaatctgc
4861 tataagcatt gtaatttcgc tatcgtttac cgtgccgata tttaacaacc gctcaatgta
4921 agcaattgta ttgtaaagag attgtctcaa gctcggatcg atcccgcacg ccgataacaa
4981 gccttttcat ttttactaca gcattgtagt ggcgagacac ttcgctgtcg tcgaggttta
5041 aacgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
5101 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
5161 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
5221 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
5281 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
5341 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
5401 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
5461 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
5521 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
5581 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
5641 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
5701 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
5761 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
5821 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
5881 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
5941 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
6001 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
6061 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
6121 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
6181 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
6241 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
6301 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
6361 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
6421 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
6481 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
6541 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
6601 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
6661 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
6721 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
6781 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
6841 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
6901 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
6961 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
7021 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
7081 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
7141 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
7201 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
7261 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
7321 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
7381 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttccca ttcgccattc
7441 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgcca
//
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pIEx/Bac-4质粒 pIEx-Bac-4载体应用举例
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